Wuhan insect virus 26
Average proteome isoelectric point is 5.96
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 2 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A1L3KFB4|A0A1L3KFB4_9VIRU Uncharacterized protein OS=Wuhan insect virus 26 OX=1923730 PE=4 SV=1
MM1 pKa = 7.16 LASWLSARR9 pKa = 11.84 LYY11 pKa = 10.67 RR12 pKa = 11.84 DD13 pKa = 3.35 NKK15 pKa = 10.08 DD16 pKa = 2.64 TDD18 pKa = 3.51 NILRR22 pKa = 11.84 VKK24 pKa = 9.53 TSPYY28 pKa = 9.72 SDD30 pKa = 3.27 SHH32 pKa = 6.63 VICPLDD38 pKa = 4.2 RR39 pKa = 11.84 EE40 pKa = 4.69 AEE42 pKa = 4.14 DD43 pKa = 4.64 HH44 pKa = 5.66 IHH46 pKa = 6.62 EE47 pKa = 5.1 IEE49 pKa = 4.87 LGPPVDD55 pKa = 3.57 QEE57 pKa = 4.85 LINGATWMLRR67 pKa = 11.84 AKK69 pKa = 10.42 DD70 pKa = 4.09 SYY72 pKa = 10.09 WNRR75 pKa = 11.84 PYY77 pKa = 10.96 VLHH80 pKa = 7.13 INGATTGQVEE90 pKa = 5.34 FYY92 pKa = 10.19 LSHH95 pKa = 6.0 VLGRR99 pKa = 11.84 KK100 pKa = 8.93 RR101 pKa = 11.84 RR102 pKa = 11.84 SALNYY107 pKa = 9.55 DD108 pKa = 3.85 VEE110 pKa = 4.59 IEE112 pKa = 4.16 GLNSRR117 pKa = 11.84 EE118 pKa = 4.08 LYY120 pKa = 10.8 LDD122 pKa = 3.19 IVNGDD127 pKa = 3.2 GSEE130 pKa = 4.08 PGWFHH135 pKa = 7.07 EE136 pKa = 5.02 IPWTSPDD143 pKa = 4.04 ILWSWTLDD151 pKa = 3.55 YY152 pKa = 11.6 VKK154 pKa = 10.8 LNRR157 pKa = 11.84 LEE159 pKa = 3.98 QAFAAAFEE167 pKa = 4.24 TLAAVAIQPQWSSLEE182 pKa = 3.73 ACAWQSSQIVVSFGAFSPTRR202 pKa = 11.84 ARR204 pKa = 11.84 IRR206 pKa = 11.84 SNLTGTPYY214 pKa = 10.68 TPFAEE219 pKa = 4.76 ASSFMLDD226 pKa = 3.13 EE227 pKa = 4.58 AAAPTHH233 pKa = 6.27 YY234 pKa = 8.98 FTAAAIANYY243 pKa = 8.54 YY244 pKa = 7.88 MWYY247 pKa = 10.64 GLYY250 pKa = 9.81 TLLHH254 pKa = 5.95 NEE256 pKa = 3.32 ARR258 pKa = 11.84 TRR260 pKa = 11.84 NSWKK264 pKa = 9.13 TVFSSVADD272 pKa = 4.11 EE273 pKa = 5.11 LKK275 pKa = 10.28 MLYY278 pKa = 9.64 TPMMRR283 pKa = 11.84 AACVSVITGRR293 pKa = 11.84 EE294 pKa = 3.72 YY295 pKa = 11.02 ASCMNHH301 pKa = 5.87 GAGMYY306 pKa = 9.71 IDD308 pKa = 4.04 MSSMYY313 pKa = 8.24 KK314 pKa = 9.62 QKK316 pKa = 11.07 YY317 pKa = 8.71 IGPLVPLDD325 pKa = 3.72 GTVGTSVEE333 pKa = 4.02 IEE335 pKa = 4.34 AIYY338 pKa = 10.82 APVSGGLVLGTMTGEE353 pKa = 4.51 FEE355 pKa = 4.41 TTAHH359 pKa = 5.67 LTGVRR364 pKa = 11.84 ALEE367 pKa = 4.32 CKK369 pKa = 10.44 GVVGKK374 pKa = 10.61 AFDD377 pKa = 4.01 LKK379 pKa = 10.53 EE380 pKa = 4.13 LSVLATMYY388 pKa = 10.96 RR389 pKa = 11.84 LFGYY393 pKa = 9.53 EE394 pKa = 5.33 LEE396 pKa = 4.56 LKK398 pKa = 10.25 DD399 pKa = 4.04 YY400 pKa = 8.69 QTGIIKK406 pKa = 8.52 RR407 pKa = 11.84 TWAPVRR413 pKa = 11.84 DD414 pKa = 4.22 CVVDD418 pKa = 3.58 PATIEE423 pKa = 3.91 FDD425 pKa = 4.51 PISPRR430 pKa = 11.84 LWMIEE435 pKa = 3.77 GAQPRR440 pKa = 11.84 EE441 pKa = 4.26 GRR443 pKa = 11.84 RR444 pKa = 11.84 HH445 pKa = 7.18 IIPTPQNLLSGEE457 pKa = 4.14 PVVIVHH463 pKa = 6.17 QKK465 pKa = 8.11 PTISVTKK472 pKa = 8.89 WKK474 pKa = 10.76 SRR476 pKa = 11.84 TKK478 pKa = 10.21 PPRR481 pKa = 11.84 PFIATSRR488 pKa = 11.84 VKK490 pKa = 10.96 APVQFLIKK498 pKa = 10.58 APFEE502 pKa = 4.11 YY503 pKa = 10.91 NPISFEE509 pKa = 4.04 AKK511 pKa = 8.98 EE512 pKa = 4.27 VPSHH516 pKa = 6.4 KK517 pKa = 10.33 FKK519 pKa = 11.26 LSDD522 pKa = 3.58 FQEE525 pKa = 4.98 GKK527 pKa = 9.88 SQVPPKK533 pKa = 10.33 KK534 pKa = 10.22 PEE536 pKa = 3.78 GQKK539 pKa = 9.95 ILNQRR544 pKa = 11.84 TAEE547 pKa = 4.04 PARR550 pKa = 11.84 EE551 pKa = 4.01 EE552 pKa = 4.08 EE553 pKa = 4.6 SIPTAGAAASLEE565 pKa = 4.28 SSLQQQ570 pKa = 3.47
Molecular weight: 63.92 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 5.838
IPC2_protein 5.842
IPC_protein 5.893
Toseland 6.287
ProMoST 6.224
Dawson 6.148
Bjellqvist 6.16
Wikipedia 6.16
Rodwell 6.135
Grimsley 6.44
Solomon 6.16
Lehninger 6.148
Nozaki 6.415
DTASelect 6.605
Thurlkill 6.62
EMBOSS 6.576
Sillero 6.517
Patrickios 4.101
IPC_peptide 6.173
IPC2_peptide 6.491
IPC2.peptide.svr19 6.482
Protein with the highest isoelectric point:
>tr|A0A1L3KFB4|A0A1L3KFB4_9VIRU Uncharacterized protein OS=Wuhan insect virus 26 OX=1923730 PE=4 SV=1
MM1 pKa = 7.34 QEE3 pKa = 4.09 RR4 pKa = 11.84 DD5 pKa = 3.46 SFTALDD11 pKa = 3.35 ICDD14 pKa = 3.35 GMRR17 pKa = 11.84 EE18 pKa = 4.25 GRR20 pKa = 11.84 EE21 pKa = 3.51 HH22 pKa = 5.93 TFAVRR27 pKa = 11.84 SGHH30 pKa = 5.73 VPTGLRR36 pKa = 11.84 ITCNGTYY43 pKa = 10.21 LGVPFKK49 pKa = 10.56 QAEE52 pKa = 4.21 LLLIDD57 pKa = 4.57 RR58 pKa = 11.84 LPAKK62 pKa = 10.39 LHH64 pKa = 5.7 EE65 pKa = 5.19 CNVAYY70 pKa = 10.06 DD71 pKa = 3.63 YY72 pKa = 11.65 YY73 pKa = 9.94 GTVVSARR80 pKa = 11.84 LVPGRR85 pKa = 11.84 DD86 pKa = 3.03 ATYY89 pKa = 9.5 VYY91 pKa = 10.72 YY92 pKa = 10.88 YY93 pKa = 10.38 VDD95 pKa = 3.48 QDD97 pKa = 3.76 IQPVTQNLLGMLSRR111 pKa = 11.84 HH112 pKa = 5.56 FLGDD116 pKa = 3.14 FSGYY120 pKa = 9.9 YY121 pKa = 10.14 NDD123 pKa = 3.8 WCSLDD128 pKa = 3.39 NVFYY132 pKa = 11.11 SLSKK136 pKa = 10.45 CDD138 pKa = 3.82 NPVKK142 pKa = 10.39 RR143 pKa = 11.84 HH144 pKa = 5.12 TIKK147 pKa = 10.22 TIRR150 pKa = 11.84 DD151 pKa = 3.42 LPKK154 pKa = 9.78 PKK156 pKa = 9.84 ISGAHH161 pKa = 6.42 HH162 pKa = 5.9 IHH164 pKa = 5.41 YY165 pKa = 7.07 TASEE169 pKa = 4.26 VWSVLDD175 pKa = 5.52 DD176 pKa = 3.95 RR177 pKa = 11.84 GKK179 pKa = 10.59 DD180 pKa = 3.43 AARR183 pKa = 11.84 HH184 pKa = 5.05 ALRR187 pKa = 11.84 LPADD191 pKa = 3.54 ATTSFVGGVMLWLASLPTEE210 pKa = 4.15 LLNPIIEE217 pKa = 4.53 SDD219 pKa = 3.79 LLDD222 pKa = 4.6 AEE224 pKa = 4.45 DD225 pKa = 4.19 TIEE228 pKa = 4.36 FGKK231 pKa = 10.36 KK232 pKa = 9.3 AKK234 pKa = 9.99 KK235 pKa = 10.42 LSVTAKK241 pKa = 9.18 SFQNIVEE248 pKa = 4.23 ADD250 pKa = 3.44 LRR252 pKa = 11.84 PIFEE256 pKa = 5.0 ADD258 pKa = 3.32 VLVNRR263 pKa = 11.84 DD264 pKa = 3.22 VGEE267 pKa = 4.06 VDD269 pKa = 2.96 WAGEE273 pKa = 4.0 KK274 pKa = 10.67 NNRR277 pKa = 11.84 CKK279 pKa = 10.63 PNLASVSPGEE289 pKa = 4.34 VYY291 pKa = 10.91 ARR293 pKa = 11.84 TLALFTKK300 pKa = 10.34 EE301 pKa = 4.44 DD302 pKa = 3.95 DD303 pKa = 3.92 EE304 pKa = 4.6 KK305 pKa = 11.4 ALPRR309 pKa = 11.84 RR310 pKa = 11.84 LEE312 pKa = 3.92 WEE314 pKa = 4.38 KK315 pKa = 10.87 FWDD318 pKa = 5.53 ARR320 pKa = 11.84 WQWSASGSIHH330 pKa = 6.31 SQYY333 pKa = 10.91 QEE335 pKa = 4.93 DD336 pKa = 4.84 LDD338 pKa = 4.13 SLPKK342 pKa = 10.32 EE343 pKa = 3.91 RR344 pKa = 11.84 EE345 pKa = 4.05 LKK347 pKa = 10.82 NKK349 pKa = 10.1 FIALSMQGDD358 pKa = 3.82 MPFEE362 pKa = 4.47 HH363 pKa = 6.8 FLNRR367 pKa = 11.84 KK368 pKa = 8.12 PEE370 pKa = 3.8 IVAWSSVKK378 pKa = 10.74 YY379 pKa = 9.63 EE380 pKa = 3.89 WGKK383 pKa = 9.08 MRR385 pKa = 11.84 AIYY388 pKa = 8.58 GTDD391 pKa = 2.91 LTSYY395 pKa = 10.79 VLAHH399 pKa = 5.71 YY400 pKa = 10.98 AFFNCEE406 pKa = 3.81 DD407 pKa = 4.08 TLPTDD412 pKa = 4.19 FPVGEE417 pKa = 4.21 KK418 pKa = 10.28 ARR420 pKa = 11.84 PSFVSARR427 pKa = 11.84 VAAILEE433 pKa = 4.51 GTVPLCVDD441 pKa = 4.59 FEE443 pKa = 4.98 DD444 pKa = 5.9 FNSQHH449 pKa = 6.83 SNDD452 pKa = 3.2 AMEE455 pKa = 5.1 AVVQAYY461 pKa = 9.25 IDD463 pKa = 4.37 AYY465 pKa = 8.43 SQYY468 pKa = 7.75 MTPEE472 pKa = 4.02 QVQAAMWTRR481 pKa = 11.84 EE482 pKa = 4.39 SISNTRR488 pKa = 11.84 VIDD491 pKa = 3.62 NMGTKK496 pKa = 9.69 TSYY499 pKa = 8.55 KK500 pKa = 9.92 TKK502 pKa = 8.99 GTLMSGWRR510 pKa = 11.84 LTTFINSVLNYY521 pKa = 9.94 VYY523 pKa = 9.67 TQALISGSHH532 pKa = 5.12 AMSRR536 pKa = 11.84 SVHH539 pKa = 6.05 NGDD542 pKa = 5.05 DD543 pKa = 3.48 VLLGIRR549 pKa = 11.84 NFKK552 pKa = 9.78 IVRR555 pKa = 11.84 DD556 pKa = 3.79 IVARR560 pKa = 11.84 ADD562 pKa = 3.4 KK563 pKa = 11.23 YY564 pKa = 10.77 NVRR567 pKa = 11.84 LQRR570 pKa = 11.84 TKK572 pKa = 10.81 CAFGGLAEE580 pKa = 4.41 FLRR583 pKa = 11.84 VDD585 pKa = 4.33 HH586 pKa = 6.34 MRR588 pKa = 11.84 GDD590 pKa = 3.57 YY591 pKa = 10.53 GQYY594 pKa = 8.72 LTRR597 pKa = 11.84 NIATVMHH604 pKa = 6.26 SRR606 pKa = 11.84 IEE608 pKa = 4.14 SKK610 pKa = 10.49 IAVSAVDD617 pKa = 3.43 VVQAMEE623 pKa = 4.21 DD624 pKa = 3.19 RR625 pKa = 11.84 LRR627 pKa = 11.84 EE628 pKa = 3.81 YY629 pKa = 10.48 LQRR632 pKa = 11.84 GGVWDD637 pKa = 3.61 YY638 pKa = 11.72 AVQLRR643 pKa = 11.84 EE644 pKa = 3.98 LYY646 pKa = 9.17 YY647 pKa = 11.19 DD648 pKa = 3.88 RR649 pKa = 11.84 MAPIYY654 pKa = 9.65 SLRR657 pKa = 11.84 PQDD660 pKa = 4.35 LYY662 pKa = 11.27 DD663 pKa = 3.14 IRR665 pKa = 11.84 TAHH668 pKa = 6.22 RR669 pKa = 11.84 VVGGIAEE676 pKa = 4.24 DD677 pKa = 3.54 VSARR681 pKa = 11.84 VDD683 pKa = 3.23 KK684 pKa = 10.52 FIRR687 pKa = 11.84 TSSEE691 pKa = 4.14 SIEE694 pKa = 4.27 TTLPDD699 pKa = 3.74 TLPGVDD705 pKa = 3.73 AYY707 pKa = 10.94 ARR709 pKa = 11.84 LLKK712 pKa = 10.36 KK713 pKa = 10.55 VLEE716 pKa = 4.35 LEE718 pKa = 4.49 VPVSVVSTRR727 pKa = 11.84 IRR729 pKa = 11.84 NATLNAVQLTRR740 pKa = 11.84 KK741 pKa = 7.21 TVTVEE746 pKa = 3.42 KK747 pKa = 9.4 TLNVQRR753 pKa = 11.84 YY754 pKa = 5.43 MVYY757 pKa = 9.35 RR758 pKa = 11.84 ALYY761 pKa = 9.0 RR762 pKa = 11.84 AYY764 pKa = 10.58 ADD766 pKa = 3.4 VTNNPMFGKK775 pKa = 10.58 AMLTGFIFDD784 pKa = 4.31 VLTQNAQLSAVAQLVSSAQNPMEE807 pKa = 4.14 FLRR810 pKa = 11.84 VISS813 pKa = 3.81
Molecular weight: 92.12 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 6.072
IPC2_protein 6.059
IPC_protein 6.148
Toseland 6.491
ProMoST 6.504
Dawson 6.453
Bjellqvist 6.44
Wikipedia 6.465
Rodwell 6.44
Grimsley 6.634
Solomon 6.453
Lehninger 6.453
Nozaki 6.722
DTASelect 6.912
Thurlkill 6.927
EMBOSS 6.898
Sillero 6.839
Patrickios 4.177
IPC_peptide 6.465
IPC2_peptide 6.722
IPC2.peptide.svr19 6.725
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
2
0
2
1383
570
813
691.5
78.02
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
8.749 ± 0.19
1.085 ± 0.016
5.712 ± 0.854
6.146 ± 0.533
3.615 ± 0.054
5.423 ± 0.187
2.242 ± 0.02
5.061 ± 0.371
4.7 ± 0.019
8.894 ± 0.241
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.531 ± 0.038
3.543 ± 0.285
4.917 ± 0.891
3.398 ± 0.033
6.363 ± 0.471
7.158 ± 0.286
6.291 ± 0.102
7.737 ± 0.723
1.952 ± 0.257
4.483 ± 0.139
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here