Rhinolophus gammaherpesvirus 1
Average proteome isoelectric point is 6.74
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 84 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A2Z5U691|A0A2Z5U691_9GAMA Tripartite terminase subunit 1 OS=Rhinolophus gammaherpesvirus 1 OX=2054179 GN=ORF10 PE=3 SV=1
MM1 pKa = 7.7 KK2 pKa = 10.64 LLLLLGLCGVVLVQLIQGQITNTPQTTNSTNPDD35 pKa = 3.39 GDD37 pKa = 4.04 STDD40 pKa = 3.17 ATLNNSSTVSAPRR53 pKa = 11.84 QVPVLYY59 pKa = 10.38 YY60 pKa = 10.33 LSPQWRR66 pKa = 11.84 LPLLLTGIFLALLTVLCVLWFFYY89 pKa = 10.93 LCLRR93 pKa = 11.84 FVVCPPCDD101 pKa = 3.7 GEE103 pKa = 4.92 DD104 pKa = 3.55 PQAEE108 pKa = 4.12 EE109 pKa = 4.48 EE110 pKa = 4.45 EE111 pKa = 4.43 EE112 pKa = 4.69 GEE114 pKa = 4.16 HH115 pKa = 7.05 SEE117 pKa = 4.25 TTLEE121 pKa = 4.21 SGLGEE126 pKa = 4.1 SQEE129 pKa = 4.07 GSEE132 pKa = 5.5 AEE134 pKa = 4.17 DD135 pKa = 3.49 AVV137 pKa = 3.69
Molecular weight: 14.91 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.854
IPC2_protein 3.884
IPC_protein 3.77
Toseland 3.605
ProMoST 3.91
Dawson 3.732
Bjellqvist 3.897
Wikipedia 3.63
Rodwell 3.617
Grimsley 3.528
Solomon 3.719
Lehninger 3.668
Nozaki 3.859
DTASelect 3.961
Thurlkill 3.643
EMBOSS 3.643
Sillero 3.884
Patrickios 0.299
IPC_peptide 3.719
IPC2_peptide 3.872
IPC2.peptide.svr19 3.822
Protein with the highest isoelectric point:
>tr|A0A2Z5UL29|A0A2Z5UL29_9GAMA Envelope glycoprotein B OS=Rhinolophus gammaherpesvirus 1 OX=2054179 GN=ORF11 PE=3 SV=1
MM1 pKa = 6.5 GTCVEE6 pKa = 4.32 KK7 pKa = 10.83 GKK9 pKa = 10.95 KK10 pKa = 9.38 NIYY13 pKa = 9.25 RR14 pKa = 11.84 VEE16 pKa = 3.91 EE17 pKa = 4.29 RR18 pKa = 11.84 EE19 pKa = 4.3 EE20 pKa = 3.73 MWHH23 pKa = 6.46 PKK25 pKa = 9.88 HH26 pKa = 6.98 IIYY29 pKa = 8.33 TPCIYY34 pKa = 10.81 SKK36 pKa = 10.47 ILNVVSLVKK45 pKa = 10.29 RR46 pKa = 11.84 VSLVKK51 pKa = 10.27 RR52 pKa = 11.84 VSLVKK57 pKa = 10.27 RR58 pKa = 11.84 VSLVKK63 pKa = 10.27 RR64 pKa = 11.84 VSLVKK69 pKa = 10.27 RR70 pKa = 11.84 VSLVKK75 pKa = 10.31 RR76 pKa = 11.84 VSLYY80 pKa = 11.11 SLNTYY85 pKa = 10.43 SVLSAMDD92 pKa = 3.53 RR93 pKa = 11.84 DD94 pKa = 4.45 GIFVFLYY101 pKa = 10.03 KK102 pKa = 10.53 RR103 pKa = 11.84 KK104 pKa = 10.04
Molecular weight: 12.2 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.281
IPC2_protein 9.794
IPC_protein 10.116
Toseland 10.555
ProMoST 10.189
Dawson 10.672
Bjellqvist 10.321
Wikipedia 10.833
Rodwell 11.184
Grimsley 10.73
Solomon 10.716
Lehninger 10.687
Nozaki 10.526
DTASelect 10.321
Thurlkill 10.555
EMBOSS 10.935
Sillero 10.584
Patrickios 10.906
IPC_peptide 10.716
IPC2_peptide 9.092
IPC2.peptide.svr19 8.639
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
84
0
84
37158
74
2843
442.4
49.81
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
4.855 ± 0.202
2.446 ± 0.175
4.836 ± 0.163
5.463 ± 0.394
4.998 ± 0.198
4.742 ± 0.18
2.777 ± 0.102
5.732 ± 0.173
5.568 ± 0.168
10.116 ± 0.286
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.468 ± 0.127
4.718 ± 0.171
5.999 ± 0.361
4.252 ± 0.213
4.182 ± 0.136
9.164 ± 0.26
6.655 ± 0.241
6.768 ± 0.171
1.009 ± 0.071
3.251 ± 0.14
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here