Raphanus sativus cryptic virus 3

Taxonomy: Viruses; Riboviria; Orthornavirae; Pisuviricota; Duplopiviricetes; Durnavirales; Partitiviridae; unclassified Partitiviridae

Average proteome isoelectric point is 7.11

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 2 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|B8Y0L2|B8Y0L2_9VIRU Uncharacterized protein OS=Raphanus sativus cryptic virus 3 OX=580254 PE=4 SV=1
MM1 pKa = 7.63EE2 pKa = 5.6NGANNPPAAGAIVAAVQPALPPGLDD27 pKa = 3.06ASKK30 pKa = 10.88LNRR33 pKa = 11.84ATEE36 pKa = 4.05YY37 pKa = 10.86LYY39 pKa = 10.96DD40 pKa = 4.18ADD42 pKa = 4.64KK43 pKa = 10.87IRR45 pKa = 11.84PHH47 pKa = 6.93RR48 pKa = 11.84ARR50 pKa = 11.84SPKK53 pKa = 8.91YY54 pKa = 9.43RR55 pKa = 11.84RR56 pKa = 11.84YY57 pKa = 8.56MQLKK61 pKa = 8.69KK62 pKa = 10.86NEE64 pKa = 4.33IYY66 pKa = 10.77VQLIDD71 pKa = 3.74IYY73 pKa = 10.35RR74 pKa = 11.84ASFSVTWPDD83 pKa = 3.63FRR85 pKa = 11.84HH86 pKa = 6.74LIHH89 pKa = 7.36AFPPPANHH97 pKa = 6.9EE98 pKa = 4.05PQTYY102 pKa = 9.0YY103 pKa = 10.59ACCYY107 pKa = 8.63ISGWFRR113 pKa = 11.84DD114 pKa = 3.94LYY116 pKa = 9.02YY117 pKa = 10.6HH118 pKa = 6.66IRR120 pKa = 11.84KK121 pKa = 8.97ALKK124 pKa = 10.04SLSGLAEE131 pKa = 3.82LEE133 pKa = 4.46YY134 pKa = 11.1YY135 pKa = 10.25RR136 pKa = 11.84DD137 pKa = 5.14PIPQMSFEE145 pKa = 4.3YY146 pKa = 10.98DD147 pKa = 2.99NFLALLGAAIRR158 pKa = 11.84PTHH161 pKa = 6.03IVGNPEE167 pKa = 3.64DD168 pKa = 4.08VMYY171 pKa = 11.09VPLISEE177 pKa = 4.25ATDD180 pKa = 3.32VNQVNPFGINNYY192 pKa = 6.72VHH194 pKa = 6.22QPEE197 pKa = 4.69LFSAIVATMKK207 pKa = 10.45DD208 pKa = 2.92RR209 pKa = 11.84KK210 pKa = 9.38KK211 pKa = 11.11FNMVPLPSNYY221 pKa = 9.77SGRR224 pKa = 11.84PSWLFDD230 pKa = 2.79WHH232 pKa = 8.36AEE234 pKa = 4.09DD235 pKa = 5.12QICAPFQPEE244 pKa = 4.23GNFNNEE250 pKa = 4.32DD251 pKa = 3.0IAMAFVLGTACTSLIGMRR269 pKa = 11.84DD270 pKa = 3.2RR271 pKa = 11.84DD272 pKa = 3.46DD273 pKa = 3.19WQFYY277 pKa = 10.21PNANVPAHH285 pKa = 6.31FNPHH289 pKa = 4.43TAQRR293 pKa = 11.84ITPRR297 pKa = 11.84TMYY300 pKa = 10.41GSYY303 pKa = 9.83EE304 pKa = 3.95VRR306 pKa = 11.84SYY308 pKa = 9.95EE309 pKa = 3.96TDD311 pKa = 2.77HH312 pKa = 7.59DD313 pKa = 4.81YY314 pKa = 11.5YY315 pKa = 10.55IPSNSAGVTAFLSGPSTSRR334 pKa = 11.84KK335 pKa = 9.37RR336 pKa = 11.84SRR338 pKa = 11.84TTSSSTPGSLVICKK352 pKa = 8.28YY353 pKa = 9.88ICLYY357 pKa = 10.06PYY359 pKa = 10.03RR360 pKa = 11.84LHH362 pKa = 6.25YY363 pKa = 10.67LPVHH367 pKa = 6.26INISLFNN374 pKa = 4.14

Molecular weight: 42.7 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|B8Y0L2|B8Y0L2_9VIRU Uncharacterized protein OS=Raphanus sativus cryptic virus 3 OX=580254 PE=4 SV=1
MM1 pKa = 7.65RR2 pKa = 11.84RR3 pKa = 11.84LRR5 pKa = 11.84NPLAGYY11 pKa = 10.03VYY13 pKa = 10.34TGFISDD19 pKa = 4.97LEE21 pKa = 4.38EE22 pKa = 3.84TNQRR26 pKa = 11.84HH27 pKa = 4.57TNVIRR32 pKa = 11.84RR33 pKa = 11.84EE34 pKa = 3.99TAVTYY39 pKa = 10.36RR40 pKa = 11.84DD41 pKa = 3.41EE42 pKa = 4.67FALQSILDD50 pKa = 3.85LDD52 pKa = 3.74YY53 pKa = 11.15RR54 pKa = 11.84QYY56 pKa = 10.73EE57 pKa = 3.81QHH59 pKa = 5.84LQGWSRR65 pKa = 11.84SYY67 pKa = 8.77YY68 pKa = 9.83TPEE71 pKa = 3.52KK72 pKa = 9.38HH73 pKa = 6.19QEE75 pKa = 4.06AILQYY80 pKa = 9.7SYY82 pKa = 11.54PDD84 pKa = 3.44IPVQAIKK91 pKa = 10.09MDD93 pKa = 3.59VYY95 pKa = 10.97NDD97 pKa = 4.95CIAQVQNEE105 pKa = 4.3FRR107 pKa = 11.84SLPNVRR113 pKa = 11.84AYY115 pKa = 10.8SVLTQLDD122 pKa = 3.78LVKK125 pKa = 10.76YY126 pKa = 9.32KK127 pKa = 10.67SSSAAGYY134 pKa = 9.85GYY136 pKa = 10.34QGTKK140 pKa = 10.36GNPGEE145 pKa = 4.45LNHH148 pKa = 6.61ARR150 pKa = 11.84AISRR154 pKa = 11.84AKK156 pKa = 10.13AVLWSAIAADD166 pKa = 4.09GEE168 pKa = 4.75GIEE171 pKa = 4.97HH172 pKa = 6.85VIKK175 pKa = 10.13TSTPDD180 pKa = 2.66IGYY183 pKa = 7.85TRR185 pKa = 11.84TQLTDD190 pKa = 3.31LTEE193 pKa = 4.12KK194 pKa = 10.31TKK196 pKa = 10.31VRR198 pKa = 11.84QVWGRR203 pKa = 11.84AFHH206 pKa = 6.55YY207 pKa = 10.25ILLEE211 pKa = 4.02GLVADD216 pKa = 4.76PFIQAVMEE224 pKa = 4.41NNTFIHH230 pKa = 7.04AGRR233 pKa = 11.84DD234 pKa = 3.53PTLSVPQLLSHH245 pKa = 7.19IAEE248 pKa = 4.3TSDD251 pKa = 3.36WIYY254 pKa = 11.51SLDD257 pKa = 3.31WKK259 pKa = 11.01QFDD262 pKa = 3.62ATVSRR267 pKa = 11.84FEE269 pKa = 4.17IEE271 pKa = 4.08SAFTIIKK278 pKa = 10.16SKK280 pKa = 9.88TIFPDD285 pKa = 3.54RR286 pKa = 11.84EE287 pKa = 4.01TEE289 pKa = 4.01DD290 pKa = 3.23AFEE293 pKa = 3.95ITKK296 pKa = 10.32QLFIHH301 pKa = 6.42KK302 pKa = 9.64KK303 pKa = 8.73VAAPDD308 pKa = 3.49GKK310 pKa = 10.42IYY312 pKa = 10.04FAHH315 pKa = 6.88KK316 pKa = 10.0GIPSGSYY323 pKa = 7.44YY324 pKa = 10.2TSLVGSIVNRR334 pKa = 11.84LRR336 pKa = 11.84IEE338 pKa = 3.82YY339 pKa = 9.97LYY341 pKa = 11.04RR342 pKa = 11.84LILGRR347 pKa = 11.84GPKK350 pKa = 9.11ICHH353 pKa = 6.11TLGDD357 pKa = 4.29DD358 pKa = 4.02SLVGDD363 pKa = 5.26DD364 pKa = 4.34EE365 pKa = 6.25LIVPSSFGLIANRR378 pKa = 11.84IGWYY382 pKa = 10.15FNIEE386 pKa = 3.65KK387 pKa = 9.58TEE389 pKa = 3.87YY390 pKa = 10.57SRR392 pKa = 11.84IPEE395 pKa = 3.96MVSFLGRR402 pKa = 11.84SYY404 pKa = 11.23KK405 pKa = 10.76GGLNVRR411 pKa = 11.84DD412 pKa = 4.13LKK414 pKa = 10.97RR415 pKa = 11.84CLRR418 pKa = 11.84LLVFPEE424 pKa = 4.14YY425 pKa = 9.57PVPSGRR431 pKa = 11.84ISAYY435 pKa = 8.48RR436 pKa = 11.84AKK438 pKa = 10.55SIAEE442 pKa = 4.03DD443 pKa = 3.49CGGISDD449 pKa = 4.83LLNRR453 pKa = 11.84VAARR457 pKa = 11.84LKK459 pKa = 10.13RR460 pKa = 11.84QYY462 pKa = 11.31GLASEE467 pKa = 4.77EE468 pKa = 3.88EE469 pKa = 4.04VPIYY473 pKa = 9.9FKK475 pKa = 10.74RR476 pKa = 11.84YY477 pKa = 9.08LPFMM481 pKa = 5.17

Molecular weight: 54.91 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

2

0

2

855

374

481

427.5

48.81

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

7.836 ± 0.625

1.17 ± 0.275

5.263 ± 0.116

5.263 ± 0.793

4.211 ± 0.212

5.497 ± 0.772

2.807 ± 0.424

7.135 ± 0.624

4.211 ± 0.635

8.304 ± 0.687

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

1.637 ± 0.487

4.444 ± 1.08

6.316 ± 1.588

3.509 ± 0.36

6.784 ± 0.402

7.251 ± 0.02

5.029 ± 0.306

5.38 ± 0.359

1.287 ± 0.032

6.667 ± 0.35
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski