Roseburia sp. CAG:380

Taxonomy: cellular organisms; Bacteria; Terrabacteria group; Firmicutes; Clostridia; Eubacteriales; Lachnospiraceae; Roseburia; environmental samples

Average proteome isoelectric point is 6.13

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 2486 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|R6VN54|R6VN54_9FIRM Uncharacterized protein OS=Roseburia sp. CAG:380 OX=1262946 GN=BN635_01315 PE=4 SV=1
MM1 pKa = 7.13IQLNRR6 pKa = 11.84EE7 pKa = 3.78AGISQKK13 pKa = 10.7YY14 pKa = 10.01DD15 pKa = 2.64GSLDD19 pKa = 3.69YY20 pKa = 11.07GIEE23 pKa = 4.28MYY25 pKa = 11.03DD26 pKa = 3.2HH27 pKa = 7.27LPYY30 pKa = 10.32KK31 pKa = 10.53RR32 pKa = 11.84NSDD35 pKa = 3.76VCNAGAIALYY45 pKa = 9.79SGHH48 pKa = 6.84IIKK51 pKa = 10.75YY52 pKa = 6.65YY53 pKa = 9.74LCNNEE58 pKa = 4.04EE59 pKa = 3.7EE60 pKa = 4.92VYY62 pKa = 10.65PVDD65 pKa = 4.52NRR67 pKa = 11.84DD68 pKa = 4.54DD69 pKa = 4.63EE70 pKa = 4.51IQSILQRR77 pKa = 11.84ARR79 pKa = 11.84EE80 pKa = 3.92QAMQLQTAPEE90 pKa = 4.16EE91 pKa = 4.35PEE93 pKa = 4.54VIGDD97 pKa = 3.55PSEE100 pKa = 4.05ITDD103 pKa = 3.69EE104 pKa = 4.01QMQALTVEE112 pKa = 4.43QPEE115 pKa = 4.41TPEE118 pKa = 4.12DD119 pKa = 3.89AEE121 pKa = 4.64VSDD124 pKa = 4.51TEE126 pKa = 4.24QPAAPEE132 pKa = 4.23VVNLDD137 pKa = 5.58DD138 pKa = 5.25IEE140 pKa = 4.23DD141 pKa = 3.49WW142 pKa = 3.87

Molecular weight: 16.12 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|R6WEY4|R6WEY4_9FIRM Lactamase_B domain-containing protein OS=Roseburia sp. CAG:380 OX=1262946 GN=BN635_00926 PE=4 SV=1
MM1 pKa = 7.67KK2 pKa = 8.72MTFQPKK8 pKa = 8.51KK9 pKa = 8.23KK10 pKa = 10.11SRR12 pKa = 11.84ARR14 pKa = 11.84VHH16 pKa = 6.42GFRR19 pKa = 11.84KK20 pKa = 9.97RR21 pKa = 11.84MRR23 pKa = 11.84TANGRR28 pKa = 11.84KK29 pKa = 9.34VILSRR34 pKa = 11.84RR35 pKa = 11.84AKK37 pKa = 10.17GRR39 pKa = 11.84HH40 pKa = 5.5KK41 pKa = 10.9LSAA44 pKa = 3.8

Molecular weight: 5.21 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

2486

0

2486

809589

29

3340

325.7

36.51

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

7.481 ± 0.053

1.501 ± 0.022

5.92 ± 0.039

7.241 ± 0.06

3.81 ± 0.033

7.085 ± 0.046

1.803 ± 0.024

7.003 ± 0.052

6.903 ± 0.054

8.638 ± 0.05

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

3.172 ± 0.026

4.148 ± 0.039

3.246 ± 0.033

3.641 ± 0.03

4.673 ± 0.046

5.98 ± 0.042

5.611 ± 0.054

7.092 ± 0.037

0.879 ± 0.017

4.163 ± 0.033
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski