Nitrosopumilales archaeon

Taxonomy: cellular organisms; Archaea; TACK group; Thaumarchaeota; Nitrosopumilales; unclassified Nitrosopumilales

Average proteome isoelectric point is 7.13

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 1495 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A6A8FJS0|A0A6A8FJS0_9ARCH 50S ribosomal protein L23 OS=Nitrosopumilales archaeon OX=2026768 GN=rpl23 PE=3 SV=1
MM1 pKa = 7.58GSRR4 pKa = 11.84FGGYY8 pKa = 8.92EE9 pKa = 3.38IPGDD13 pKa = 3.97VFSSPCLVDD22 pKa = 3.63DD23 pKa = 5.43AIIVVGSMNNGIYY36 pKa = 10.52ALEE39 pKa = 4.78GYY41 pKa = 9.42NGNVRR46 pKa = 11.84WKK48 pKa = 10.35LEE50 pKa = 3.9TGGEE54 pKa = 4.45VWSSPSYY61 pKa = 10.47NGNEE65 pKa = 3.81IFIGSDD71 pKa = 3.54DD72 pKa = 3.89GFLYY76 pKa = 10.86

Molecular weight: 8.21 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A6A8FLY4|A0A6A8FLY4_9ARCH Cation diffusion facilitator family transporter (Fragment) OS=Nitrosopumilales archaeon OX=2026768 GN=FIO04_07455 PE=4 SV=1
MM1 pKa = 6.95STDD4 pKa = 3.08IAEE7 pKa = 4.17VTQYY11 pKa = 10.63KK12 pKa = 9.51WGVAHH17 pKa = 7.5IFSSYY22 pKa = 11.16NNTLVHH28 pKa = 5.86ITDD31 pKa = 3.22VSGAEE36 pKa = 4.02TVSFSSGGRR45 pKa = 11.84HH46 pKa = 4.03VTADD50 pKa = 3.6RR51 pKa = 11.84YY52 pKa = 10.47EE53 pKa = 4.3SSPYY57 pKa = 10.94AAMKK61 pKa = 10.23SAVAAADD68 pKa = 3.44AAKK71 pKa = 9.93TKK73 pKa = 10.54GINALHH79 pKa = 5.96IRR81 pKa = 11.84VRR83 pKa = 11.84AVGGVGPRR91 pKa = 11.84IPGPGAQAAIRR102 pKa = 11.84ALARR106 pKa = 11.84AGFRR110 pKa = 11.84IGRR113 pKa = 11.84IDD115 pKa = 4.13DD116 pKa = 3.86VTPVPHH122 pKa = 6.81DD123 pKa = 3.57TTRR126 pKa = 11.84KK127 pKa = 7.63PGGRR131 pKa = 11.84RR132 pKa = 11.84GRR134 pKa = 11.84RR135 pKa = 11.84VV136 pKa = 2.81

Molecular weight: 14.35 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

1495

0

1495

335054

35

1272

224.1

25.01

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

7.019 ± 0.058

1.032 ± 0.025

5.276 ± 0.045

6.069 ± 0.073

3.89 ± 0.043

6.768 ± 0.061

2.051 ± 0.03

8.631 ± 0.059

6.939 ± 0.064

9.077 ± 0.067

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.578 ± 0.03

4.979 ± 0.047

3.809 ± 0.039

3.122 ± 0.037

5.078 ± 0.05

7.332 ± 0.056

5.493 ± 0.054

6.744 ± 0.049

0.853 ± 0.022

3.259 ± 0.038
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski