Desulfopila sp. IMCC35006

Taxonomy: cellular organisms; Bacteria; Proteobacteria; delta/epsilon subdivisions; Deltaproteobacteria; Desulfobacterales; Desulfobulbaceae; Desulfopila; unclassified Desulfopila

Average proteome isoelectric point is 6.58

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 4729 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A4U1AP07|A0A4U1AP07_9DELT Uncharacterized protein OS=Desulfopila sp. IMCC35006 OX=2569542 GN=FCL47_03085 PE=4 SV=1
MM1 pKa = 7.87DD2 pKa = 4.09KK3 pKa = 10.03WVCTICGYY11 pKa = 11.06VYY13 pKa = 10.57DD14 pKa = 4.79PEE16 pKa = 6.54KK17 pKa = 10.86GDD19 pKa = 3.97PDD21 pKa = 3.71NGVNPGTAWEE31 pKa = 4.59DD32 pKa = 3.67VPGDD36 pKa = 4.05WLCPICGASKK46 pKa = 11.15DD47 pKa = 4.01DD48 pKa = 4.08FEE50 pKa = 6.55KK51 pKa = 10.93EE52 pKa = 3.83AA53 pKa = 4.81

Molecular weight: 5.84 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A4U1AEN8|A0A4U1AEN8_9DELT MarR family transcriptional regulator OS=Desulfopila sp. IMCC35006 OX=2569542 GN=FCL47_15890 PE=4 SV=1
MM1 pKa = 7.69SKK3 pKa = 9.01RR4 pKa = 11.84TFQPSNLKK12 pKa = 10.2RR13 pKa = 11.84KK14 pKa = 7.48RR15 pKa = 11.84THH17 pKa = 5.98GFRR20 pKa = 11.84EE21 pKa = 4.11RR22 pKa = 11.84MSTRR26 pKa = 11.84AGRR29 pKa = 11.84AVIRR33 pKa = 11.84SRR35 pKa = 11.84RR36 pKa = 11.84AKK38 pKa = 9.51GRR40 pKa = 11.84KK41 pKa = 8.92RR42 pKa = 11.84LSAA45 pKa = 3.96

Molecular weight: 5.33 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

4729

0

4729

1606768

29

4195

339.8

37.68

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

8.624 ± 0.032

1.294 ± 0.016

5.349 ± 0.029

6.072 ± 0.031

4.292 ± 0.022

7.433 ± 0.035

2.146 ± 0.017

6.845 ± 0.03

5.422 ± 0.034

10.431 ± 0.042

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.617 ± 0.015

3.709 ± 0.025

4.327 ± 0.021

3.816 ± 0.021

5.298 ± 0.03

6.015 ± 0.025

5.439 ± 0.03

6.848 ± 0.031

1.106 ± 0.015

2.918 ± 0.018
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski