Fusarium venenatum
Average proteome isoelectric point is 6.37
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 13945 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A2L2T2E5|A0A2L2T2E5_9HYPO Uncharacterized protein OS=Fusarium venenatum OX=56646 PE=4 SV=1
MM1 pKa = 7.47 KK2 pKa = 10.58 AFIFIAALLTAAEE15 pKa = 4.18 AVLAKK20 pKa = 10.71 DD21 pKa = 3.99 LVAVEE26 pKa = 4.9 APADD30 pKa = 3.82 TWCITYY36 pKa = 10.58 LSTYY40 pKa = 9.64 LATVTNPGDD49 pKa = 3.9 ILPSDD54 pKa = 3.58 ASGNDD59 pKa = 3.58 GPDD62 pKa = 3.23 AQNSGRR68 pKa = 11.84 LPFAPSLHH76 pKa = 5.5 PTFRR80 pKa = 11.84 RR81 pKa = 11.84 NTSISVTRR89 pKa = 11.84 SAMSTDD95 pKa = 3.15 VRR97 pKa = 11.84 DD98 pKa = 3.74 TLGPEE103 pKa = 4.14 SSLSTSVVINTVDD116 pKa = 3.36 TSPVNSEE123 pKa = 4.13 SSDD126 pKa = 3.81 LDD128 pKa = 3.47 VDD130 pKa = 3.85 PTAAQSSYY138 pKa = 10.89 GVSTITDD145 pKa = 3.86 GLSTMAASTVDD156 pKa = 3.65 AEE158 pKa = 4.5 ATSTSTDD165 pKa = 3.07 IIEE168 pKa = 4.13 PAGRR172 pKa = 11.84 IVIFLIQTTDD182 pKa = 2.99 NEE184 pKa = 3.57 KK185 pKa = 9.17 RR186 pKa = 11.84 AIYY189 pKa = 9.96 RR190 pKa = 11.84 RR191 pKa = 11.84 VISGFIGLNNPSVCTFAATFNLAEE215 pKa = 4.45 GQLFADD221 pKa = 5.2 GVPIYY226 pKa = 11.02 YY227 pKa = 10.32 SGEE230 pKa = 4.04 DD231 pKa = 3.68 YY232 pKa = 11.25 KK233 pKa = 11.19 EE234 pKa = 4.47 LSAQGRR240 pKa = 11.84 PPSGSITSGFADD252 pKa = 3.35 SGGTLTFRR260 pKa = 11.84 NSDD263 pKa = 3.98 LPNGEE268 pKa = 5.23 AGFCQDD274 pKa = 4.7 ADD276 pKa = 3.72 GQVYY280 pKa = 7.83 ITFTTGPSGCVPVNLAAYY298 pKa = 9.83 NVEE301 pKa = 3.9 QCQDD305 pKa = 2.84 GRR307 pKa = 11.84 LIGLDD312 pKa = 3.56 EE313 pKa = 4.4 LTSSVSGTAITTADD327 pKa = 3.43 QSFSAPSTFGEE338 pKa = 4.47 LPEE341 pKa = 4.4 STDD344 pKa = 4.45 LIQSQSIIPSSQTSEE359 pKa = 4.46 FEE361 pKa = 3.94 ASATSLSHH369 pKa = 4.91 VTEE372 pKa = 4.67 SSTQEE377 pKa = 3.67 LGGASSQEE385 pKa = 3.97 TSALTVRR392 pKa = 11.84 EE393 pKa = 4.0 DD394 pKa = 3.96 LEE396 pKa = 4.52 TLSSVLTTSGDD407 pKa = 3.8 SASEE411 pKa = 4.07 PTSASTPHH419 pKa = 6.48 LSSEE423 pKa = 4.29 TSDD426 pKa = 4.82 LLDD429 pKa = 3.6 STTTSSDD436 pKa = 2.92 GTEE439 pKa = 3.99 LSTTSGVEE447 pKa = 4.04 SQTTTGIEE455 pKa = 4.02 TSHH458 pKa = 7.85 IEE460 pKa = 4.17 TTIKK464 pKa = 10.09 TSVSEE469 pKa = 3.98 TTIILEE475 pKa = 4.34 TTEE478 pKa = 4.03 ATPTEE483 pKa = 4.56 GDD485 pKa = 3.34 TSSTEE490 pKa = 4.0 VVSDD494 pKa = 3.47 VDD496 pKa = 3.64 TTAATDD502 pKa = 3.68 TTDD505 pKa = 3.26 TTTTNADD512 pKa = 3.69 VITDD516 pKa = 4.44 LEE518 pKa = 4.83 DD519 pKa = 3.46 STTMEE524 pKa = 4.63 AEE526 pKa = 4.01 TTAAEE531 pKa = 4.48 TTTSVKK537 pKa = 9.31 PTTTTEE543 pKa = 4.05 TATEE547 pKa = 4.31 PEE549 pKa = 4.42 DD550 pKa = 3.59 LTTTEE555 pKa = 5.2 AEE557 pKa = 4.32 TTTPEE562 pKa = 3.61 ITTTAEE568 pKa = 3.83 LTTTTEE574 pKa = 4.22 AAPEE578 pKa = 4.06 PAPQFACGDD587 pKa = 3.71 PGYY590 pKa = 7.01 TTTYY594 pKa = 9.01 TYY596 pKa = 11.62 NSVTFNLQCEE606 pKa = 4.51 SNYY609 pKa = 10.71 SYY611 pKa = 11.24 FGLEE615 pKa = 4.18 TFSTASFGEE624 pKa = 4.33 CLSRR628 pKa = 11.84 CALNSACNGIVWRR641 pKa = 11.84 RR642 pKa = 11.84 ASLLCSLTTGVTAYY656 pKa = 10.53 GPDD659 pKa = 3.12 NRR661 pKa = 11.84 FDD663 pKa = 3.61 VATVASRR670 pKa = 11.84 AA671 pKa = 3.56
Molecular weight: 69.93 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.705
IPC2_protein 3.757
IPC_protein 3.77
Toseland 3.567
ProMoST 3.91
Dawson 3.745
Bjellqvist 3.897
Wikipedia 3.643
Rodwell 3.592
Grimsley 3.465
Solomon 3.732
Lehninger 3.694
Nozaki 3.846
DTASelect 4.037
Thurlkill 3.592
EMBOSS 3.656
Sillero 3.884
Patrickios 1.405
IPC_peptide 3.732
IPC2_peptide 3.872
IPC2.peptide.svr19 3.791
Protein with the highest isoelectric point:
>tr|A0A2L2TAM3|A0A2L2TAM3_9HYPO NAD_binding_2 domain-containing protein OS=Fusarium venenatum OX=56646 PE=4 SV=1
MM1 pKa = 7.86 PLTRR5 pKa = 11.84 THH7 pKa = 6.34 RR8 pKa = 11.84 HH9 pKa = 4.3 SAPRR13 pKa = 11.84 RR14 pKa = 11.84 SIFSTRR20 pKa = 11.84 RR21 pKa = 11.84 RR22 pKa = 11.84 APARR26 pKa = 11.84 SNRR29 pKa = 11.84 HH30 pKa = 4.32 TVTTTTTTTTKK41 pKa = 9.91 PRR43 pKa = 11.84 RR44 pKa = 11.84 GMFGGGSTARR54 pKa = 11.84 RR55 pKa = 11.84 THH57 pKa = 5.96 GAAPVHH63 pKa = 5.35 HH64 pKa = 6.52 HH65 pKa = 5.47 QRR67 pKa = 11.84 RR68 pKa = 11.84 PSMKK72 pKa = 10.02 DD73 pKa = 2.95 KK74 pKa = 11.41 VSGALLKK81 pKa = 10.61 IKK83 pKa = 10.52 GSLTRR88 pKa = 11.84 RR89 pKa = 11.84 PGVKK93 pKa = 9.89 AAGTRR98 pKa = 11.84 RR99 pKa = 11.84 MHH101 pKa = 5.65 GTDD104 pKa = 2.95 GRR106 pKa = 11.84 GARR109 pKa = 11.84 HH110 pKa = 5.73 RR111 pKa = 11.84 RR112 pKa = 11.84 YY113 pKa = 10.37
Molecular weight: 12.63 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.431
IPC2_protein 11.023
IPC_protein 12.515
Toseland 12.676
ProMoST 13.173
Dawson 12.676
Bjellqvist 12.676
Wikipedia 13.159
Rodwell 12.296
Grimsley 12.72
Solomon 13.173
Lehninger 13.086
Nozaki 12.676
DTASelect 12.676
Thurlkill 12.676
EMBOSS 13.173
Sillero 12.676
Patrickios 12.018
IPC_peptide 13.188
IPC2_peptide 12.164
IPC2.peptide.svr19 9.111
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
13945
0
13945
6439375
10
13331
461.8
51.23
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
8.183 ± 0.018
1.314 ± 0.008
5.881 ± 0.017
6.191 ± 0.024
3.783 ± 0.013
6.735 ± 0.02
2.365 ± 0.011
5.153 ± 0.016
5.077 ± 0.019
8.715 ± 0.025
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.267 ± 0.007
3.883 ± 0.01
5.876 ± 0.024
4.032 ± 0.014
5.776 ± 0.021
8.167 ± 0.025
6.128 ± 0.026
6.136 ± 0.016
1.522 ± 0.009
2.817 ± 0.012
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here