Garlic virus X
Average proteome isoelectric point is 7.03
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 6 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|O09499|O09499_9VIRU Helicase OS=Garlic virus X OX=150284 PE=4 SV=1
MM1 pKa = 6.78 TTVQKK6 pKa = 11.0 LLDD9 pKa = 4.13 QISDD13 pKa = 3.73 PNARR17 pKa = 11.84 AAYY20 pKa = 10.16 SQDD23 pKa = 3.41 CLSAAKK29 pKa = 10.15 NAATKK34 pKa = 11.03 AMTIAPYY41 pKa = 10.82 AVTQTEE47 pKa = 4.29 ALTLEE52 pKa = 4.81 RR53 pKa = 11.84 IGITTSPFATTSNTHH68 pKa = 6.16 AADD71 pKa = 4.2 KK72 pKa = 10.59 IIEE75 pKa = 4.22 NDD77 pKa = 3.33 CLEE80 pKa = 4.37 IIGRR84 pKa = 11.84 YY85 pKa = 8.47 LPKK88 pKa = 10.68 EE89 pKa = 4.03 PVTLIQLKK97 pKa = 9.75 RR98 pKa = 11.84 AKK100 pKa = 10.29 LHH102 pKa = 5.43 LLKK105 pKa = 10.58 RR106 pKa = 11.84 GPNTDD111 pKa = 2.38 IFQNYY116 pKa = 7.15 CHH118 pKa = 6.58 EE119 pKa = 4.73 PKK121 pKa = 10.52 DD122 pKa = 3.63 VLRR125 pKa = 11.84 FGLTNPHH132 pKa = 5.72 VCPVVNTSCAVISDD146 pKa = 4.03 TLHH149 pKa = 6.31 FMSSQQVWTLFKK161 pKa = 10.81 RR162 pKa = 11.84 NPKK165 pKa = 9.49 LQRR168 pKa = 11.84 LYY170 pKa = 9.82 GTLVLPVEE178 pKa = 5.0 ALNKK182 pKa = 10.18 LPSLFPEE189 pKa = 4.6 IYY191 pKa = 10.29 KK192 pKa = 10.57 LEE194 pKa = 4.36 YY195 pKa = 9.78 YY196 pKa = 10.35 DD197 pKa = 3.79 NHH199 pKa = 6.38 FAYY202 pKa = 9.97 MPGGHH207 pKa = 6.74 GGGAYY212 pKa = 8.55 IHH214 pKa = 7.06 SYY216 pKa = 8.85 GTLKK220 pKa = 10.24 WLSTAQIGTEE230 pKa = 4.13 SNHH233 pKa = 6.12 LSIEE237 pKa = 4.23 KK238 pKa = 10.15 IEE240 pKa = 4.33 TKK242 pKa = 10.12 AAHH245 pKa = 6.96 HH246 pKa = 6.53 IMLIQRR252 pKa = 11.84 RR253 pKa = 11.84 RR254 pKa = 11.84 PEE256 pKa = 4.56 LPWPLPPLWVFHH268 pKa = 6.55 ASDD271 pKa = 4.09 YY272 pKa = 10.96 VRR274 pKa = 11.84 LPEE277 pKa = 3.9 IFYY280 pKa = 10.1 PSEE283 pKa = 4.18 ANVQRR288 pKa = 11.84 AYY290 pKa = 9.87 PHH292 pKa = 6.03 VFIKK296 pKa = 10.56 RR297 pKa = 11.84 LQLYY301 pKa = 8.31 CFSVKK306 pKa = 10.2 AVSLRR311 pKa = 11.84 DD312 pKa = 3.18 IFAKK316 pKa = 9.72 IRR318 pKa = 11.84 QVIPTEE324 pKa = 4.06 DD325 pKa = 3.34 LSRR328 pKa = 11.84 FSTADD333 pKa = 4.48 LIRR336 pKa = 11.84 LANYY340 pKa = 10.44 LLFITGMQQTSDD352 pKa = 3.74 YY353 pKa = 10.41 QSPLMEE359 pKa = 4.71 SLLGKK364 pKa = 10.15 LCLSIRR370 pKa = 11.84 ARR372 pKa = 11.84 VRR374 pKa = 11.84 EE375 pKa = 4.06 FFQNLLGKK383 pKa = 9.96 SSYY386 pKa = 10.95 AALLTVTDD394 pKa = 3.49 VRR396 pKa = 11.84 PVYY399 pKa = 9.33 FTTKK403 pKa = 9.88 PIRR406 pKa = 11.84 RR407 pKa = 11.84 PICGQPWFDD416 pKa = 3.55 EE417 pKa = 4.76 AEE419 pKa = 4.38 VEE421 pKa = 4.7 SKK423 pKa = 10.48 PATPTITGYY432 pKa = 10.09 PNPSPDD438 pKa = 5.05 DD439 pKa = 4.15 LDD441 pKa = 4.19 LLAEE445 pKa = 4.52 FEE447 pKa = 4.23 QHH449 pKa = 5.0 EE450 pKa = 4.19 RR451 pKa = 11.84 AMMEE455 pKa = 4.36 EE456 pKa = 3.99 NCFTSKK462 pKa = 9.74 TSATANRR469 pKa = 11.84 AQNPQQNRR477 pKa = 11.84 EE478 pKa = 3.74 RR479 pKa = 11.84 VTPEE483 pKa = 3.61 KK484 pKa = 10.3 LAHH487 pKa = 6.48 SSDD490 pKa = 3.7 EE491 pKa = 4.04 SHH493 pKa = 6.06 TQITSSQGVNDD504 pKa = 3.99 HH505 pKa = 6.29 TWPTEE510 pKa = 3.6 QSQPTNTCKK519 pKa = 10.73 EE520 pKa = 3.9 ITDD523 pKa = 4.77 DD524 pKa = 3.68 EE525 pKa = 4.96 NMMDD529 pKa = 3.73 GHH531 pKa = 5.5 VTDD534 pKa = 4.25 WTDD537 pKa = 2.9 SDD539 pKa = 4.64 SDD541 pKa = 4.03 HH542 pKa = 7.63 SDD544 pKa = 3.24 EE545 pKa = 6.35 DD546 pKa = 4.39 IEE548 pKa = 4.55 TQPEE552 pKa = 4.32 TNLSQSIHH560 pKa = 7.54 DD561 pKa = 4.78 DD562 pKa = 3.86 LLPDD566 pKa = 4.2 EE567 pKa = 5.66 SDD569 pKa = 3.96 LPDD572 pKa = 4.33 LEE574 pKa = 4.9 SVADD578 pKa = 4.08 EE579 pKa = 4.65 QSDD582 pKa = 4.08 AEE584 pKa = 4.62 DD585 pKa = 4.39 NGDD588 pKa = 3.75 STIDD592 pKa = 3.65 QANLCNTTCDD602 pKa = 3.6 AEE604 pKa = 4.42 TDD606 pKa = 3.64 KK607 pKa = 11.4 SAQTPAEE614 pKa = 4.27 AKK616 pKa = 10.21 SSPKK620 pKa = 9.88 SASRR624 pKa = 11.84 VDD626 pKa = 4.0 LTPLDD631 pKa = 3.93 SDD633 pKa = 3.79 TVEE636 pKa = 3.8 ILRR639 pKa = 11.84 LHH641 pKa = 6.78 GFTNYY646 pKa = 9.77 KK647 pKa = 8.53 PQHH650 pKa = 6.67 DD651 pKa = 4.76 GDD653 pKa = 4.02 FQIAPVFYY661 pKa = 10.68 NHH663 pKa = 6.09 QACNLPLYY671 pKa = 10.76 DD672 pKa = 5.57 GITNSAQSKK681 pKa = 8.72 FIEE684 pKa = 4.25 MAHH687 pKa = 5.57 ALKK690 pKa = 10.46 RR691 pKa = 11.84 NCYY694 pKa = 10.31 NYY696 pKa = 9.74 ILEE699 pKa = 4.42 PRR701 pKa = 11.84 RR702 pKa = 11.84 ATAFMSDD709 pKa = 3.27 VKK711 pKa = 11.38 NNLTGLVLPKK721 pKa = 10.11 LDD723 pKa = 4.63 RR724 pKa = 11.84 DD725 pKa = 4.2 LLTSWVALSEE735 pKa = 4.15 NACRR739 pKa = 11.84 KK740 pKa = 9.44 VGLIVIHH747 pKa = 6.15 GAGGAGKK754 pKa = 10.05 SRR756 pKa = 11.84 ALQEE760 pKa = 3.94 ILRR763 pKa = 11.84 SNSIDD768 pKa = 3.48 TNQVNIIVPTINLAVDD784 pKa = 3.84 WKK786 pKa = 10.95 KK787 pKa = 10.79 KK788 pKa = 10.06 LPKK791 pKa = 9.34 IDD793 pKa = 3.65 PRR795 pKa = 11.84 RR796 pKa = 11.84 VMTFEE801 pKa = 4.09 KK802 pKa = 10.45 ACEE805 pKa = 3.99 RR806 pKa = 11.84 EE807 pKa = 4.42 GKK809 pKa = 10.11 SVVIMDD815 pKa = 5.34 DD816 pKa = 3.58 YY817 pKa = 11.94 GKK819 pKa = 10.9 LPAGYY824 pKa = 8.89 IDD826 pKa = 5.1 AYY828 pKa = 10.95 LAIKK832 pKa = 10.04 FNVEE836 pKa = 3.71 IVILTGDD843 pKa = 3.22 QRR845 pKa = 11.84 QSVFHH850 pKa = 7.11 NDD852 pKa = 3.21 RR853 pKa = 11.84 KK854 pKa = 10.63 DD855 pKa = 3.44 SQIALLTSNTAHH867 pKa = 7.2 FKK869 pKa = 10.47 RR870 pKa = 11.84 YY871 pKa = 9.29 CDD873 pKa = 3.61 YY874 pKa = 11.17 YY875 pKa = 11.6 LNATHH880 pKa = 6.81 RR881 pKa = 11.84 QPRR884 pKa = 11.84 RR885 pKa = 11.84 LANPIRR891 pKa = 11.84 VHH893 pKa = 6.64 AEE895 pKa = 3.83 RR896 pKa = 11.84 EE897 pKa = 3.84 VGGAIKK903 pKa = 10.33 HH904 pKa = 5.22 STLIPTHH911 pKa = 6.63 AMTLVPAFRR920 pKa = 11.84 SQSLLTDD927 pKa = 4.09 LGRR930 pKa = 11.84 QAMTYY935 pKa = 10.18 AGCQGLTLPHH945 pKa = 6.01 LTIVLDD951 pKa = 4.36 KK952 pKa = 10.04 DD953 pKa = 3.68 TPLCSDD959 pKa = 3.5 EE960 pKa = 4.38 VLYY963 pKa = 9.62 TALSRR968 pKa = 11.84 ASEE971 pKa = 4.34 TITFVNTYY979 pKa = 10.81 SNNKK983 pKa = 9.72 DD984 pKa = 3.54 FLDD987 pKa = 4.27 KK988 pKa = 11.06 LDD990 pKa = 3.66 STPYY994 pKa = 10.72 LKK996 pKa = 9.81 TLISGVRR1003 pKa = 11.84 EE1004 pKa = 3.99 DD1005 pKa = 4.62 EE1006 pKa = 4.17 QVGWEE1011 pKa = 4.16 NPPPEE1016 pKa = 4.8 PEE1018 pKa = 4.22 VRR1020 pKa = 11.84 DD1021 pKa = 3.97 APTKK1025 pKa = 7.99 THH1027 pKa = 6.83 IPVANNNVQLEE1038 pKa = 4.49 SKK1040 pKa = 10.32 IEE1042 pKa = 3.98 TMEE1045 pKa = 4.78 DD1046 pKa = 3.09 KK1047 pKa = 10.11 DD1048 pKa = 4.63 TRR1050 pKa = 11.84 EE1051 pKa = 3.87 LWSGEE1056 pKa = 3.84 EE1057 pKa = 4.18 KK1058 pKa = 10.4 TNLMQTQDD1066 pKa = 3.52 PVIQLFPHH1074 pKa = 5.38 QQAKK1078 pKa = 10.89 DD1079 pKa = 3.24 EE1080 pKa = 4.47 ALFKK1084 pKa = 10.13 ITINEE1089 pKa = 4.29 RR1090 pKa = 11.84 IKK1092 pKa = 10.87 LASPEE1097 pKa = 4.39 TNCKK1101 pKa = 9.64 ALKK1104 pKa = 8.4 EE1105 pKa = 4.16 TLNTGNLLFEE1115 pKa = 5.23 AYY1117 pKa = 10.21 ADD1119 pKa = 3.65 FMNVPRR1125 pKa = 11.84 EE1126 pKa = 3.97 TQPFDD1131 pKa = 3.22 KK1132 pKa = 10.56 RR1133 pKa = 11.84 LWAHH1137 pKa = 6.37 CRR1139 pKa = 11.84 QLALRR1144 pKa = 11.84 TYY1146 pKa = 10.54 LSKK1149 pKa = 9.75 PTTNLQQGAMQDD1161 pKa = 3.23 PDD1163 pKa = 3.78 FPEE1166 pKa = 3.79 NAIALFNKK1174 pKa = 8.34 SQWVKK1179 pKa = 10.35 KK1180 pKa = 10.25 LEE1182 pKa = 4.06 KK1183 pKa = 10.47 VGCKK1187 pKa = 9.95 FKK1189 pKa = 11.02 AGQTISAFKK1198 pKa = 10.55 QSTVLLTTTMALYY1211 pKa = 10.12 LRR1213 pKa = 11.84 KK1214 pKa = 9.53 KK1215 pKa = 10.33 RR1216 pKa = 11.84 EE1217 pKa = 3.63 SHH1219 pKa = 5.86 QPDD1222 pKa = 3.56 NVFVMCEE1229 pKa = 3.73 KK1230 pKa = 10.34 TPEE1233 pKa = 4.02 QFNSFVLTRR1242 pKa = 11.84 WDD1244 pKa = 3.69 FTRR1247 pKa = 11.84 PNYY1250 pKa = 9.75 TSDD1253 pKa = 3.13 YY1254 pKa = 8.03 TQYY1257 pKa = 11.34 DD1258 pKa = 3.52 QSQDD1262 pKa = 3.34 SAFLNFEE1269 pKa = 4.01 LRR1271 pKa = 11.84 KK1272 pKa = 9.85 AKK1274 pKa = 10.41 HH1275 pKa = 6.16 LGLPDD1280 pKa = 3.69 EE1281 pKa = 4.85 VVDD1284 pKa = 5.53 FYY1286 pKa = 12.15 AFIKK1290 pKa = 8.86 THH1292 pKa = 6.42 AKK1294 pKa = 9.19 TFLGNLAVMRR1304 pKa = 11.84 LSGEE1308 pKa = 4.14 GPTFDD1313 pKa = 6.1 ANTEE1317 pKa = 4.05 CNIAYY1322 pKa = 8.34 DD1323 pKa = 3.78 ALRR1326 pKa = 11.84 FQLDD1330 pKa = 3.55 STVNASYY1337 pKa = 11.04 AGDD1340 pKa = 3.71 DD1341 pKa = 3.79 LVRR1344 pKa = 11.84 DD1345 pKa = 4.02 RR1346 pKa = 11.84 ARR1348 pKa = 11.84 SEE1350 pKa = 4.04 RR1351 pKa = 11.84 PGWKK1355 pKa = 9.3 YY1356 pKa = 11.13 AEE1358 pKa = 4.31 PLFSLKK1364 pKa = 10.51 AKK1366 pKa = 10.13 PLVTSKK1372 pKa = 10.96 PDD1374 pKa = 3.17 FCGWRR1379 pKa = 11.84 LTKK1382 pKa = 10.71 YY1383 pKa = 10.46 GIVKK1387 pKa = 10.52 SPVQLYY1393 pKa = 10.28 QSLQLALRR1401 pKa = 11.84 LGKK1404 pKa = 10.19 VEE1406 pKa = 4.03 EE1407 pKa = 4.42 VKK1409 pKa = 10.49 RR1410 pKa = 11.84 SYY1412 pKa = 11.23 AIDD1415 pKa = 3.38 YY1416 pKa = 9.93 LFAYY1420 pKa = 10.43 RR1421 pKa = 11.84 LGDD1424 pKa = 3.38 QLYY1427 pKa = 10.56 DD1428 pKa = 3.39 IFDD1431 pKa = 3.81 EE1432 pKa = 5.39 DD1433 pKa = 4.24 EE1434 pKa = 4.2 LAKK1437 pKa = 10.5 HH1438 pKa = 5.31 QLVTRR1443 pKa = 11.84 TLIKK1447 pKa = 10.51 KK1448 pKa = 9.59 GMQPPSSGEE1457 pKa = 3.8 HH1458 pKa = 6.19 LPTFHH1463 pKa = 6.1 VTSDD1467 pKa = 3.01 RR1468 pKa = 11.84 LIRR1471 pKa = 11.84 DD1472 pKa = 3.8 PKK1474 pKa = 10.2 AIQVLSYY1481 pKa = 10.1 EE1482 pKa = 4.44 SDD1484 pKa = 3.59 RR1485 pKa = 11.84 IMVPTDD1491 pKa = 3.2 VIEE1494 pKa = 4.34 DD1495 pKa = 3.91 HH1496 pKa = 6.35 FTLASRR1502 pKa = 11.84 TDD1504 pKa = 3.83 RR1505 pKa = 11.84 DD1506 pKa = 3.96 DD1507 pKa = 3.14 MTAEE1511 pKa = 4.1 SNRR1514 pKa = 11.84 RR1515 pKa = 11.84 ASGLCTPINPGDD1527 pKa = 3.72 STGALLTDD1535 pKa = 4.26 MFPSIATLL1543 pKa = 3.41
Molecular weight: 174.31 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 5.661
IPC2_protein 5.588
IPC_protein 5.652
Toseland 6.02
ProMoST 5.982
Dawson 5.881
Bjellqvist 5.906
Wikipedia 5.881
Rodwell 5.868
Grimsley 6.16
Solomon 5.881
Lehninger 5.881
Nozaki 6.148
DTASelect 6.338
Thurlkill 6.326
EMBOSS 6.287
Sillero 6.237
Patrickios 4.151
IPC_peptide 5.906
IPC2_peptide 6.237
IPC2.peptide.svr19 6.203
Protein with the highest isoelectric point:
>tr|O09501|O09501_9VIRU 32 kDa protein OS=Garlic virus X OX=150284 PE=4 SV=1
MM1 pKa = 7.54 SFTPPPDD8 pKa = 3.28 HH9 pKa = 6.5 TKK11 pKa = 10.94 SFLALATGAAAGLLIYY27 pKa = 7.97 TLRR30 pKa = 11.84 SNQLAPVGDD39 pKa = 3.91 NTHH42 pKa = 5.94 QLPHH46 pKa = 6.52 GGRR49 pKa = 11.84 YY50 pKa = 8.86 RR51 pKa = 11.84 DD52 pKa = 3.45 GNKK55 pKa = 9.04 FVSYY59 pKa = 10.64 NGPNTGASSHH69 pKa = 6.08 SIWWPAITAITLTIVIHH86 pKa = 6.01 FLSSTRR92 pKa = 11.84 RR93 pKa = 11.84 RR94 pKa = 11.84 VCVRR98 pKa = 11.84 CSEE101 pKa = 4.3 STLPHH106 pKa = 6.36
Molecular weight: 11.53 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.21
IPC2_protein 9.385
IPC_protein 9.911
Toseland 9.736
ProMoST 9.677
Dawson 10.058
Bjellqvist 9.882
Wikipedia 10.306
Rodwell 10.16
Grimsley 10.175
Solomon 10.16
Lehninger 10.116
Nozaki 9.911
DTASelect 9.838
Thurlkill 9.882
EMBOSS 10.189
Sillero 10.014
Patrickios 7.556
IPC_peptide 10.145
IPC2_peptide 9.165
IPC2.peptide.svr19 8.12
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
6
0
6
2541
106
1543
423.5
47.55
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
7.477 ± 0.497
1.889 ± 0.334
5.864 ± 0.952
5.549 ± 0.557
4.211 ± 0.475
4.565 ± 0.735
3.109 ± 0.496
4.998 ± 0.266
5.195 ± 0.521
10.114 ± 0.687
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
1.85 ± 0.345
4.88 ± 0.389
6.061 ± 0.401
4.526 ± 0.439
5.549 ± 0.486
7.399 ± 0.651
8.107 ± 0.516
4.801 ± 0.372
0.826 ± 0.245
3.03 ± 0.299
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here