Helicobacter phage KHP40
Average proteome isoelectric point is 7.49
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 32 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|I7H0J2|I7H0J2_9CAUD Uncharacterized protein OS=Helicobacter phage KHP40 OX=1204178 PE=4 SV=1
MM1 pKa = 7.58 KK2 pKa = 9.99 FLEE5 pKa = 4.11 MLAISVLIFILGISFILAVYY25 pKa = 9.0 FSVEE29 pKa = 3.91 ALCYY33 pKa = 10.24 GG34 pKa = 3.7
Molecular weight: 3.82 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 4.782
IPC2_protein 5.194
IPC_protein 4.431
Toseland 4.342
ProMoST 4.558
Dawson 4.355
Bjellqvist 4.533
Wikipedia 4.253
Rodwell 4.304
Grimsley 4.279
Solomon 4.317
Lehninger 4.266
Nozaki 4.533
DTASelect 4.444
Thurlkill 4.393
EMBOSS 4.253
Sillero 4.533
Patrickios 0.299
IPC_peptide 4.329
IPC2_peptide 4.533
IPC2.peptide.svr19 4.491
Protein with the highest isoelectric point:
>tr|I7GUT4|I7GUT4_9CAUD ddrB-ParB domain-containing protein OS=Helicobacter phage KHP40 OX=1204178 PE=4 SV=1
MM1 pKa = 7.87 RR2 pKa = 11.84 NYY4 pKa = 10.46 KK5 pKa = 9.79 HH6 pKa = 6.59 ANKK9 pKa = 9.99 NFKK12 pKa = 10.04 PKK14 pKa = 9.66 VWTNSVLLEE23 pKa = 3.99 NGILEE28 pKa = 4.07 ILEE31 pKa = 3.99 ALKK34 pKa = 9.99 TIEE37 pKa = 4.05 NRR39 pKa = 11.84 SRR41 pKa = 11.84 SQVLEE46 pKa = 3.58 RR47 pKa = 11.84 LIIFFIEE54 pKa = 4.3 TQKK57 pKa = 10.96 GQSNEE62 pKa = 4.23 MAWKK66 pKa = 10.1 RR67 pKa = 11.84 SQRR70 pKa = 11.84 AYY72 pKa = 10.19 KK73 pKa = 9.34 RR74 pKa = 11.84 TLTNKK79 pKa = 8.22 TEE81 pKa = 4.07 KK82 pKa = 10.72 NKK84 pKa = 10.46 LKK86 pKa = 10.44 RR87 pKa = 11.84 KK88 pKa = 7.37 QFEE91 pKa = 4.52 RR92 pKa = 11.84 IEE94 pKa = 4.02 KK95 pKa = 7.2 TQKK98 pKa = 10.45 KK99 pKa = 9.41 KK100 pKa = 10.55 QLQAGANYY108 pKa = 8.81 PFSFFEE114 pKa = 4.23 RR115 pKa = 11.84 AA116 pKa = 3.06
Molecular weight: 13.95 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.203
IPC2_protein 9.706
IPC_protein 9.867
Toseland 10.789
ProMoST 10.277
Dawson 10.847
Bjellqvist 10.452
Wikipedia 10.965
Rodwell 11.418
Grimsley 10.877
Solomon 10.906
Lehninger 10.891
Nozaki 10.745
DTASelect 10.452
Thurlkill 10.76
EMBOSS 11.155
Sillero 10.76
Patrickios 11.14
IPC_peptide 10.921
IPC2_peptide 8.858
IPC2.peptide.svr19 8.763
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
32
0
32
8251
34
1725
257.8
29.61
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
6.799 ± 0.373
0.788 ± 0.254
5.308 ± 0.381
8.205 ± 0.389
4.715 ± 0.287
4.048 ± 0.26
1.454 ± 0.164
6.92 ± 0.282
10.896 ± 0.443
10.496 ± 0.301
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
1.769 ± 0.197
8.193 ± 0.443
2.485 ± 0.176
4.545 ± 0.338
4.072 ± 0.266
6.472 ± 0.322
4.569 ± 0.244
4.084 ± 0.432
0.545 ± 0.115
3.636 ± 0.244
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here