Pectobacterium phage Slant

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Autographiviridae; Corkvirinae; Phimunavirus; unclassified Phimunavirus

Average proteome isoelectric point is 6.32

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 55 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A385IGQ8|A0A385IGQ8_9CAUD Uncharacterized protein OS=Pectobacterium phage Slant OX=2320198 PE=4 SV=1
MM1 pKa = 7.23TKK3 pKa = 10.51YY4 pKa = 9.86IVSVPFCGYY13 pKa = 7.85MRR15 pKa = 11.84GYY17 pKa = 10.53KK18 pKa = 10.03VYY20 pKa = 9.52TVEE23 pKa = 4.36ADD25 pKa = 3.48SPEE28 pKa = 4.07EE29 pKa = 4.0AKK31 pKa = 10.76EE32 pKa = 4.07KK33 pKa = 10.73ACDD36 pKa = 3.1WDD38 pKa = 4.48YY39 pKa = 10.37ITEE42 pKa = 4.55SIEE45 pKa = 3.82VTRR48 pKa = 11.84DD49 pKa = 3.17DD50 pKa = 6.12SEE52 pKa = 4.54TDD54 pKa = 2.87WANAEE59 pKa = 4.24VAHH62 pKa = 6.83DD63 pKa = 3.78

Molecular weight: 7.26 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A385IH17|A0A385IH17_9CAUD HNHc domain-containing protein OS=Pectobacterium phage Slant OX=2320198 PE=4 SV=1
MM1 pKa = 7.87RR2 pKa = 11.84SRR4 pKa = 11.84VALEE8 pKa = 4.11LNQKK12 pKa = 9.54AVQAEE17 pKa = 4.61LKK19 pKa = 10.57AMYY22 pKa = 9.35YY23 pKa = 9.86RR24 pKa = 11.84KK25 pKa = 9.91AWRR28 pKa = 11.84CGMTLQAYY36 pKa = 7.69CARR39 pKa = 11.84FNVRR43 pKa = 11.84GIVV46 pKa = 3.12

Molecular weight: 5.4 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

55

0

55

13764

40

1263

250.3

27.62

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

9.554 ± 0.442

1.221 ± 0.2

6.234 ± 0.246

5.267 ± 0.366

3.008 ± 0.142

7.6 ± 0.283

1.969 ± 0.233

4.606 ± 0.18

4.57 ± 0.329

8.631 ± 0.256

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.645 ± 0.179

4.606 ± 0.334

3.967 ± 0.225

4.715 ± 0.432

5.42 ± 0.335

6.575 ± 0.332

6.437 ± 0.461

7.549 ± 0.228

1.366 ± 0.137

4.061 ± 0.287
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski