Tamaricihabitans halophyticus
Average proteome isoelectric point is 6.1
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 5978 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A4R2RAZ5|A0A4R2RAZ5_9PSEU Uncharacterized protein DUF4177 OS=Tamaricihabitans halophyticus OX=1262583 GN=EV191_101829 PE=4 SV=1
MM1 pKa = 7.95 RR2 pKa = 11.84 ARR4 pKa = 11.84 SLVVGLLAGAGLVLASVMGPAAAAAPAAEE33 pKa = 4.27 NPNDD37 pKa = 3.63 GVTPYY42 pKa = 10.48 IIGGEE47 pKa = 4.25 DD48 pKa = 3.47 ADD50 pKa = 3.76 QEE52 pKa = 4.86 YY53 pKa = 9.57 PFMTSLQDD61 pKa = 3.72 SQGHH65 pKa = 5.14 FCGGSLISSEE75 pKa = 4.18 WVLTAAHH82 pKa = 6.75 CVQGSAPGDD91 pKa = 3.35 FTARR95 pKa = 11.84 IGSNDD100 pKa = 3.08 NSQGGEE106 pKa = 3.84 EE107 pKa = 4.17 AGVTEE112 pKa = 4.78 VVPHH116 pKa = 7.09 PDD118 pKa = 3.04 YY119 pKa = 11.61 DD120 pKa = 4.06 GASPGADD127 pKa = 2.57 IALVKK132 pKa = 10.24 LDD134 pKa = 4.43 KK135 pKa = 10.77 AVQAAPIKK143 pKa = 9.48 VTGSTEE149 pKa = 3.63 AGTASRR155 pKa = 11.84 LLGWGQTCPEE165 pKa = 4.29 QGCGDD170 pKa = 4.38 VPTMLQQLDD179 pKa = 3.97 TSVVAAEE186 pKa = 4.24 EE187 pKa = 4.4 CTGGIDD193 pKa = 5.33 GSTEE197 pKa = 3.97 LCTDD201 pKa = 3.64 NPNGDD206 pKa = 3.37 SGACYY211 pKa = 10.05 GDD213 pKa = 3.2 SGGPQIVQSGDD224 pKa = 2.26 GWTQIGVTSRR234 pKa = 11.84 TGNNDD239 pKa = 3.03 PTCATGPSIYY249 pKa = 9.69 TNAVAYY255 pKa = 8.63 TDD257 pKa = 4.6 WINEE261 pKa = 4.21 TTGGALAAA269 pKa = 4.86
Molecular weight: 26.98 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.796
IPC2_protein 3.795
IPC_protein 3.783
Toseland 3.567
ProMoST 3.948
Dawson 3.783
Bjellqvist 3.935
Wikipedia 3.706
Rodwell 3.617
Grimsley 3.478
Solomon 3.77
Lehninger 3.719
Nozaki 3.897
DTASelect 4.126
Thurlkill 3.63
EMBOSS 3.719
Sillero 3.91
Patrickios 0.375
IPC_peptide 3.77
IPC2_peptide 3.884
IPC2.peptide.svr19 3.808
Protein with the highest isoelectric point:
>tr|A0A4R2QZ34|A0A4R2QZ34_9PSEU Sugar/nucleoside kinase (Ribokinase family) OS=Tamaricihabitans halophyticus OX=1262583 GN=EV191_102157 PE=4 SV=1
MM1 pKa = 7.66 KK2 pKa = 10.1 VRR4 pKa = 11.84 KK5 pKa = 9.11 SLRR8 pKa = 11.84 SLKK11 pKa = 9.97 NRR13 pKa = 11.84 PGSQVVRR20 pKa = 11.84 RR21 pKa = 11.84 HH22 pKa = 5.11 GKK24 pKa = 9.21 VLVINKK30 pKa = 9.15 RR31 pKa = 11.84 NPRR34 pKa = 11.84 FKK36 pKa = 10.78 ARR38 pKa = 11.84 QGG40 pKa = 3.28
Molecular weight: 4.7 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.494
IPC2_protein 11.111
IPC_protein 12.691
Toseland 12.852
ProMoST 13.349
Dawson 12.852
Bjellqvist 12.852
Wikipedia 13.334
Rodwell 12.618
Grimsley 12.896
Solomon 13.349
Lehninger 13.247
Nozaki 12.852
DTASelect 12.852
Thurlkill 12.852
EMBOSS 13.349
Sillero 12.852
Patrickios 12.34
IPC_peptide 13.349
IPC2_peptide 12.34
IPC2.peptide.svr19 9.103
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
5978
0
5978
1946036
26
23820
325.5
34.95
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
13.272 ± 0.044
0.797 ± 0.013
5.832 ± 0.029
6.039 ± 0.031
2.851 ± 0.021
9.09 ± 0.031
2.131 ± 0.015
3.812 ± 0.026
1.889 ± 0.019
10.629 ± 0.043
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
1.828 ± 0.016
2.034 ± 0.014
5.623 ± 0.028
3.16 ± 0.02
7.742 ± 0.03
5.326 ± 0.023
5.898 ± 0.021
8.491 ± 0.032
1.487 ± 0.012
2.069 ± 0.015
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here