Avian orthoavulavirus 1
Average proteome isoelectric point is 6.72
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 6 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|Q9WMH9|Q9WMH9_9MONO Nucleocapsid OS=Avian orthoavulavirus 1 OX=2560319 GN=NP PE=3 SV=1
MM1 pKa = 7.45 SSVFDD6 pKa = 4.24 EE7 pKa = 4.58 YY8 pKa = 11.32 EE9 pKa = 3.81 QLLAAQTRR17 pKa = 11.84 PNGAHH22 pKa = 6.5 GGGEE26 pKa = 3.98 KK27 pKa = 10.82 GSTLKK32 pKa = 10.74 VDD34 pKa = 3.72 VPVFTLNSDD43 pKa = 3.68 DD44 pKa = 5.54 PEE46 pKa = 4.53 DD47 pKa = 3.22 RR48 pKa = 11.84 WSFVVFCLRR57 pKa = 11.84 IAVSEE62 pKa = 4.28 DD63 pKa = 3.01 ANKK66 pKa = 10.05 PLRR69 pKa = 11.84 QGALISLLCSHH80 pKa = 6.17 SQVMRR85 pKa = 11.84 NHH87 pKa = 5.78 VALAGKK93 pKa = 9.58 QNEE96 pKa = 4.04 ATLAVLEE103 pKa = 4.36 IDD105 pKa = 4.13 GFANGTPQFNNRR117 pKa = 11.84 SGVSEE122 pKa = 3.88 EE123 pKa = 3.83 RR124 pKa = 11.84 AQRR127 pKa = 11.84 FAMIAGSLPRR137 pKa = 11.84 ACSNGTPFVTAGAEE151 pKa = 3.83 DD152 pKa = 4.74 DD153 pKa = 4.08 APEE156 pKa = 5.68 DD157 pKa = 3.31 ITDD160 pKa = 3.59 TLEE163 pKa = 4.86 RR164 pKa = 11.84 ILSIQAQVWVTVAKK178 pKa = 10.9 AMTAYY183 pKa = 7.35 EE184 pKa = 4.12 TADD187 pKa = 3.26 EE188 pKa = 4.57 SEE190 pKa = 3.98 TRR192 pKa = 11.84 RR193 pKa = 11.84 INKK196 pKa = 9.01 YY197 pKa = 6.52 MQQGRR202 pKa = 11.84 VQKK205 pKa = 10.55 KK206 pKa = 7.89 YY207 pKa = 10.65 ILYY210 pKa = 7.91 PVCRR214 pKa = 11.84 STIQLTIRR222 pKa = 11.84 QSLAVRR228 pKa = 11.84 IFLVSEE234 pKa = 4.38 LKK236 pKa = 10.46 RR237 pKa = 11.84 GRR239 pKa = 11.84 NTAGGTSTYY248 pKa = 10.11 YY249 pKa = 11.25 NLVGDD254 pKa = 3.76 VDD256 pKa = 4.37 SYY258 pKa = 11.6 IRR260 pKa = 11.84 NTGLTAFFLTLKK272 pKa = 10.69 YY273 pKa = 10.63 GINTKK278 pKa = 9.69 TSALALSSLSGDD290 pKa = 3.33 IQKK293 pKa = 9.34 MKK295 pKa = 10.85 QLMRR299 pKa = 11.84 LYY301 pKa = 10.82 RR302 pKa = 11.84 MKK304 pKa = 10.88 GDD306 pKa = 3.4 NAPYY310 pKa = 7.51 MTLLGDD316 pKa = 3.71 SDD318 pKa = 3.87 QMSFAPAEE326 pKa = 4.05 YY327 pKa = 9.98 AQLYY331 pKa = 8.71 SFAMGMASVLDD342 pKa = 4.0 KK343 pKa = 11.15 GTGKK347 pKa = 10.27 YY348 pKa = 8.45 QFARR352 pKa = 11.84 DD353 pKa = 3.88 FMSTSFWRR361 pKa = 11.84 LGVEE365 pKa = 4.05 YY366 pKa = 10.28 AQAQGSSINEE376 pKa = 3.69 DD377 pKa = 2.85 MAAEE381 pKa = 4.45 LKK383 pKa = 8.96 LTPAARR389 pKa = 11.84 RR390 pKa = 11.84 GLAAAAQRR398 pKa = 11.84 VSEE401 pKa = 4.31 EE402 pKa = 4.1 TSSIDD407 pKa = 3.75 MPTQQVGVLTGLSEE421 pKa = 4.57 GGSQALQGGSNRR433 pKa = 11.84 SQGQPEE439 pKa = 4.24 AGDD442 pKa = 4.17 GEE444 pKa = 4.82 TQFLDD449 pKa = 4.29 LMRR452 pKa = 11.84 AVANSMRR459 pKa = 11.84 EE460 pKa = 4.25 APNSAQGTPQSGPPPTPGPSQDD482 pKa = 4.47 NDD484 pKa = 3.98 TDD486 pKa = 2.94 WGYY489 pKa = 11.97
Molecular weight: 53.08 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 5.172
IPC2_protein 5.27
IPC_protein 5.207
Toseland 5.105
ProMoST 5.334
Dawson 5.181
Bjellqvist 5.308
Wikipedia 5.054
Rodwell 5.092
Grimsley 5.029
Solomon 5.181
Lehninger 5.13
Nozaki 5.296
DTASelect 5.461
Thurlkill 5.118
EMBOSS 5.092
Sillero 5.359
Patrickios 4.202
IPC_peptide 5.181
IPC2_peptide 5.359
IPC2.peptide.svr19 5.379
Protein with the highest isoelectric point:
>tr|Q9WMH9|Q9WMH9_9MONO Nucleocapsid OS=Avian orthoavulavirus 1 OX=2560319 GN=NP PE=3 SV=1
MM1 pKa = 7.99 DD2 pKa = 4.33 SSRR5 pKa = 11.84 TIGLYY10 pKa = 10.09 FDD12 pKa = 4.63 SAHH15 pKa = 6.53 SSSNLLAFPIVLQDD29 pKa = 3.17 TGDD32 pKa = 3.69 GKK34 pKa = 11.01 KK35 pKa = 10.16 QIAPQYY41 pKa = 9.71 RR42 pKa = 11.84 IQRR45 pKa = 11.84 LDD47 pKa = 2.92 LWTDD51 pKa = 3.2 SKK53 pKa = 11.18 EE54 pKa = 3.97 DD55 pKa = 3.45 SVFITTYY62 pKa = 10.97 GFIFQVGNEE71 pKa = 3.93 EE72 pKa = 4.25 ATVGMIDD79 pKa = 4.45 DD80 pKa = 3.8 KK81 pKa = 11.16 PKK83 pKa = 10.91 RR84 pKa = 11.84 EE85 pKa = 4.12 LLSAAMLCLGSVPNTGDD102 pKa = 3.72 LIEE105 pKa = 4.64 LARR108 pKa = 11.84 ACLTMIVTCKK118 pKa = 10.24 KK119 pKa = 10.24 SATNTEE125 pKa = 3.74 RR126 pKa = 11.84 MVFSVVQAPQVLQSCRR142 pKa = 11.84 VVANKK147 pKa = 10.08 YY148 pKa = 10.08 SSVNAVKK155 pKa = 10.22 HH156 pKa = 4.84 VKK158 pKa = 10.26 APEE161 pKa = 4.53 KK162 pKa = 10.52 IPGSGTLEE170 pKa = 3.93 YY171 pKa = 10.56 KK172 pKa = 10.96 VNFVSLTVVPKK183 pKa = 10.37 KK184 pKa = 10.45 DD185 pKa = 3.43 VYY187 pKa = 10.74 KK188 pKa = 10.75 IPAAVLKK195 pKa = 10.94 VSGSSLYY202 pKa = 10.92 NLALNVTINVEE213 pKa = 3.83 VDD215 pKa = 3.04 PRR217 pKa = 11.84 SPLVKK222 pKa = 10.22 SLSKK226 pKa = 10.64 SDD228 pKa = 3.09 SGYY231 pKa = 9.64 YY232 pKa = 10.76 ANLFLHH238 pKa = 6.98 IGLMTTVDD246 pKa = 3.37 RR247 pKa = 11.84 KK248 pKa = 10.11 GKK250 pKa = 10.33 KK251 pKa = 7.01 VTFDD255 pKa = 3.32 KK256 pKa = 11.21 LEE258 pKa = 4.05 KK259 pKa = 10.22 KK260 pKa = 9.79 IRR262 pKa = 11.84 SLDD265 pKa = 3.67 LSVGLSDD272 pKa = 3.83 VLGPSVLVKK281 pKa = 10.85 ARR283 pKa = 11.84 GARR286 pKa = 11.84 TKK288 pKa = 10.73 LLAPFFSSSGTACYY302 pKa = 9.57 PIANASPQVAKK313 pKa = 10.63 ILWSQTACLRR323 pKa = 11.84 SVKK326 pKa = 10.15 IIIQAGTQRR335 pKa = 11.84 AVAVTADD342 pKa = 3.79 HH343 pKa = 6.67 EE344 pKa = 4.77 VTSTKK349 pKa = 10.49 LEE351 pKa = 4.33 KK352 pKa = 10.74 GHH354 pKa = 6.49 TLAKK358 pKa = 10.37 YY359 pKa = 10.7 NPFKK363 pKa = 10.84 KK364 pKa = 10.49
Molecular weight: 39.6 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.063
IPC2_protein 8.887
IPC_protein 8.756
Toseland 9.633
ProMoST 9.341
Dawson 9.853
Bjellqvist 9.545
Wikipedia 9.984
Rodwell 10.335
Grimsley 9.911
Solomon 9.882
Lehninger 9.853
Nozaki 9.736
DTASelect 9.502
Thurlkill 9.706
EMBOSS 10.043
Sillero 9.794
Patrickios 7.6
IPC_peptide 9.882
IPC2_peptide 8.112
IPC2.peptide.svr19 8.022
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
6
0
6
4582
364
2204
763.7
84.29
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
7.333 ± 0.795
1.702 ± 0.254
5.085 ± 0.323
4.823 ± 0.507
3.208 ± 0.588
5.936 ± 0.774
2.008 ± 0.521
6.111 ± 0.72
4.932 ± 0.488
10.585 ± 1.059
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.597 ± 0.239
4.736 ± 0.433
4.714 ± 0.58
4.278 ± 0.848
4.998 ± 0.495
9.101 ± 0.521
7.093 ± 0.626
6.678 ± 0.457
0.764 ± 0.132
3.317 ± 0.407
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here