Arthrobacter phage Auxilium

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Siphoviridae; unclassified Siphoviridae

Average proteome isoelectric point is 6.19

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 93 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A3G2KA42|A0A3G2KA42_9CAUD DNA methylase OS=Arthrobacter phage Auxilium OX=2419948 GN=77 PE=3 SV=1
MM1 pKa = 7.06THH3 pKa = 6.22YY4 pKa = 10.88CPAQTFAGRR13 pKa = 11.84YY14 pKa = 6.51YY15 pKa = 10.79VEE17 pKa = 4.76PEE19 pKa = 3.66PPEE22 pKa = 3.92YY23 pKa = 10.76CEE25 pKa = 5.37AEE27 pKa = 4.23VEE29 pKa = 4.43EE30 pKa = 4.85EE31 pKa = 4.49GQFCPAHH38 pKa = 6.54EE39 pKa = 4.58EE40 pKa = 3.92QDD42 pKa = 4.74DD43 pKa = 4.01YY44 pKa = 12.1NPWGDD49 pKa = 3.43EE50 pKa = 3.87

Molecular weight: 5.89 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A3G2KA57|A0A3G2KA57_9CAUD MazG-like nucleotide pyrophosphohydrolase OS=Arthrobacter phage Auxilium OX=2419948 GN=82 PE=4 SV=1
MM1 pKa = 7.42KK2 pKa = 9.7PHH4 pKa = 7.23RR5 pKa = 11.84NFDD8 pKa = 3.7SRR10 pKa = 11.84WTVTEE15 pKa = 3.77SGFWEE20 pKa = 4.08WSGALGRR27 pKa = 11.84RR28 pKa = 11.84GYY30 pKa = 10.38GQFWDD35 pKa = 3.68GMRR38 pKa = 11.84TRR40 pKa = 11.84KK41 pKa = 8.7AHH43 pKa = 7.01RR44 pKa = 11.84YY45 pKa = 9.01ALEE48 pKa = 4.35LSTGLNPDD56 pKa = 3.03RR57 pKa = 11.84WTLACHH63 pKa = 6.12TCDD66 pKa = 5.08NPPCVNPGHH75 pKa = 6.86LFWGTPAEE83 pKa = 4.28NSADD87 pKa = 3.41MVAKK91 pKa = 10.34GRR93 pKa = 11.84SSYY96 pKa = 10.06KK97 pKa = 9.44RR98 pKa = 11.84HH99 pKa = 6.01RR100 pKa = 11.84KK101 pKa = 9.03VPPSEE106 pKa = 3.84RR107 pKa = 11.84PKK109 pKa = 9.51IAEE112 pKa = 3.52RR113 pKa = 11.84RR114 pKa = 11.84LRR116 pKa = 11.84GEE118 pKa = 3.7AARR121 pKa = 11.84AIARR125 pKa = 11.84EE126 pKa = 4.16YY127 pKa = 10.62GVSPAAIYY135 pKa = 10.3KK136 pKa = 9.88IVTEE140 pKa = 4.41AGRR143 pKa = 3.57

Molecular weight: 16.28 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

93

0

93

15676

35

1313

168.6

18.47

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

11.904 ± 0.563

1.116 ± 0.166

6.309 ± 0.226

6.596 ± 0.274

2.737 ± 0.169

8.038 ± 0.41

2.316 ± 0.233

4.408 ± 0.159

4.67 ± 0.199

7.732 ± 0.273

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.45 ± 0.135

3.1 ± 0.161

5.384 ± 0.298

3.643 ± 0.171

6.513 ± 0.325

5.378 ± 0.207

6.417 ± 0.205

6.705 ± 0.275

1.875 ± 0.173

2.711 ± 0.136
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski