Streptococcus satellite phage Javan287

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Siphoviridae; unclassified Siphoviridae

Average proteome isoelectric point is 6.6

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 13 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A4D5ZID9|A0A4D5ZID9_9VIRU Integrase/recombinase OS=Streptococcus satellite phage Javan287 OX=2558609 GN=JavanS287_0001 PE=3 SV=1
MM1 pKa = 6.97EE2 pKa = 5.67LKK4 pKa = 10.38RR5 pKa = 11.84IEE7 pKa = 4.09EE8 pKa = 4.04LGFYY12 pKa = 10.93GNFYY16 pKa = 11.02DD17 pKa = 5.93EE18 pKa = 4.86YY19 pKa = 10.82LQSDD23 pKa = 4.17YY24 pKa = 11.52HH25 pKa = 7.86NNDD28 pKa = 2.61LTEE31 pKa = 4.09QEE33 pKa = 4.47EE34 pKa = 4.45SDD36 pKa = 3.54LNYY39 pKa = 10.69YY40 pKa = 10.75GSTILSQSQKK50 pKa = 10.52GNSLGEE56 pKa = 4.09VYY58 pKa = 10.61KK59 pKa = 10.91KK60 pKa = 10.21IFLMGKK66 pKa = 8.79IAGIKK71 pKa = 7.69QEE73 pKa = 4.02RR74 pKa = 11.84SGNSS78 pKa = 2.88

Molecular weight: 9.03 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A4D5ZNE6|A0A4D5ZNE6_9VIRU Helicase loader OS=Streptococcus satellite phage Javan287 OX=2558609 GN=JavanS287_0008 PE=4 SV=1
MM1 pKa = 7.29FLKK4 pKa = 9.47TQIFFTKK11 pKa = 10.09VKK13 pKa = 10.16LFGVFTIFSYY23 pKa = 10.22IYY25 pKa = 10.12SVRR28 pKa = 11.84KK29 pKa = 7.99YY30 pKa = 9.79KK31 pKa = 10.66KK32 pKa = 10.15SIVLIYY38 pKa = 10.01EE39 pKa = 4.07

Molecular weight: 4.79 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

13

0

13

2007

39

387

154.4

17.89

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

5.531 ± 0.783

0.747 ± 0.191

5.68 ± 0.757

8.819 ± 1.244

3.538 ± 0.449

4.335 ± 0.42

1.046 ± 0.293

7.025 ± 0.44

11.659 ± 0.555

10.812 ± 0.798

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.292 ± 0.398

6.477 ± 0.4

2.242 ± 0.37

4.634 ± 0.634

3.687 ± 0.365

5.082 ± 0.561

5.63 ± 0.634

5.082 ± 0.584

0.947 ± 0.245

4.733 ± 0.4
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski