Rhizophagus irregularis (strain DAOM 197198w) (Glomus intraradices)
Average proteome isoelectric point is 6.56
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 28669 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A015KZ70|A0A015KZ70_RHIIW Uncharacterized protein OS=Rhizophagus irregularis (strain DAOM 197198w) OX=1432141 GN=RirG_065160 PE=4 SV=1
MM1 pKa = 7.55 EE2 pKa = 6.14 FIKK5 pKa = 10.7 VDD7 pKa = 3.58 PRR9 pKa = 11.84 KK10 pKa = 10.08 VLYY13 pKa = 9.42 SQYY16 pKa = 10.6 SIRR19 pKa = 11.84 PRR21 pKa = 11.84 FRR23 pKa = 11.84 DD24 pKa = 3.45 DD25 pKa = 3.91 EE26 pKa = 4.98 LIEE29 pKa = 4.1 DD30 pKa = 4.78 TIDD33 pKa = 3.35 QLVSGEE39 pKa = 4.13 ISPKK43 pKa = 9.65 NIEE46 pKa = 4.54 HH47 pKa = 6.85 IRR49 pKa = 11.84 VCTFKK54 pKa = 11.33 GKK56 pKa = 7.98 MHH58 pKa = 6.55 SLDD61 pKa = 3.23 NRR63 pKa = 11.84 RR64 pKa = 11.84 LYY66 pKa = 11.08 AFRR69 pKa = 11.84 EE70 pKa = 4.52 AIKK73 pKa = 10.15 RR74 pKa = 11.84 GCSFRR79 pKa = 11.84 TVPAIRR85 pKa = 11.84 SYY87 pKa = 11.42 EE88 pKa = 3.85 FDD90 pKa = 3.17 EE91 pKa = 5.32 LKK93 pKa = 10.78 RR94 pKa = 11.84 KK95 pKa = 6.99 MTSPPSPDD103 pKa = 3.02 YY104 pKa = 11.3 SVIKK108 pKa = 10.11 VRR110 pKa = 11.84 RR111 pKa = 11.84 DD112 pKa = 2.84 IYY114 pKa = 10.74 IPEE117 pKa = 4.67 DD118 pKa = 4.26 LYY120 pKa = 11.62 DD121 pKa = 6.09 DD122 pKa = 6.17 DD123 pKa = 7.62 DD124 pKa = 7.22 DD125 pKa = 7.42 DD126 pKa = 6.53 DD127 pKa = 6.57 DD128 pKa = 5.33 EE129 pKa = 5.33 YY130 pKa = 11.63 EE131 pKa = 4.36 EE132 pKa = 5.05 YY133 pKa = 10.81 DD134 pKa = 5.04 DD135 pKa = 6.24 EE136 pKa = 6.17 EE137 pKa = 6.95 DD138 pKa = 3.79 DD139 pKa = 5.8 DD140 pKa = 6.08 EE141 pKa = 6.04 YY142 pKa = 11.76 DD143 pKa = 3.69 EE144 pKa = 4.54 YY145 pKa = 11.67 DD146 pKa = 3.36 EE147 pKa = 5.51 YY148 pKa = 11.51 EE149 pKa = 4.7 EE150 pKa = 5.12 EE151 pKa = 6.17 DD152 pKa = 3.84 EE153 pKa = 6.24 DD154 pKa = 6.69 DD155 pKa = 4.39 DD156 pKa = 6.47 DD157 pKa = 5.92 EE158 pKa = 6.75 YY159 pKa = 11.75 DD160 pKa = 4.41 DD161 pKa = 6.03 EE162 pKa = 6.17 SDD164 pKa = 3.78 EE165 pKa = 6.34 DD166 pKa = 3.7 EE167 pKa = 5.21 DD168 pKa = 5.28 EE169 pKa = 4.44 YY170 pKa = 11.81 DD171 pKa = 3.51 EE172 pKa = 5.31 EE173 pKa = 5.42 YY174 pKa = 11.1 YY175 pKa = 10.95 GYY177 pKa = 10.73 DD178 pKa = 4.15 YY179 pKa = 11.52 DD180 pKa = 6.45 DD181 pKa = 5.21 EE182 pKa = 7.57 DD183 pKa = 6.09 DD184 pKa = 5.89 DD185 pKa = 6.09 EE186 pKa = 6.77 YY187 pKa = 11.77 DD188 pKa = 3.56 DD189 pKa = 6.13 DD190 pKa = 6.17 EE191 pKa = 6.07 YY192 pKa = 11.78 DD193 pKa = 3.02 EE194 pKa = 5.48 YY195 pKa = 11.92 YY196 pKa = 10.73 DD197 pKa = 4.2 YY198 pKa = 11.71 DD199 pKa = 3.73 VLNSFGRR206 pKa = 11.84 LYY208 pKa = 10.53 II209 pKa = 4.05
Molecular weight: 25.6 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.687
IPC2_protein 3.706
IPC_protein 3.745
Toseland 3.516
ProMoST 3.897
Dawson 3.732
Bjellqvist 3.884
Wikipedia 3.656
Rodwell 3.567
Grimsley 3.427
Solomon 3.732
Lehninger 3.681
Nozaki 3.834
DTASelect 4.088
Thurlkill 3.567
EMBOSS 3.668
Sillero 3.859
Patrickios 1.38
IPC_peptide 3.719
IPC2_peptide 3.834
IPC2.peptide.svr19 3.764
Protein with the highest isoelectric point:
>tr|A0A015IVX4|A0A015IVX4_RHIIW Gag-pol fusion protein OS=Rhizophagus irregularis (strain DAOM 197198w) OX=1432141 GN=RirG_197930 PE=4 SV=1
MM1 pKa = 7.83 RR2 pKa = 11.84 NHH4 pKa = 5.99 NVINVKK10 pKa = 10.05 RR11 pKa = 11.84 MRR13 pKa = 11.84 NHH15 pKa = 5.69 NVINVKK21 pKa = 10.05 RR22 pKa = 11.84 MRR24 pKa = 11.84 NHH26 pKa = 5.69 NVINVKK32 pKa = 10.08 RR33 pKa = 11.84 MRR35 pKa = 11.84 NHH37 pKa = 6.59 NIINVKK43 pKa = 9.76 RR44 pKa = 11.84 LRR46 pKa = 11.84 NHH48 pKa = 6.62 NIINVKK54 pKa = 9.76 RR55 pKa = 11.84 LRR57 pKa = 11.84 NHH59 pKa = 6.62 NIINVKK65 pKa = 9.76 RR66 pKa = 11.84 LRR68 pKa = 11.84 NHH70 pKa = 6.62 NIINVKK76 pKa = 9.76 RR77 pKa = 11.84 LRR79 pKa = 11.84 NHH81 pKa = 6.62 NIINVKK87 pKa = 9.76 RR88 pKa = 11.84 LRR90 pKa = 11.84 NHH92 pKa = 6.62 NIINVKK98 pKa = 9.76 RR99 pKa = 11.84 LRR101 pKa = 11.84 NHH103 pKa = 6.62 NIINVKK109 pKa = 9.76 RR110 pKa = 11.84 LRR112 pKa = 11.84 NHH114 pKa = 6.59 NIINEE119 pKa = 4.19 RR120 pKa = 11.84 PP121 pKa = 3.26
Molecular weight: 14.99 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.523
IPC2_protein 11.242
IPC_protein 12.837
Toseland 13.013
ProMoST 13.495
Dawson 13.013
Bjellqvist 12.998
Wikipedia 13.481
Rodwell 12.676
Grimsley 13.042
Solomon 13.495
Lehninger 13.408
Nozaki 13.013
DTASelect 12.998
Thurlkill 13.013
EMBOSS 13.51
Sillero 13.013
Patrickios 12.384
IPC_peptide 13.51
IPC2_peptide 12.486
IPC2.peptide.svr19 9.179
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
26712
1957
28669
10097224
49
5493
352.2
40.52
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
4.494 ± 0.016
1.585 ± 0.007
5.837 ± 0.012
6.997 ± 0.017
4.602 ± 0.01
4.486 ± 0.013
2.172 ± 0.007
8.169 ± 0.018
8.031 ± 0.019
9.01 ± 0.017
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
1.957 ± 0.006
7.378 ± 0.018
3.979 ± 0.016
3.832 ± 0.009
4.262 ± 0.013
7.783 ± 0.017
5.191 ± 0.012
4.779 ± 0.011
1.234 ± 0.005
4.217 ± 0.012
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here