Acholeplasma hippikon

Taxonomy: cellular organisms; Bacteria; Terrabacteria group; Tenericutes; Mollicutes; Acholeplasmatales; Acholeplasmataceae; Acholeplasma

Average proteome isoelectric point is 6.63

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 1335 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A449BLC8|A0A449BLC8_9MOLU Biofilm formation regulator HmsP OS=Acholeplasma hippikon OX=264636 GN=NCTC10172_01307 PE=4 SV=1
MM1 pKa = 7.67KK2 pKa = 9.93IIKK5 pKa = 9.74LAIISLMIVFCSFGIAYY22 pKa = 9.88AFTFNKK28 pKa = 10.34ADD30 pKa = 4.02DD31 pKa = 4.29LRR33 pKa = 11.84TNLAFEE39 pKa = 4.71LDD41 pKa = 3.68AYY43 pKa = 10.82AVFGDD48 pKa = 3.77VSITNITIEE57 pKa = 3.94RR58 pKa = 11.84DD59 pKa = 3.42YY60 pKa = 11.29EE61 pKa = 4.01IVEE64 pKa = 4.37DD65 pKa = 4.16VVIDD69 pKa = 4.06SDD71 pKa = 4.05LDD73 pKa = 3.78LYY75 pKa = 10.99RR76 pKa = 11.84VVIDD80 pKa = 4.7YY81 pKa = 9.34EE82 pKa = 3.96ILTDD86 pKa = 4.64KK87 pKa = 10.3EE88 pKa = 4.56FPSEE92 pKa = 4.16GIYY95 pKa = 10.42AYY97 pKa = 9.34LTASFLNEE105 pKa = 4.05EE106 pKa = 4.15VSEE109 pKa = 4.62LINVTSSEE117 pKa = 3.74MDD119 pKa = 2.86INMVNSSKK127 pKa = 10.47IVGSFEE133 pKa = 4.64VYY135 pKa = 9.49FYY137 pKa = 11.11WNIGVSTLTYY147 pKa = 9.54EE148 pKa = 4.29QYY150 pKa = 10.77QVLQSAVDD158 pKa = 3.79EE159 pKa = 4.44NGKK162 pKa = 9.25IALKK166 pKa = 10.48LQINQIIQQ174 pKa = 3.06

Molecular weight: 19.8 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A449BKQ2|A0A449BKQ2_9MOLU 50S ribosomal protein L6 OS=Acholeplasma hippikon OX=264636 GN=rplF PE=3 SV=1
MM1 pKa = 7.35KK2 pKa = 9.43RR3 pKa = 11.84TYY5 pKa = 9.95QPSKK9 pKa = 9.64IKK11 pKa = 10.09HH12 pKa = 4.75QRR14 pKa = 11.84THH16 pKa = 5.46GFRR19 pKa = 11.84ARR21 pKa = 11.84MATANGRR28 pKa = 11.84KK29 pKa = 8.93VLARR33 pKa = 11.84RR34 pKa = 11.84RR35 pKa = 11.84AKK37 pKa = 10.02GRR39 pKa = 11.84HH40 pKa = 4.51VLTVV44 pKa = 3.12

Molecular weight: 5.17 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

1335

0

1335

441607

35

6124

330.8

37.6

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

5.869 ± 0.07

0.425 ± 0.014

5.598 ± 0.05

6.876 ± 0.058

4.976 ± 0.046

5.926 ± 0.075

1.812 ± 0.031

8.796 ± 0.075

8.319 ± 0.086

9.981 ± 0.081

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.421 ± 0.034

5.79 ± 0.073

2.917 ± 0.032

3.018 ± 0.036

3.344 ± 0.044

5.836 ± 0.052

5.655 ± 0.077

6.877 ± 0.056

0.776 ± 0.023

4.788 ± 0.064
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski