Amino acid dipepetide frequency for Clostridium sp. CAG:149

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10^-3.

For more information see sequence space article on Wikipedia.

	Ala	Cys	Asp	Glu	Phe	Gly	His	Ile	Lys	Leu	Met	Asn	Pro	Gln	Arg	Ser	Thr	Val	Trp	Tyr	Xaa
Ala	9.669AlaAla: 9.669 ± 0.166	1.324AlaCys: 1.324 ± 0.043	4.701AlaAsp: 4.701 ± 0.086	6.49AlaGlu: 6.49 ± 0.108	3.4AlaPhe: 3.4 ± 0.07	8.155AlaGly: 8.155 ± 0.126	1.092AlaHis: 1.092 ± 0.037	4.244AlaIle: 4.244 ± 0.079	4.122AlaLys: 4.122 ± 0.095	7.665AlaLeu: 7.665 ± 0.104	2.479AlaMet: 2.479 ± 0.052	2.022AlaAsn: 2.022 ± 0.048	2.219AlaPro: 2.219 ± 0.059	2.384AlaGln: 2.384 ± 0.06	3.89AlaArg: 3.89 ± 0.075	4.4AlaSer: 4.4 ± 0.078	2.625AlaThr: 2.625 ± 0.062	7.869AlaVal: 7.869 ± 0.119	0.682AlaTrp: 0.682 ± 0.029	2.578AlaTyr: 2.578 ± 0.061	0.004AlaXaa: 0.004 ± 0.002
Cys	1.146CysAla: 1.146 ± 0.036	0.319CysCys: 0.319 ± 0.021	0.743CysAsp: 0.743 ± 0.031	0.898CysGlu: 0.898 ± 0.034	0.662CysPhe: 0.662 ± 0.03	1.523CysGly: 1.523 ± 0.05	0.345CysHis: 0.345 ± 0.024	1.026CysIle: 1.026 ± 0.032	0.45CysLys: 0.45 ± 0.026	1.425CysLeu: 1.425 ± 0.043	0.469CysMet: 0.469 ± 0.023	0.39CysAsn: 0.39 ± 0.023	0.759CysPro: 0.759 ± 0.037	0.455CysGln: 0.455 ± 0.024	1.201CysArg: 1.201 ± 0.045	1.013CysSer: 1.013 ± 0.032	0.692CysThr: 0.692 ± 0.031	0.975CysVal: 0.975 ± 0.035	0.101CysTrp: 0.101 ± 0.01	0.518CysTyr: 0.518 ± 0.026	0.0CysXaa: 0.0 ± 0.0
Asp	3.814AspAla: 3.814 ± 0.078	0.864AspCys: 0.864 ± 0.032	2.387AspAsp: 2.387 ± 0.072	4.381AspGlu: 4.381 ± 0.085	2.308AspPhe: 2.308 ± 0.06	4.837AspGly: 4.837 ± 0.108	0.797AspHis: 0.797 ± 0.037	3.713AspIle: 3.713 ± 0.072	2.49AspLys: 2.49 ± 0.058	4.226AspLeu: 4.226 ± 0.071	1.788AspMet: 1.788 ± 0.049	1.621AspAsn: 1.621 ± 0.047	1.846AspPro: 1.846 ± 0.064	1.294AspGln: 1.294 ± 0.04	3.398AspArg: 3.398 ± 0.068	3.331AspSer: 3.331 ± 0.072	2.801AspThr: 2.801 ± 0.061	3.286AspVal: 3.286 ± 0.077	0.571AspTrp: 0.571 ± 0.031	2.377AspTyr: 2.377 ± 0.057	0.001AspXaa: 0.001 ± 0.001
Glu	6.839GluAla: 6.839 ± 0.092	0.745GluCys: 0.745 ± 0.03	4.149GluAsp: 4.149 ± 0.08	8.973GluGlu: 8.973 ± 0.138	2.636GluPhe: 2.636 ± 0.058	5.532GluGly: 5.532 ± 0.1	1.392GluHis: 1.392 ± 0.044	5.598GluIle: 5.598 ± 0.097	6.915GluLys: 6.915 ± 0.111	7.542GluLeu: 7.542 ± 0.109	2.593GluMet: 2.593 ± 0.062	3.89GluAsn: 3.89 ± 0.076	2.422GluPro: 2.422 ± 0.063	3.062GluGln: 3.062 ± 0.059	4.885GluArg: 4.885 ± 0.092	3.742GluSer: 3.742 ± 0.078	4.155GluThr: 4.155 ± 0.079	4.195GluVal: 4.195 ± 0.082	0.667GluTrp: 0.667 ± 0.033	2.88GluTyr: 2.88 ± 0.069	0.001GluXaa: 0.001 ± 0.001
Phe	3.029PheAla: 3.029 ± 0.066	0.842PheCys: 0.842 ± 0.035	2.325PheAsp: 2.325 ± 0.054	2.765PheGlu: 2.765 ± 0.061	1.852PhePhe: 1.852 ± 0.059	3.305PheGly: 3.305 ± 0.069	0.838PheHis: 0.838 ± 0.031	2.533PheIle: 2.533 ± 0.061	1.675PheLys: 1.675 ± 0.044	4.499PheLeu: 4.499 ± 0.089	1.166PheMet: 1.166 ± 0.04	1.277PheAsn: 1.277 ± 0.043	1.588PhePro: 1.588 ± 0.046	1.272PheGln: 1.272 ± 0.043	2.084PheArg: 2.084 ± 0.053	3.027PheSer: 3.027 ± 0.063	2.226PheThr: 2.226 ± 0.058	2.649PheVal: 2.649 ± 0.066	0.424PheTrp: 0.424 ± 0.025	1.669PheTyr: 1.669 ± 0.052	0.0PheXaa: 0.0 ± 0.0
Gly	5.902GlyAla: 5.902 ± 0.091	1.321GlyCys: 1.321 ± 0.043	3.557GlyAsp: 3.557 ± 0.083	6.158GlyGlu: 6.158 ± 0.107	3.392GlyPhe: 3.392 ± 0.071	5.738GlyGly: 5.738 ± 0.098	1.338GlyHis: 1.338 ± 0.049	6.323GlyIle: 6.323 ± 0.096	5.35GlyLys: 5.35 ± 0.084	6.856GlyLeu: 6.856 ± 0.094	2.675GlyMet: 2.675 ± 0.062	2.947GlyAsn: 2.947 ± 0.083	1.747GlyPro: 1.747 ± 0.045	2.625GlyGln: 2.625 ± 0.065	4.608GlyArg: 4.608 ± 0.08	4.911GlySer: 4.911 ± 0.107	4.721GlyThr: 4.721 ± 0.088	5.005GlyVal: 5.005 ± 0.078	0.937GlyTrp: 0.937 ± 0.051	3.12GlyTyr: 3.12 ± 0.059	0.009GlyXaa: 0.009 ± 0.003
His	1.063HisAla: 1.063 ± 0.034	0.33HisCys: 0.33 ± 0.022	0.822HisAsp: 0.822 ± 0.042	1.04HisGlu: 1.04 ± 0.035	0.817HisPhe: 0.817 ± 0.031	1.266HisGly: 1.266 ± 0.042	0.373HisHis: 0.373 ± 0.028	1.233HisIle: 1.233 ± 0.045	0.672HisLys: 0.672 ± 0.032	1.505HisLeu: 1.505 ± 0.044	0.51HisMet: 0.51 ± 0.023	0.551HisAsn: 0.551 ± 0.029	0.885HisPro: 0.885 ± 0.031	0.476HisGln: 0.476 ± 0.025	0.978HisArg: 0.978 ± 0.037	1.038HisSer: 1.038 ± 0.036	0.862HisThr: 0.862 ± 0.033	1.127HisVal: 1.127 ± 0.042	0.173HisTrp: 0.173 ± 0.014	0.658HisTyr: 0.658 ± 0.032	0.0HisXaa: 0.0 ± 0.0
Ile	5.233IleAla: 5.233 ± 0.093	1.28IleCys: 1.28 ± 0.038	3.424IleAsp: 3.424 ± 0.07	4.484IleGlu: 4.484 ± 0.079	2.709IlePhe: 2.709 ± 0.064	4.987IleGly: 4.987 ± 0.084	1.208IleHis: 1.208 ± 0.041	4.088IleIle: 4.088 ± 0.09	3.025IleLys: 3.025 ± 0.068	6.822IleLeu: 6.822 ± 0.118	1.669IleMet: 1.669 ± 0.047	2.241IleAsn: 2.241 ± 0.059	3.16IlePro: 3.16 ± 0.067	2.113IleGln: 2.113 ± 0.052	4.062IleArg: 4.062 ± 0.069	4.401IleSer: 4.401 ± 0.077	3.459IleThr: 3.459 ± 0.072	4.502IleVal: 4.502 ± 0.071	0.57IleTrp: 0.57 ± 0.029	2.358IleTyr: 2.358 ± 0.051	0.001IleXaa: 0.001 ± 0.001
Lys	4.862LysAla: 4.862 ± 0.073	0.56LysCys: 0.56 ± 0.028	3.042LysAsp: 3.042 ± 0.062	6.025LysGlu: 6.025 ± 0.11	1.58LysPhe: 1.58 ± 0.044	4.06LysGly: 4.06 ± 0.069	0.837LysHis: 0.837 ± 0.031	3.892LysIle: 3.892 ± 0.067	5.367LysLys: 5.367 ± 0.097	4.551LysLeu: 4.551 ± 0.078	1.842LysMet: 1.842 ± 0.049	2.912LysAsn: 2.912 ± 0.058	1.822LysPro: 1.822 ± 0.051	1.912LysGln: 1.912 ± 0.056	3.534LysArg: 3.534 ± 0.084	2.807LysSer: 2.807 ± 0.062	3.422LysThr: 3.422 ± 0.071	3.287LysVal: 3.287 ± 0.072	0.465LysTrp: 0.465 ± 0.027	2.105LysTyr: 2.105 ± 0.049	0.0LysXaa: 0.0 ± 0.0
Leu	7.66LeuAla: 7.66 ± 0.096	1.632LeuCys: 1.632 ± 0.045	4.745LeuAsp: 4.745 ± 0.089	6.475LeuGlu: 6.475 ± 0.109	4.149LeuPhe: 4.149 ± 0.089	6.511LeuGly: 6.511 ± 0.111	1.555LeuHis: 1.555 ± 0.051	5.815LeuIle: 5.815 ± 0.11	5.869LeuLys: 5.869 ± 0.085	9.48LeuLeu: 9.48 ± 0.14	2.751LeuMet: 2.751 ± 0.067	3.496LeuAsn: 3.496 ± 0.074	3.923LeuPro: 3.923 ± 0.079	2.424LeuGln: 2.424 ± 0.056	4.826LeuArg: 4.826 ± 0.09	6.934LeuSer: 6.934 ± 0.107	5.138LeuThr: 5.138 ± 0.072	5.473LeuVal: 5.473 ± 0.096	0.837LeuTrp: 0.837 ± 0.034	3.168LeuTyr: 3.168 ± 0.064	0.002LeuXaa: 0.002 ± 0.002
Met	2.889MetAla: 2.889 ± 0.059	0.365MetCys: 0.365 ± 0.023	1.879MetAsp: 1.879 ± 0.051	3.018MetGlu: 3.018 ± 0.058	1.025MetPhe: 1.025 ± 0.039	2.388MetGly: 2.388 ± 0.056	0.393MetHis: 0.393 ± 0.023	1.992MetIle: 1.992 ± 0.053	2.419MetLys: 2.419 ± 0.058	2.495MetLeu: 2.495 ± 0.056	0.908MetMet: 0.908 ± 0.04	1.377MetAsn: 1.377 ± 0.046	1.092MetPro: 1.092 ± 0.042	0.875MetGln: 0.875 ± 0.034	1.515MetArg: 1.515 ± 0.039	1.571MetSer: 1.571 ± 0.045	1.818MetThr: 1.818 ± 0.05	1.881MetVal: 1.881 ± 0.051	0.188MetTrp: 0.188 ± 0.016	0.842MetTyr: 0.842 ± 0.037	0.0MetXaa: 0.0 ± 0.0
Asn	2.639AsnAla: 2.639 ± 0.062	0.515AsnCys: 0.515 ± 0.026	1.523AsnAsp: 1.523 ± 0.046	2.348AsnGlu: 2.348 ± 0.048	1.382AsnPhe: 1.382 ± 0.041	3.22AsnGly: 3.22 ± 0.086	0.663AsnHis: 0.663 ± 0.03	2.623AsnIle: 2.623 ± 0.064	1.52AsnLys: 1.52 ± 0.049	3.418AsnLeu: 3.418 ± 0.075	1.215AsnMet: 1.215 ± 0.04	1.173AsnAsn: 1.173 ± 0.05	1.959AsnPro: 1.959 ± 0.049	1.311AsnGln: 1.311 ± 0.042	2.262AsnArg: 2.262 ± 0.055	2.055AsnSer: 2.055 ± 0.054	1.879AsnThr: 1.879 ± 0.052	2.393AsnVal: 2.393 ± 0.051	0.383AsnTrp: 0.383 ± 0.028	1.435AsnTyr: 1.435 ± 0.04	0.0AsnXaa: 0.0 ± 0.0
Pro	3.193ProAla: 3.193 ± 0.063	0.494ProCys: 0.494 ± 0.026	2.322ProAsp: 2.322 ± 0.057	3.966ProGlu: 3.966 ± 0.096	1.707ProPhe: 1.707 ± 0.046	3.145ProGly: 3.145 ± 0.069	0.571ProHis: 0.571 ± 0.026	1.841ProIle: 1.841 ± 0.051	1.684ProLys: 1.684 ± 0.049	3.079ProLeu: 3.079 ± 0.066	0.968ProMet: 0.968 ± 0.036	1.031ProAsn: 1.031 ± 0.035	0.905ProPro: 0.905 ± 0.04	1.025ProGln: 1.025 ± 0.036	1.207ProArg: 1.207 ± 0.041	2.095ProSer: 2.095 ± 0.055	1.247ProThr: 1.247 ± 0.046	3.383ProVal: 3.383 ± 0.07	0.352ProTrp: 0.352 ± 0.022	1.372ProTyr: 1.372 ± 0.039	0.0ProXaa: 0.0 ± 0.0
Gln	2.788GlnAla: 2.788 ± 0.064	0.308GlnCys: 0.308 ± 0.02	1.438GlnAsp: 1.438 ± 0.043	2.769GlnGlu: 2.769 ± 0.067	1.203GlnPhe: 1.203 ± 0.043	2.079GlnGly: 2.079 ± 0.056	0.41GlnHis: 0.41 ± 0.026	2.431GlnIle: 2.431 ± 0.056	2.328GlnLys: 2.328 ± 0.054	2.535GlnLeu: 2.535 ± 0.061	1.268GlnMet: 1.268 ± 0.043	1.367GlnAsn: 1.367 ± 0.045	0.959GlnPro: 0.959 ± 0.033	0.865GlnGln: 0.865 ± 0.036	1.432GlnArg: 1.432 ± 0.045	1.7GlnSer: 1.7 ± 0.051	1.746GlnThr: 1.746 ± 0.055	2.16GlnVal: 2.16 ± 0.055	0.318GlnTrp: 0.318 ± 0.02	1.18GlnTyr: 1.18 ± 0.033	0.0GlnXaa: 0.0 ± 0.0
Arg	3.896ArgAla: 3.896 ± 0.078	0.667ArgCys: 0.667 ± 0.03	2.7ArgAsp: 2.7 ± 0.063	6.481ArgGlu: 6.481 ± 0.111	2.192ArgPhe: 2.192 ± 0.052	3.622ArgGly: 3.622 ± 0.074	0.841ArgHis: 0.841 ± 0.033	3.824ArgIle: 3.824 ± 0.07	3.832ArgLys: 3.832 ± 0.072	5.41ArgLeu: 5.41 ± 0.105	1.943ArgMet: 1.943 ± 0.046	2.063ArgAsn: 2.063 ± 0.052	1.859ArgPro: 1.859 ± 0.054	2.256ArgGln: 2.256 ± 0.06	3.775ArgArg: 3.775 ± 0.086	2.484ArgSer: 2.484 ± 0.053	2.645ArgThr: 2.645 ± 0.053	3.212ArgVal: 3.212 ± 0.07	0.459ArgTrp: 0.459 ± 0.026	1.99ArgTyr: 1.99 ± 0.056	0.0ArgXaa: 0.0 ± 0.0
Ser	4.713SerAla: 4.713 ± 0.084	0.964SerCys: 0.964 ± 0.034	3.144SerAsp: 3.144 ± 0.073	4.27SerGlu: 4.27 ± 0.077	2.863SerPhe: 2.863 ± 0.061	5.768SerGly: 5.768 ± 0.113	1.06SerHis: 1.06 ± 0.038	3.604SerIle: 3.604 ± 0.066	2.248SerLys: 2.248 ± 0.061	5.693SerLeu: 5.693 ± 0.084	1.906SerMet: 1.906 ± 0.055	1.619SerAsn: 1.619 ± 0.048	2.079SerPro: 2.079 ± 0.054	2.058SerGln: 2.058 ± 0.053	3.734SerArg: 3.734 ± 0.073	3.83SerSer: 3.83 ± 0.094	2.457SerThr: 2.457 ± 0.059	4.448SerVal: 4.448 ± 0.081	0.609SerTrp: 0.609 ± 0.029	2.276SerTyr: 2.276 ± 0.053	0.0SerXaa: 0.0 ± 0.0
Thr	5.123ThrAla: 5.123 ± 0.085	0.635ThrCys: 0.635 ± 0.025	2.995ThrAsp: 2.995 ± 0.068	4.29ThrGlu: 4.29 ± 0.081	1.897ThrPhe: 1.897 ± 0.052	5.091ThrGly: 5.091 ± 0.082	0.722ThrHis: 0.722 ± 0.03	3.106ThrIle: 3.106 ± 0.072	2.444ThrLys: 2.444 ± 0.065	4.263ThrLeu: 4.263 ± 0.084	1.336ThrMet: 1.336 ± 0.04	1.513ThrAsn: 1.513 ± 0.048	2.073ThrPro: 2.073 ± 0.051	1.309ThrGln: 1.309 ± 0.044	2.101ThrArg: 2.101 ± 0.054	2.629ThrSer: 2.629 ± 0.062	2.205ThrThr: 2.205 ± 0.066	4.637ThrVal: 4.637 ± 0.083	0.441ThrTrp: 0.441 ± 0.026	1.718ThrTyr: 1.718 ± 0.061	0.002ThrXaa: 0.002 ± 0.002
Val	4.497ValAla: 4.497 ± 0.08	1.245ValCys: 1.245 ± 0.039	3.514ValAsp: 3.514 ± 0.066	4.484ValGlu: 4.484 ± 0.091	3.242ValPhe: 3.242 ± 0.071	4.395ValGly: 4.395 ± 0.086	1.09ValHis: 1.09 ± 0.042	5.018ValIle: 5.018 ± 0.088	4.18ValLys: 4.18 ± 0.08	6.813ValLeu: 6.813 ± 0.103	2.093ValMet: 2.093 ± 0.049	2.568ValAsn: 2.568 ± 0.057	2.665ValPro: 2.665 ± 0.057	1.982ValGln: 1.982 ± 0.052	3.686ValArg: 3.686 ± 0.069	4.733ValSer: 4.733 ± 0.081	4.029ValThr: 4.029 ± 0.074	4.392ValVal: 4.392 ± 0.085	0.616ValTrp: 0.616 ± 0.024	2.542ValTyr: 2.542 ± 0.06	0.001ValXaa: 0.001 ± 0.001
Trp	0.574TrpAla: 0.574 ± 0.028	0.127TrpCys: 0.127 ± 0.013	0.525TrpAsp: 0.525 ± 0.029	0.749TrpGlu: 0.749 ± 0.028	0.418TrpPhe: 0.418 ± 0.026	0.676TrpGly: 0.676 ± 0.032	0.167TrpHis: 0.167 ± 0.015	0.632TrpIle: 0.632 ± 0.03	0.712TrpLys: 0.712 ± 0.034	0.963TrpLeu: 0.963 ± 0.037	0.364TrpMet: 0.364 ± 0.023	0.459TrpAsn: 0.459 ± 0.03	0.267TrpPro: 0.267 ± 0.018	0.339TrpGln: 0.339 ± 0.023	0.42TrpArg: 0.42 ± 0.028	0.433TrpSer: 0.433 ± 0.024	0.383TrpThr: 0.383 ± 0.023	0.463TrpVal: 0.463 ± 0.026	0.111TrpTrp: 0.111 ± 0.011	0.441TrpTyr: 0.441 ± 0.033	0.0TrpXaa: 0.0 ± 0.0
Tyr	2.482TyrAla: 2.482 ± 0.057	0.571TyrCys: 0.571 ± 0.025	2.197TyrAsp: 2.197 ± 0.079	2.747TyrGlu: 2.747 ± 0.066	1.607TyrPhe: 1.607 ± 0.054	2.941TyrGly: 2.941 ± 0.065	0.687TyrHis: 0.687 ± 0.031	2.258TyrIle: 2.258 ± 0.053	1.58TyrLys: 1.58 ± 0.047	3.622TyrLeu: 3.622 ± 0.075	0.99TyrMet: 0.99 ± 0.039	1.402TyrAsn: 1.402 ± 0.044	1.356TyrPro: 1.356 ± 0.035	1.245TyrGln: 1.245 ± 0.04	2.552TyrArg: 2.552 ± 0.05	2.207TyrSer: 2.207 ± 0.058	2.019TyrThr: 2.019 ± 0.065	2.447TyrVal: 2.447 ± 0.057	0.319TyrTrp: 0.319 ± 0.02	1.695TyrTyr: 1.695 ± 0.064	0.0TyrXaa: 0.0 ± 0.0
Xaa	0.002XaaAla: 0.002 ± 0.002	0.004XaaCys: 0.004 ± 0.002	0.0XaaAsp: 0.0 ± 0.0	0.001XaaGlu: 0.001 ± 0.001	0.0XaaPhe: 0.0 ± 0.0	0.002XaaGly: 0.002 ± 0.002	0.0XaaHis: 0.0 ± 0.0	0.002XaaIle: 0.002 ± 0.002	0.0XaaLys: 0.0 ± 0.0	0.001XaaLeu: 0.001 ± 0.001	0.0XaaMet: 0.0 ± 0.0	0.0XaaAsn: 0.0 ± 0.0	0.002XaaPro: 0.002 ± 0.002	0.001XaaGln: 0.001 ± 0.001	0.001XaaArg: 0.001 ± 0.001	0.001XaaSer: 0.001 ± 0.001	0.0XaaThr: 0.0 ± 0.0	0.0XaaVal: 0.0 ± 0.0	0.0XaaTrp: 0.0 ± 0.0	0.002XaaTyr: 0.002 ± 0.002	0.029XaaXaa: 0.029 ± 0.007

Statistics based on 2596 proteins (801876 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944

Contact: Lukasz P. Kozlowski

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