Photobacterium ganghwense

Taxonomy: cellular organisms; Bacteria; Proteobacteria; Gammaproteobacteria; Vibrionales; Vibrionaceae; Photobacterium

Average proteome isoelectric point is 6.11

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 4693 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A0J1GY41|A0A0J1GY41_9GAMM ABC transporter OS=Photobacterium ganghwense OX=320778 GN=ABT57_23705 PE=4 SV=1
MM1 pKa = 7.56KK2 pKa = 8.99KK3 pKa = 8.34TVVAAVIGSVVASASAMAAEE23 pKa = 5.17VYY25 pKa = 10.57KK26 pKa = 10.92SDD28 pKa = 3.89DD29 pKa = 3.64LSVNIYY35 pKa = 10.0GNLRR39 pKa = 11.84VRR41 pKa = 11.84YY42 pKa = 7.01EE43 pKa = 4.07TTDD46 pKa = 3.37NGIMGADD53 pKa = 3.43DD54 pKa = 3.81TFTGEE59 pKa = 4.12GTEE62 pKa = 4.52LGLNMTYY69 pKa = 10.82FMPNGLYY76 pKa = 11.04VNGDD80 pKa = 3.5VLKK83 pKa = 9.66EE84 pKa = 3.88LNIIPEE90 pKa = 4.66DD91 pKa = 3.74GNGFDD96 pKa = 5.73DD97 pKa = 5.0EE98 pKa = 4.95EE99 pKa = 4.28FTKK102 pKa = 10.63FASAAIGGDD111 pKa = 3.18FGEE114 pKa = 4.27FRR116 pKa = 11.84IGRR119 pKa = 11.84MSAVQDD125 pKa = 3.88TLAGQYY131 pKa = 10.68DD132 pKa = 3.94ITWEE136 pKa = 4.05YY137 pKa = 11.3GGTANIKK144 pKa = 9.96NDD146 pKa = 2.85WSATDD151 pKa = 3.35RR152 pKa = 11.84LTNGVQYY159 pKa = 10.9KK160 pKa = 9.51FEE162 pKa = 4.97KK163 pKa = 10.83NGFTVMAQYY172 pKa = 8.28HH173 pKa = 6.02TGQEE177 pKa = 3.9YY178 pKa = 10.7DD179 pKa = 4.04DD180 pKa = 4.71RR181 pKa = 11.84DD182 pKa = 3.75GVISGDD188 pKa = 3.78IIEE191 pKa = 4.91IDD193 pKa = 3.05QAYY196 pKa = 9.97ALGVAWEE203 pKa = 4.31SDD205 pKa = 3.44FGLGLSGTYY214 pKa = 9.18TSSEE218 pKa = 3.82RR219 pKa = 11.84TEE221 pKa = 3.82KK222 pKa = 10.96DD223 pKa = 3.24KK224 pKa = 11.8NFGDD228 pKa = 5.02DD229 pKa = 5.81KK230 pKa = 11.49DD231 pKa = 5.3DD232 pKa = 3.63SWTLNATYY240 pKa = 10.36AWNSLSLAAMYY251 pKa = 10.68SDD253 pKa = 4.23YY254 pKa = 11.58DD255 pKa = 3.63FAGDD259 pKa = 3.52EE260 pKa = 4.18QTGVGAMVRR269 pKa = 11.84YY270 pKa = 9.11DD271 pKa = 3.45FPIGVGLYY279 pKa = 10.09GVYY282 pKa = 10.3DD283 pKa = 3.75YY284 pKa = 11.97VDD286 pKa = 4.16YY287 pKa = 8.82DD288 pKa = 3.95TKK290 pKa = 11.68DD291 pKa = 3.14NTLDD295 pKa = 3.47QFTVGADD302 pKa = 3.3YY303 pKa = 10.72WLTSQVVSYY312 pKa = 9.99MEE314 pKa = 4.24YY315 pKa = 11.09ADD317 pKa = 3.6MSYY320 pKa = 11.15DD321 pKa = 3.79EE322 pKa = 5.49EE323 pKa = 4.22KK324 pKa = 10.3TAIDD328 pKa = 4.16GQKK331 pKa = 10.31MMDD334 pKa = 4.05DD335 pKa = 3.62SAFTIGMRR343 pKa = 11.84LYY345 pKa = 10.62FF346 pKa = 4.33

Molecular weight: 38.3 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A0J1HGW1|A0A0J1HGW1_9GAMM MarR family transcriptional regulator OS=Photobacterium ganghwense OX=320778 GN=ABT57_05215 PE=4 SV=1
MM1 pKa = 7.43PRR3 pKa = 11.84QVIKK7 pKa = 11.0RR8 pKa = 11.84MMPSHH13 pKa = 6.28EE14 pKa = 4.15VIKK17 pKa = 10.33RR18 pKa = 11.84QKK20 pKa = 9.88ALKK23 pKa = 10.18IFGNVLYY30 pKa = 10.72NPNLWCLNRR39 pKa = 11.84RR40 pKa = 11.84SASGAFAVGLFMAFVPLPSQMIMAAGLAILFSVNLPLSVCLVWVSNPITMPVLFYY95 pKa = 10.88GAYY98 pKa = 9.8KK99 pKa = 10.2IGAWLLNTPHH109 pKa = 6.57QSFHH113 pKa = 7.33FEE115 pKa = 4.14LSWDD119 pKa = 3.62FLMSQMNQIGPPFLLGCMICGVGCALVGYY148 pKa = 9.35FGIRR152 pKa = 11.84GLWRR156 pKa = 11.84YY157 pKa = 9.89SVVRR161 pKa = 11.84SWQKK165 pKa = 10.2RR166 pKa = 11.84KK167 pKa = 9.73FRR169 pKa = 11.84VLRR172 pKa = 4.07

Molecular weight: 19.59 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

4693

0

4693

1473747

29

2939

314.0

34.77

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

9.442 ± 0.037

1.099 ± 0.013

5.437 ± 0.027

6.079 ± 0.032

3.946 ± 0.023

7.082 ± 0.032

2.35 ± 0.018

5.865 ± 0.027

4.595 ± 0.028

10.563 ± 0.044

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.812 ± 0.019

3.797 ± 0.025

4.273 ± 0.022

4.644 ± 0.031

5.052 ± 0.03

6.184 ± 0.026

5.421 ± 0.024

7.082 ± 0.029

1.305 ± 0.015

2.972 ± 0.021
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski