Salmonella phage SPAsTU

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Myoviridae; Seoulvirus; unclassified Seoulvirus

Average proteome isoelectric point is 6.03

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 287 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A2U8UXT0|A0A2U8UXT0_9CAUD Site-specific DNA-methyltransferase (adenine-specific) OS=Salmonella phage SPAsTU OX=2136797 GN=MLDJOKPK_00052 PE=3 SV=2
MM1 pKa = 7.57IDD3 pKa = 3.54IYY5 pKa = 10.96TDD7 pKa = 3.37YY8 pKa = 11.55AAVLTVNRR16 pKa = 11.84HH17 pKa = 4.88EE18 pKa = 5.16DD19 pKa = 3.08RR20 pKa = 11.84AAPMLDD26 pKa = 3.5LVSLGMDD33 pKa = 3.41YY34 pKa = 11.06GYY36 pKa = 11.08DD37 pKa = 3.33VALSDD42 pKa = 4.77VYY44 pKa = 10.74FNPLSEE50 pKa = 4.29PTEE53 pKa = 3.84EE54 pKa = 4.14TVRR57 pKa = 11.84LEE59 pKa = 4.16GIIVKK64 pKa = 9.99VAVALGNRR72 pKa = 11.84LGVGLNPQIVFKK84 pKa = 10.79KK85 pKa = 9.48PKK87 pKa = 7.22EE88 pKa = 4.16TVRR91 pKa = 11.84ILHH94 pKa = 6.12GLLEE98 pKa = 4.34KK99 pKa = 10.85FEE101 pKa = 4.45EE102 pKa = 4.85FEE104 pKa = 4.99DD105 pKa = 3.87TDD107 pKa = 3.71TLYY110 pKa = 11.48GIVLSGEE117 pKa = 3.99TPEE120 pKa = 4.83YY121 pKa = 10.24ILEE124 pKa = 4.11NMCRR128 pKa = 11.84YY129 pKa = 10.17VYY131 pKa = 11.07GDD133 pKa = 4.28DD134 pKa = 3.69NLHH137 pKa = 6.5FEE139 pKa = 4.55DD140 pKa = 6.09LITVVSPRR148 pKa = 11.84VMTVMQNFLSASALEE163 pKa = 4.25AQNAGNDD170 pKa = 3.58YY171 pKa = 10.35LRR173 pKa = 11.84QGRR176 pKa = 11.84VVAYY180 pKa = 10.16LRR182 pKa = 11.84AFPEE186 pKa = 4.21NPSAFVFLNLPPEE199 pKa = 4.63PDD201 pKa = 3.19LTVVQQSFDD210 pKa = 3.6YY211 pKa = 10.94AADD214 pKa = 5.13DD215 pKa = 3.66ISEE218 pKa = 4.11EE219 pKa = 4.49DD220 pKa = 3.86LLIMYY225 pKa = 9.78SVGLSIIPNEE235 pKa = 4.5TFDD238 pKa = 4.37EE239 pKa = 5.18AYY241 pKa = 10.68DD242 pKa = 3.9DD243 pKa = 4.06LAKK246 pKa = 11.04NFALLNAEE254 pKa = 4.4NLPAGDD260 pKa = 4.09LLRR263 pKa = 11.84RR264 pKa = 11.84AMGALKK270 pKa = 10.31AIYY273 pKa = 10.34GMDD276 pKa = 3.82DD277 pKa = 3.34EE278 pKa = 5.39QEE280 pKa = 4.2EE281 pKa = 4.11EE282 pKa = 5.58DD283 pKa = 4.29YY284 pKa = 11.5EE285 pKa = 4.25QDD287 pKa = 3.1

Molecular weight: 32.24 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A345BKW7|A0A345BKW7_9CAUD Uncharacterized protein OS=Salmonella phage SPAsTU OX=2136797 GN=MLDJOKPK_00093 PE=4 SV=1
MM1 pKa = 7.15NALRR5 pKa = 11.84SHH7 pKa = 7.28LLNSQVKK14 pKa = 10.05RR15 pKa = 11.84IQFNQRR21 pKa = 11.84VQQLTAPKK29 pKa = 9.2QNTVRR34 pKa = 11.84RR35 pKa = 11.84IKK37 pKa = 10.98GLGGDD42 pKa = 3.69IIFNKK47 pKa = 10.16RR48 pKa = 2.91

Molecular weight: 5.59 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

287

0

287

77953

30

2377

271.6

30.57

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

7.773 ± 0.156

0.936 ± 0.058

6.46 ± 0.104

6.286 ± 0.158

3.973 ± 0.071

6.592 ± 0.184

1.982 ± 0.077

5.56 ± 0.099

5.272 ± 0.126

9.031 ± 0.121

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.782 ± 0.073

4.939 ± 0.087

4.521 ± 0.104

3.678 ± 0.081

5.524 ± 0.118

5.549 ± 0.101

6.499 ± 0.165

7.077 ± 0.127

1.506 ± 0.061

4.059 ± 0.088
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski