Oceanobacillus sp. 160

Taxonomy: cellular organisms; Bacteria; Terrabacteria group; Firmicutes; Bacilli; Bacillales; Bacillaceae; Oceanobacillus

Average proteome isoelectric point is 6.1

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 3747 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A345PHU1|A0A345PHU1_9BACI Uncharacterized protein OS=Oceanobacillus sp. 160 OX=2052660 GN=CUC15_11835 PE=4 SV=1
MM1 pKa = 8.24DD2 pKa = 5.95DD3 pKa = 3.99FFSPLVNVLKK13 pKa = 10.52IIYY16 pKa = 9.53DD17 pKa = 4.08SIATYY22 pKa = 10.86VIGTVIWIIEE32 pKa = 4.39LIRR35 pKa = 11.84NFLLDD40 pKa = 3.15TGIIDD45 pKa = 3.91NVITATVIAVAIIFIIFLVLVGWFLGPLRR74 pKa = 11.84VYY76 pKa = 10.9GGDD79 pKa = 3.64YY80 pKa = 11.08DD81 pKa = 5.86SDD83 pKa = 4.47DD84 pKa = 3.56NN85 pKa = 4.81

Molecular weight: 9.53 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A345PM28|A0A345PM28_9BACI Serine acetyltransferase OS=Oceanobacillus sp. 160 OX=2052660 GN=cysE PE=3 SV=1
MM1 pKa = 7.44KK2 pKa = 9.6RR3 pKa = 11.84TFQPNNRR10 pKa = 11.84KK11 pKa = 9.23RR12 pKa = 11.84KK13 pKa = 8.12KK14 pKa = 8.46VHH16 pKa = 5.5GFRR19 pKa = 11.84TRR21 pKa = 11.84MSTANGRR28 pKa = 11.84KK29 pKa = 8.32VLQRR33 pKa = 11.84RR34 pKa = 11.84RR35 pKa = 11.84RR36 pKa = 11.84KK37 pKa = 8.93GRR39 pKa = 11.84KK40 pKa = 8.7VLSAA44 pKa = 4.05

Molecular weight: 5.34 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

3747

0

3747

1080641

27

1851

288.4

32.37

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

7.011 ± 0.045

0.59 ± 0.011

5.25 ± 0.036

7.666 ± 0.051

4.543 ± 0.039

6.787 ± 0.04

1.953 ± 0.021

8.612 ± 0.047

6.671 ± 0.036

9.609 ± 0.048

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.704 ± 0.019

4.913 ± 0.029

3.483 ± 0.021

3.627 ± 0.03

3.805 ± 0.029

5.942 ± 0.032

5.467 ± 0.024

6.814 ± 0.031

0.99 ± 0.014

3.566 ± 0.023
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski