Amino acid dipepetide frequency for Curvibacter sp. PAE-UM

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10-3.

For more information see sequence space article on Wikipedia.

AlaCysAspGluPheGlyHisIleLysLeuMetAsnProGlnArgSerThrValTrpTyrXaa
Ala
16.256AlaAla: 16.256 ± 0.188
1.298AlaCys: 1.298 ± 0.036
5.714AlaAsp: 5.714 ± 0.074
6.711AlaGlu: 6.711 ± 0.084
3.735AlaPhe: 3.735 ± 0.062
10.564AlaGly: 10.564 ± 0.116
2.546AlaHis: 2.546 ± 0.049
5.125AlaIle: 5.125 ± 0.072
4.041AlaLys: 4.041 ± 0.091
14.631AlaLeu: 14.631 ± 0.14
3.502AlaMet: 3.502 ± 0.059
2.621AlaAsn: 2.621 ± 0.058
5.702AlaPro: 5.702 ± 0.103
6.355AlaGln: 6.355 ± 0.097
8.703AlaArg: 8.703 ± 0.101
6.18AlaSer: 6.18 ± 0.079
5.763AlaThr: 5.763 ± 0.07
8.669AlaVal: 8.669 ± 0.096
1.956AlaTrp: 1.956 ± 0.055
2.574AlaTyr: 2.574 ± 0.053
0.0AlaXaa: 0.0 ± 0.0
Cys
1.101CysAla: 1.101 ± 0.032
0.097CysCys: 0.097 ± 0.01
0.472CysAsp: 0.472 ± 0.022
0.534CysGlu: 0.534 ± 0.021
0.265CysPhe: 0.265 ± 0.016
0.942CysGly: 0.942 ± 0.031
0.262CysHis: 0.262 ± 0.015
0.416CysIle: 0.416 ± 0.019
0.26CysLys: 0.26 ± 0.015
0.846CysLeu: 0.846 ± 0.029
0.225CysMet: 0.225 ± 0.016
0.26CysAsn: 0.26 ± 0.015
0.522CysPro: 0.522 ± 0.024
0.285CysGln: 0.285 ± 0.017
0.562CysArg: 0.562 ± 0.021
0.535CysSer: 0.535 ± 0.023
0.503CysThr: 0.503 ± 0.023
0.71CysVal: 0.71 ± 0.024
0.102CysTrp: 0.102 ± 0.01
0.199CysTyr: 0.199 ± 0.014
0.0CysXaa: 0.0 ± 0.0
Asp
6.161AspAla: 6.161 ± 0.084
0.43AspCys: 0.43 ± 0.022
2.273AspAsp: 2.273 ± 0.053
3.135AspGlu: 3.135 ± 0.067
2.026AspPhe: 2.026 ± 0.044
4.047AspGly: 4.047 ± 0.064
1.081AspHis: 1.081 ± 0.034
2.51AspIle: 2.51 ± 0.051
1.909AspLys: 1.909 ± 0.041
5.223AspLeu: 5.223 ± 0.075
1.272AspMet: 1.272 ± 0.031
1.2AspAsn: 1.2 ± 0.035
2.64AspPro: 2.64 ± 0.05
1.659AspGln: 1.659 ± 0.044
2.901AspArg: 2.901 ± 0.059
2.191AspSer: 2.191 ± 0.046
2.541AspThr: 2.541 ± 0.048
3.835AspVal: 3.835 ± 0.056
0.898AspTrp: 0.898 ± 0.028
1.42AspTyr: 1.42 ± 0.034
0.0AspXaa: 0.0 ± 0.0
Glu
6.714GluAla: 6.714 ± 0.083
0.359GluCys: 0.359 ± 0.019
2.28GluAsp: 2.28 ± 0.052
2.825GluGlu: 2.825 ± 0.058
2.005GluPhe: 2.005 ± 0.046
3.87GluGly: 3.87 ± 0.067
1.387GluHis: 1.387 ± 0.035
2.867GluIle: 2.867 ± 0.058
2.31GluLys: 2.31 ± 0.059
6.507GluLeu: 6.507 ± 0.083
1.425GluMet: 1.425 ± 0.041
1.311GluAsn: 1.311 ± 0.035
2.449GluPro: 2.449 ± 0.052
2.889GluGln: 2.889 ± 0.057
4.421GluArg: 4.421 ± 0.081
2.53GluSer: 2.53 ± 0.049
2.647GluThr: 2.647 ± 0.051
4.267GluVal: 4.267 ± 0.066
0.702GluTrp: 0.702 ± 0.029
1.067GluTyr: 1.067 ± 0.029
0.0GluXaa: 0.0 ± 0.0
Phe
3.857PheAla: 3.857 ± 0.062
0.387PheCys: 0.387 ± 0.019
2.307PheAsp: 2.307 ± 0.055
2.171PheGlu: 2.171 ± 0.048
1.41PhePhe: 1.41 ± 0.036
3.245PheGly: 3.245 ± 0.053
0.727PheHis: 0.727 ± 0.027
1.615PheIle: 1.615 ± 0.039
1.335PheLys: 1.335 ± 0.034
3.056PheLeu: 3.056 ± 0.063
0.898PheMet: 0.898 ± 0.027
1.056PheAsn: 1.056 ± 0.033
1.503PhePro: 1.503 ± 0.038
1.097PheGln: 1.097 ± 0.034
1.813PheArg: 1.813 ± 0.042
2.042PheSer: 2.042 ± 0.046
1.785PheThr: 1.785 ± 0.044
2.779PheVal: 2.779 ± 0.052
0.527PheTrp: 0.527 ± 0.022
0.887PheTyr: 0.887 ± 0.027
0.0PheXaa: 0.0 ± 0.0
Gly
8.634GlyAla: 8.634 ± 0.107
0.875GlyCys: 0.875 ± 0.032
3.569GlyAsp: 3.569 ± 0.053
4.61GlyGlu: 4.61 ± 0.069
3.114GlyPhe: 3.114 ± 0.048
6.76GlyGly: 6.76 ± 0.111
2.079GlyHis: 2.079 ± 0.043
4.062GlyIle: 4.062 ± 0.065
3.645GlyLys: 3.645 ± 0.066
9.452GlyLeu: 9.452 ± 0.112
2.378GlyMet: 2.378 ± 0.051
2.102GlyAsn: 2.102 ± 0.05
3.095GlyPro: 3.095 ± 0.059
3.787GlyGln: 3.787 ± 0.064
5.222GlyArg: 5.222 ± 0.084
4.537GlySer: 4.537 ± 0.063
4.323GlyThr: 4.323 ± 0.069
6.496GlyVal: 6.496 ± 0.079
1.4GlyTrp: 1.4 ± 0.04
2.465GlyTyr: 2.465 ± 0.047
0.0GlyXaa: 0.0 ± 0.0
His
2.725HisAla: 2.725 ± 0.052
0.285HisCys: 0.285 ± 0.017
1.134HisAsp: 1.134 ± 0.036
1.228HisGlu: 1.228 ± 0.036
0.901HisPhe: 0.901 ± 0.027
2.102HisGly: 2.102 ± 0.051
0.633HisHis: 0.633 ± 0.027
1.053HisIle: 1.053 ± 0.034
0.696HisLys: 0.696 ± 0.024
2.358HisLeu: 2.358 ± 0.048
0.508HisMet: 0.508 ± 0.022
0.542HisAsn: 0.542 ± 0.022
1.485HisPro: 1.485 ± 0.045
0.803HisGln: 0.803 ± 0.027
1.314HisArg: 1.314 ± 0.035
1.032HisSer: 1.032 ± 0.03
1.126HisThr: 1.126 ± 0.036
1.616HisVal: 1.616 ± 0.041
0.412HisTrp: 0.412 ± 0.02
0.7HisTyr: 0.7 ± 0.027
0.0HisXaa: 0.0 ± 0.0
Ile
5.755IleAla: 5.755 ± 0.089
0.433IleCys: 0.433 ± 0.022
2.835IleAsp: 2.835 ± 0.058
3.27IleGlu: 3.27 ± 0.067
1.301IlePhe: 1.301 ± 0.037
4.047IleGly: 4.047 ± 0.059
0.861IleHis: 0.861 ± 0.033
1.74IleIle: 1.74 ± 0.051
1.722IleLys: 1.722 ± 0.044
3.645IleLeu: 3.645 ± 0.064
0.919IleMet: 0.919 ± 0.025
1.514IleAsn: 1.514 ± 0.038
2.139IlePro: 2.139 ± 0.048
1.402IleGln: 1.402 ± 0.039
2.785IleArg: 2.785 ± 0.047
2.604IleSer: 2.604 ± 0.053
2.614IleThr: 2.614 ± 0.053
3.535IleVal: 3.535 ± 0.068
0.485IleTrp: 0.485 ± 0.025
1.09IleTyr: 1.09 ± 0.033
0.0IleXaa: 0.0 ± 0.0
Lys
4.416LysAla: 4.416 ± 0.083
0.197LysCys: 0.197 ± 0.014
1.896LysAsp: 1.896 ± 0.042
1.77LysGlu: 1.77 ± 0.045
1.114LysPhe: 1.114 ± 0.03
2.689LysGly: 2.689 ± 0.059
0.763LysHis: 0.763 ± 0.03
1.767LysIle: 1.767 ± 0.049
1.696LysLys: 1.696 ± 0.05
4.068LysLeu: 4.068 ± 0.065
0.971LysMet: 0.971 ± 0.034
1.127LysAsn: 1.127 ± 0.031
2.281LysPro: 2.281 ± 0.057
1.369LysGln: 1.369 ± 0.038
2.311LysArg: 2.311 ± 0.046
2.003LysSer: 2.003 ± 0.048
2.281LysThr: 2.281 ± 0.047
2.838LysVal: 2.838 ± 0.055
0.377LysTrp: 0.377 ± 0.018
0.799LysTyr: 0.799 ± 0.03
0.0LysXaa: 0.0 ± 0.0
Leu
14.993LeuAla: 14.993 ± 0.153
1.026LeuCys: 1.026 ± 0.033
5.838LeuAsp: 5.838 ± 0.072
5.229LeuGlu: 5.229 ± 0.075
3.459LeuPhe: 3.459 ± 0.063
9.177LeuGly: 9.177 ± 0.11
2.475LeuHis: 2.475 ± 0.053
4.592LeuIle: 4.592 ± 0.075
4.346LeuLys: 4.346 ± 0.066
12.634LeuLeu: 12.634 ± 0.171
2.75LeuMet: 2.75 ± 0.058
2.906LeuAsn: 2.906 ± 0.053
6.279LeuPro: 6.279 ± 0.076
5.076LeuGln: 5.076 ± 0.087
7.588LeuArg: 7.588 ± 0.095
6.127LeuSer: 6.127 ± 0.09
5.512LeuThr: 5.512 ± 0.082
8.073LeuVal: 8.073 ± 0.096
1.477LeuTrp: 1.477 ± 0.045
2.201LeuTyr: 2.201 ± 0.046
0.0LeuXaa: 0.0 ± 0.0
Met
3.191MetAla: 3.191 ± 0.055
0.177MetCys: 0.177 ± 0.012
1.231MetAsp: 1.231 ± 0.032
1.221MetGlu: 1.221 ± 0.035
0.787MetPhe: 0.787 ± 0.029
2.043MetGly: 2.043 ± 0.049
0.557MetHis: 0.557 ± 0.02
1.021MetIle: 1.021 ± 0.03
1.304MetLys: 1.304 ± 0.037
2.741MetLeu: 2.741 ± 0.057
0.613MetMet: 0.613 ± 0.027
0.937MetAsn: 0.937 ± 0.031
1.474MetPro: 1.474 ± 0.038
1.183MetGln: 1.183 ± 0.033
1.629MetArg: 1.629 ± 0.033
1.712MetSer: 1.712 ± 0.031
1.48MetThr: 1.48 ± 0.033
1.813MetVal: 1.813 ± 0.041
0.238MetTrp: 0.238 ± 0.016
0.434MetTyr: 0.434 ± 0.02
0.0MetXaa: 0.0 ± 0.0
Asn
3.054AsnAla: 3.054 ± 0.06
0.267AsnCys: 0.267 ± 0.017
1.257AsnAsp: 1.257 ± 0.036
1.337AsnGlu: 1.337 ± 0.039
1.052AsnPhe: 1.052 ± 0.031
2.103AsnGly: 2.103 ± 0.05
0.527AsnHis: 0.527 ± 0.023
1.271AsnIle: 1.271 ± 0.036
0.957AsnLys: 0.957 ± 0.034
2.705AsnLeu: 2.705 ± 0.052
0.653AsnMet: 0.653 ± 0.023
0.718AsnAsn: 0.718 ± 0.029
1.86AsnPro: 1.86 ± 0.051
0.948AsnGln: 0.948 ± 0.03
1.667AsnArg: 1.667 ± 0.04
1.203AsnSer: 1.203 ± 0.033
1.4AsnThr: 1.4 ± 0.037
1.979AsnVal: 1.979 ± 0.043
0.427AsnTrp: 0.427 ± 0.02
0.702AsnTyr: 0.702 ± 0.028
0.0AsnXaa: 0.0 ± 0.0
Pro
7.087ProAla: 7.087 ± 0.117
0.366ProCys: 0.366 ± 0.019
2.834ProAsp: 2.834 ± 0.056
3.446ProGlu: 3.446 ± 0.058
1.682ProPhe: 1.682 ± 0.04
4.69ProGly: 4.69 ± 0.063
1.14ProHis: 1.14 ± 0.037
1.873ProIle: 1.873 ± 0.046
1.622ProLys: 1.622 ± 0.04
5.387ProLeu: 5.387 ± 0.074
1.296ProMet: 1.296 ± 0.033
1.262ProAsn: 1.262 ± 0.043
2.408ProPro: 2.408 ± 0.055
2.369ProGln: 2.369 ± 0.049
2.859ProArg: 2.859 ± 0.061
2.465ProSer: 2.465 ± 0.051
2.394ProThr: 2.394 ± 0.047
4.336ProVal: 4.336 ± 0.06
0.793ProTrp: 0.793 ± 0.023
1.202ProTyr: 1.202 ± 0.031
0.0ProXaa: 0.0 ± 0.0
Gln
6.392GlnAla: 6.392 ± 0.097
0.294GlnCys: 0.294 ± 0.015
1.89GlnAsp: 1.89 ± 0.041
1.911GlnGlu: 1.911 ± 0.05
1.269GlnPhe: 1.269 ± 0.034
3.552GlnGly: 3.552 ± 0.07
1.077GlnHis: 1.077 ± 0.035
1.887GlnIle: 1.887 ± 0.04
1.317GlnLys: 1.317 ± 0.036
4.857GlnLeu: 4.857 ± 0.074
1.019GlnMet: 1.019 ± 0.034
0.91GlnAsn: 0.91 ± 0.031
2.458GlnPro: 2.458 ± 0.051
2.333GlnGln: 2.333 ± 0.055
3.536GlnArg: 3.536 ± 0.068
2.014GlnSer: 2.014 ± 0.043
2.203GlnThr: 2.203 ± 0.043
3.22GlnVal: 3.22 ± 0.052
0.635GlnTrp: 0.635 ± 0.026
0.833GlnTyr: 0.833 ± 0.034
0.0GlnXaa: 0.0 ± 0.0
Arg
7.218ArgAla: 7.218 ± 0.087
0.569ArgCys: 0.569 ± 0.023
3.287ArgAsp: 3.287 ± 0.062
4.258ArgGlu: 4.258 ± 0.065
2.529ArgPhe: 2.529 ± 0.049
4.539ArgGly: 4.539 ± 0.074
1.774ArgHis: 1.774 ± 0.048
3.371ArgIle: 3.371 ± 0.053
2.314ArgLys: 2.314 ± 0.055
7.825ArgLeu: 7.825 ± 0.119
1.898ArgMet: 1.898 ± 0.045
1.826ArgAsn: 1.826 ± 0.044
3.03ArgPro: 3.03 ± 0.056
3.171ArgGln: 3.171 ± 0.054
4.547ArgArg: 4.547 ± 0.069
3.315ArgSer: 3.315 ± 0.062
2.965ArgThr: 2.965 ± 0.052
4.886ArgVal: 4.886 ± 0.07
1.16ArgTrp: 1.16 ± 0.033
1.854ArgTyr: 1.854 ± 0.042
0.0ArgXaa: 0.0 ± 0.0
Ser
6.017SerAla: 6.017 ± 0.085
0.494SerCys: 0.494 ± 0.023
2.478SerAsp: 2.478 ± 0.054
2.674SerGlu: 2.674 ± 0.052
2.021SerPhe: 2.021 ± 0.044
4.996SerGly: 4.996 ± 0.074
1.144SerHis: 1.144 ± 0.033
2.395SerIle: 2.395 ± 0.048
1.803SerLys: 1.803 ± 0.043
5.527SerLeu: 5.527 ± 0.078
1.405SerMet: 1.405 ± 0.035
1.433SerAsn: 1.433 ± 0.042
2.77SerPro: 2.77 ± 0.052
1.905SerGln: 1.905 ± 0.043
3.328SerArg: 3.328 ± 0.054
3.054SerSer: 3.054 ± 0.058
2.788SerThr: 2.788 ± 0.054
3.862SerVal: 3.862 ± 0.067
0.79SerTrp: 0.79 ± 0.029
1.286SerTyr: 1.286 ± 0.038
0.0SerXaa: 0.0 ± 0.0
Thr
5.777ThrAla: 5.777 ± 0.075
0.388ThrCys: 0.388 ± 0.023
2.359ThrAsp: 2.359 ± 0.049
2.476ThrGlu: 2.476 ± 0.05
1.622ThrPhe: 1.622 ± 0.041
4.695ThrGly: 4.695 ± 0.069
1.16ThrHis: 1.16 ± 0.033
2.087ThrIle: 2.087 ± 0.052
1.313ThrLys: 1.313 ± 0.04
6.613ThrLeu: 6.613 ± 0.082
1.175ThrMet: 1.175 ± 0.034
1.144ThrAsn: 1.144 ± 0.038
3.495ThrPro: 3.495 ± 0.054
2.16ThrGln: 2.16 ± 0.05
3.37ThrArg: 3.37 ± 0.057
2.6ThrSer: 2.6 ± 0.06
2.723ThrThr: 2.723 ± 0.06
4.35ThrVal: 4.35 ± 0.074
0.653ThrTrp: 0.653 ± 0.026
1.056ThrTyr: 1.056 ± 0.033
0.0ThrXaa: 0.0 ± 0.0
Val
9.171ValAla: 9.171 ± 0.098
0.73ValCys: 0.73 ± 0.028
3.885ValAsp: 3.885 ± 0.058
4.16ValGlu: 4.16 ± 0.06
2.662ValPhe: 2.662 ± 0.048
5.412ValGly: 5.412 ± 0.079
1.634ValHis: 1.634 ± 0.047
3.541ValIle: 3.541 ± 0.066
2.779ValLys: 2.779 ± 0.061
8.973ValLeu: 8.973 ± 0.107
1.956ValMet: 1.956 ± 0.043
2.177ValAsn: 2.177 ± 0.045
4.0ValPro: 4.0 ± 0.063
3.155ValGln: 3.155 ± 0.06
4.893ValArg: 4.893 ± 0.069
4.013ValSer: 4.013 ± 0.066
4.11ValThr: 4.11 ± 0.064
6.337ValVal: 6.337 ± 0.092
1.053ValTrp: 1.053 ± 0.031
1.657ValTyr: 1.657 ± 0.043
0.0ValXaa: 0.0 ± 0.0
Trp
1.41TrpAla: 1.41 ± 0.04
0.162TrpCys: 0.162 ± 0.013
0.605TrpAsp: 0.605 ± 0.022
0.57TrpGlu: 0.57 ± 0.023
0.6TrpPhe: 0.6 ± 0.026
1.071TrpGly: 1.071 ± 0.037
0.357TrpHis: 0.357 ± 0.018
0.647TrpIle: 0.647 ± 0.026
0.508TrpLys: 0.508 ± 0.022
2.26TrpLeu: 2.26 ± 0.059
0.426TrpMet: 0.426 ± 0.018
0.413TrpAsn: 0.413 ± 0.023
0.731TrpPro: 0.731 ± 0.027
0.731TrpGln: 0.731 ± 0.03
1.187TrpArg: 1.187 ± 0.037
0.776TrpSer: 0.776 ± 0.029
0.702TrpThr: 0.702 ± 0.026
1.011TrpVal: 1.011 ± 0.033
0.307TrpTrp: 0.307 ± 0.018
0.319TrpTyr: 0.319 ± 0.019
0.0TrpXaa: 0.0 ± 0.0
Tyr
2.715TyrAla: 2.715 ± 0.056
0.262TyrCys: 0.262 ± 0.015
1.208TyrAsp: 1.208 ± 0.034
1.295TyrGlu: 1.295 ± 0.034
0.943TyrPhe: 0.943 ± 0.03
2.061TyrGly: 2.061 ± 0.042
0.472TyrHis: 0.472 ± 0.02
0.854TyrIle: 0.854 ± 0.026
0.858TyrLys: 0.858 ± 0.035
2.45TyrLeu: 2.45 ± 0.048
0.497TyrMet: 0.497 ± 0.02
0.626TyrAsn: 0.626 ± 0.026
1.192TyrPro: 1.192 ± 0.033
0.931TyrGln: 0.931 ± 0.031
1.699TyrArg: 1.699 ± 0.042
1.237TyrSer: 1.237 ± 0.035
1.358TyrThr: 1.358 ± 0.039
1.684TyrVal: 1.684 ± 0.043
0.403TyrTrp: 0.403 ± 0.018
0.594TyrTyr: 0.594 ± 0.024
0.0TyrXaa: 0.0 ± 0.0
Xaa
0.0XaaAla: 0.0 ± 0.0
0.0XaaCys: 0.0 ± 0.0
0.0XaaAsp: 0.0 ± 0.0
0.0XaaGlu: 0.0 ± 0.0
0.0XaaPhe: 0.0 ± 0.0
0.0XaaGly: 0.0 ± 0.0
0.0XaaHis: 0.0 ± 0.0
0.0XaaIle: 0.0 ± 0.0
0.0XaaLys: 0.0 ± 0.0
0.0XaaLeu: 0.0 ± 0.0
0.0XaaMet: 0.0 ± 0.0
0.0XaaAsn: 0.0 ± 0.0
0.0XaaPro: 0.0 ± 0.0
0.0XaaGln: 0.0 ± 0.0
0.0XaaArg: 0.0 ± 0.0
0.0XaaSer: 0.0 ± 0.0
0.0XaaThr: 0.0 ± 0.0
0.0XaaVal: 0.0 ± 0.0
0.0XaaTrp: 0.0 ± 0.0
0.0XaaTyr: 0.0 ± 0.0
0.0XaaXaa: 0.0 ± 0.0
Statistics based on 3353 proteins (1077378 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski