Amino acid dipepetide frequency for Lactobacillus saerimneri 30a

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10-3.

For more information see sequence space article on Wikipedia.

AlaCysAspGluPheGlyHisIleLysLeuMetAsnProGlnArgSerThrValTrpTyrXaa
Ala
7.847AlaAla: 7.847 ± 0.196
0.5AlaCys: 0.5 ± 0.032
5.327AlaAsp: 5.327 ± 0.114
5.019AlaGlu: 5.019 ± 0.107
3.264AlaPhe: 3.264 ± 0.094
6.361AlaGly: 6.361 ± 0.149
1.963AlaHis: 1.963 ± 0.074
5.471AlaIle: 5.471 ± 0.132
5.039AlaLys: 5.039 ± 0.118
8.383AlaLeu: 8.383 ± 0.176
2.27AlaMet: 2.27 ± 0.073
3.936AlaAsn: 3.936 ± 0.116
2.743AlaPro: 2.743 ± 0.079
5.045AlaGln: 5.045 ± 0.183
3.478AlaArg: 3.478 ± 0.105
4.178AlaSer: 4.178 ± 0.097
5.922AlaThr: 5.922 ± 0.14
6.416AlaVal: 6.416 ± 0.138
0.846AlaTrp: 0.846 ± 0.04
2.8AlaTyr: 2.8 ± 0.082
0.0AlaXaa: 0.0 ± 0.0
Cys
0.432CysAla: 0.432 ± 0.031
0.059CysCys: 0.059 ± 0.012
0.295CysAsp: 0.295 ± 0.027
0.252CysGlu: 0.252 ± 0.023
0.235CysPhe: 0.235 ± 0.022
0.549CysGly: 0.549 ± 0.032
0.229CysHis: 0.229 ± 0.026
0.301CysIle: 0.301 ± 0.026
0.218CysLys: 0.218 ± 0.025
0.591CysLeu: 0.591 ± 0.034
0.106CysMet: 0.106 ± 0.015
0.167CysAsn: 0.167 ± 0.02
0.284CysPro: 0.284 ± 0.029
0.295CysGln: 0.295 ± 0.026
0.276CysArg: 0.276 ± 0.027
0.382CysSer: 0.382 ± 0.028
0.261CysThr: 0.261 ± 0.025
0.373CysVal: 0.373 ± 0.033
0.068CysTrp: 0.068 ± 0.012
0.246CysTyr: 0.246 ± 0.025
0.0CysXaa: 0.0 ± 0.0
Asp
4.106AspAla: 4.106 ± 0.11
0.273AspCys: 0.273 ± 0.025
3.324AspAsp: 3.324 ± 0.116
4.034AspGlu: 4.034 ± 0.106
2.79AspPhe: 2.79 ± 0.077
3.729AspGly: 3.729 ± 0.112
1.376AspHis: 1.376 ± 0.055
3.705AspIle: 3.705 ± 0.104
3.307AspLys: 3.307 ± 0.092
5.611AspLeu: 5.611 ± 0.122
1.38AspMet: 1.38 ± 0.05
2.419AspAsn: 2.419 ± 0.079
2.618AspPro: 2.618 ± 0.07
3.398AspGln: 3.398 ± 0.11
2.55AspArg: 2.55 ± 0.069
2.622AspSer: 2.622 ± 0.072
2.794AspThr: 2.794 ± 0.104
3.807AspVal: 3.807 ± 0.102
0.627AspTrp: 0.627 ± 0.042
2.558AspTyr: 2.558 ± 0.063
0.0AspXaa: 0.0 ± 0.0
Glu
4.568GluAla: 4.568 ± 0.12
0.233GluCys: 0.233 ± 0.023
2.726GluAsp: 2.726 ± 0.088
3.597GluGlu: 3.597 ± 0.106
2.185GluPhe: 2.185 ± 0.074
2.76GluGly: 2.76 ± 0.081
1.28GluHis: 1.28 ± 0.046
4.032GluIle: 4.032 ± 0.101
4.233GluLys: 4.233 ± 0.105
5.963GluLeu: 5.963 ± 0.132
1.87GluMet: 1.87 ± 0.066
2.571GluAsn: 2.571 ± 0.076
1.719GluPro: 1.719 ± 0.067
3.305GluGln: 3.305 ± 0.1
3.031GluArg: 3.031 ± 0.103
2.122GluSer: 2.122 ± 0.067
2.711GluThr: 2.711 ± 0.076
4.136GluVal: 4.136 ± 0.105
0.541GluTrp: 0.541 ± 0.034
2.166GluTyr: 2.166 ± 0.07
0.0GluXaa: 0.0 ± 0.0
Phe
3.676PheAla: 3.676 ± 0.105
0.309PheCys: 0.309 ± 0.029
2.597PheAsp: 2.597 ± 0.068
1.912PheGlu: 1.912 ± 0.07
1.976PhePhe: 1.976 ± 0.085
3.038PheGly: 3.038 ± 0.082
0.774PheHis: 0.774 ± 0.047
2.957PheIle: 2.957 ± 0.091
2.291PheLys: 2.291 ± 0.083
3.432PheLeu: 3.432 ± 0.104
1.13PheMet: 1.13 ± 0.049
2.005PheAsn: 2.005 ± 0.066
1.437PhePro: 1.437 ± 0.057
1.458PheGln: 1.458 ± 0.058
1.399PheArg: 1.399 ± 0.053
2.446PheSer: 2.446 ± 0.088
2.756PheThr: 2.756 ± 0.085
2.902PheVal: 2.902 ± 0.099
0.466PheTrp: 0.466 ± 0.029
1.581PheTyr: 1.581 ± 0.054
0.0PheXaa: 0.0 ± 0.0
Gly
4.935GlyAla: 4.935 ± 0.141
0.415GlyCys: 0.415 ± 0.035
3.498GlyAsp: 3.498 ± 0.093
3.421GlyGlu: 3.421 ± 0.08
2.987GlyPhe: 2.987 ± 0.083
4.358GlyGly: 4.358 ± 0.115
1.624GlyHis: 1.624 ± 0.068
5.357GlyIle: 5.357 ± 0.133
4.731GlyLys: 4.731 ± 0.115
6.391GlyLeu: 6.391 ± 0.126
2.094GlyMet: 2.094 ± 0.068
2.73GlyAsn: 2.73 ± 0.085
1.692GlyPro: 1.692 ± 0.059
3.379GlyGln: 3.379 ± 0.095
3.05GlyArg: 3.05 ± 0.085
3.733GlySer: 3.733 ± 0.1
4.201GlyThr: 4.201 ± 0.117
5.051GlyVal: 5.051 ± 0.116
0.878GlyTrp: 0.878 ± 0.054
2.881GlyTyr: 2.881 ± 0.09
0.0GlyXaa: 0.0 ± 0.0
His
1.518HisAla: 1.518 ± 0.062
0.121HisCys: 0.121 ± 0.017
1.31HisAsp: 1.31 ± 0.055
1.208HisGlu: 1.208 ± 0.051
1.064HisPhe: 1.064 ± 0.05
1.677HisGly: 1.677 ± 0.051
0.716HisHis: 0.716 ± 0.041
1.261HisIle: 1.261 ± 0.052
1.147HisLys: 1.147 ± 0.051
2.287HisLeu: 2.287 ± 0.078
0.498HisMet: 0.498 ± 0.032
1.022HisAsn: 1.022 ± 0.046
1.117HisPro: 1.117 ± 0.048
1.293HisGln: 1.293 ± 0.056
1.079HisArg: 1.079 ± 0.053
0.99HisSer: 0.99 ± 0.047
1.109HisThr: 1.109 ± 0.054
1.382HisVal: 1.382 ± 0.059
0.303HisTrp: 0.303 ± 0.027
0.977HisTyr: 0.977 ± 0.046
0.0HisXaa: 0.0 ± 0.0
Ile
6.34IleAla: 6.34 ± 0.145
0.536IleCys: 0.536 ± 0.038
3.972IleAsp: 3.972 ± 0.091
3.436IleGlu: 3.436 ± 0.094
2.65IlePhe: 2.65 ± 0.094
4.723IleGly: 4.723 ± 0.141
1.28IleHis: 1.28 ± 0.046
4.708IleIle: 4.708 ± 0.128
4.125IleLys: 4.125 ± 0.088
5.961IleLeu: 5.961 ± 0.141
1.639IleMet: 1.639 ± 0.069
3.222IleAsn: 3.222 ± 0.092
2.866IlePro: 2.866 ± 0.078
2.448IleGln: 2.448 ± 0.083
2.66IleArg: 2.66 ± 0.079
3.608IleSer: 3.608 ± 0.09
4.619IleThr: 4.619 ± 0.098
4.869IleVal: 4.869 ± 0.122
0.568IleTrp: 0.568 ± 0.041
2.232IleTyr: 2.232 ± 0.075
0.0IleXaa: 0.0 ± 0.0
Lys
4.507LysAla: 4.507 ± 0.122
0.197LysCys: 0.197 ± 0.022
3.43LysAsp: 3.43 ± 0.101
3.919LysGlu: 3.919 ± 0.098
1.978LysPhe: 1.978 ± 0.075
3.194LysGly: 3.194 ± 0.082
1.255LysHis: 1.255 ± 0.052
4.199LysIle: 4.199 ± 0.104
4.869LysLys: 4.869 ± 0.134
5.39LysLeu: 5.39 ± 0.116
2.118LysMet: 2.118 ± 0.077
2.976LysAsn: 2.976 ± 0.086
1.94LysPro: 1.94 ± 0.068
3.716LysGln: 3.716 ± 0.096
3.29LysArg: 3.29 ± 0.099
2.639LysSer: 2.639 ± 0.097
3.642LysThr: 3.642 ± 0.091
4.432LysVal: 4.432 ± 0.1
0.627LysTrp: 0.627 ± 0.036
2.472LysTyr: 2.472 ± 0.078
0.0LysXaa: 0.0 ± 0.0
Leu
9.763LeuAla: 9.763 ± 0.191
0.591LeuCys: 0.591 ± 0.035
5.545LeuAsp: 5.545 ± 0.122
4.685LeuGlu: 4.685 ± 0.121
3.962LeuPhe: 3.962 ± 0.114
6.792LeuGly: 6.792 ± 0.147
2.124LeuHis: 2.124 ± 0.069
5.92LeuIle: 5.92 ± 0.14
5.261LeuLys: 5.261 ± 0.122
9.532LeuLeu: 9.532 ± 0.208
2.27LeuMet: 2.27 ± 0.085
4.18LeuAsn: 4.18 ± 0.094
4.246LeuPro: 4.246 ± 0.095
4.53LeuGln: 4.53 ± 0.122
4.543LeuArg: 4.543 ± 0.108
5.174LeuSer: 5.174 ± 0.112
6.874LeuThr: 6.874 ± 0.144
7.3LeuVal: 7.3 ± 0.139
0.878LeuTrp: 0.878 ± 0.051
2.849LeuTyr: 2.849 ± 0.09
0.0LeuXaa: 0.0 ± 0.0
Met
2.463MetAla: 2.463 ± 0.076
0.146MetCys: 0.146 ± 0.02
1.471MetAsp: 1.471 ± 0.062
1.429MetGlu: 1.429 ± 0.063
0.842MetPhe: 0.842 ± 0.04
1.861MetGly: 1.861 ± 0.073
0.432MetHis: 0.432 ± 0.026
1.891MetIle: 1.891 ± 0.064
1.764MetLys: 1.764 ± 0.061
2.516MetLeu: 2.516 ± 0.082
0.833MetMet: 0.833 ± 0.043
1.219MetAsn: 1.219 ± 0.05
1.126MetPro: 1.126 ± 0.047
1.257MetGln: 1.257 ± 0.065
1.244MetArg: 1.244 ± 0.057
1.321MetSer: 1.321 ± 0.059
1.844MetThr: 1.844 ± 0.06
2.029MetVal: 2.029 ± 0.065
0.182MetTrp: 0.182 ± 0.02
0.678MetTyr: 0.678 ± 0.033
0.0MetXaa: 0.0 ± 0.0
Asn
3.258AsnAla: 3.258 ± 0.092
0.244AsnCys: 0.244 ± 0.022
2.779AsnAsp: 2.779 ± 0.089
2.527AsnGlu: 2.527 ± 0.081
1.823AsnPhe: 1.823 ± 0.066
3.224AsnGly: 3.224 ± 0.102
1.032AsnHis: 1.032 ± 0.046
3.025AsnIle: 3.025 ± 0.083
2.904AsnLys: 2.904 ± 0.074
4.023AsnLeu: 4.023 ± 0.098
1.289AsnMet: 1.289 ± 0.045
2.416AsnAsn: 2.416 ± 0.076
1.918AsnPro: 1.918 ± 0.069
2.13AsnGln: 2.13 ± 0.077
1.91AsnArg: 1.91 ± 0.073
2.357AsnSer: 2.357 ± 0.07
2.306AsnThr: 2.306 ± 0.075
2.934AsnVal: 2.934 ± 0.088
0.566AsnTrp: 0.566 ± 0.037
1.851AsnTyr: 1.851 ± 0.065
0.0AsnXaa: 0.0 ± 0.0
Pro
3.606ProAla: 3.606 ± 0.097
0.159ProCys: 0.159 ± 0.02
2.565ProAsp: 2.565 ± 0.08
2.73ProGlu: 2.73 ± 0.073
1.564ProPhe: 1.564 ± 0.059
2.539ProGly: 2.539 ± 0.075
0.886ProHis: 0.886 ± 0.044
2.054ProIle: 2.054 ± 0.065
1.891ProLys: 1.891 ± 0.07
3.574ProLeu: 3.574 ± 0.081
0.757ProMet: 0.757 ± 0.044
1.628ProAsn: 1.628 ± 0.054
0.846ProPro: 0.846 ± 0.044
2.215ProGln: 2.215 ± 0.073
1.414ProArg: 1.414 ± 0.056
1.787ProSer: 1.787 ± 0.057
2.529ProThr: 2.529 ± 0.069
3.033ProVal: 3.033 ± 0.085
0.369ProTrp: 0.369 ± 0.036
1.365ProTyr: 1.365 ± 0.058
0.0ProXaa: 0.0 ± 0.0
Gln
5.607GlnAla: 5.607 ± 0.198
0.18GlnCys: 0.18 ± 0.021
2.48GlnAsp: 2.48 ± 0.074
3.135GlnGlu: 3.135 ± 0.091
1.554GlnPhe: 1.554 ± 0.057
2.955GlnGly: 2.955 ± 0.089
1.03GlnHis: 1.03 ± 0.042
3.027GlnIle: 3.027 ± 0.092
3.385GlnLys: 3.385 ± 0.094
5.149GlnLeu: 5.149 ± 0.126
1.435GlnMet: 1.435 ± 0.064
1.918GlnAsn: 1.918 ± 0.07
1.918GlnPro: 1.918 ± 0.08
2.902GlnGln: 2.902 ± 0.108
2.518GlnArg: 2.518 ± 0.085
2.014GlnSer: 2.014 ± 0.076
3.328GlnThr: 3.328 ± 0.118
4.208GlnVal: 4.208 ± 0.103
0.566GlnTrp: 0.566 ± 0.036
1.736GlnTyr: 1.736 ± 0.067
0.0GlnXaa: 0.0 ± 0.0
Arg
3.29ArgAla: 3.29 ± 0.089
0.201ArgCys: 0.201 ± 0.021
2.321ArgAsp: 2.321 ± 0.078
2.749ArgGlu: 2.749 ± 0.078
1.94ArgPhe: 1.94 ± 0.061
2.565ArgGly: 2.565 ± 0.083
1.117ArgHis: 1.117 ± 0.045
2.961ArgIle: 2.961 ± 0.082
2.974ArgLys: 2.974 ± 0.092
4.721ArgLeu: 4.721 ± 0.121
1.265ArgMet: 1.265 ± 0.049
1.956ArgAsn: 1.956 ± 0.063
1.677ArgPro: 1.677 ± 0.064
2.749ArgGln: 2.749 ± 0.088
2.563ArgArg: 2.563 ± 0.072
2.075ArgSer: 2.075 ± 0.062
2.433ArgThr: 2.433 ± 0.059
2.991ArgVal: 2.991 ± 0.09
0.445ArgTrp: 0.445 ± 0.031
1.88ArgTyr: 1.88 ± 0.067
0.0ArgXaa: 0.0 ± 0.0
Ser
4.087SerAla: 4.087 ± 0.09
0.24SerCys: 0.24 ± 0.025
2.859SerAsp: 2.859 ± 0.081
2.597SerGlu: 2.597 ± 0.089
2.501SerPhe: 2.501 ± 0.09
3.934SerGly: 3.934 ± 0.107
1.132SerHis: 1.132 ± 0.047
3.071SerIle: 3.071 ± 0.099
2.919SerLys: 2.919 ± 0.08
5.348SerLeu: 5.348 ± 0.126
1.229SerMet: 1.229 ± 0.054
2.211SerAsn: 2.211 ± 0.073
1.859SerPro: 1.859 ± 0.064
2.622SerGln: 2.622 ± 0.079
2.209SerArg: 2.209 ± 0.075
3.483SerSer: 3.483 ± 0.202
2.777SerThr: 2.777 ± 0.083
3.561SerVal: 3.561 ± 0.098
0.613SerTrp: 0.613 ± 0.035
1.81SerTyr: 1.81 ± 0.068
0.0SerXaa: 0.0 ± 0.0
Thr
5.833ThrAla: 5.833 ± 0.148
0.326ThrCys: 0.326 ± 0.027
3.625ThrAsp: 3.625 ± 0.098
3.069ThrGlu: 3.069 ± 0.076
2.355ThrPhe: 2.355 ± 0.073
4.68ThrGly: 4.68 ± 0.112
1.134ThrHis: 1.134 ± 0.05
4.396ThrIle: 4.396 ± 0.119
3.468ThrLys: 3.468 ± 0.098
5.774ThrLeu: 5.774 ± 0.109
1.513ThrMet: 1.513 ± 0.048
2.83ThrAsn: 2.83 ± 0.086
2.953ThrPro: 2.953 ± 0.09
2.565ThrGln: 2.565 ± 0.09
2.171ThrArg: 2.171 ± 0.068
3.487ThrSer: 3.487 ± 0.113
5.079ThrThr: 5.079 ± 0.162
5.212ThrVal: 5.212 ± 0.131
0.636ThrTrp: 0.636 ± 0.041
2.361ThrTyr: 2.361 ± 0.084
0.0ThrXaa: 0.0 ± 0.0
Val
7.567ValAla: 7.567 ± 0.143
0.534ValCys: 0.534 ± 0.034
4.208ValAsp: 4.208 ± 0.096
3.767ValGlu: 3.767 ± 0.098
2.62ValPhe: 2.62 ± 0.07
5.019ValGly: 5.019 ± 0.113
1.371ValHis: 1.371 ± 0.058
5.299ValIle: 5.299 ± 0.123
4.271ValLys: 4.271 ± 0.104
6.845ValLeu: 6.845 ± 0.124
1.791ValMet: 1.791 ± 0.063
3.139ValAsn: 3.139 ± 0.085
2.868ValPro: 2.868 ± 0.074
2.732ValGln: 2.732 ± 0.078
2.936ValArg: 2.936 ± 0.096
4.038ValSer: 4.038 ± 0.101
5.596ValThr: 5.596 ± 0.143
5.98ValVal: 5.98 ± 0.123
0.727ValTrp: 0.727 ± 0.044
2.469ValTyr: 2.469 ± 0.081
0.0ValXaa: 0.0 ± 0.0
Trp
0.725TrpAla: 0.725 ± 0.038
0.064TrpCys: 0.064 ± 0.011
0.566TrpAsp: 0.566 ± 0.038
0.479TrpGlu: 0.479 ± 0.033
0.464TrpPhe: 0.464 ± 0.035
0.789TrpGly: 0.789 ± 0.047
0.314TrpHis: 0.314 ± 0.025
0.644TrpIle: 0.644 ± 0.04
0.488TrpLys: 0.488 ± 0.033
1.323TrpLeu: 1.323 ± 0.064
0.273TrpMet: 0.273 ± 0.025
0.471TrpAsn: 0.471 ± 0.036
0.276TrpPro: 0.276 ± 0.025
0.742TrpGln: 0.742 ± 0.043
0.534TrpArg: 0.534 ± 0.038
0.53TrpSer: 0.53 ± 0.031
0.587TrpThr: 0.587 ± 0.034
0.695TrpVal: 0.695 ± 0.043
0.167TrpTrp: 0.167 ± 0.023
0.435TrpTyr: 0.435 ± 0.056
0.0TrpXaa: 0.0 ± 0.0
Tyr
2.684TyrAla: 2.684 ± 0.079
0.288TyrCys: 0.288 ± 0.027
2.13TyrAsp: 2.13 ± 0.065
1.783TyrGlu: 1.783 ± 0.066
1.789TyrPhe: 1.789 ± 0.061
2.658TyrGly: 2.658 ± 0.076
1.009TyrHis: 1.009 ± 0.05
2.181TyrIle: 2.181 ± 0.069
1.505TyrLys: 1.505 ± 0.069
4.206TyrLeu: 4.206 ± 0.113
0.784TyrMet: 0.784 ± 0.044
1.501TyrAsn: 1.501 ± 0.062
1.41TyrPro: 1.41 ± 0.054
2.264TyrGln: 2.264 ± 0.081
2.02TyrArg: 2.02 ± 0.073
2.077TyrSer: 2.077 ± 0.064
2.101TyrThr: 2.101 ± 0.088
2.467TyrVal: 2.467 ± 0.067
0.492TyrTrp: 0.492 ± 0.038
1.785TyrTyr: 1.785 ± 0.084
0.0TyrXaa: 0.0 ± 0.0
Xaa
0.0XaaAla: 0.0 ± 0.0
0.0XaaCys: 0.0 ± 0.0
0.0XaaAsp: 0.0 ± 0.0
0.0XaaGlu: 0.0 ± 0.0
0.0XaaPhe: 0.0 ± 0.0
0.0XaaGly: 0.0 ± 0.0
0.0XaaHis: 0.0 ± 0.0
0.0XaaIle: 0.0 ± 0.0
0.0XaaLys: 0.0 ± 0.0
0.0XaaLeu: 0.0 ± 0.0
0.0XaaMet: 0.0 ± 0.0
0.0XaaAsn: 0.0 ± 0.0
0.0XaaPro: 0.0 ± 0.0
0.0XaaGln: 0.0 ± 0.0
0.0XaaArg: 0.0 ± 0.0
0.0XaaSer: 0.0 ± 0.0
0.0XaaThr: 0.0 ± 0.0
0.0XaaVal: 0.0 ± 0.0
0.0XaaTrp: 0.0 ± 0.0
0.0XaaTyr: 0.0 ± 0.0
0.0XaaXaa: 0.0 ± 0.0
Statistics based on 1519 proteins (471763 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski