Aeropyrum pernix ovoid virus 1 (APOV1)

Taxonomy: Viruses; Guttaviridae; Betaguttavirus

Average proteome isoelectric point is 7.65

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 21 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|G3CAV4|G3CAV4_APOV1 Uncharacterized protein OS=Aeropyrum pernix ovoid virus 1 OX=1032474 PE=4 SV=1
MM1 pKa = 7.76KK2 pKa = 10.46LPLVAFAIAIALLVAPAAPALAAASVTIDD31 pKa = 3.21ATVVNQTYY39 pKa = 10.28SFPVDD44 pKa = 3.77PPALITVAWSGLQVNIDD61 pKa = 3.66STTNAPATDD70 pKa = 3.53NLALSFRR77 pKa = 11.84DD78 pKa = 3.48SAANEE83 pKa = 3.71VLILFFKK90 pKa = 10.29TSGSIVLQAPALGVTTDD107 pKa = 3.44VVLGDD112 pKa = 3.93WSQLAGLTIKK122 pKa = 10.39YY123 pKa = 9.92RR124 pKa = 11.84QDD126 pKa = 2.81KK127 pKa = 9.51LQIYY131 pKa = 10.19DD132 pKa = 3.47DD133 pKa = 4.63AGNLLYY139 pKa = 10.66EE140 pKa = 4.73LANINAPSLADD151 pKa = 3.43IWAHH155 pKa = 5.5TPDD158 pKa = 3.52GTTQAFTAGSITLSTEE174 pKa = 3.51PDD176 pKa = 3.35YY177 pKa = 11.72SAMYY181 pKa = 9.87DD182 pKa = 3.39PSAITEE188 pKa = 4.01AVAAVMPLLVVVAVFGVVIKK208 pKa = 10.75ILDD211 pKa = 3.61RR212 pKa = 11.84MVGIVGKK219 pKa = 10.42LGRR222 pKa = 4.33

Molecular weight: 23.2 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|G3CAU4|G3CAU4_APOV1 Uncharacterized protein OS=Aeropyrum pernix ovoid virus 1 OX=1032474 PE=4 SV=1
MM1 pKa = 7.28LTLNSLAPSEE11 pKa = 4.23RR12 pKa = 11.84RR13 pKa = 11.84TLILIYY19 pKa = 9.89RR20 pKa = 11.84YY21 pKa = 9.66VYY23 pKa = 10.53RR24 pKa = 11.84EE25 pKa = 3.85GRR27 pKa = 11.84TSRR30 pKa = 11.84CFTYY34 pKa = 10.18QGLRR38 pKa = 11.84SWIHH42 pKa = 4.89YY43 pKa = 8.17ALPKK47 pKa = 10.03HH48 pKa = 6.12EE49 pKa = 4.3RR50 pKa = 11.84PEE52 pKa = 3.65WHH54 pKa = 5.46TVEE57 pKa = 3.83RR58 pKa = 11.84AIRR61 pKa = 11.84RR62 pKa = 11.84LAEE65 pKa = 3.96LEE67 pKa = 4.02VVRR70 pKa = 11.84RR71 pKa = 11.84LRR73 pKa = 11.84RR74 pKa = 11.84GRR76 pKa = 11.84KK77 pKa = 9.48VIFCPGKK84 pKa = 9.18HH85 pKa = 4.11WHH87 pKa = 5.83QLLVEE92 pKa = 4.04YY93 pKa = 10.55RR94 pKa = 11.84GVLSQAVKK102 pKa = 10.77LEE104 pKa = 3.85EE105 pKa = 4.49PYY107 pKa = 10.66RR108 pKa = 11.84SLLHH112 pKa = 5.91YY113 pKa = 10.84LPGHH117 pKa = 5.23KK118 pKa = 8.83TWDD121 pKa = 2.97RR122 pKa = 11.84MRR124 pKa = 11.84NRR126 pKa = 11.84GII128 pKa = 3.6

Molecular weight: 15.59 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

21

0

21

4101

57

678

195.3

22.03

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

10.046 ± 0.981

0.61 ± 0.179

4.779 ± 0.671

6.535 ± 0.684

2.731 ± 0.274

7.364 ± 0.377

1.366 ± 0.233

5.023 ± 0.426

4.999 ± 0.53

12.143 ± 0.68

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.17 ± 0.293

2.17 ± 0.246

4.414 ± 0.279

2.414 ± 0.194

7.925 ± 1.057

5.852 ± 0.411

4.097 ± 0.388

7.608 ± 0.445

2.512 ± 0.32

5.243 ± 0.881
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski