Podoviridae sp. ctdb7
Average proteome isoelectric point is 6.73
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 54 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A345BQV7|A0A345BQV7_9CAUD Putative transcriptional regulator XRE family OS=Podoviridae sp. ctdb7 OX=2675443 PE=4 SV=1
MM1 pKa = 7.66 RR2 pKa = 11.84 EE3 pKa = 4.14 EE4 pKa = 4.08 KK5 pKa = 10.73 VVMYY9 pKa = 7.41 EE10 pKa = 4.04 SPEE13 pKa = 3.84 AASIQTVTGWVDD25 pKa = 2.75 PSGRR29 pKa = 11.84 FWGKK33 pKa = 9.7 DD34 pKa = 2.89 EE35 pKa = 5.23 HH36 pKa = 5.75 MARR39 pKa = 11.84 YY40 pKa = 9.42 CGSTHH45 pKa = 6.08 RR46 pKa = 11.84 QCAKK50 pKa = 10.74 NPDD53 pKa = 3.99 HH54 pKa = 6.89 PTHH57 pKa = 5.96 EE58 pKa = 4.34 TRR60 pKa = 11.84 GWCRR64 pKa = 11.84 ACQAEE69 pKa = 4.13 GDD71 pKa = 3.99 AAKK74 pKa = 9.9 FAAMPRR80 pKa = 11.84 RR81 pKa = 11.84 LWGGEE86 pKa = 4.2 PITHH90 pKa = 6.71 YY91 pKa = 11.27 DD92 pKa = 3.11 GDD94 pKa = 3.64 RR95 pKa = 11.84 YY96 pKa = 10.64 FFDD99 pKa = 4.8 EE100 pKa = 4.89 EE101 pKa = 4.96 SLLDD105 pKa = 3.93 WIVDD109 pKa = 3.78 YY110 pKa = 11.22 EE111 pKa = 4.81 IDD113 pKa = 4.28 LADD116 pKa = 4.44 LKK118 pKa = 11.39 LVFCTPNYY126 pKa = 9.41 PSQIDD131 pKa = 3.91 PSDD134 pKa = 3.88 HH135 pKa = 6.93 FSDD138 pKa = 5.22 DD139 pKa = 3.66 LPEE142 pKa = 6.28 DD143 pKa = 4.1 GEE145 pKa = 4.45 INDD148 pKa = 4.6 DD149 pKa = 3.5 QLLAAFEE156 pKa = 4.93 LLNEE160 pKa = 4.98 MISKK164 pKa = 10.26 SPPLSWSPGKK174 pKa = 10.2 EE175 pKa = 3.81 AVDD178 pKa = 4.4 LPQSFIDD185 pKa = 3.72 MVKK188 pKa = 10.35 HH189 pKa = 5.24 EE190 pKa = 5.11 RR191 pKa = 11.84 EE192 pKa = 3.99 DD193 pKa = 3.46 AAA195 pKa = 5.66
Molecular weight: 22.24 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 4.415
IPC2_protein 4.418
IPC_protein 4.38
Toseland 4.215
ProMoST 4.507
Dawson 4.342
Bjellqvist 4.495
Wikipedia 4.24
Rodwell 4.215
Grimsley 4.113
Solomon 4.342
Lehninger 4.291
Nozaki 4.457
DTASelect 4.647
Thurlkill 4.228
EMBOSS 4.253
Sillero 4.495
Patrickios 3.592
IPC_peptide 4.342
IPC2_peptide 4.482
IPC2.peptide.svr19 4.427
Protein with the highest isoelectric point:
>tr|A0A345BQT9|A0A345BQT9_9CAUD Uncharacterized protein OS=Podoviridae sp. ctdb7 OX=2675443 PE=4 SV=1
MM1 pKa = 7.13 TRR3 pKa = 11.84 IEE5 pKa = 4.52 KK6 pKa = 10.32 KK7 pKa = 9.3 LTMVVSALASSIAVAPGSSIVSMAASMAFTAIWCGVIAPYY47 pKa = 9.78 PRR49 pKa = 11.84 PGRR52 pKa = 11.84 SARR55 pKa = 11.84 PVPAGEE61 pKa = 4.09 AACGQLLVALRR72 pKa = 11.84 LDD74 pKa = 4.3 RR75 pKa = 11.84 YY76 pKa = 9.14 WRR78 pKa = 11.84 WLHH81 pKa = 5.75 LLRR84 pKa = 11.84 RR85 pKa = 11.84 RR86 pKa = 11.84 PRR88 pKa = 11.84 TSLCVWQPEE97 pKa = 4.24 PGAQEE102 pKa = 3.95 PRR104 pKa = 11.84 DD105 pKa = 3.66
Molecular weight: 11.53 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.318
IPC2_protein 9.663
IPC_protein 10.833
Toseland 10.818
ProMoST 10.921
Dawson 10.891
Bjellqvist 10.76
Wikipedia 11.228
Rodwell 10.804
Grimsley 10.95
Solomon 11.169
Lehninger 11.111
Nozaki 10.833
DTASelect 10.745
Thurlkill 10.818
EMBOSS 11.257
Sillero 10.862
Patrickios 10.599
IPC_peptide 11.169
IPC2_peptide 10.218
IPC2.peptide.svr19 8.747
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
54
0
54
12606
81
856
233.4
25.69
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
11.772 ± 0.54
1.158 ± 0.166
5.957 ± 0.205
6.275 ± 0.413
3.022 ± 0.196
8.028 ± 0.378
1.896 ± 0.203
4.419 ± 0.221
4.117 ± 0.257
8.448 ± 0.299
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.61 ± 0.153
3.538 ± 0.303
4.474 ± 0.211
5.037 ± 0.404
7.338 ± 0.365
5.902 ± 0.277
5.648 ± 0.303
6.005 ± 0.257
1.602 ± 0.154
2.753 ± 0.221
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here