Amino acid dipepetide frequency for Phormidium virus WMP4

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10-3.

For more information see sequence space article on Wikipedia.

AlaCysAspGluPheGlyHisIleLysLeuMetAsnProGlnArgSerThrValTrpTyrXaa
Ala
9.294AlaAla: 9.294 ± 1.217
0.658AlaCys: 0.658 ± 0.284
5.264AlaAsp: 5.264 ± 0.716
5.839AlaGlu: 5.839 ± 0.677
2.221AlaPhe: 2.221 ± 0.415
4.441AlaGly: 4.441 ± 0.627
0.905AlaHis: 0.905 ± 0.233
4.77AlaIle: 4.77 ± 0.62
5.264AlaLys: 5.264 ± 0.879
7.813AlaLeu: 7.813 ± 0.869
2.138AlaMet: 2.138 ± 0.352
4.277AlaAsn: 4.277 ± 0.848
4.194AlaPro: 4.194 ± 0.666
5.346AlaGln: 5.346 ± 1.096
4.77AlaArg: 4.77 ± 0.664
6.168AlaSer: 6.168 ± 0.646
5.839AlaThr: 5.839 ± 0.896
6.826AlaVal: 6.826 ± 0.737
0.987AlaTrp: 0.987 ± 0.258
2.55AlaTyr: 2.55 ± 0.372
0.0AlaXaa: 0.0 ± 0.0
Cys
0.658CysAla: 0.658 ± 0.196
0.0CysCys: 0.0 ± 0.0
0.493CysAsp: 0.493 ± 0.276
0.329CysGlu: 0.329 ± 0.225
0.329CysPhe: 0.329 ± 0.173
0.576CysGly: 0.576 ± 0.203
0.411CysHis: 0.411 ± 0.212
0.576CysIle: 0.576 ± 0.227
0.164CysLys: 0.164 ± 0.129
0.493CysLeu: 0.493 ± 0.182
0.0CysMet: 0.0 ± 0.0
0.329CysAsn: 0.329 ± 0.166
0.329CysPro: 0.329 ± 0.179
0.247CysGln: 0.247 ± 0.146
0.329CysArg: 0.329 ± 0.204
0.329CysSer: 0.329 ± 0.128
0.082CysThr: 0.082 ± 0.074
0.74CysVal: 0.74 ± 0.286
0.164CysTrp: 0.164 ± 0.107
0.247CysTyr: 0.247 ± 0.197
0.0CysXaa: 0.0 ± 0.0
Asp
4.606AspAla: 4.606 ± 0.713
0.411AspCys: 0.411 ± 0.18
4.441AspAsp: 4.441 ± 0.667
3.536AspGlu: 3.536 ± 0.655
2.303AspPhe: 2.303 ± 0.493
3.865AspGly: 3.865 ± 0.636
0.658AspHis: 0.658 ± 0.294
3.454AspIle: 3.454 ± 0.448
2.056AspLys: 2.056 ± 0.379
6.333AspLeu: 6.333 ± 0.613
1.727AspMet: 1.727 ± 0.507
2.138AspAsn: 2.138 ± 0.455
3.783AspPro: 3.783 ± 0.668
1.234AspGln: 1.234 ± 0.254
2.961AspArg: 2.961 ± 0.41
4.03AspSer: 4.03 ± 0.417
4.277AspThr: 4.277 ± 0.622
5.099AspVal: 5.099 ± 0.748
0.822AspTrp: 0.822 ± 0.28
2.55AspTyr: 2.55 ± 0.447
0.0AspXaa: 0.0 ± 0.0
Glu
7.402GluAla: 7.402 ± 1.092
0.329GluCys: 0.329 ± 0.212
2.467GluAsp: 2.467 ± 0.7
3.125GluGlu: 3.125 ± 0.593
2.385GluPhe: 2.385 ± 0.538
2.796GluGly: 2.796 ± 0.456
0.987GluHis: 0.987 ± 0.274
2.467GluIle: 2.467 ± 0.475
2.221GluLys: 2.221 ± 0.524
7.649GluLeu: 7.649 ± 0.849
1.645GluMet: 1.645 ± 0.42
1.234GluAsn: 1.234 ± 0.231
2.056GluPro: 2.056 ± 0.37
3.043GluGln: 3.043 ± 0.48
3.865GluArg: 3.865 ± 0.685
3.454GluSer: 3.454 ± 0.506
4.112GluThr: 4.112 ± 0.726
3.536GluVal: 3.536 ± 0.489
1.069GluTrp: 1.069 ± 0.27
2.879GluTyr: 2.879 ± 0.52
0.0GluXaa: 0.0 ± 0.0
Phe
2.385PheAla: 2.385 ± 0.394
0.329PheCys: 0.329 ± 0.153
3.043PheAsp: 3.043 ± 0.507
1.563PheGlu: 1.563 ± 0.394
1.069PhePhe: 1.069 ± 0.298
2.385PheGly: 2.385 ± 0.491
0.493PheHis: 0.493 ± 0.215
1.727PheIle: 1.727 ± 0.434
2.056PheLys: 2.056 ± 0.492
1.974PheLeu: 1.974 ± 0.342
0.576PheMet: 0.576 ± 0.153
1.974PheAsn: 1.974 ± 0.489
1.234PhePro: 1.234 ± 0.443
1.645PheGln: 1.645 ± 0.303
1.316PheArg: 1.316 ± 0.226
2.55PheSer: 2.55 ± 0.452
3.454PheThr: 3.454 ± 0.746
2.138PheVal: 2.138 ± 0.476
0.329PheTrp: 0.329 ± 0.141
1.234PheTyr: 1.234 ± 0.319
0.0PheXaa: 0.0 ± 0.0
Gly
5.922GlyAla: 5.922 ± 0.726
0.74GlyCys: 0.74 ± 0.27
4.03GlyAsp: 4.03 ± 0.467
3.948GlyGlu: 3.948 ± 0.591
2.796GlyPhe: 2.796 ± 0.358
5.593GlyGly: 5.593 ± 0.691
0.74GlyHis: 0.74 ± 0.281
2.796GlyIle: 2.796 ± 0.473
4.277GlyLys: 4.277 ± 0.626
4.606GlyLeu: 4.606 ± 0.787
2.138GlyMet: 2.138 ± 0.563
3.043GlyAsn: 3.043 ± 0.545
0.576GlyPro: 0.576 ± 0.222
2.221GlyGln: 2.221 ± 0.439
3.865GlyArg: 3.865 ± 0.543
4.935GlySer: 4.935 ± 0.724
3.29GlyThr: 3.29 ± 0.487
6.579GlyVal: 6.579 ± 0.684
1.316GlyTrp: 1.316 ± 0.35
2.467GlyTyr: 2.467 ± 0.472
0.0GlyXaa: 0.0 ± 0.0
His
0.822HisAla: 0.822 ± 0.202
0.247HisCys: 0.247 ± 0.162
0.987HisAsp: 0.987 ± 0.259
0.905HisGlu: 0.905 ± 0.264
0.411HisPhe: 0.411 ± 0.187
1.316HisGly: 1.316 ± 0.352
0.082HisHis: 0.082 ± 0.083
0.822HisIle: 0.822 ± 0.254
0.493HisLys: 0.493 ± 0.24
1.727HisLeu: 1.727 ± 0.358
0.247HisMet: 0.247 ± 0.137
0.658HisAsn: 0.658 ± 0.242
1.151HisPro: 1.151 ± 0.393
0.329HisGln: 0.329 ± 0.157
0.987HisArg: 0.987 ± 0.266
0.987HisSer: 0.987 ± 0.273
1.398HisThr: 1.398 ± 0.32
0.822HisVal: 0.822 ± 0.253
0.164HisTrp: 0.164 ± 0.113
1.069HisTyr: 1.069 ± 0.372
0.0HisXaa: 0.0 ± 0.0
Ile
4.606IleAla: 4.606 ± 0.738
0.082IleCys: 0.082 ± 0.079
4.03IleAsp: 4.03 ± 0.736
3.208IleGlu: 3.208 ± 0.596
1.069IlePhe: 1.069 ± 0.31
3.125IleGly: 3.125 ± 0.645
0.905IleHis: 0.905 ± 0.356
2.138IleIle: 2.138 ± 0.459
2.303IleLys: 2.303 ± 0.528
3.783IleLeu: 3.783 ± 0.463
0.905IleMet: 0.905 ± 0.206
1.809IleAsn: 1.809 ± 0.377
3.454IlePro: 3.454 ± 0.457
2.961IleGln: 2.961 ± 0.401
2.714IleArg: 2.714 ± 0.497
3.208IleSer: 3.208 ± 0.522
2.961IleThr: 2.961 ± 0.536
2.303IleVal: 2.303 ± 0.412
0.329IleTrp: 0.329 ± 0.135
1.398IleTyr: 1.398 ± 0.42
0.0IleXaa: 0.0 ± 0.0
Lys
4.441LysAla: 4.441 ± 0.673
0.082LysCys: 0.082 ± 0.098
2.385LysAsp: 2.385 ± 0.485
2.55LysGlu: 2.55 ± 0.449
1.809LysPhe: 1.809 ± 0.42
2.303LysGly: 2.303 ± 0.416
0.658LysHis: 0.658 ± 0.215
2.221LysIle: 2.221 ± 0.345
1.809LysLys: 1.809 ± 0.422
5.593LysLeu: 5.593 ± 0.891
1.563LysMet: 1.563 ± 0.314
1.234LysAsn: 1.234 ± 0.253
2.796LysPro: 2.796 ± 0.525
2.056LysGln: 2.056 ± 0.341
3.948LysArg: 3.948 ± 0.774
3.536LysSer: 3.536 ± 0.752
3.372LysThr: 3.372 ± 0.478
2.632LysVal: 2.632 ± 0.45
0.905LysTrp: 0.905 ± 0.326
2.467LysTyr: 2.467 ± 0.341
0.0LysXaa: 0.0 ± 0.0
Leu
9.376LeuAla: 9.376 ± 1.035
0.658LeuCys: 0.658 ± 0.244
5.839LeuAsp: 5.839 ± 0.737
6.579LeuGlu: 6.579 ± 0.646
2.467LeuPhe: 2.467 ± 0.494
5.922LeuGly: 5.922 ± 0.626
2.632LeuHis: 2.632 ± 0.445
3.948LeuIle: 3.948 ± 0.439
5.099LeuLys: 5.099 ± 0.665
6.333LeuLeu: 6.333 ± 0.999
2.961LeuMet: 2.961 ± 0.505
4.03LeuAsn: 4.03 ± 0.443
4.77LeuPro: 4.77 ± 0.574
5.839LeuGln: 5.839 ± 0.687
5.51LeuArg: 5.51 ± 0.528
6.662LeuSer: 6.662 ± 0.719
5.264LeuThr: 5.264 ± 0.646
4.03LeuVal: 4.03 ± 0.591
1.398LeuTrp: 1.398 ± 0.369
2.796LeuTyr: 2.796 ± 0.504
0.0LeuXaa: 0.0 ± 0.0
Met
1.974MetAla: 1.974 ± 0.391
0.247MetCys: 0.247 ± 0.143
1.151MetAsp: 1.151 ± 0.337
0.905MetGlu: 0.905 ± 0.227
0.658MetPhe: 0.658 ± 0.223
2.714MetGly: 2.714 ± 0.565
0.082MetHis: 0.082 ± 0.09
0.493MetIle: 0.493 ± 0.206
0.822MetLys: 0.822 ± 0.266
2.55MetLeu: 2.55 ± 0.612
1.234MetMet: 1.234 ± 0.276
1.398MetAsn: 1.398 ± 0.297
2.632MetPro: 2.632 ± 0.449
1.974MetGln: 1.974 ± 0.424
1.151MetArg: 1.151 ± 0.289
1.892MetSer: 1.892 ± 0.374
2.385MetThr: 2.385 ± 0.552
1.316MetVal: 1.316 ± 0.326
0.329MetTrp: 0.329 ± 0.154
0.987MetTyr: 0.987 ± 0.352
0.0MetXaa: 0.0 ± 0.0
Asn
3.536AsnAla: 3.536 ± 0.715
0.411AsnCys: 0.411 ± 0.227
1.727AsnAsp: 1.727 ± 0.276
1.645AsnGlu: 1.645 ± 0.439
1.563AsnPhe: 1.563 ± 0.314
3.125AsnGly: 3.125 ± 0.559
0.576AsnHis: 0.576 ± 0.16
2.385AsnIle: 2.385 ± 0.414
1.809AsnLys: 1.809 ± 0.316
4.688AsnLeu: 4.688 ± 0.748
0.822AsnMet: 0.822 ± 0.27
1.974AsnAsn: 1.974 ± 0.543
2.632AsnPro: 2.632 ± 0.48
2.138AsnGln: 2.138 ± 0.439
2.796AsnArg: 2.796 ± 0.454
3.043AsnSer: 3.043 ± 0.421
3.536AsnThr: 3.536 ± 0.639
2.879AsnVal: 2.879 ± 0.508
0.493AsnTrp: 0.493 ± 0.232
1.645AsnTyr: 1.645 ± 0.417
0.0AsnXaa: 0.0 ± 0.0
Pro
3.701ProAla: 3.701 ± 0.408
0.082ProCys: 0.082 ± 0.071
3.29ProAsp: 3.29 ± 0.497
3.701ProGlu: 3.701 ± 0.692
1.316ProPhe: 1.316 ± 0.496
2.55ProGly: 2.55 ± 0.45
1.234ProHis: 1.234 ± 0.325
2.55ProIle: 2.55 ± 0.527
2.056ProLys: 2.056 ± 0.497
3.372ProLeu: 3.372 ± 0.595
1.48ProMet: 1.48 ± 0.294
2.138ProAsn: 2.138 ± 0.536
4.277ProPro: 4.277 ± 1.003
2.55ProGln: 2.55 ± 0.484
2.138ProArg: 2.138 ± 0.342
3.536ProSer: 3.536 ± 0.502
4.852ProThr: 4.852 ± 0.757
4.194ProVal: 4.194 ± 0.602
0.576ProTrp: 0.576 ± 0.219
1.645ProTyr: 1.645 ± 0.436
0.0ProXaa: 0.0 ± 0.0
Gln
7.402GlnAla: 7.402 ± 1.299
0.247GlnCys: 0.247 ± 0.12
1.809GlnAsp: 1.809 ± 0.327
2.632GlnGlu: 2.632 ± 0.628
2.221GlnPhe: 2.221 ± 0.377
2.961GlnGly: 2.961 ± 0.415
0.576GlnHis: 0.576 ± 0.223
2.138GlnIle: 2.138 ± 0.368
1.563GlnLys: 1.563 ± 0.33
6.744GlnLeu: 6.744 ± 0.76
1.48GlnMet: 1.48 ± 0.352
2.138GlnAsn: 2.138 ± 0.48
2.714GlnPro: 2.714 ± 0.579
5.922GlnGln: 5.922 ± 1.393
3.125GlnArg: 3.125 ± 0.582
2.796GlnSer: 2.796 ± 0.49
3.043GlnThr: 3.043 ± 0.449
3.372GlnVal: 3.372 ± 0.453
0.411GlnTrp: 0.411 ± 0.188
1.234GlnTyr: 1.234 ± 0.258
0.0GlnXaa: 0.0 ± 0.0
Arg
4.03ArgAla: 4.03 ± 0.481
0.164ArgCys: 0.164 ± 0.131
2.55ArgAsp: 2.55 ± 0.508
4.359ArgGlu: 4.359 ± 0.711
1.974ArgPhe: 1.974 ± 0.395
3.536ArgGly: 3.536 ± 0.706
1.069ArgHis: 1.069 ± 0.333
3.125ArgIle: 3.125 ± 0.598
3.208ArgLys: 3.208 ± 0.517
5.51ArgLeu: 5.51 ± 0.917
1.48ArgMet: 1.48 ± 0.356
3.372ArgAsn: 3.372 ± 0.623
2.056ArgPro: 2.056 ± 0.375
4.194ArgGln: 4.194 ± 0.625
3.29ArgArg: 3.29 ± 0.513
2.303ArgSer: 2.303 ± 0.453
2.714ArgThr: 2.714 ± 0.317
4.112ArgVal: 4.112 ± 0.574
0.987ArgTrp: 0.987 ± 0.359
1.892ArgTyr: 1.892 ± 0.512
0.0ArgXaa: 0.0 ± 0.0
Ser
4.852SerAla: 4.852 ± 0.561
0.576SerCys: 0.576 ± 0.235
3.372SerAsp: 3.372 ± 0.472
3.043SerGlu: 3.043 ± 0.464
2.385SerPhe: 2.385 ± 0.453
4.359SerGly: 4.359 ± 0.741
0.905SerHis: 0.905 ± 0.343
3.454SerIle: 3.454 ± 0.479
3.536SerLys: 3.536 ± 0.454
5.757SerLeu: 5.757 ± 0.75
1.892SerMet: 1.892 ± 0.431
3.208SerAsn: 3.208 ± 0.599
3.454SerPro: 3.454 ± 0.487
2.714SerGln: 2.714 ± 0.542
3.372SerArg: 3.372 ± 0.579
4.935SerSer: 4.935 ± 0.748
4.77SerThr: 4.77 ± 0.541
4.359SerVal: 4.359 ± 0.959
0.658SerTrp: 0.658 ± 0.201
3.125SerTyr: 3.125 ± 0.575
0.0SerXaa: 0.0 ± 0.0
Thr
5.428ThrAla: 5.428 ± 0.78
0.658ThrCys: 0.658 ± 0.272
4.852ThrAsp: 4.852 ± 0.621
3.208ThrGlu: 3.208 ± 0.722
2.303ThrPhe: 2.303 ± 0.362
5.181ThrGly: 5.181 ± 0.616
1.069ThrHis: 1.069 ± 0.282
3.29ThrIle: 3.29 ± 0.469
2.55ThrLys: 2.55 ± 0.634
6.251ThrLeu: 6.251 ± 1.056
1.316ThrMet: 1.316 ± 0.297
1.974ThrAsn: 1.974 ± 0.394
4.606ThrPro: 4.606 ± 0.447
3.701ThrGln: 3.701 ± 0.616
3.372ThrArg: 3.372 ± 0.468
3.865ThrSer: 3.865 ± 0.518
4.359ThrThr: 4.359 ± 0.583
4.03ThrVal: 4.03 ± 0.738
0.987ThrTrp: 0.987 ± 0.411
2.55ThrTyr: 2.55 ± 0.568
0.0ThrXaa: 0.0 ± 0.0
Val
4.935ValAla: 4.935 ± 0.647
0.329ValCys: 0.329 ± 0.145
4.441ValAsp: 4.441 ± 0.616
4.935ValGlu: 4.935 ± 0.605
2.303ValPhe: 2.303 ± 0.412
4.277ValGly: 4.277 ± 0.811
0.905ValHis: 0.905 ± 0.273
2.632ValIle: 2.632 ± 0.424
4.441ValLys: 4.441 ± 0.588
5.839ValLeu: 5.839 ± 0.722
2.056ValMet: 2.056 ± 0.31
4.277ValAsn: 4.277 ± 0.486
2.961ValPro: 2.961 ± 0.385
3.619ValGln: 3.619 ± 0.523
3.619ValArg: 3.619 ± 0.543
3.783ValSer: 3.783 ± 0.652
3.208ValThr: 3.208 ± 0.692
5.428ValVal: 5.428 ± 0.591
1.069ValTrp: 1.069 ± 0.297
2.632ValTyr: 2.632 ± 0.458
0.0ValXaa: 0.0 ± 0.0
Trp
1.398TrpAla: 1.398 ± 0.325
0.493TrpCys: 0.493 ± 0.23
1.151TrpAsp: 1.151 ± 0.365
0.74TrpGlu: 0.74 ± 0.241
0.658TrpPhe: 0.658 ± 0.198
1.151TrpGly: 1.151 ± 0.307
0.0TrpHis: 0.0 ± 0.0
0.329TrpIle: 0.329 ± 0.156
0.329TrpLys: 0.329 ± 0.17
1.645TrpLeu: 1.645 ± 0.345
0.247TrpMet: 0.247 ± 0.148
0.822TrpAsn: 0.822 ± 0.28
0.0TrpPro: 0.0 ± 0.0
1.069TrpGln: 1.069 ± 0.212
0.905TrpArg: 0.905 ± 0.293
0.658TrpSer: 0.658 ± 0.239
0.493TrpThr: 0.493 ± 0.197
0.822TrpVal: 0.822 ± 0.347
0.329TrpTrp: 0.329 ± 0.153
0.905TrpTyr: 0.905 ± 0.258
0.0TrpXaa: 0.0 ± 0.0
Tyr
2.796TyrAla: 2.796 ± 0.397
0.082TyrCys: 0.082 ± 0.095
2.961TyrAsp: 2.961 ± 0.481
1.974TyrGlu: 1.974 ± 0.363
1.234TyrPhe: 1.234 ± 0.33
3.701TyrGly: 3.701 ± 0.637
0.74TyrHis: 0.74 ± 0.427
1.974TyrIle: 1.974 ± 0.383
2.303TyrLys: 2.303 ± 0.366
3.454TyrLeu: 3.454 ± 0.549
0.905TyrMet: 0.905 ± 0.287
1.48TyrAsn: 1.48 ± 0.512
1.563TyrPro: 1.563 ± 0.463
1.727TyrGln: 1.727 ± 0.381
1.892TyrArg: 1.892 ± 0.404
2.056TyrSer: 2.056 ± 0.376
2.138TyrThr: 2.138 ± 0.515
2.467TyrVal: 2.467 ± 0.474
0.822TyrTrp: 0.822 ± 0.338
1.48TyrTyr: 1.48 ± 0.434
0.0TyrXaa: 0.0 ± 0.0
Xaa
0.0XaaAla: 0.0 ± 0.0
0.0XaaCys: 0.0 ± 0.0
0.0XaaAsp: 0.0 ± 0.0
0.0XaaGlu: 0.0 ± 0.0
0.0XaaPhe: 0.0 ± 0.0
0.0XaaGly: 0.0 ± 0.0
0.0XaaHis: 0.0 ± 0.0
0.0XaaIle: 0.0 ± 0.0
0.0XaaLys: 0.0 ± 0.0
0.0XaaLeu: 0.0 ± 0.0
0.0XaaMet: 0.0 ± 0.0
0.0XaaAsn: 0.0 ± 0.0
0.0XaaPro: 0.0 ± 0.0
0.0XaaGln: 0.0 ± 0.0
0.0XaaArg: 0.0 ± 0.0
0.0XaaSer: 0.0 ± 0.0
0.0XaaThr: 0.0 ± 0.0
0.0XaaVal: 0.0 ± 0.0
0.0XaaTrp: 0.0 ± 0.0
0.0XaaTyr: 0.0 ± 0.0
0.0XaaXaa: 0.0 ± 0.0
Statistics based on 45 proteins (12160 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski