Lunatimonas lonarensis
Average proteome isoelectric point is 6.45
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 4825 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|R7ZS84|R7ZS84_9BACT Alpha-L-rhamnosidase OS=Lunatimonas lonarensis OX=1232681 GN=ADIS_2735 PE=4 SV=1
MM1 pKa = 7.67 KK2 pKa = 10.39 SLSIYY7 pKa = 9.14 INHH10 pKa = 7.52 GIFVLIAMVLLFFDD24 pKa = 4.42 FQAAKK29 pKa = 10.62 AQGFNNNEE37 pKa = 4.08 WIFGYY42 pKa = 10.69 CEE44 pKa = 4.08 GGEE47 pKa = 4.01 NNYY50 pKa = 10.64 LSFGKK55 pKa = 10.28 DD56 pKa = 2.56 GTARR60 pKa = 11.84 VRR62 pKa = 11.84 TLSPSVVLGKK72 pKa = 11.01 GNTAMAIDD80 pKa = 5.62 PISGDD85 pKa = 3.01 ILFYY89 pKa = 10.83 TDD91 pKa = 3.45 GALVYY96 pKa = 10.49 NYY98 pKa = 10.02 LNQPMQGIIGEE109 pKa = 4.31 LGGDD113 pKa = 3.22 EE114 pKa = 4.42 TGRR117 pKa = 11.84 QTVAIAVQEE126 pKa = 3.95 FDD128 pKa = 5.22 PIPGGSRR135 pKa = 11.84 SFYY138 pKa = 10.62 LFFINRR144 pKa = 11.84 RR145 pKa = 11.84 GEE147 pKa = 3.96 LEE149 pKa = 3.96 YY150 pKa = 11.19 DD151 pKa = 3.62 VVDD154 pKa = 3.99 MNNQGGAPASQPPAGAVSGGGILGTAQGGVAVIKK188 pKa = 9.71 TPASEE193 pKa = 4.0 SHH195 pKa = 6.91 LVFFDD200 pKa = 3.32 NGNLQVYY207 pKa = 10.03 RR208 pKa = 11.84 IEE210 pKa = 4.51 DD211 pKa = 3.27 QLGQFTLLQSEE222 pKa = 4.6 ALDD225 pKa = 3.7 FVPEE229 pKa = 3.99 AFVFDD234 pKa = 3.95 EE235 pKa = 4.8 GSEE238 pKa = 4.1 KK239 pKa = 10.55 FVVIPQGTGADD250 pKa = 3.93 LIVFDD255 pKa = 5.59 FDD257 pKa = 4.27 ASSGTLGNAQTLEE270 pKa = 4.21 NTGGDD275 pKa = 3.6 DD276 pKa = 4.58 PIFGVAFSPDD286 pKa = 2.65 GSMLYY291 pKa = 10.4 FSRR294 pKa = 11.84 GNQLFRR300 pKa = 11.84 IGADD304 pKa = 3.09 GSLADD309 pKa = 4.34 EE310 pKa = 4.65 LPEE313 pKa = 4.12 EE314 pKa = 4.46 EE315 pKa = 4.75 EE316 pKa = 4.98 EE317 pKa = 4.27 EE318 pKa = 4.21 DD319 pKa = 5.6 QEE321 pKa = 5.96 DD322 pKa = 4.28 EE323 pKa = 4.72 DD324 pKa = 5.09 PEE326 pKa = 4.43 ILTGPVEE333 pKa = 4.2 VALGHH338 pKa = 6.43 DD339 pKa = 4.3 LFRR342 pKa = 11.84 IYY344 pKa = 10.8 DD345 pKa = 3.77 LRR347 pKa = 11.84 VGPDD351 pKa = 3.03 GQLYY355 pKa = 9.76 YY356 pKa = 9.97 IYY358 pKa = 10.04 EE359 pKa = 4.14 EE360 pKa = 4.71 TEE362 pKa = 3.92 GGPQFLGRR370 pKa = 11.84 IVNPDD375 pKa = 3.15 EE376 pKa = 4.21 EE377 pKa = 4.89 ALGEE381 pKa = 4.22 LTVEE385 pKa = 4.48 ADD387 pKa = 3.52 PFEE390 pKa = 4.49 GTDD393 pKa = 3.44 FCGRR397 pKa = 11.84 VFTVFTPTIDD407 pKa = 3.22 VGPEE411 pKa = 3.35 VDD413 pKa = 4.75 FTWEE417 pKa = 3.92 PFDD420 pKa = 3.8 PCMNNPLLLTSQISPPNYY438 pKa = 9.54 RR439 pKa = 11.84 PVSFNWEE446 pKa = 3.91 ILPPLVDD453 pKa = 3.67 EE454 pKa = 5.24 DD455 pKa = 4.42 GEE457 pKa = 4.37 EE458 pKa = 4.42 IEE460 pKa = 4.39 MDD462 pKa = 3.64 LSKK465 pKa = 11.13 EE466 pKa = 3.9 HH467 pKa = 6.81 LLLPRR472 pKa = 11.84 EE473 pKa = 4.48 ATSEE477 pKa = 4.04 QQVTVVLTVTFANGEE492 pKa = 4.28 TRR494 pKa = 11.84 EE495 pKa = 4.12 VTQTINFQEE504 pKa = 4.43 NDD506 pKa = 3.52 LQVQFNPADD515 pKa = 3.66 TTLCDD520 pKa = 3.71 PACIDD525 pKa = 4.78 LMPLVDD531 pKa = 3.86 VQSGQDD537 pKa = 3.07 QGGQPGQPGVGQPGFGQPGFGQPGFGQPGFGQPGVGQPGFGQQPGQGGNYY587 pKa = 9.81 EE588 pKa = 4.32 YY589 pKa = 10.67 FWSNKK594 pKa = 9.62 RR595 pKa = 11.84 EE596 pKa = 4.29 DD597 pKa = 3.47 GWGPEE602 pKa = 4.27 APNEE606 pKa = 4.19 VCRR609 pKa = 11.84 PGYY612 pKa = 8.9 YY613 pKa = 8.62 WVLVRR618 pKa = 11.84 EE619 pKa = 4.47 VGSTCYY625 pKa = 10.18 SYY627 pKa = 11.6 AGIRR631 pKa = 11.84 IQMWDD636 pKa = 3.53 VQDD639 pKa = 3.35 QTNNIWYY646 pKa = 9.24 FGDD649 pKa = 3.94 GAGLDD654 pKa = 4.29 FNPDD658 pKa = 3.48 PDD660 pKa = 5.66 DD661 pKa = 4.66 PDD663 pKa = 5.29 APVPRR668 pKa = 11.84 PIPSRR673 pKa = 11.84 HH674 pKa = 6.05 PRR676 pKa = 11.84 NIPAGVTTVSDD687 pKa = 3.22 QAGQVLFFTDD697 pKa = 5.3 GEE699 pKa = 4.97 TVWDD703 pKa = 4.1 LNGNPMQNGLDD714 pKa = 3.39 IGGDD718 pKa = 3.68 PQSAGSVIAVPVASDD733 pKa = 3.13 EE734 pKa = 4.34 TLFYY738 pKa = 11.15 LFTTRR743 pKa = 11.84 RR744 pKa = 11.84 GADD747 pKa = 3.36 GNSVVSFSMVDD758 pKa = 2.94 IKK760 pKa = 11.46 GNNDD764 pKa = 3.45 DD765 pKa = 3.93 GVGNVVTKK773 pKa = 11.23 NNFLFSSSTEE783 pKa = 3.53 HH784 pKa = 6.88 SAALNAGDD792 pKa = 3.95 TTWVAFHH799 pKa = 6.73 EE800 pKa = 4.75 AGNNTFRR807 pKa = 11.84 MYY809 pKa = 10.17 PVSGQGIGQPVFSSVGGVHH828 pKa = 6.58 SFGSSSGVMKK838 pKa = 10.48 FSGDD842 pKa = 3.42 GSRR845 pKa = 11.84 LAITFQEE852 pKa = 4.07 GGMNKK857 pKa = 9.81 LQIFDD862 pKa = 4.25 FDD864 pKa = 4.11 QNTGEE869 pKa = 4.11 LTEE872 pKa = 4.6 FALLDD877 pKa = 4.31 LGTDD881 pKa = 3.3 GDD883 pKa = 4.41 VYY885 pKa = 11.32 GLEE888 pKa = 4.12 FSTDD892 pKa = 2.93 NTRR895 pKa = 11.84 VYY897 pKa = 10.65 VSYY900 pKa = 11.04 RR901 pKa = 11.84 NGGPGVEE908 pKa = 4.04 EE909 pKa = 5.07 FYY911 pKa = 10.99 LSSVEE916 pKa = 4.15 EE917 pKa = 4.41 TDD919 pKa = 6.09 DD920 pKa = 4.26 SDD922 pKa = 4.03 PDD924 pKa = 3.66 NPVTTRR930 pKa = 11.84 CQEE933 pKa = 4.14 CFDD936 pKa = 4.03 SAVEE940 pKa = 3.82 RR941 pKa = 11.84 RR942 pKa = 11.84 EE943 pKa = 3.74 IEE945 pKa = 3.96 QCILASRR952 pKa = 11.84 AAISQTGGLPLGALQVGPDD971 pKa = 3.56 GQIYY975 pKa = 9.1 VAVVGSNQIGQILPGRR991 pKa = 11.84 GCNPSTFNQDD1001 pKa = 2.41 AVEE1004 pKa = 4.11 PMPGTTNLGLPSFVQQSGSNIPEE1027 pKa = 4.2 PSLAGPNRR1035 pKa = 11.84 LCVGADD1041 pKa = 3.34 EE1042 pKa = 4.79 GAVGLFEE1049 pKa = 5.87 GGGEE1053 pKa = 4.09 PDD1055 pKa = 2.89 IDD1057 pKa = 4.14 LYY1059 pKa = 11.62 NWTIFNEE1066 pKa = 3.94 QGEE1069 pKa = 4.55 IVDD1072 pKa = 3.61 QFLNGAEE1079 pKa = 3.9 EE1080 pKa = 4.18 LQNLEE1085 pKa = 4.16 YY1086 pKa = 10.91 QFATAGLFTVQLEE1099 pKa = 4.4 VNRR1102 pKa = 11.84 CGEE1105 pKa = 3.81 PWDD1108 pKa = 3.98 EE1109 pKa = 4.08 IFRR1112 pKa = 11.84 LEE1114 pKa = 4.2 VEE1116 pKa = 4.71 VVDD1119 pKa = 4.16 SPEE1122 pKa = 5.24 IILPTDD1128 pKa = 2.99 IALCADD1134 pKa = 3.58 AVLEE1138 pKa = 4.26 LVAVDD1143 pKa = 4.25 PDD1145 pKa = 3.84 DD1146 pKa = 4.38 PRR1148 pKa = 11.84 LDD1150 pKa = 3.5 EE1151 pKa = 5.85 YY1152 pKa = 11.6 VFEE1155 pKa = 4.46 WVNAAGEE1162 pKa = 4.32 IVGDD1166 pKa = 3.83 QNVLEE1171 pKa = 4.27 VTEE1174 pKa = 4.07 EE1175 pKa = 3.96 SIYY1178 pKa = 10.55 TVTVAYY1184 pKa = 9.89 RR1185 pKa = 11.84 LPEE1188 pKa = 4.45 DD1189 pKa = 4.62 ADD1191 pKa = 3.42 PEE1193 pKa = 4.3 MFQTCPVSRR1202 pKa = 11.84 SVFVGPPFDD1211 pKa = 4.63 FDD1213 pKa = 3.43 IDD1215 pKa = 3.62 QSEE1218 pKa = 4.53 EE1219 pKa = 4.2 EE1220 pKa = 4.42 VCLGEE1225 pKa = 4.41 TVTFTPNSPVSGSWSIHH1242 pKa = 5.92 RR1243 pKa = 11.84 EE1244 pKa = 3.8 GSQDD1248 pKa = 4.1 RR1249 pKa = 11.84 ISLPDD1254 pKa = 3.66 TLQLTLNTAILGEE1267 pKa = 4.38 AGDD1270 pKa = 4.02 YY1271 pKa = 11.14 EE1272 pKa = 5.6 LFFLTSDD1279 pKa = 4.33 PLDD1282 pKa = 3.64 EE1283 pKa = 4.46 TCLFEE1288 pKa = 5.45 RR1289 pKa = 11.84 SAFLRR1294 pKa = 11.84 VNAGPQFEE1302 pKa = 5.06 VIATSPAQSCEE1313 pKa = 3.65 IGDD1316 pKa = 3.99 GVLEE1320 pKa = 4.13 IQAGVLLDD1328 pKa = 3.6 SLVVQEE1334 pKa = 4.32 TGQLFFDD1341 pKa = 4.36 VLPNDD1346 pKa = 4.19 IIVVDD1351 pKa = 4.08 GLEE1354 pKa = 4.06 PGIYY1358 pKa = 9.45 TLLGFANGCSSSQAAVIEE1376 pKa = 4.3 NANPPSDD1383 pKa = 3.51 VLFEE1387 pKa = 4.42 LEE1389 pKa = 4.75 TRR1391 pKa = 11.84 DD1392 pKa = 3.74 EE1393 pKa = 4.14 QCAPEE1398 pKa = 4.24 GLVAGGISILFGNGPASGVYY1418 pKa = 9.31 EE1419 pKa = 4.17 VLGLDD1424 pKa = 3.24 TGEE1427 pKa = 5.05 LISEE1431 pKa = 4.36 SFANQSVIALDD1442 pKa = 4.23 LPPGRR1447 pKa = 11.84 YY1448 pKa = 8.73 LVSVTDD1454 pKa = 3.44 VGGCEE1459 pKa = 3.85 VPAPSEE1465 pKa = 4.18 VIVNEE1470 pKa = 3.9 FSEE1473 pKa = 4.59 SVEE1476 pKa = 4.05 VEE1478 pKa = 3.97 LFSPNLCGPVVSTSITASGDD1498 pKa = 3.16 FLAVDD1503 pKa = 3.88 RR1504 pKa = 11.84 FGWFRR1509 pKa = 11.84 EE1510 pKa = 4.12 VNGEE1514 pKa = 4.35 FIPITGEE1521 pKa = 3.6 SGMVISVSEE1530 pKa = 3.94 PGRR1533 pKa = 11.84 YY1534 pKa = 7.73 EE1535 pKa = 3.54 VRR1537 pKa = 11.84 LYY1539 pKa = 11.47 NEE1541 pKa = 4.49 FDD1543 pKa = 4.31 CIIGSGVIDD1552 pKa = 4.45 VIQSATEE1559 pKa = 3.88 PPVLSPRR1566 pKa = 11.84 YY1567 pKa = 9.31 VICTLDD1573 pKa = 3.45 GNLAEE1578 pKa = 4.57 LQAGAGFASYY1588 pKa = 10.1 SWSLDD1593 pKa = 3.16 GQEE1596 pKa = 4.77 LGRR1599 pKa = 11.84 EE1600 pKa = 4.14 ARR1602 pKa = 11.84 FVPTLPGRR1610 pKa = 11.84 YY1611 pKa = 8.48 EE1612 pKa = 4.04 LLVTDD1617 pKa = 3.95 EE1618 pKa = 4.94 AGCEE1622 pKa = 3.8 FLVDD1626 pKa = 4.36 FEE1628 pKa = 4.76 VEE1630 pKa = 4.0 EE1631 pKa = 4.39 DD1632 pKa = 3.73 CEE1634 pKa = 4.63 INVRR1638 pKa = 11.84 FPNAIIPGSPTRR1650 pKa = 11.84 NFLLYY1655 pKa = 10.24 TRR1657 pKa = 11.84 GPIDD1661 pKa = 3.23 KK1662 pKa = 10.29 LGVYY1666 pKa = 9.74 IYY1668 pKa = 10.62 NRR1670 pKa = 11.84 WGEE1673 pKa = 4.05 LIYY1676 pKa = 11.0 YY1677 pKa = 10.01 CEE1679 pKa = 4.01 EE1680 pKa = 4.15 TNPSEE1685 pKa = 4.55 EE1686 pKa = 4.37 ISICTWDD1693 pKa = 4.26 GYY1695 pKa = 11.74 VNGQKK1700 pKa = 10.13 VPVGTYY1706 pKa = 8.52 PVVVKK1711 pKa = 9.01 FSNQQQQIEE1720 pKa = 4.32 RR1721 pKa = 11.84 TIRR1724 pKa = 11.84 RR1725 pKa = 11.84 AIVVIEE1731 pKa = 3.9
Molecular weight: 187.44 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.722
IPC2_protein 3.795
IPC_protein 3.808
Toseland 3.605
ProMoST 3.935
Dawson 3.783
Bjellqvist 3.935
Wikipedia 3.668
Rodwell 3.63
Grimsley 3.516
Solomon 3.783
Lehninger 3.732
Nozaki 3.884
DTASelect 4.075
Thurlkill 3.63
EMBOSS 3.681
Sillero 3.923
Patrickios 1.672
IPC_peptide 3.783
IPC2_peptide 3.91
IPC2.peptide.svr19 3.842
Protein with the highest isoelectric point:
>tr|R7ZTC9|R7ZTC9_9BACT Molybdenum transport system permease OS=Lunatimonas lonarensis OX=1232681 GN=ADIS_2253 PE=3 SV=1
MM1 pKa = 7.15 GRR3 pKa = 11.84 EE4 pKa = 3.77 KK5 pKa = 10.75 AHH7 pKa = 6.57 RR8 pKa = 11.84 SAIIVARR15 pKa = 11.84 GAAQPLVKK23 pKa = 10.02 NAHH26 pKa = 5.64 FRR28 pKa = 11.84 WVLAAWTVEE37 pKa = 3.97 EE38 pKa = 4.86 ASLFRR43 pKa = 11.84 QNPVLFGSALSRR55 pKa = 11.84 CLGPGTDD62 pKa = 4.29 VSPSSQGFWRR72 pKa = 11.84 EE73 pKa = 3.85 LVRR76 pKa = 11.84 GIAMVAAKK84 pKa = 9.3 TYY86 pKa = 10.16 LSEE89 pKa = 4.47 VVVKK93 pKa = 9.96 PRR95 pKa = 11.84 VEE97 pKa = 4.02 TRR99 pKa = 11.84 SYY101 pKa = 9.96 PGWVV105 pKa = 3.11
Molecular weight: 11.58 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.343
IPC2_protein 9.648
IPC_protein 10.555
Toseland 10.73
ProMoST 10.467
Dawson 10.818
Bjellqvist 10.555
Wikipedia 11.052
Rodwell 10.979
Grimsley 10.862
Solomon 10.979
Lehninger 10.935
Nozaki 10.716
DTASelect 10.54
Thurlkill 10.73
EMBOSS 11.14
Sillero 10.76
Patrickios 10.76
IPC_peptide 10.979
IPC2_peptide 9.589
IPC2.peptide.svr19 8.603
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
4825
0
4825
1591650
37
2922
329.9
37.08
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
7.086 ± 0.037
0.704 ± 0.011
5.446 ± 0.025
6.595 ± 0.036
5.204 ± 0.03
7.487 ± 0.035
2.048 ± 0.019
6.509 ± 0.03
5.62 ± 0.041
9.982 ± 0.038
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.427 ± 0.016
4.6 ± 0.035
4.325 ± 0.023
3.678 ± 0.017
5.07 ± 0.023
6.389 ± 0.025
5.081 ± 0.024
6.661 ± 0.027
1.382 ± 0.018
3.706 ± 0.021
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here