Amino acid dipepetide frequency for [Clostridium] spiroforme DSM 1552

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10^-3.

For more information see sequence space article on Wikipedia.

	Ala	Cys	Asp	Glu	Phe	Gly	His	Ile	Lys	Leu	Met	Asn	Pro	Gln	Arg	Ser	Thr	Val	Trp	Tyr	Xaa
Ala	3.041AlaAla: 3.041 ± 0.11	0.799AlaCys: 0.799 ± 0.036	2.542AlaAsp: 2.542 ± 0.071	2.081AlaGlu: 2.081 ± 0.074	2.596AlaPhe: 2.596 ± 0.065	3.642AlaGly: 3.642 ± 0.085	0.866AlaHis: 0.866 ± 0.038	5.865AlaIle: 5.865 ± 0.099	5.294AlaLys: 5.294 ± 0.103	5.983AlaLeu: 5.983 ± 0.096	1.792AlaMet: 1.792 ± 0.047	3.527AlaAsn: 3.527 ± 0.086	1.4AlaPro: 1.4 ± 0.049	1.294AlaGln: 1.294 ± 0.046	1.941AlaArg: 1.941 ± 0.059	3.822AlaSer: 3.822 ± 0.081	3.389AlaThr: 3.389 ± 0.085	3.522AlaVal: 3.522 ± 0.086	0.368AlaTrp: 0.368 ± 0.029	2.573AlaTyr: 2.573 ± 0.069	0.0AlaXaa: 0.0 ± 0.0
Cys	0.613CysAla: 0.613 ± 0.032	0.222CysCys: 0.222 ± 0.02	0.836CysAsp: 0.836 ± 0.038	0.52CysGlu: 0.52 ± 0.03	0.661CysPhe: 0.661 ± 0.03	0.998CysGly: 0.998 ± 0.05	0.31CysHis: 0.31 ± 0.02	1.22CysIle: 1.22 ± 0.043	1.149CysLys: 1.149 ± 0.049	1.353CysLeu: 1.353 ± 0.045	0.305CysMet: 0.305 ± 0.019	0.755CysAsn: 0.755 ± 0.035	0.481CysPro: 0.481 ± 0.03	0.401CysGln: 0.401 ± 0.025	0.366CysArg: 0.366 ± 0.021	0.795CysSer: 0.795 ± 0.037	0.559CysThr: 0.559 ± 0.03	0.799CysVal: 0.799 ± 0.035	0.085CysTrp: 0.085 ± 0.011	0.577CysTyr: 0.577 ± 0.03	0.0CysXaa: 0.0 ± 0.0
Asp	3.195AspAla: 3.195 ± 0.083	0.75AspCys: 0.75 ± 0.03	3.946AspAsp: 3.946 ± 0.096	5.252AspGlu: 5.252 ± 0.088	2.893AspPhe: 2.893 ± 0.07	3.851AspGly: 3.851 ± 0.109	0.943AspHis: 0.943 ± 0.038	5.65AspIle: 5.65 ± 0.097	5.224AspLys: 5.224 ± 0.096	5.661AspLeu: 5.661 ± 0.103	1.513AspMet: 1.513 ± 0.042	3.932AspAsn: 3.932 ± 0.084	1.515AspPro: 1.515 ± 0.041	1.745AspGln: 1.745 ± 0.048	1.772AspArg: 1.772 ± 0.051	3.344AspSer: 3.344 ± 0.078	2.813AspThr: 2.813 ± 0.077	4.034AspVal: 4.034 ± 0.08	0.387AspTrp: 0.387 ± 0.029	3.54AspTyr: 3.54 ± 0.074	0.0AspXaa: 0.0 ± 0.0
Glu	4.209GluAla: 4.209 ± 0.09	0.688GluCys: 0.688 ± 0.033	3.583GluAsp: 3.583 ± 0.081	4.763GluGlu: 4.763 ± 0.116	2.964GluPhe: 2.964 ± 0.065	3.292GluGly: 3.292 ± 0.082	1.043GluHis: 1.043 ± 0.042	6.772GluIle: 6.772 ± 0.114	5.744GluLys: 5.744 ± 0.101	6.434GluLeu: 6.434 ± 0.11	2.005GluMet: 2.005 ± 0.059	4.883GluAsn: 4.883 ± 0.087	1.343GluPro: 1.343 ± 0.047	2.099GluGln: 2.099 ± 0.057	2.078GluArg: 2.078 ± 0.067	2.991GluSer: 2.991 ± 0.067	3.273GluThr: 3.273 ± 0.075	4.617GluVal: 4.617 ± 0.083	0.439GluTrp: 0.439 ± 0.027	3.299GluTyr: 3.299 ± 0.078	0.0GluXaa: 0.0 ± 0.0
Phe	2.26PheAla: 2.26 ± 0.056	0.562PheCys: 0.562 ± 0.029	3.306PheAsp: 3.306 ± 0.064	2.988PheGlu: 2.988 ± 0.061	1.752PhePhe: 1.752 ± 0.064	2.514PheGly: 2.514 ± 0.059	0.637PheHis: 0.637 ± 0.028	4.472PheIle: 4.472 ± 0.109	3.529PheLys: 3.529 ± 0.072	3.474PheLeu: 3.474 ± 0.092	1.118PheMet: 1.118 ± 0.041	2.961PheAsn: 2.961 ± 0.073	1.073PhePro: 1.073 ± 0.037	1.003PheGln: 1.003 ± 0.039	1.117PheArg: 1.117 ± 0.035	2.956PheSer: 2.956 ± 0.073	2.11PheThr: 2.11 ± 0.057	2.889PheVal: 2.889 ± 0.065	0.281PheTrp: 0.281 ± 0.023	1.962PheTyr: 1.962 ± 0.051	0.0PheXaa: 0.0 ± 0.0
Gly	3.481GlyAla: 3.481 ± 0.095	0.931GlyCys: 0.931 ± 0.041	3.329GlyAsp: 3.329 ± 0.081	3.216GlyGlu: 3.216 ± 0.08	2.789GlyPhe: 2.789 ± 0.073	3.652GlyGly: 3.652 ± 0.086	1.156GlyHis: 1.156 ± 0.045	6.08GlyIle: 6.08 ± 0.109	5.092GlyLys: 5.092 ± 0.096	5.231GlyLeu: 5.231 ± 0.085	1.701GlyMet: 1.701 ± 0.055	3.299GlyAsn: 3.299 ± 0.077	1.069GlyPro: 1.069 ± 0.044	1.625GlyGln: 1.625 ± 0.055	1.839GlyArg: 1.839 ± 0.059	3.386GlySer: 3.386 ± 0.075	3.288GlyThr: 3.288 ± 0.076	3.967GlyVal: 3.967 ± 0.069	0.489GlyTrp: 0.489 ± 0.039	3.242GlyTyr: 3.242 ± 0.073	0.0GlyXaa: 0.0 ± 0.0
His	0.816HisAla: 0.816 ± 0.035	0.274HisCys: 0.274 ± 0.02	1.14HisAsp: 1.14 ± 0.042	1.048HisGlu: 1.048 ± 0.043	0.764HisPhe: 0.764 ± 0.039	1.146HisGly: 1.146 ± 0.043	0.5HisHis: 0.5 ± 0.03	1.382HisIle: 1.382 ± 0.041	1.076HisLys: 1.076 ± 0.041	1.54HisLeu: 1.54 ± 0.051	0.432HisMet: 0.432 ± 0.019	0.896HisAsn: 0.896 ± 0.04	0.689HisPro: 0.689 ± 0.03	0.715HisGln: 0.715 ± 0.031	0.639HisArg: 0.639 ± 0.028	0.992HisSer: 0.992 ± 0.037	0.76HisThr: 0.76 ± 0.034	0.939HisVal: 0.939 ± 0.035	0.129HisTrp: 0.129 ± 0.015	0.792HisTyr: 0.792 ± 0.034	0.0HisXaa: 0.0 ± 0.0
Ile	5.495IleAla: 5.495 ± 0.098	1.445IleCys: 1.445 ± 0.051	6.557IleAsp: 6.557 ± 0.104	6.779IleGlu: 6.779 ± 0.098	3.886IlePhe: 3.886 ± 0.096	5.442IleGly: 5.442 ± 0.108	1.452IleHis: 1.452 ± 0.048	9.371IleIle: 9.371 ± 0.208	8.256IleLys: 8.256 ± 0.125	8.217IleLeu: 8.217 ± 0.155	2.346IleMet: 2.346 ± 0.061	6.479IleAsn: 6.479 ± 0.104	2.912IlePro: 2.912 ± 0.07	2.323IleGln: 2.323 ± 0.065	2.795IleArg: 2.795 ± 0.066	6.217IleSer: 6.217 ± 0.116	4.838IleThr: 4.838 ± 0.094	6.177IleVal: 6.177 ± 0.108	0.502IleTrp: 0.502 ± 0.03	3.886IleTyr: 3.886 ± 0.082	0.0IleXaa: 0.0 ± 0.0
Lys	4.849LysAla: 4.849 ± 0.098	0.924LysCys: 0.924 ± 0.043	5.552LysAsp: 5.552 ± 0.092	7.773LysGlu: 7.773 ± 0.111	2.468LysPhe: 2.468 ± 0.065	3.932LysGly: 3.932 ± 0.085	1.423LysHis: 1.423 ± 0.046	8.041LysIle: 8.041 ± 0.117	7.854LysLys: 7.854 ± 0.141	7.103LysLeu: 7.103 ± 0.105	2.664LysMet: 2.664 ± 0.059	5.572LysAsn: 5.572 ± 0.091	1.996LysPro: 1.996 ± 0.049	3.192LysGln: 3.192 ± 0.078	3.137LysArg: 3.137 ± 0.067	4.018LysSer: 4.018 ± 0.081	4.424LysThr: 4.424 ± 0.073	5.203LysVal: 5.203 ± 0.086	0.618LysTrp: 0.618 ± 0.034	4.37LysTyr: 4.37 ± 0.089	0.0LysXaa: 0.0 ± 0.0
Leu	5.535LeuAla: 5.535 ± 0.102	1.2LeuCys: 1.2 ± 0.045	6.024LeuAsp: 6.024 ± 0.106	6.298LeuGlu: 6.298 ± 0.101	3.934LeuPhe: 3.934 ± 0.097	5.379LeuGly: 5.379 ± 0.094	1.272LeuHis: 1.272 ± 0.045	8.504LeuIle: 8.504 ± 0.151	8.654LeuLys: 8.654 ± 0.134	8.4LeuLeu: 8.4 ± 0.125	2.384LeuMet: 2.384 ± 0.062	6.093LeuAsn: 6.093 ± 0.093	2.757LeuPro: 2.757 ± 0.07	2.594LeuGln: 2.594 ± 0.059	2.705LeuArg: 2.705 ± 0.066	6.117LeuSer: 6.117 ± 0.099	4.804LeuThr: 4.804 ± 0.084	5.765LeuVal: 5.765 ± 0.087	0.506LeuTrp: 0.506 ± 0.026	3.551LeuTyr: 3.551 ± 0.069	0.0LeuXaa: 0.0 ± 0.0
Met	1.78MetAla: 1.78 ± 0.062	0.278MetCys: 0.278 ± 0.019	1.462MetAsp: 1.462 ± 0.053	1.826MetGlu: 1.826 ± 0.056	1.181MetPhe: 1.181 ± 0.048	1.402MetGly: 1.402 ± 0.048	0.416MetHis: 0.416 ± 0.021	2.678MetIle: 2.678 ± 0.07	2.583MetLys: 2.583 ± 0.06	2.507MetLeu: 2.507 ± 0.059	0.935MetMet: 0.935 ± 0.036	1.727MetAsn: 1.727 ± 0.045	0.802MetPro: 0.802 ± 0.034	0.947MetGln: 0.947 ± 0.034	0.724MetArg: 0.724 ± 0.037	1.705MetSer: 1.705 ± 0.051	1.298MetThr: 1.298 ± 0.045	1.554MetVal: 1.554 ± 0.047	0.158MetTrp: 0.158 ± 0.015	1.02MetTyr: 1.02 ± 0.036	0.0MetXaa: 0.0 ± 0.0
Asn	3.491AsnAla: 3.491 ± 0.103	0.819AsnCys: 0.819 ± 0.037	4.322AsnAsp: 4.322 ± 0.097	4.602AsnGlu: 4.602 ± 0.08	2.402AsnPhe: 2.402 ± 0.066	3.835AsnGly: 3.835 ± 0.081	1.17AsnHis: 1.17 ± 0.041	5.882AsnIle: 5.882 ± 0.111	5.467AsnLys: 5.467 ± 0.105	5.405AsnLeu: 5.405 ± 0.107	1.574AsnMet: 1.574 ± 0.046	4.701AsnAsn: 4.701 ± 0.107	2.025AsnPro: 2.025 ± 0.058	2.564AsnGln: 2.564 ± 0.062	2.173AsnArg: 2.173 ± 0.068	3.432AsnSer: 3.432 ± 0.077	2.91AsnThr: 2.91 ± 0.075	4.004AsnVal: 4.004 ± 0.081	0.47AsnTrp: 0.47 ± 0.027	3.438AsnTyr: 3.438 ± 0.072	0.0AsnXaa: 0.0 ± 0.0
Pro	1.325ProAla: 1.325 ± 0.047	0.348ProCys: 0.348 ± 0.021	1.597ProAsp: 1.597 ± 0.049	2.025ProGlu: 2.025 ± 0.064	1.358ProPhe: 1.358 ± 0.046	1.636ProGly: 1.636 ± 0.054	0.461ProHis: 0.461 ± 0.025	2.457ProIle: 2.457 ± 0.061	1.906ProLys: 1.906 ± 0.052	2.387ProLeu: 2.387 ± 0.059	0.697ProMet: 0.697 ± 0.031	1.655ProAsn: 1.655 ± 0.051	0.436ProPro: 0.436 ± 0.025	0.82ProGln: 0.82 ± 0.032	0.825ProArg: 0.825 ± 0.037	1.681ProSer: 1.681 ± 0.049	1.608ProThr: 1.608 ± 0.056	1.991ProVal: 1.991 ± 0.059	0.232ProTrp: 0.232 ± 0.018	1.357ProTyr: 1.357 ± 0.043	0.0ProXaa: 0.0 ± 0.0
Gln	1.996GlnAla: 1.996 ± 0.058	0.274GlnCys: 0.274 ± 0.021	1.765GlnAsp: 1.765 ± 0.05	2.426GlnGlu: 2.426 ± 0.066	1.145GlnPhe: 1.145 ± 0.037	1.701GlnGly: 1.701 ± 0.046	0.453GlnHis: 0.453 ± 0.027	2.889GlnIle: 2.889 ± 0.071	2.451GlnLys: 2.451 ± 0.06	2.947GlnLeu: 2.947 ± 0.066	0.893GlnMet: 0.893 ± 0.038	1.758GlnAsn: 1.758 ± 0.054	0.717GlnPro: 0.717 ± 0.032	1.037GlnGln: 1.037 ± 0.042	1.15GlnArg: 1.15 ± 0.044	1.576GlnSer: 1.576 ± 0.051	1.561GlnThr: 1.561 ± 0.05	1.951GlnVal: 1.951 ± 0.054	0.252GlnTrp: 0.252 ± 0.022	1.442GlnTyr: 1.442 ± 0.049	0.0GlnXaa: 0.0 ± 0.0
Arg	1.536ArgAla: 1.536 ± 0.053	0.443ArgCys: 0.443 ± 0.027	1.733ArgAsp: 1.733 ± 0.051	2.033ArgGlu: 2.033 ± 0.052	1.512ArgPhe: 1.512 ± 0.044	1.758ArgGly: 1.758 ± 0.048	0.577ArgHis: 0.577 ± 0.033	2.957ArgIle: 2.957 ± 0.068	2.9ArgLys: 2.9 ± 0.067	3.176ArgLeu: 3.176 ± 0.072	0.967ArgMet: 0.967 ± 0.033	1.877ArgAsn: 1.877 ± 0.052	0.904ArgPro: 0.904 ± 0.038	1.03ArgGln: 1.03 ± 0.038	1.301ArgArg: 1.301 ± 0.047	1.656ArgSer: 1.656 ± 0.05	1.388ArgThr: 1.388 ± 0.047	2.043ArgVal: 2.043 ± 0.064	0.224ArgTrp: 0.224 ± 0.019	1.835ArgTyr: 1.835 ± 0.053	0.0ArgXaa: 0.0 ± 0.0
Ser	3.072SerAla: 3.072 ± 0.078	0.711SerCys: 0.711 ± 0.035	3.517SerAsp: 3.517 ± 0.076	3.172SerGlu: 3.172 ± 0.066	3.039SerPhe: 3.039 ± 0.07	4.026SerGly: 4.026 ± 0.074	0.977SerHis: 0.977 ± 0.037	5.465SerIle: 5.465 ± 0.097	5.239SerLys: 5.239 ± 0.086	5.928SerLeu: 5.928 ± 0.092	1.519SerMet: 1.519 ± 0.046	3.896SerAsn: 3.896 ± 0.084	1.404SerPro: 1.404 ± 0.044	1.804SerGln: 1.804 ± 0.063	1.973SerArg: 1.973 ± 0.059	3.944SerSer: 3.944 ± 0.089	2.896SerThr: 2.896 ± 0.079	3.554SerVal: 3.554 ± 0.075	0.47SerTrp: 0.47 ± 0.03	2.802SerTyr: 2.802 ± 0.064	0.0SerXaa: 0.0 ± 0.0
Thr	2.93ThrAla: 2.93 ± 0.074	0.535ThrCys: 0.535 ± 0.029	2.638ThrAsp: 2.638 ± 0.076	2.144ThrGlu: 2.144 ± 0.07	2.243ThrPhe: 2.243 ± 0.064	3.537ThrGly: 3.537 ± 0.078	0.799ThrHis: 0.799 ± 0.036	5.048ThrIle: 5.048 ± 0.096	4.132ThrLys: 4.132 ± 0.087	5.041ThrLeu: 5.041 ± 0.103	1.308ThrMet: 1.308 ± 0.045	3.199ThrAsn: 3.199 ± 0.083	1.86ThrPro: 1.86 ± 0.055	1.267ThrGln: 1.267 ± 0.051	1.516ThrArg: 1.516 ± 0.044	3.237ThrSer: 3.237 ± 0.071	3.23ThrThr: 3.23 ± 0.097	3.719ThrVal: 3.719 ± 0.073	0.436ThrTrp: 0.436 ± 0.027	2.492ThrTyr: 2.492 ± 0.072	0.0ThrXaa: 0.0 ± 0.0
Val	3.927ValAla: 3.927 ± 0.091	0.991ValCys: 0.991 ± 0.041	4.303ValAsp: 4.303 ± 0.087	4.466ValGlu: 4.466 ± 0.08	2.782ValPhe: 2.782 ± 0.072	3.867ValGly: 3.867 ± 0.073	0.94ValHis: 0.94 ± 0.04	6.208ValIle: 6.208 ± 0.095	4.89ValLys: 4.89 ± 0.085	5.998ValLeu: 5.998 ± 0.09	1.6ValMet: 1.6 ± 0.055	4.029ValAsn: 4.029 ± 0.098	1.793ValPro: 1.793 ± 0.057	1.354ValGln: 1.354 ± 0.046	1.801ValArg: 1.801 ± 0.06	4.328ValSer: 4.328 ± 0.08	3.436ValThr: 3.436 ± 0.088	4.607ValVal: 4.607 ± 0.096	0.354ValTrp: 0.354 ± 0.026	2.886ValTyr: 2.886 ± 0.06	0.0ValXaa: 0.0 ± 0.0
Trp	0.358TrpAla: 0.358 ± 0.027	0.116TrpCys: 0.116 ± 0.014	0.387TrpAsp: 0.387 ± 0.025	0.296TrpGlu: 0.296 ± 0.023	0.328TrpPhe: 0.328 ± 0.022	0.436TrpGly: 0.436 ± 0.027	0.169TrpHis: 0.169 ± 0.016	0.595TrpIle: 0.595 ± 0.033	0.471TrpLys: 0.471 ± 0.027	0.795TrpLeu: 0.795 ± 0.037	0.201TrpMet: 0.201 ± 0.019	0.421TrpAsn: 0.421 ± 0.031	0.154TrpPro: 0.154 ± 0.016	0.341TrpGln: 0.341 ± 0.022	0.194TrpArg: 0.194 ± 0.015	0.395TrpSer: 0.395 ± 0.025	0.345TrpThr: 0.345 ± 0.023	0.379TrpVal: 0.379 ± 0.026	0.085TrpTrp: 0.085 ± 0.01	0.362TrpTyr: 0.362 ± 0.025	0.0TrpXaa: 0.0 ± 0.0
Tyr	2.427TyrAla: 2.427 ± 0.065	0.695TyrCys: 0.695 ± 0.03	3.421TyrAsp: 3.421 ± 0.081	2.81TyrGlu: 2.81 ± 0.064	2.265TyrPhe: 2.265 ± 0.064	2.817TyrGly: 2.817 ± 0.072	1.105TyrHis: 1.105 ± 0.04	3.84TyrIle: 3.84 ± 0.07	3.202TyrLys: 3.202 ± 0.068	4.897TyrLeu: 4.897 ± 0.097	1.029TyrMet: 1.029 ± 0.036	3.025TyrAsn: 3.025 ± 0.072	1.473TyrPro: 1.473 ± 0.042	2.225TyrGln: 2.225 ± 0.062	1.803TyrArg: 1.803 ± 0.055	2.817TyrSer: 2.817 ± 0.066	2.38TyrThr: 2.38 ± 0.063	2.847TyrVal: 2.847 ± 0.064	0.348TyrTrp: 0.348 ± 0.023	2.485TyrTyr: 2.485 ± 0.072	0.0TyrXaa: 0.0 ± 0.0
Xaa	0.0XaaAla: 0.0 ± 0.0	0.0XaaCys: 0.0 ± 0.0	0.0XaaAsp: 0.0 ± 0.0	0.0XaaGlu: 0.0 ± 0.0	0.0XaaPhe: 0.0 ± 0.0	0.0XaaGly: 0.0 ± 0.0	0.0XaaHis: 0.0 ± 0.0	0.0XaaIle: 0.0 ± 0.0	0.0XaaLys: 0.0 ± 0.0	0.0XaaLeu: 0.0 ± 0.0	0.0XaaMet: 0.0 ± 0.0	0.0XaaAsn: 0.0 ± 0.0	0.0XaaPro: 0.0 ± 0.0	0.0XaaGln: 0.0 ± 0.0	0.0XaaArg: 0.0 ± 0.0	0.0XaaSer: 0.0 ± 0.0	0.0XaaThr: 0.0 ± 0.0	0.0XaaVal: 0.0 ± 0.0	0.0XaaTrp: 0.0 ± 0.0	0.0XaaTyr: 0.0 ± 0.0	0.0XaaXaa: 0.0 ± 0.0

Statistics based on 2358 proteins (715586 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944

Contact: Lukasz P. Kozlowski

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