Amino acid dipepetide frequency for Roseburia sp. CAG:380

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10^-3.

For more information see sequence space article on Wikipedia.

	Ala	Cys	Asp	Glu	Phe	Gly	His	Ile	Lys	Leu	Met	Asn	Pro	Gln	Arg	Ser	Thr	Val	Trp	Tyr	Xaa
Ala	7.32AlaAla: 7.32 ± 0.123	1.104AlaCys: 1.104 ± 0.038	4.802AlaAsp: 4.802 ± 0.091	4.921AlaGlu: 4.921 ± 0.097	2.934AlaPhe: 2.934 ± 0.079	6.32AlaGly: 6.32 ± 0.112	1.098AlaHis: 1.098 ± 0.035	4.413AlaIle: 4.413 ± 0.084	5.087AlaLys: 5.087 ± 0.102	6.616AlaLeu: 6.616 ± 0.1	2.352AlaMet: 2.352 ± 0.055	2.427AlaAsn: 2.427 ± 0.064	2.112AlaPro: 2.112 ± 0.079	2.761AlaGln: 2.761 ± 0.06	2.879AlaArg: 2.879 ± 0.066	4.324AlaSer: 4.324 ± 0.086	3.349AlaThr: 3.349 ± 0.092	6.655AlaVal: 6.655 ± 0.1	0.639AlaTrp: 0.639 ± 0.028	2.688AlaTyr: 2.688 ± 0.058	0.007AlaXaa: 0.007 ± 0.003
Cys	0.945CysAla: 0.945 ± 0.036	0.287CysCys: 0.287 ± 0.022	0.899CysAsp: 0.899 ± 0.035	0.876CysGlu: 0.876 ± 0.035	0.665CysPhe: 0.665 ± 0.03	1.424CysGly: 1.424 ± 0.049	0.358CysHis: 0.358 ± 0.023	1.02CysIle: 1.02 ± 0.037	0.765CysLys: 0.765 ± 0.034	1.252CysLeu: 1.252 ± 0.038	0.506CysMet: 0.506 ± 0.023	0.595CysAsn: 0.595 ± 0.029	0.594CysPro: 0.594 ± 0.033	0.464CysGln: 0.464 ± 0.026	0.896CysArg: 0.896 ± 0.037	0.959CysSer: 0.959 ± 0.03	0.719CysThr: 0.719 ± 0.028	1.073CysVal: 1.073 ± 0.038	0.115CysTrp: 0.115 ± 0.012	0.597CysTyr: 0.597 ± 0.028	0.001CysXaa: 0.001 ± 0.001
Asp	4.384AspAla: 4.384 ± 0.082	0.795AspCys: 0.795 ± 0.034	3.302AspAsp: 3.302 ± 0.065	4.693AspGlu: 4.693 ± 0.086	2.464AspPhe: 2.464 ± 0.056	4.57AspGly: 4.57 ± 0.093	1.114AspHis: 1.114 ± 0.044	4.548AspIle: 4.548 ± 0.089	3.795AspLys: 3.795 ± 0.079	4.428AspLeu: 4.428 ± 0.072	1.955AspMet: 1.955 ± 0.047	2.431AspAsn: 2.431 ± 0.065	1.958AspPro: 1.958 ± 0.055	1.843AspGln: 1.843 ± 0.048	2.715AspArg: 2.715 ± 0.059	3.288AspSer: 3.288 ± 0.069	3.318AspThr: 3.318 ± 0.075	4.15AspVal: 4.15 ± 0.082	0.548AspTrp: 0.548 ± 0.028	2.903AspTyr: 2.903 ± 0.059	0.001AspXaa: 0.001 ± 0.001
Glu	4.996GluAla: 4.996 ± 0.087	0.862GluCys: 0.862 ± 0.035	4.26GluAsp: 4.26 ± 0.076	6.629GluGlu: 6.629 ± 0.115	2.291GluPhe: 2.291 ± 0.064	4.064GluGly: 4.064 ± 0.076	1.43GluHis: 1.43 ± 0.041	5.316GluIle: 5.316 ± 0.094	6.307GluLys: 6.307 ± 0.1	6.333GluLeu: 6.333 ± 0.105	2.405GluMet: 2.405 ± 0.059	3.775GluAsn: 3.775 ± 0.074	1.965GluPro: 1.965 ± 0.061	3.366GluGln: 3.366 ± 0.063	3.326GluArg: 3.326 ± 0.074	3.393GluSer: 3.393 ± 0.072	3.704GluThr: 3.704 ± 0.076	4.145GluVal: 4.145 ± 0.078	0.646GluTrp: 0.646 ± 0.03	3.195GluTyr: 3.195 ± 0.064	0.0GluXaa: 0.0 ± 0.0
Phe	2.919PheAla: 2.919 ± 0.068	0.77PheCys: 0.77 ± 0.033	2.421PheAsp: 2.421 ± 0.049	2.426PheGlu: 2.426 ± 0.051	1.563PhePhe: 1.563 ± 0.054	2.859PheGly: 2.859 ± 0.063	0.835PheHis: 0.835 ± 0.035	2.402PheIle: 2.402 ± 0.058	1.766PheLys: 1.766 ± 0.046	3.582PheLeu: 3.582 ± 0.084	1.147PheMet: 1.147 ± 0.039	1.27PheAsn: 1.27 ± 0.037	1.225PhePro: 1.225 ± 0.044	1.344PheGln: 1.344 ± 0.038	1.903PheArg: 1.903 ± 0.049	2.569PheSer: 2.569 ± 0.066	2.143PheThr: 2.143 ± 0.058	2.856PheVal: 2.856 ± 0.06	0.398PheTrp: 0.398 ± 0.028	1.696PheTyr: 1.696 ± 0.048	0.001PheXaa: 0.001 ± 0.001
Gly	4.988GlyAla: 4.988 ± 0.101	1.278GlyCys: 1.278 ± 0.047	3.666GlyAsp: 3.666 ± 0.079	4.69GlyGlu: 4.69 ± 0.071	2.857GlyPhe: 2.857 ± 0.062	4.742GlyGly: 4.742 ± 0.106	1.243GlyHis: 1.243 ± 0.04	5.73GlyIle: 5.73 ± 0.089	5.502GlyLys: 5.502 ± 0.092	5.473GlyLeu: 5.473 ± 0.089	2.663GlyMet: 2.663 ± 0.056	3.044GlyAsn: 3.044 ± 0.073	1.206GlyPro: 1.206 ± 0.04	2.463GlyGln: 2.463 ± 0.065	3.388GlyArg: 3.388 ± 0.075	4.444GlySer: 4.444 ± 0.075	4.411GlyThr: 4.411 ± 0.097	5.075GlyVal: 5.075 ± 0.09	0.651GlyTrp: 0.651 ± 0.029	3.329GlyTyr: 3.329 ± 0.061	0.009GlyXaa: 0.009 ± 0.004
His	1.12HisAla: 1.12 ± 0.04	0.338HisCys: 0.338 ± 0.024	0.913HisAsp: 0.913 ± 0.03	0.994HisGlu: 0.994 ± 0.033	0.865HisPhe: 0.865 ± 0.031	1.356HisGly: 1.356 ± 0.042	0.457HisHis: 0.457 ± 0.034	1.366HisIle: 1.366 ± 0.046	0.965HisLys: 0.965 ± 0.036	1.529HisLeu: 1.529 ± 0.05	0.582HisMet: 0.582 ± 0.025	0.745HisAsn: 0.745 ± 0.033	0.835HisPro: 0.835 ± 0.035	0.646HisGln: 0.646 ± 0.025	0.989HisArg: 0.989 ± 0.037	1.023HisSer: 1.023 ± 0.039	0.98HisThr: 0.98 ± 0.032	1.32HisVal: 1.32 ± 0.039	0.19HisTrp: 0.19 ± 0.015	0.818HisTyr: 0.818 ± 0.032	0.0HisXaa: 0.0 ± 0.0
Ile	5.541IleAla: 5.541 ± 0.084	1.367IleCys: 1.367 ± 0.038	4.069IleAsp: 4.069 ± 0.08	4.202IleGlu: 4.202 ± 0.074	2.659IlePhe: 2.659 ± 0.063	4.935IleGly: 4.935 ± 0.093	1.325IleHis: 1.325 ± 0.042	4.481IleIle: 4.481 ± 0.1	3.771IleLys: 3.771 ± 0.07	6.575IleLeu: 6.575 ± 0.112	1.876IleMet: 1.876 ± 0.049	2.794IleAsn: 2.794 ± 0.067	2.995IlePro: 2.995 ± 0.06	2.138IleGln: 2.138 ± 0.061	4.05IleArg: 4.05 ± 0.087	4.842IleSer: 4.842 ± 0.087	4.124IleThr: 4.124 ± 0.084	4.943IleVal: 4.943 ± 0.088	0.562IleTrp: 0.562 ± 0.028	2.778IleTyr: 2.778 ± 0.065	0.001IleXaa: 0.001 ± 0.001
Lys	5.297LysAla: 5.297 ± 0.095	0.752LysCys: 0.752 ± 0.039	4.224LysAsp: 4.224 ± 0.078	6.006LysGlu: 6.006 ± 0.103	1.726LysPhe: 1.726 ± 0.05	4.284LysGly: 4.284 ± 0.073	1.098LysHis: 1.098 ± 0.037	4.496LysIle: 4.496 ± 0.085	6.622LysLys: 6.622 ± 0.137	5.854LysLeu: 5.854 ± 0.081	2.242LysMet: 2.242 ± 0.053	3.34LysAsn: 3.34 ± 0.069	2.055LysPro: 2.055 ± 0.07	2.647LysGln: 2.647 ± 0.065	3.204LysArg: 3.204 ± 0.067	3.523LysSer: 3.523 ± 0.073	3.754LysThr: 3.754 ± 0.077	4.373LysVal: 4.373 ± 0.091	0.626LysTrp: 0.626 ± 0.029	2.904LysTyr: 2.904 ± 0.065	0.0LysXaa: 0.0 ± 0.0
Leu	6.484LeuAla: 6.484 ± 0.099	1.504LeuCys: 1.504 ± 0.042	4.907LeuAsp: 4.907 ± 0.089	6.197LeuGlu: 6.197 ± 0.092	3.65LeuPhe: 3.65 ± 0.083	5.549LeuGly: 5.549 ± 0.102	1.656LeuHis: 1.656 ± 0.049	5.472LeuIle: 5.472 ± 0.105	5.772LeuLys: 5.772 ± 0.087	8.087LeuLeu: 8.087 ± 0.148	2.537LeuMet: 2.537 ± 0.062	3.341LeuAsn: 3.341 ± 0.075	3.305LeuPro: 3.305 ± 0.088	3.258LeuGln: 3.258 ± 0.069	3.979LeuArg: 3.979 ± 0.071	5.938LeuSer: 5.938 ± 0.091	5.184LeuThr: 5.184 ± 0.09	5.573LeuVal: 5.573 ± 0.084	0.72LeuTrp: 0.72 ± 0.032	3.254LeuTyr: 3.254 ± 0.073	0.009LeuXaa: 0.009 ± 0.003
Met	2.326MetAla: 2.326 ± 0.055	0.338MetCys: 0.338 ± 0.017	1.966MetAsp: 1.966 ± 0.049	2.515MetGlu: 2.515 ± 0.066	0.982MetPhe: 0.982 ± 0.034	2.115MetGly: 2.115 ± 0.057	0.506MetHis: 0.506 ± 0.026	2.269MetIle: 2.269 ± 0.057	2.601MetLys: 2.601 ± 0.057	2.731MetLeu: 2.731 ± 0.064	0.973MetMet: 0.973 ± 0.036	1.544MetAsn: 1.544 ± 0.043	1.119MetPro: 1.119 ± 0.035	1.18MetGln: 1.18 ± 0.036	1.371MetArg: 1.371 ± 0.04	1.875MetSer: 1.875 ± 0.046	2.026MetThr: 2.026 ± 0.045	2.045MetVal: 2.045 ± 0.062	0.225MetTrp: 0.225 ± 0.015	1.012MetTyr: 1.012 ± 0.039	0.001MetXaa: 0.001 ± 0.001
Asn	3.149AsnAla: 3.149 ± 0.063	0.646AsnCys: 0.646 ± 0.027	2.239AsnAsp: 2.239 ± 0.053	2.691AsnGlu: 2.691 ± 0.062	1.362AsnPhe: 1.362 ± 0.042	3.625AsnGly: 3.625 ± 0.081	0.771AsnHis: 0.771 ± 0.032	3.312AsnIle: 3.312 ± 0.078	2.636AsnLys: 2.636 ± 0.064	3.272AsnLeu: 3.272 ± 0.064	1.349AsnMet: 1.349 ± 0.043	1.732AsnAsn: 1.732 ± 0.061	1.698AsnPro: 1.698 ± 0.048	1.365AsnGln: 1.365 ± 0.047	2.044AsnArg: 2.044 ± 0.045	2.192AsnSer: 2.192 ± 0.052	2.36AsnThr: 2.36 ± 0.057	3.008AsnVal: 3.008 ± 0.071	0.347AsnTrp: 0.347 ± 0.019	1.684AsnTyr: 1.684 ± 0.043	0.0AsnXaa: 0.0 ± 0.0
Pro	2.691ProAla: 2.691 ± 0.076	0.403ProCys: 0.403 ± 0.024	2.368ProAsp: 2.368 ± 0.057	3.246ProGlu: 3.246 ± 0.071	1.348ProPhe: 1.348 ± 0.047	2.228ProGly: 2.228 ± 0.059	0.539ProHis: 0.539 ± 0.027	1.831ProIle: 1.831 ± 0.047	1.991ProLys: 1.991 ± 0.051	2.532ProLeu: 2.532 ± 0.055	0.918ProMet: 0.918 ± 0.035	1.124ProAsn: 1.124 ± 0.036	0.662ProPro: 0.662 ± 0.039	1.122ProGln: 1.122 ± 0.04	0.908ProArg: 0.908 ± 0.037	1.952ProSer: 1.952 ± 0.067	1.768ProThr: 1.768 ± 0.129	3.203ProVal: 3.203 ± 0.073	0.288ProTrp: 0.288 ± 0.02	1.336ProTyr: 1.336 ± 0.041	0.001ProXaa: 0.001 ± 0.001
Gln	2.548GlnAla: 2.548 ± 0.06	0.371GlnCys: 0.371 ± 0.025	1.921GlnAsp: 1.921 ± 0.052	3.145GlnGlu: 3.145 ± 0.065	1.107GlnPhe: 1.107 ± 0.039	2.284GlnGly: 2.284 ± 0.052	0.535GlnHis: 0.535 ± 0.028	2.877GlnIle: 2.877 ± 0.062	3.179GlnLys: 3.179 ± 0.065	2.93GlnLeu: 2.93 ± 0.063	1.407GlnMet: 1.407 ± 0.044	1.812GlnAsn: 1.812 ± 0.051	1.018GlnPro: 1.018 ± 0.039	1.537GlnGln: 1.537 ± 0.057	1.601GlnArg: 1.601 ± 0.045	1.97GlnSer: 1.97 ± 0.053	2.127GlnThr: 2.127 ± 0.058	2.141GlnVal: 2.141 ± 0.057	0.348GlnTrp: 0.348 ± 0.023	1.553GlnTyr: 1.553 ± 0.041	0.002GlnXaa: 0.002 ± 0.002
Arg	2.595ArgAla: 2.595 ± 0.058	0.641ArgCys: 0.641 ± 0.034	2.542ArgAsp: 2.542 ± 0.074	3.862ArgGlu: 3.862 ± 0.073	1.905ArgPhe: 1.905 ± 0.053	2.611ArgGly: 2.611 ± 0.07	0.904ArgHis: 0.904 ± 0.036	3.786ArgIle: 3.786 ± 0.072	3.846ArgLys: 3.846 ± 0.08	4.096ArgLeu: 4.096 ± 0.087	1.676ArgMet: 1.676 ± 0.047	2.06ArgAsn: 2.06 ± 0.049	1.309ArgPro: 1.309 ± 0.041	2.078ArgGln: 2.078 ± 0.06	2.669ArgArg: 2.669 ± 0.074	2.443ArgSer: 2.443 ± 0.06	2.38ArgThr: 2.38 ± 0.05	2.842ArgVal: 2.842 ± 0.059	0.437ArgTrp: 0.437 ± 0.023	2.043ArgTyr: 2.043 ± 0.051	0.0ArgXaa: 0.0 ± 0.0
Ser	4.499SerAla: 4.499 ± 0.093	0.867SerCys: 0.867 ± 0.036	3.694SerAsp: 3.694 ± 0.081	3.858SerGlu: 3.858 ± 0.079	2.678SerPhe: 2.678 ± 0.059	5.279SerGly: 5.279 ± 0.088	1.057SerHis: 1.057 ± 0.038	3.932SerIle: 3.932 ± 0.079	3.377SerLys: 3.377 ± 0.076	5.211SerLeu: 5.211 ± 0.089	1.942SerMet: 1.942 ± 0.047	2.218SerAsn: 2.218 ± 0.055	1.714SerPro: 1.714 ± 0.05	1.927SerGln: 1.927 ± 0.053	2.841SerArg: 2.841 ± 0.059	4.018SerSer: 4.018 ± 0.109	3.015SerThr: 3.015 ± 0.071	4.579SerVal: 4.579 ± 0.088	0.498SerTrp: 0.498 ± 0.025	2.595SerTyr: 2.595 ± 0.06	0.001SerXaa: 0.001 ± 0.001
Thr	4.793ThrAla: 4.793 ± 0.099	0.646ThrCys: 0.646 ± 0.035	3.483ThrAsp: 3.483 ± 0.065	3.696ThrGlu: 3.696 ± 0.073	2.008ThrPhe: 2.008 ± 0.052	4.83ThrGly: 4.83 ± 0.094	0.854ThrHis: 0.854 ± 0.033	3.95ThrIle: 3.95 ± 0.066	3.576ThrLys: 3.576 ± 0.082	4.795ThrLeu: 4.795 ± 0.088	1.57ThrMet: 1.57 ± 0.042	2.017ThrAsn: 2.017 ± 0.051	2.432ThrPro: 2.432 ± 0.083	1.785ThrGln: 1.785 ± 0.052	2.018ThrArg: 2.018 ± 0.053	3.157ThrSer: 3.157 ± 0.076	3.223ThrThr: 3.223 ± 0.083	4.658ThrVal: 4.658 ± 0.101	0.499ThrTrp: 0.499 ± 0.027	2.115ThrTyr: 2.115 ± 0.058	0.004ThrXaa: 0.004 ± 0.002
Val	5.095ValAla: 5.095 ± 0.087	1.229ValCys: 1.229 ± 0.041	4.247ValAsp: 4.247 ± 0.081	4.329ValGlu: 4.329 ± 0.072	2.885ValPhe: 2.885 ± 0.069	4.184ValGly: 4.184 ± 0.084	1.155ValHis: 1.155 ± 0.037	5.456ValIle: 5.456 ± 0.084	4.554ValLys: 4.554 ± 0.092	6.445ValLeu: 6.445 ± 0.098	2.142ValMet: 2.142 ± 0.057	2.882ValAsn: 2.882 ± 0.063	2.628ValPro: 2.628 ± 0.083	2.273ValGln: 2.273 ± 0.045	3.178ValArg: 3.178 ± 0.067	4.883ValSer: 4.883 ± 0.084	4.728ValThr: 4.728 ± 0.109	5.198ValVal: 5.198 ± 0.095	0.627ValTrp: 0.627 ± 0.032	2.794ValTyr: 2.794 ± 0.058	0.006ValXaa: 0.006 ± 0.003
Trp	0.41TrpAla: 0.41 ± 0.024	0.147TrpCys: 0.147 ± 0.014	0.551TrpAsp: 0.551 ± 0.032	0.599TrpGlu: 0.599 ± 0.029	0.346TrpPhe: 0.346 ± 0.023	0.623TrpGly: 0.623 ± 0.032	0.193TrpHis: 0.193 ± 0.016	0.642TrpIle: 0.642 ± 0.028	0.792TrpLys: 0.792 ± 0.036	0.842TrpLeu: 0.842 ± 0.037	0.319TrpMet: 0.319 ± 0.021	0.521TrpAsn: 0.521 ± 0.025	0.172TrpPro: 0.172 ± 0.014	0.374TrpGln: 0.374 ± 0.023	0.385TrpArg: 0.385 ± 0.023	0.511TrpSer: 0.511 ± 0.027	0.436TrpThr: 0.436 ± 0.024	0.442TrpVal: 0.442 ± 0.024	0.114TrpTrp: 0.114 ± 0.012	0.373TrpTyr: 0.373 ± 0.021	0.0TrpXaa: 0.0 ± 0.0
Tyr	2.703TyrAla: 2.703 ± 0.065	0.665TyrCys: 0.665 ± 0.029	2.726TyrAsp: 2.726 ± 0.068	2.834TyrGlu: 2.834 ± 0.063	1.802TyrPhe: 1.802 ± 0.045	2.968TyrGly: 2.968 ± 0.07	0.903TyrHis: 0.903 ± 0.034	2.73TyrIle: 2.73 ± 0.06	2.12TyrLys: 2.12 ± 0.055	3.788TyrLeu: 3.788 ± 0.072	1.156TyrMet: 1.156 ± 0.037	1.808TyrAsn: 1.808 ± 0.06	1.448TyrPro: 1.448 ± 0.04	1.84TyrGln: 1.84 ± 0.053	2.373TyrArg: 2.373 ± 0.053	2.495TyrSer: 2.495 ± 0.058	2.356TyrThr: 2.356 ± 0.069	2.637TyrVal: 2.637 ± 0.066	0.314TyrTrp: 0.314 ± 0.02	1.969TyrTyr: 1.969 ± 0.051	0.0TyrXaa: 0.0 ± 0.0
Xaa	0.007XaaAla: 0.007 ± 0.003	0.0XaaCys: 0.0 ± 0.0	0.001XaaAsp: 0.001 ± 0.001	0.002XaaGlu: 0.002 ± 0.002	0.0XaaPhe: 0.0 ± 0.0	0.001XaaGly: 0.001 ± 0.001	0.0XaaHis: 0.0 ± 0.0	0.001XaaIle: 0.001 ± 0.001	0.002XaaLys: 0.002 ± 0.002	0.004XaaLeu: 0.004 ± 0.002	0.002XaaMet: 0.002 ± 0.001	0.002XaaAsn: 0.002 ± 0.002	0.005XaaPro: 0.005 ± 0.002	0.001XaaGln: 0.001 ± 0.001	0.009XaaArg: 0.009 ± 0.003	0.001XaaSer: 0.001 ± 0.001	0.004XaaThr: 0.004 ± 0.002	0.001XaaVal: 0.001 ± 0.001	0.0XaaTrp: 0.0 ± 0.0	0.0XaaTyr: 0.0 ± 0.0	0.059XaaXaa: 0.059 ± 0.013

Statistics based on 2486 proteins (809589 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944

Contact: Lukasz P. Kozlowski

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