Camellia oleifera amalgavirus 1
Average proteome isoelectric point is 6.08
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 2 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A2Z6JIM2|A0A2Z6JIM2_9VIRU ORF1+2p OS=Camellia oleifera amalgavirus 1 OX=2069319 GN=ORF1+ORF2 PE=4 SV=1
MM1 pKa = 7.58 ANAGGNGAPRR11 pKa = 11.84 PVQPLPQPPVYY22 pKa = 10.76 YY23 pKa = 10.65 FGQHH27 pKa = 4.96 TPAEE31 pKa = 4.14 NQARR35 pKa = 11.84 ITEE38 pKa = 4.33 AVTFLAQCGVNVGLFTVDD56 pKa = 3.14 AVNQVGMTTDD66 pKa = 3.3 QFIKK70 pKa = 10.35 AVKK73 pKa = 9.34 PLQTHH78 pKa = 6.82 AEE80 pKa = 3.99 QGVINPLFTEE90 pKa = 5.43 AIRR93 pKa = 11.84 ARR95 pKa = 11.84 VFDD98 pKa = 4.32 VVMEE102 pKa = 4.47 LNVQQVVNVCEE113 pKa = 4.09 WLKK116 pKa = 11.27 SRR118 pKa = 11.84 GGQNAISTVYY128 pKa = 9.16 RR129 pKa = 11.84 TRR131 pKa = 11.84 KK132 pKa = 9.16 LQAKK136 pKa = 7.73 VLPGTTAADD145 pKa = 3.76 VAWASVLQQQLSDD158 pKa = 3.56 LSGKK162 pKa = 10.0 RR163 pKa = 11.84 KK164 pKa = 9.43 EE165 pKa = 3.93 IRR167 pKa = 11.84 VEE169 pKa = 3.64 KK170 pKa = 10.83 DD171 pKa = 2.76 EE172 pKa = 5.75 AIAEE176 pKa = 4.15 LRR178 pKa = 11.84 RR179 pKa = 11.84 EE180 pKa = 4.06 IQRR183 pKa = 11.84 LEE185 pKa = 3.86 IEE187 pKa = 4.56 KK188 pKa = 10.91 GLDD191 pKa = 3.02 LAAIDD196 pKa = 4.42 AEE198 pKa = 4.34 MVPASMYY205 pKa = 10.96 NEE207 pKa = 3.9 LNAAEE212 pKa = 4.18 IARR215 pKa = 11.84 RR216 pKa = 11.84 SFNLYY221 pKa = 5.2 TQRR224 pKa = 11.84 AALQGVVPLPRR235 pKa = 11.84 NDD237 pKa = 5.27 DD238 pKa = 4.35 GIKK241 pKa = 9.67 MAVDD245 pKa = 3.65 LFGNEE250 pKa = 3.1 VRR252 pKa = 11.84 QRR254 pKa = 11.84 HH255 pKa = 4.63 MQEE258 pKa = 4.2 FVQGEE263 pKa = 4.57 VVQQQLMQFLRR274 pKa = 11.84 GKK276 pKa = 9.87 ILEE279 pKa = 4.49 LDD281 pKa = 3.76 QIHH284 pKa = 6.32 EE285 pKa = 4.45 RR286 pKa = 11.84 KK287 pKa = 9.07 QASTFRR293 pKa = 11.84 ALLAAVGGEE302 pKa = 4.95 AITQPAVTEE311 pKa = 4.05 EE312 pKa = 4.35 AGDD315 pKa = 4.55 DD316 pKa = 3.9 GDD318 pKa = 4.16 DD319 pKa = 3.73 TGGEE323 pKa = 4.04 AQQAGGEE330 pKa = 4.08 QRR332 pKa = 11.84 QDD334 pKa = 3.08 APEE337 pKa = 4.11 PVPARR342 pKa = 11.84 VAEE345 pKa = 4.16 VGEE348 pKa = 4.69 GSAGDD353 pKa = 3.52 QGAARR358 pKa = 11.84 APPANRR364 pKa = 11.84 GRR366 pKa = 11.84 GRR368 pKa = 11.84 RR369 pKa = 11.84 GQRR372 pKa = 11.84 GVQAGPRR379 pKa = 11.84 KK380 pKa = 9.29 SLRR383 pKa = 11.84 RR384 pKa = 11.84 MGRR387 pKa = 11.84 GEE389 pKa = 4.19 GPSATGRR396 pKa = 11.84 QEE398 pKa = 3.49
Molecular weight: 43.22 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 6.005
IPC2_protein 5.982
IPC_protein 6.008
Toseland 6.287
ProMoST 6.059
Dawson 6.198
Bjellqvist 6.211
Wikipedia 6.173
Rodwell 6.173
Grimsley 6.415
Solomon 6.198
Lehninger 6.186
Nozaki 6.427
DTASelect 6.605
Thurlkill 6.59
EMBOSS 6.547
Sillero 6.532
Patrickios 4.863
IPC_peptide 6.211
IPC2_peptide 6.478
IPC2.peptide.svr19 6.582
Protein with the highest isoelectric point:
>tr|A0A2Z6JIM2|A0A2Z6JIM2_9VIRU ORF1+2p OS=Camellia oleifera amalgavirus 1 OX=2069319 GN=ORF1+ORF2 PE=4 SV=1
MM1 pKa = 7.58 ANAGGNGAPRR11 pKa = 11.84 PVQPLPQPPVYY22 pKa = 10.76 YY23 pKa = 10.65 FGQHH27 pKa = 4.96 TPAEE31 pKa = 4.14 NQARR35 pKa = 11.84 ITEE38 pKa = 4.33 AVTFLAQCGVNVGLFTVDD56 pKa = 3.14 AVNQVGMTTDD66 pKa = 3.3 QFIKK70 pKa = 10.35 AVKK73 pKa = 9.34 PLQTHH78 pKa = 6.82 AEE80 pKa = 3.99 QGVINPLFTEE90 pKa = 5.43 AIRR93 pKa = 11.84 ARR95 pKa = 11.84 VFDD98 pKa = 4.32 VVMEE102 pKa = 4.47 LNVQQVVNVCEE113 pKa = 4.09 WLKK116 pKa = 11.27 SRR118 pKa = 11.84 GGQNAISTVYY128 pKa = 9.16 RR129 pKa = 11.84 TRR131 pKa = 11.84 KK132 pKa = 9.16 LQAKK136 pKa = 7.73 VLPGTTAADD145 pKa = 3.76 VAWASVLQQQLSDD158 pKa = 3.56 LSGKK162 pKa = 10.0 RR163 pKa = 11.84 KK164 pKa = 9.43 EE165 pKa = 3.93 IRR167 pKa = 11.84 VEE169 pKa = 3.64 KK170 pKa = 10.83 DD171 pKa = 2.76 EE172 pKa = 5.75 AIAEE176 pKa = 4.15 LRR178 pKa = 11.84 RR179 pKa = 11.84 EE180 pKa = 4.06 IQRR183 pKa = 11.84 LEE185 pKa = 3.86 IEE187 pKa = 4.56 KK188 pKa = 10.91 GLDD191 pKa = 3.02 LAAIDD196 pKa = 4.42 AEE198 pKa = 4.34 MVPASMYY205 pKa = 10.96 NEE207 pKa = 3.9 LNAAEE212 pKa = 4.18 IARR215 pKa = 11.84 RR216 pKa = 11.84 SFNLYY221 pKa = 5.2 TQRR224 pKa = 11.84 AALQGVVPLPRR235 pKa = 11.84 NDD237 pKa = 5.27 DD238 pKa = 4.35 GIKK241 pKa = 9.67 MAVDD245 pKa = 3.65 LFGNEE250 pKa = 3.1 VRR252 pKa = 11.84 QRR254 pKa = 11.84 HH255 pKa = 4.63 MQEE258 pKa = 4.2 FVQGEE263 pKa = 4.57 VVQQQLMQFLRR274 pKa = 11.84 GKK276 pKa = 9.87 ILEE279 pKa = 4.49 LDD281 pKa = 3.76 QIHH284 pKa = 6.32 EE285 pKa = 4.45 RR286 pKa = 11.84 KK287 pKa = 9.04 QASTFVPYY295 pKa = 9.93 WLQSVVRR302 pKa = 11.84 RR303 pKa = 11.84 LHH305 pKa = 5.94 SRR307 pKa = 11.84 PLQRR311 pKa = 11.84 RR312 pKa = 11.84 LEE314 pKa = 4.15 MMEE317 pKa = 4.57 MIPVGKK323 pKa = 9.25 PNKK326 pKa = 9.47 PGEE329 pKa = 4.36 NNVRR333 pKa = 11.84 MPLSLFLPEE342 pKa = 3.99 WLKK345 pKa = 11.09 SEE347 pKa = 4.26 KK348 pKa = 10.28 GQLGIKK354 pKa = 7.15 VQRR357 pKa = 11.84 GLRR360 pKa = 11.84 RR361 pKa = 11.84 QIEE364 pKa = 4.44 VVEE367 pKa = 4.82 GVASEE372 pKa = 4.06 EE373 pKa = 3.86 FRR375 pKa = 11.84 LVRR378 pKa = 11.84 GKK380 pKa = 11.06 VYY382 pKa = 10.7 VEE384 pKa = 4.05 WAAEE388 pKa = 4.17 KK389 pKa = 10.86 ARR391 pKa = 11.84 LLPVARR397 pKa = 11.84 SKK399 pKa = 11.43 FEE401 pKa = 3.85 TGVRR405 pKa = 11.84 RR406 pKa = 11.84 VIGGGEE412 pKa = 3.63 MRR414 pKa = 11.84 NWHH417 pKa = 6.15 VASSMYY423 pKa = 10.41 RR424 pKa = 11.84 GGGNNADD431 pKa = 4.1 ALRR434 pKa = 11.84 LLSNASFMNPEE445 pKa = 4.35 KK446 pKa = 10.62 YY447 pKa = 10.24 LHH449 pKa = 6.31 EE450 pKa = 4.75 CFTIEE455 pKa = 3.81 TARR458 pKa = 11.84 RR459 pKa = 11.84 EE460 pKa = 4.15 LLLTTDD466 pKa = 3.58 QKK468 pKa = 11.57 VPDD471 pKa = 4.29 GPEE474 pKa = 3.88 SVKK477 pKa = 10.33 MKK479 pKa = 10.61 NFNNDD484 pKa = 2.2 ATAGPFLRR492 pKa = 11.84 AFGIKK497 pKa = 9.73 GKK499 pKa = 10.79 YY500 pKa = 8.16 GLKK503 pKa = 9.28 TLLEE507 pKa = 3.9 QVMWQFYY514 pKa = 10.69 DD515 pKa = 3.76 YY516 pKa = 10.85 YY517 pKa = 11.43 ANNEE521 pKa = 4.02 VSDD524 pKa = 3.69 ATLPFLTARR533 pKa = 11.84 VGFRR537 pKa = 11.84 TKK539 pKa = 11.1 LMTEE543 pKa = 4.43 RR544 pKa = 11.84 KK545 pKa = 9.25 ALEE548 pKa = 3.83 KK549 pKa = 10.74 MMVNDD554 pKa = 4.12 TYY556 pKa = 11.64 GRR558 pKa = 11.84 AVMMLDD564 pKa = 3.68 ALEE567 pKa = 4.23 QAASSPLYY575 pKa = 10.59 NILSSQCFHH584 pKa = 5.75 GRR586 pKa = 11.84 LRR588 pKa = 11.84 RR589 pKa = 11.84 EE590 pKa = 3.85 SGFRR594 pKa = 11.84 NALIRR599 pKa = 11.84 ASSDD603 pKa = 2.54 WHH605 pKa = 5.99 FMWEE609 pKa = 4.36 EE610 pKa = 3.81 VQEE613 pKa = 4.05 AAVIVEE619 pKa = 4.36 LDD621 pKa = 2.89 WSKK624 pKa = 11.03 FDD626 pKa = 3.97 RR627 pKa = 11.84 EE628 pKa = 4.19 RR629 pKa = 11.84 PAEE632 pKa = 4.65 DD633 pKa = 3.09 IEE635 pKa = 5.05 FIINVVLSCFTPKK648 pKa = 9.92 TPRR651 pKa = 11.84 EE652 pKa = 3.93 EE653 pKa = 4.13 RR654 pKa = 11.84 LLRR657 pKa = 11.84 AYY659 pKa = 10.49 GIMMRR664 pKa = 11.84 RR665 pKa = 11.84 ALIEE669 pKa = 3.77 RR670 pKa = 11.84 LMITDD675 pKa = 3.36 NAGVFGISGMVPSGSLWTGWLDD697 pKa = 3.31 TALNILYY704 pKa = 9.74 IRR706 pKa = 11.84 AACRR710 pKa = 11.84 EE711 pKa = 4.2 IGVSDD716 pKa = 3.52 QNSFPKK722 pKa = 10.64 CAGDD726 pKa = 4.37 DD727 pKa = 3.7 NLTLFMRR734 pKa = 11.84 DD735 pKa = 3.17 PGDD738 pKa = 3.41 RR739 pKa = 11.84 ALNRR743 pKa = 11.84 MKK745 pKa = 10.8 DD746 pKa = 3.36 ILNKK750 pKa = 9.28 WFRR753 pKa = 11.84 AGIKK757 pKa = 9.76 EE758 pKa = 4.13 DD759 pKa = 4.03 FFIHH763 pKa = 6.53 RR764 pKa = 11.84 PPYY767 pKa = 9.94 HH768 pKa = 6.04 VSKK771 pKa = 10.42 FQACFPPGTDD781 pKa = 3.58 LSEE784 pKa = 4.45 GTSKK788 pKa = 11.22 LIDD791 pKa = 3.32 EE792 pKa = 4.94 AEE794 pKa = 4.09 WIEE797 pKa = 3.91 FHH799 pKa = 7.87 DD800 pKa = 4.22 EE801 pKa = 4.31 LEE803 pKa = 4.4 VDD805 pKa = 3.83 VSRR808 pKa = 11.84 GKK810 pKa = 7.87 SHH812 pKa = 6.22 RR813 pKa = 11.84 WEE815 pKa = 3.8 YY816 pKa = 10.73 RR817 pKa = 11.84 FKK819 pKa = 11.1 GKK821 pKa = 9.56 PKK823 pKa = 10.28 FLSCYY828 pKa = 7.46 WLEE831 pKa = 3.92 NGRR834 pKa = 11.84 PIRR837 pKa = 11.84 PAADD841 pKa = 3.44 NLEE844 pKa = 4.12 KK845 pKa = 10.87 LLWPEE850 pKa = 4.02 GVHH853 pKa = 7.08 DD854 pKa = 4.2 KK855 pKa = 11.74 LEE857 pKa = 4.18 IYY859 pKa = 9.14 EE860 pKa = 4.18 AAVASMIVDD869 pKa = 3.73 NPWNHH874 pKa = 6.46 HH875 pKa = 5.23 NVNHH879 pKa = 6.71 LLMRR883 pKa = 11.84 YY884 pKa = 9.93 IIIQQIKK891 pKa = 9.44 RR892 pKa = 11.84 LGVGPANVEE901 pKa = 3.96 DD902 pKa = 4.3 VLFLSQFRR910 pKa = 11.84 NEE912 pKa = 4.14 GEE914 pKa = 4.54 EE915 pKa = 3.94 IPFPQVAPWRR925 pKa = 11.84 KK926 pKa = 8.33 FKK928 pKa = 10.7 EE929 pKa = 4.07 HH930 pKa = 6.03 VVMEE934 pKa = 4.55 EE935 pKa = 3.9 YY936 pKa = 10.99 NEE938 pKa = 4.05 VMEE941 pKa = 4.76 HH942 pKa = 6.21 VEE944 pKa = 3.87 NFKK947 pKa = 11.25 NFLSGVTSLYY957 pKa = 10.81 ARR959 pKa = 11.84 RR960 pKa = 11.84 AEE962 pKa = 4.3 GGVDD966 pKa = 2.43 AWKK969 pKa = 10.34 FMEE972 pKa = 4.77 IIRR975 pKa = 11.84 GEE977 pKa = 4.18 SHH979 pKa = 6.42 VGEE982 pKa = 4.29 GQFGNDD988 pKa = 4.45 LIDD991 pKa = 3.33 WLKK994 pKa = 10.07 WMHH997 pKa = 5.45 VHH999 pKa = 7.24 PMTKK1003 pKa = 10.05 FLRR1006 pKa = 11.84 GTRR1009 pKa = 11.84 MFRR1012 pKa = 11.84 TPSEE1016 pKa = 4.2 EE1017 pKa = 3.56 IVAGDD1022 pKa = 4.1 EE1023 pKa = 4.11 EE1024 pKa = 4.19 MDD1026 pKa = 3.76 AAVEE1030 pKa = 3.98 AFNVLIEE1037 pKa = 4.17 RR1038 pKa = 11.84 VEE1040 pKa = 4.09 SEE1042 pKa = 3.97 EE1043 pKa = 4.03 FQDD1046 pKa = 4.11 VEE1048 pKa = 5.5 SFAKK1052 pKa = 9.37 WVSNMLRR1059 pKa = 11.84 EE1060 pKa = 4.36 NVLPHH1065 pKa = 5.35 QQ1066 pKa = 4.24
Molecular weight: 122.02 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 6.154
IPC2_protein 6.084
IPC_protein 6.148
Toseland 6.517
ProMoST 6.491
Dawson 6.427
Bjellqvist 6.427
Wikipedia 6.427
Rodwell 6.415
Grimsley 6.664
Solomon 6.427
Lehninger 6.415
Nozaki 6.693
DTASelect 6.868
Thurlkill 6.898
EMBOSS 6.854
Sillero 6.795
Patrickios 4.609
IPC_peptide 6.44
IPC2_peptide 6.693
IPC2.peptide.svr19 6.717
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
2
0
2
1464
398
1066
732.0
82.62
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
9.016 ± 1.586
0.751 ± 0.12
4.44 ± 0.04
8.538 ± 0.361
4.303 ± 0.741
7.377 ± 1.166
1.844 ± 0.404
4.508 ± 0.356
4.44 ± 0.565
8.402 ± 0.658
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
3.279 ± 0.49
4.577 ± 0.389
4.781 ± 0.238
5.806 ± 1.68
7.923 ± 0.298
4.098 ± 0.642
3.825 ± 0.336
8.402 ± 0.31
1.708 ± 0.58
1.981 ± 0.349
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here