Streptococcus satellite phage Javan379
Average proteome isoelectric point is 6.54
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 13 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A4D5ZRD6|A0A4D5ZRD6_9VIRU HTH cro/C1-type domain-containing protein OS=Streptococcus satellite phage Javan379 OX=2558669 GN=JavanS379_0011 PE=4 SV=1
MM1 pKa = 7.4 IGNYY5 pKa = 9.7 LKK7 pKa = 10.7 NLRR10 pKa = 11.84 KK11 pKa = 9.32 RR12 pKa = 11.84 QNIKK16 pKa = 10.23 VVEE19 pKa = 4.06 LAKK22 pKa = 9.78 TVQVSQPYY30 pKa = 9.63 ISNIEE35 pKa = 3.86 NEE37 pKa = 3.92 KK38 pKa = 10.56 RR39 pKa = 11.84 YY40 pKa = 6.91 PTKK43 pKa = 10.88 DD44 pKa = 3.2 LFFKK48 pKa = 10.61 IIISIAEE55 pKa = 4.31 LSPLNYY61 pKa = 9.63 EE62 pKa = 4.45 VYY64 pKa = 10.76 CDD66 pKa = 4.14 LEE68 pKa = 4.06 LSKK71 pKa = 10.6 EE72 pKa = 4.12 QKK74 pKa = 10.04 KK75 pKa = 10.63 DD76 pKa = 2.89 IYY78 pKa = 10.68 IPDD81 pKa = 3.68 VLPEE85 pKa = 4.04 FWEE88 pKa = 5.03 KK89 pKa = 10.89 YY90 pKa = 10.2 SSTILKK96 pKa = 10.08 NINEE100 pKa = 4.21 YY101 pKa = 11.0 LDD103 pKa = 5.07 DD104 pKa = 4.39 EE105 pKa = 5.17 DD106 pKa = 5.71 EE107 pKa = 4.22 ITSFEE112 pKa = 4.11 DD113 pKa = 3.45 FKK115 pKa = 11.5 DD116 pKa = 4.37 YY117 pKa = 11.04 VSSWNIEE124 pKa = 3.83 DD125 pKa = 3.46 MAIFPEE131 pKa = 4.53 FTDD134 pKa = 4.53 FLDD137 pKa = 3.87 SKK139 pKa = 10.99 YY140 pKa = 11.12 GQDD143 pKa = 4.44 DD144 pKa = 3.55 YY145 pKa = 12.2 GEE147 pKa = 4.14 FLDD150 pKa = 3.9 YY151 pKa = 10.92 KK152 pKa = 10.61 DD153 pKa = 3.72 YY154 pKa = 11.55 SSYY157 pKa = 11.14 QDD159 pKa = 3.26 PEE161 pKa = 4.41 YY162 pKa = 10.51 IKK164 pKa = 10.92 SIVTDD169 pKa = 2.87 YY170 pKa = 10.46 WYY172 pKa = 10.86 QDD174 pKa = 3.41 FLTEE178 pKa = 4.07 FLDD181 pKa = 3.88 YY182 pKa = 11.16 FEE184 pKa = 4.73 NTSTIDD190 pKa = 4.09 GIPNPEE196 pKa = 3.93 YY197 pKa = 10.68 TEE199 pKa = 4.14 IMLEE203 pKa = 3.97 TLAPQEE209 pKa = 4.08 LEE211 pKa = 3.51 IYY213 pKa = 7.31 TTIKK217 pKa = 10.19 EE218 pKa = 4.13 LQHH221 pKa = 6.45 RR222 pKa = 11.84 KK223 pKa = 10.32 GIFDD227 pKa = 4.07 RR228 pKa = 11.84 YY229 pKa = 8.25 QFKK232 pKa = 9.14 NTDD235 pKa = 3.52 KK236 pKa = 11.64 NNLTDD241 pKa = 5.18 LSIIDD246 pKa = 4.49 DD247 pKa = 3.86 SEE249 pKa = 5.71 AIYY252 pKa = 11.05 KK253 pKa = 8.4 LTLDD257 pKa = 4.16 GKK259 pKa = 9.27 HH260 pKa = 6.25 LSSADD265 pKa = 3.27 IIALQTLINGIRR277 pKa = 11.84 YY278 pKa = 8.9 KK279 pKa = 10.69 RR280 pKa = 3.43
Molecular weight: 33.27 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 4.301
IPC2_protein 4.342
IPC_protein 4.304
Toseland 4.126
ProMoST 4.431
Dawson 4.266
Bjellqvist 4.418
Wikipedia 4.151
Rodwell 4.139
Grimsley 4.037
Solomon 4.266
Lehninger 4.215
Nozaki 4.368
DTASelect 4.558
Thurlkill 4.139
EMBOSS 4.164
Sillero 4.418
Patrickios 3.363
IPC_peptide 4.266
IPC2_peptide 4.406
IPC2.peptide.svr19 4.344
Protein with the highest isoelectric point:
>tr|A0A4D5ZLV8|A0A4D5ZLV8_9VIRU Uncharacterized protein OS=Streptococcus satellite phage Javan379 OX=2558669 GN=JavanS379_0002 PE=4 SV=1
MM1 pKa = 7.99 KK2 pKa = 9.16 ITQHH6 pKa = 5.97 IKK8 pKa = 8.98 QDD10 pKa = 3.16 GSIVYY15 pKa = 10.06 RR16 pKa = 11.84 SSIYY20 pKa = 10.7 LGIDD24 pKa = 3.12 QITGKK29 pKa = 10.06 QVTTKK34 pKa = 9.23 ITGRR38 pKa = 11.84 TKK40 pKa = 10.86 KK41 pKa = 9.71 EE42 pKa = 4.06 VKK44 pKa = 10.06 QKK46 pKa = 11.0 AQDD49 pKa = 3.3 AVIDD53 pKa = 4.07 FKK55 pKa = 11.51 INGSTRR61 pKa = 11.84 FQASTISTYY70 pKa = 11.16 EE71 pKa = 3.9 EE72 pKa = 4.49 LASLWWDD79 pKa = 3.4 NHH81 pKa = 3.94 KK82 pKa = 9.36 HH83 pKa = 4.09 TVKK86 pKa = 10.56 PNSQDD91 pKa = 2.89 ATKK94 pKa = 10.73 RR95 pKa = 11.84 LIDD98 pKa = 3.61 NHH100 pKa = 5.95 VLPLFGAYY108 pKa = 9.56 KK109 pKa = 10.2 LDD111 pKa = 3.65 KK112 pKa = 10.12 LTTPLIQNIVNKK124 pKa = 10.34 LADD127 pKa = 3.6 KK128 pKa = 9.52 TNKK131 pKa = 10.12 GEE133 pKa = 4.13 AGAYY137 pKa = 8.89 LHH139 pKa = 6.56 YY140 pKa = 11.05 DD141 pKa = 4.09 KK142 pKa = 11.05 IHH144 pKa = 6.58 ALNKK148 pKa = 10.18 RR149 pKa = 11.84 ILQYY153 pKa = 10.84 GVVMQAIPFNPARR166 pKa = 11.84 EE167 pKa = 4.24 VILPRR172 pKa = 11.84 NTKK175 pKa = 9.05 KK176 pKa = 10.8 ANRR179 pKa = 11.84 QKK181 pKa = 10.89 VKK183 pKa = 10.57 HH184 pKa = 5.76 FNNLEE189 pKa = 4.01 LKK191 pKa = 10.65 RR192 pKa = 11.84 FLDD195 pKa = 4.03 YY196 pKa = 11.21 LDD198 pKa = 4.46 SLNTSKK204 pKa = 10.76 YY205 pKa = 8.48 RR206 pKa = 11.84 YY207 pKa = 8.75 FYY209 pKa = 10.96 EE210 pKa = 3.79 VTLYY214 pKa = 11.08 KK215 pKa = 10.63 FILATGCRR223 pKa = 11.84 INEE226 pKa = 3.95 VLALEE231 pKa = 4.43 WSDD234 pKa = 3.56 IDD236 pKa = 4.22 LDD238 pKa = 4.0 SAVVHH243 pKa = 5.01 VTKK246 pKa = 10.16 TLNYY250 pKa = 8.35 KK251 pKa = 9.4 QEE253 pKa = 4.1 INSPKK258 pKa = 10.23 SKK260 pKa = 10.48 SSYY263 pKa = 10.28 RR264 pKa = 11.84 DD265 pKa = 2.84 IDD267 pKa = 3.35 IDD269 pKa = 3.81 NQTVTMLKK277 pKa = 9.66 QYY279 pKa = 10.52 KK280 pKa = 9.62 RR281 pKa = 11.84 RR282 pKa = 11.84 QTQEE286 pKa = 3.07 AWKK289 pKa = 10.2 LGQTEE294 pKa = 4.85 SIVFSDD300 pKa = 5.45 FINGYY305 pKa = 10.27 ANNRR309 pKa = 11.84 SLFTRR314 pKa = 11.84 LTTHH318 pKa = 6.89 FKK320 pKa = 10.02 RR321 pKa = 11.84 AKK323 pKa = 8.34 VPNIGFHH330 pKa = 5.98 GFRR333 pKa = 11.84 HH334 pKa = 4.87 THH336 pKa = 6.58 ASLLLNSGIPYY347 pKa = 10.0 KK348 pKa = 10.34 EE349 pKa = 3.8 LQHH352 pKa = 6.73 RR353 pKa = 11.84 LGHH356 pKa = 5.68 STLSMTMDD364 pKa = 3.58 IYY366 pKa = 11.68 SHH368 pKa = 6.98 LSKK371 pKa = 11.0 EE372 pKa = 4.25 NAKK375 pKa = 10.37 KK376 pKa = 10.38 AVSFYY381 pKa = 10.59 EE382 pKa = 3.87 MAMEE386 pKa = 5.16 SII388 pKa = 4.37
Molecular weight: 44.8 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.047
IPC2_protein 9.194
IPC_protein 9.077
Toseland 9.853
ProMoST 9.531
Dawson 10.072
Bjellqvist 9.721
Wikipedia 10.233
Rodwell 10.54
Grimsley 10.145
Solomon 10.087
Lehninger 10.043
Nozaki 9.823
DTASelect 9.721
Thurlkill 9.911
EMBOSS 10.262
Sillero 9.97
Patrickios 9.75
IPC_peptide 10.087
IPC2_peptide 8.17
IPC2.peptide.svr19 8.139
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
13
0
13
2678
55
479
206.0
23.83
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
5.452 ± 0.447
0.523 ± 0.127
5.601 ± 0.562
8.364 ± 0.713
4.294 ± 0.278
4.668 ± 0.365
1.755 ± 0.356
6.945 ± 0.458
8.701 ± 0.435
9.821 ± 0.545
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.166 ± 0.266
6.273 ± 0.54
3.174 ± 0.61
4.444 ± 0.418
4.742 ± 0.337
5.639 ± 0.441
6.535 ± 0.325
5.078 ± 0.493
0.859 ± 0.156
4.966 ± 0.538
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here