Amino acid dipepetide frequency for Lactococcus phage P008

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10-3.

For more information see sequence space article on Wikipedia.

AlaCysAspGluPheGlyHisIleLysLeuMetAsnProGlnArgSerThrValTrpTyrXaa
Ala
0.783AlaAla: 0.783 ± 0.32
0.112AlaCys: 0.112 ± 0.099
3.021AlaAsp: 3.021 ± 0.58
3.916AlaGlu: 3.916 ± 0.641
3.133AlaPhe: 3.133 ± 0.497
4.923AlaGly: 4.923 ± 0.922
0.895AlaHis: 0.895 ± 0.275
5.258AlaIle: 5.258 ± 1.32
6.265AlaLys: 6.265 ± 0.882
6.825AlaLeu: 6.825 ± 0.792
2.126AlaMet: 2.126 ± 0.665
4.252AlaAsn: 4.252 ± 0.864
0.783AlaPro: 0.783 ± 0.317
2.014AlaGln: 2.014 ± 0.412
2.014AlaArg: 2.014 ± 0.378
3.245AlaSer: 3.245 ± 0.693
2.909AlaThr: 2.909 ± 0.716
5.035AlaVal: 5.035 ± 1.119
1.902AlaTrp: 1.902 ± 0.523
1.678AlaTyr: 1.678 ± 0.409
0.0AlaXaa: 0.0 ± 0.0
Cys
0.336CysAla: 0.336 ± 0.194
0.112CysCys: 0.112 ± 0.125
0.112CysAsp: 0.112 ± 0.098
0.336CysGlu: 0.336 ± 0.211
0.0CysPhe: 0.0 ± 0.0
0.671CysGly: 0.671 ± 0.296
0.112CysHis: 0.112 ± 0.122
0.448CysIle: 0.448 ± 0.238
0.783CysLys: 0.783 ± 0.332
0.224CysLeu: 0.224 ± 0.144
0.0CysMet: 0.0 ± 0.0
0.336CysAsn: 0.336 ± 0.194
0.112CysPro: 0.112 ± 0.122
0.336CysGln: 0.336 ± 0.216
0.448CysArg: 0.448 ± 0.201
0.448CysSer: 0.448 ± 0.206
0.224CysThr: 0.224 ± 0.16
0.448CysVal: 0.448 ± 0.23
0.0CysTrp: 0.0 ± 0.0
0.336CysTyr: 0.336 ± 0.199
0.0CysXaa: 0.0 ± 0.0
Asp
2.014AspAla: 2.014 ± 0.513
0.112AspCys: 0.112 ± 0.113
3.245AspAsp: 3.245 ± 0.814
4.252AspGlu: 4.252 ± 0.639
3.692AspPhe: 3.692 ± 0.657
3.468AspGly: 3.468 ± 0.556
0.671AspHis: 0.671 ± 0.296
3.692AspIle: 3.692 ± 0.616
5.37AspLys: 5.37 ± 0.614
6.042AspLeu: 6.042 ± 0.849
1.119AspMet: 1.119 ± 0.28
3.692AspAsn: 3.692 ± 0.67
1.119AspPro: 1.119 ± 0.48
0.671AspGln: 0.671 ± 0.268
2.014AspArg: 2.014 ± 0.457
2.909AspSer: 2.909 ± 0.655
5.035AspThr: 5.035 ± 0.747
3.245AspVal: 3.245 ± 0.597
0.895AspTrp: 0.895 ± 0.267
3.356AspTyr: 3.356 ± 0.585
0.0AspXaa: 0.0 ± 0.0
Glu
3.804GluAla: 3.804 ± 0.788
0.448GluCys: 0.448 ± 0.28
3.58GluAsp: 3.58 ± 0.672
6.154GluGlu: 6.154 ± 1.016
4.252GluPhe: 4.252 ± 0.532
1.79GluGly: 1.79 ± 0.369
1.119GluHis: 1.119 ± 0.457
5.37GluIle: 5.37 ± 0.697
5.818GluLys: 5.818 ± 1.032
10.517GluLeu: 10.517 ± 1.55
2.797GluMet: 2.797 ± 0.59
5.258GluAsn: 5.258 ± 0.719
1.566GluPro: 1.566 ± 0.434
3.356GluGln: 3.356 ± 0.67
2.797GluArg: 2.797 ± 0.614
3.692GluSer: 3.692 ± 0.569
4.475GluThr: 4.475 ± 0.743
5.035GluVal: 5.035 ± 0.773
1.007GluTrp: 1.007 ± 0.324
2.909GluTyr: 2.909 ± 0.712
0.0GluXaa: 0.0 ± 0.0
Phe
3.356PheAla: 3.356 ± 0.838
0.224PheCys: 0.224 ± 0.163
3.468PheAsp: 3.468 ± 0.601
2.35PheGlu: 2.35 ± 0.573
1.902PhePhe: 1.902 ± 0.697
2.35PheGly: 2.35 ± 0.475
0.448PheHis: 0.448 ± 0.201
3.133PheIle: 3.133 ± 0.614
4.923PheLys: 4.923 ± 0.673
2.685PheLeu: 2.685 ± 0.53
0.895PheMet: 0.895 ± 0.353
2.685PheAsn: 2.685 ± 0.775
0.783PhePro: 0.783 ± 0.337
1.231PheGln: 1.231 ± 0.352
1.231PheArg: 1.231 ± 0.305
4.363PheSer: 4.363 ± 1.086
2.35PheThr: 2.35 ± 0.493
3.021PheVal: 3.021 ± 0.436
0.224PheTrp: 0.224 ± 0.149
1.678PheTyr: 1.678 ± 0.397
0.0PheXaa: 0.0 ± 0.0
Gly
3.692GlyAla: 3.692 ± 1.251
0.224GlyCys: 0.224 ± 0.154
3.245GlyAsp: 3.245 ± 0.689
3.916GlyGlu: 3.916 ± 0.642
2.685GlyPhe: 2.685 ± 0.734
4.028GlyGly: 4.028 ± 0.895
0.783GlyHis: 0.783 ± 0.254
4.252GlyIle: 4.252 ± 1.38
6.937GlyLys: 6.937 ± 0.774
5.706GlyLeu: 5.706 ± 1.038
0.783GlyMet: 0.783 ± 0.307
3.804GlyAsn: 3.804 ± 0.709
0.336GlyPro: 0.336 ± 0.191
1.902GlyGln: 1.902 ± 0.386
1.79GlyArg: 1.79 ± 0.333
4.363GlySer: 4.363 ± 0.996
3.245GlyThr: 3.245 ± 0.935
5.818GlyVal: 5.818 ± 1.156
1.119GlyTrp: 1.119 ± 0.335
3.468GlyTyr: 3.468 ± 0.647
0.0GlyXaa: 0.0 ± 0.0
His
0.671HisAla: 0.671 ± 0.228
0.224HisCys: 0.224 ± 0.162
0.783HisAsp: 0.783 ± 0.299
0.336HisGlu: 0.336 ± 0.181
0.783HisPhe: 0.783 ± 0.317
1.231HisGly: 1.231 ± 0.423
0.0HisHis: 0.0 ± 0.0
1.119HisIle: 1.119 ± 0.323
0.559HisLys: 0.559 ± 0.217
1.119HisLeu: 1.119 ± 0.39
0.224HisMet: 0.224 ± 0.206
1.678HisAsn: 1.678 ± 0.531
0.224HisPro: 0.224 ± 0.171
0.224HisGln: 0.224 ± 0.158
0.336HisArg: 0.336 ± 0.278
0.224HisSer: 0.224 ± 0.146
0.783HisThr: 0.783 ± 0.268
0.895HisVal: 0.895 ± 0.334
0.112HisTrp: 0.112 ± 0.117
0.671HisTyr: 0.671 ± 0.316
0.0HisXaa: 0.0 ± 0.0
Ile
4.923IleAla: 4.923 ± 0.731
0.224IleCys: 0.224 ± 0.135
4.811IleAsp: 4.811 ± 0.664
5.818IleGlu: 5.818 ± 0.912
2.797IlePhe: 2.797 ± 0.543
3.245IleGly: 3.245 ± 0.888
0.783IleHis: 0.783 ± 0.324
5.147IleIle: 5.147 ± 0.713
7.384IleLys: 7.384 ± 0.769
5.594IleLeu: 5.594 ± 0.974
1.678IleMet: 1.678 ± 0.437
4.811IleAsn: 4.811 ± 0.626
1.79IlePro: 1.79 ± 0.431
1.902IleGln: 1.902 ± 0.45
1.678IleArg: 1.678 ± 0.441
3.021IleSer: 3.021 ± 0.652
5.035IleThr: 5.035 ± 0.685
4.14IleVal: 4.14 ± 0.838
1.566IleTrp: 1.566 ± 0.615
2.35IleTyr: 2.35 ± 0.469
0.0IleXaa: 0.0 ± 0.0
Lys
6.937LysAla: 6.937 ± 1.041
0.783LysCys: 0.783 ± 0.45
5.147LysAsp: 5.147 ± 0.635
9.286LysGlu: 9.286 ± 1.585
2.35LysPhe: 2.35 ± 0.493
5.594LysGly: 5.594 ± 0.989
1.343LysHis: 1.343 ± 0.467
5.482LysIle: 5.482 ± 0.78
9.51LysLys: 9.51 ± 1.171
7.16LysLeu: 7.16 ± 0.766
2.909LysMet: 2.909 ± 0.392
5.482LysAsn: 5.482 ± 0.88
1.343LysPro: 1.343 ± 0.461
3.58LysGln: 3.58 ± 0.601
4.14LysArg: 4.14 ± 0.809
5.706LysSer: 5.706 ± 0.969
5.258LysThr: 5.258 ± 0.673
6.377LysVal: 6.377 ± 0.786
1.343LysTrp: 1.343 ± 0.358
3.245LysTyr: 3.245 ± 0.633
0.0LysXaa: 0.0 ± 0.0
Leu
5.258LeuAla: 5.258 ± 0.688
0.112LeuCys: 0.112 ± 0.122
5.035LeuAsp: 5.035 ± 0.727
6.154LeuGlu: 6.154 ± 0.952
3.58LeuPhe: 3.58 ± 0.734
4.14LeuGly: 4.14 ± 0.751
1.007LeuHis: 1.007 ± 0.368
6.377LeuIle: 6.377 ± 0.945
7.608LeuLys: 7.608 ± 0.846
7.272LeuLeu: 7.272 ± 1.358
2.014LeuMet: 2.014 ± 0.532
5.818LeuAsn: 5.818 ± 0.964
3.245LeuPro: 3.245 ± 0.594
3.692LeuGln: 3.692 ± 0.516
3.021LeuArg: 3.021 ± 0.59
5.706LeuSer: 5.706 ± 0.795
6.265LeuThr: 6.265 ± 0.835
6.713LeuVal: 6.713 ± 0.861
1.454LeuTrp: 1.454 ± 0.374
4.363LeuTyr: 4.363 ± 0.792
0.0LeuXaa: 0.0 ± 0.0
Met
2.126MetAla: 2.126 ± 0.541
0.112MetCys: 0.112 ± 0.096
1.454MetAsp: 1.454 ± 0.388
1.79MetGlu: 1.79 ± 0.544
0.336MetPhe: 0.336 ± 0.173
1.343MetGly: 1.343 ± 0.338
0.336MetHis: 0.336 ± 0.199
2.238MetIle: 2.238 ± 0.505
2.573MetLys: 2.573 ± 0.495
2.35MetLeu: 2.35 ± 0.59
0.336MetMet: 0.336 ± 0.197
1.902MetAsn: 1.902 ± 0.395
0.448MetPro: 0.448 ± 0.239
1.678MetGln: 1.678 ± 0.386
0.559MetArg: 0.559 ± 0.284
1.343MetSer: 1.343 ± 0.339
1.454MetThr: 1.454 ± 0.444
1.678MetVal: 1.678 ± 0.313
0.224MetTrp: 0.224 ± 0.158
1.566MetTyr: 1.566 ± 0.435
0.0MetXaa: 0.0 ± 0.0
Asn
4.699AsnAla: 4.699 ± 1.135
0.112AsnCys: 0.112 ± 0.122
3.58AsnAsp: 3.58 ± 0.672
5.594AsnGlu: 5.594 ± 0.806
2.238AsnPhe: 2.238 ± 0.648
6.042AsnGly: 6.042 ± 0.853
0.671AsnHis: 0.671 ± 0.241
3.245AsnIle: 3.245 ± 0.62
7.272AsnLys: 7.272 ± 1.333
5.706AsnLeu: 5.706 ± 0.672
1.902AsnMet: 1.902 ± 0.404
3.692AsnAsn: 3.692 ± 0.933
2.126AsnPro: 2.126 ± 0.466
1.678AsnGln: 1.678 ± 0.442
2.238AsnArg: 2.238 ± 0.469
5.035AsnSer: 5.035 ± 0.882
4.028AsnThr: 4.028 ± 0.702
3.245AsnVal: 3.245 ± 0.604
0.783AsnTrp: 0.783 ± 0.399
2.126AsnTyr: 2.126 ± 0.651
0.0AsnXaa: 0.0 ± 0.0
Pro
1.343ProAla: 1.343 ± 0.443
0.112ProCys: 0.112 ± 0.118
1.454ProAsp: 1.454 ± 0.407
1.902ProGlu: 1.902 ± 0.434
1.119ProPhe: 1.119 ± 0.388
0.336ProGly: 0.336 ± 0.183
0.224ProHis: 0.224 ± 0.143
1.566ProIle: 1.566 ± 0.5
2.014ProLys: 2.014 ± 0.523
1.678ProLeu: 1.678 ± 0.453
0.671ProMet: 0.671 ± 0.254
2.238ProAsn: 2.238 ± 0.76
0.559ProPro: 0.559 ± 0.261
0.671ProGln: 0.671 ± 0.289
0.559ProArg: 0.559 ± 0.304
1.231ProSer: 1.231 ± 0.32
2.238ProThr: 2.238 ± 0.436
2.126ProVal: 2.126 ± 0.614
0.224ProTrp: 0.224 ± 0.171
0.448ProTyr: 0.448 ± 0.205
0.0ProXaa: 0.0 ± 0.0
Gln
3.356GlnAla: 3.356 ± 0.642
0.224GlnCys: 0.224 ± 0.144
2.35GlnAsp: 2.35 ± 0.479
2.126GlnGlu: 2.126 ± 0.531
1.231GlnPhe: 1.231 ± 0.338
2.573GlnGly: 2.573 ± 0.549
0.336GlnHis: 0.336 ± 0.176
1.566GlnIle: 1.566 ± 0.354
2.573GlnLys: 2.573 ± 0.556
3.133GlnLeu: 3.133 ± 0.502
1.119GlnMet: 1.119 ± 0.303
1.902GlnAsn: 1.902 ± 0.439
1.119GlnPro: 1.119 ± 0.38
1.79GlnGln: 1.79 ± 0.41
1.678GlnArg: 1.678 ± 0.469
2.014GlnSer: 2.014 ± 0.344
2.35GlnThr: 2.35 ± 0.357
1.902GlnVal: 1.902 ± 0.463
0.559GlnTrp: 0.559 ± 0.222
1.119GlnTyr: 1.119 ± 0.339
0.0GlnXaa: 0.0 ± 0.0
Arg
2.126ArgAla: 2.126 ± 0.633
0.336ArgCys: 0.336 ± 0.162
1.343ArgAsp: 1.343 ± 0.418
2.685ArgGlu: 2.685 ± 0.554
0.783ArgPhe: 0.783 ± 0.276
2.014ArgGly: 2.014 ± 0.403
0.895ArgHis: 0.895 ± 0.344
2.126ArgIle: 2.126 ± 0.476
4.14ArgLys: 4.14 ± 0.798
3.692ArgLeu: 3.692 ± 0.659
0.671ArgMet: 0.671 ± 0.286
2.461ArgAsn: 2.461 ± 0.557
0.559ArgPro: 0.559 ± 0.248
1.454ArgGln: 1.454 ± 0.379
1.79ArgArg: 1.79 ± 0.484
2.126ArgSer: 2.126 ± 0.535
1.902ArgThr: 1.902 ± 0.434
2.014ArgVal: 2.014 ± 0.391
0.224ArgTrp: 0.224 ± 0.155
2.238ArgTyr: 2.238 ± 0.519
0.0ArgXaa: 0.0 ± 0.0
Ser
4.811SerAla: 4.811 ± 1.622
0.895SerCys: 0.895 ± 0.336
3.468SerAsp: 3.468 ± 0.698
3.692SerGlu: 3.692 ± 0.611
3.133SerPhe: 3.133 ± 0.539
6.154SerGly: 6.154 ± 1.597
0.336SerHis: 0.336 ± 0.212
4.028SerIle: 4.028 ± 0.614
4.811SerLys: 4.811 ± 0.793
4.028SerLeu: 4.028 ± 0.667
2.238SerMet: 2.238 ± 0.385
4.028SerAsn: 4.028 ± 0.779
1.566SerPro: 1.566 ± 0.576
2.238SerGln: 2.238 ± 0.453
2.238SerArg: 2.238 ± 0.36
5.035SerSer: 5.035 ± 1.025
3.021SerThr: 3.021 ± 0.638
4.587SerVal: 4.587 ± 0.869
0.671SerTrp: 0.671 ± 0.313
2.126SerTyr: 2.126 ± 0.407
0.0SerXaa: 0.0 ± 0.0
Thr
4.811ThrAla: 4.811 ± 0.718
0.336ThrCys: 0.336 ± 0.217
3.245ThrAsp: 3.245 ± 0.665
6.042ThrGlu: 6.042 ± 0.791
2.685ThrPhe: 2.685 ± 0.615
4.363ThrGly: 4.363 ± 0.634
0.224ThrHis: 0.224 ± 0.158
4.587ThrIle: 4.587 ± 0.787
4.14ThrLys: 4.14 ± 0.553
6.042ThrLeu: 6.042 ± 0.651
1.007ThrMet: 1.007 ± 0.29
4.363ThrAsn: 4.363 ± 0.86
2.014ThrPro: 2.014 ± 0.393
3.021ThrGln: 3.021 ± 0.54
1.79ThrArg: 1.79 ± 0.444
3.804ThrSer: 3.804 ± 0.62
2.909ThrThr: 2.909 ± 0.57
3.692ThrVal: 3.692 ± 0.722
0.895ThrTrp: 0.895 ± 0.305
1.902ThrTyr: 1.902 ± 0.483
0.0ThrXaa: 0.0 ± 0.0
Val
4.028ValAla: 4.028 ± 0.744
0.336ValCys: 0.336 ± 0.173
4.587ValAsp: 4.587 ± 0.813
4.587ValGlu: 4.587 ± 0.673
3.58ValPhe: 3.58 ± 0.711
4.14ValGly: 4.14 ± 0.743
0.671ValHis: 0.671 ± 0.22
5.035ValIle: 5.035 ± 0.656
7.049ValLys: 7.049 ± 0.941
3.468ValLeu: 3.468 ± 0.534
2.014ValMet: 2.014 ± 0.532
2.685ValAsn: 2.685 ± 0.431
1.902ValPro: 1.902 ± 0.546
1.454ValGln: 1.454 ± 0.422
3.468ValArg: 3.468 ± 0.795
5.594ValSer: 5.594 ± 1.205
5.035ValThr: 5.035 ± 0.886
3.245ValVal: 3.245 ± 0.707
0.671ValTrp: 0.671 ± 0.257
3.021ValTyr: 3.021 ± 0.454
0.0ValXaa: 0.0 ± 0.0
Trp
0.559TrpAla: 0.559 ± 0.192
0.224TrpCys: 0.224 ± 0.164
0.783TrpAsp: 0.783 ± 0.367
0.671TrpGlu: 0.671 ± 0.255
0.783TrpPhe: 0.783 ± 0.314
0.895TrpGly: 0.895 ± 0.382
0.336TrpHis: 0.336 ± 0.17
0.448TrpIle: 0.448 ± 0.187
0.783TrpLys: 0.783 ± 0.264
2.014TrpLeu: 2.014 ± 0.572
0.448TrpMet: 0.448 ± 0.198
1.119TrpAsn: 1.119 ± 0.31
0.0TrpPro: 0.0 ± 0.0
0.895TrpGln: 0.895 ± 0.308
0.336TrpArg: 0.336 ± 0.26
1.454TrpSer: 1.454 ± 0.376
0.783TrpThr: 0.783 ± 0.34
0.559TrpVal: 0.559 ± 0.319
0.112TrpTrp: 0.112 ± 0.112
0.895TrpTyr: 0.895 ± 0.295
0.0TrpXaa: 0.0 ± 0.0
Tyr
1.454TyrAla: 1.454 ± 0.487
0.559TyrCys: 0.559 ± 0.288
1.902TyrAsp: 1.902 ± 0.77
4.14TyrGlu: 4.14 ± 0.758
2.238TyrPhe: 2.238 ± 0.477
2.797TyrGly: 2.797 ± 0.596
1.007TyrHis: 1.007 ± 0.354
3.58TyrIle: 3.58 ± 0.823
2.461TyrLys: 2.461 ± 0.589
3.692TyrLeu: 3.692 ± 0.758
0.783TyrMet: 0.783 ± 0.323
3.804TyrAsn: 3.804 ± 0.625
1.119TyrPro: 1.119 ± 0.375
1.231TyrGln: 1.231 ± 0.487
1.454TyrArg: 1.454 ± 0.44
1.79TyrSer: 1.79 ± 0.414
2.573TyrThr: 2.573 ± 0.612
2.797TyrVal: 2.797 ± 0.64
0.112TyrTrp: 0.112 ± 0.104
2.35TyrTyr: 2.35 ± 0.549
0.0TyrXaa: 0.0 ± 0.0
Xaa
0.0XaaAla: 0.0 ± 0.0
0.0XaaCys: 0.0 ± 0.0
0.0XaaAsp: 0.0 ± 0.0
0.0XaaGlu: 0.0 ± 0.0
0.0XaaPhe: 0.0 ± 0.0
0.0XaaGly: 0.0 ± 0.0
0.0XaaHis: 0.0 ± 0.0
0.0XaaIle: 0.0 ± 0.0
0.0XaaLys: 0.0 ± 0.0
0.0XaaLeu: 0.0 ± 0.0
0.0XaaMet: 0.0 ± 0.0
0.0XaaAsn: 0.0 ± 0.0
0.0XaaPro: 0.0 ± 0.0
0.0XaaGln: 0.0 ± 0.0
0.0XaaArg: 0.0 ± 0.0
0.0XaaSer: 0.0 ± 0.0
0.0XaaThr: 0.0 ± 0.0
0.0XaaVal: 0.0 ± 0.0
0.0XaaTrp: 0.0 ± 0.0
0.0XaaTyr: 0.0 ± 0.0
0.0XaaXaa: 0.0 ± 0.0
Statistics based on 58 proteins (8939 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski