Rhodovulum sulfidophilum (Rhodobacter sulfidophilus)

Taxonomy: cellular organisms; Bacteria; Proteobacteria; Alphaproteobacteria; Rhodobacterales; Rhodobacteraceae; Rhodovulum

Average proteome isoelectric point is 6.52

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 4359 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A0D6AXU5|A0A0D6AXU5_RHOSU Uncharacterized protein OS=Rhodovulum sulfidophilum OX=35806 GN=NHU_00421 PE=4 SV=1
MM1 pKa = 7.23TVNNTLRR8 pKa = 11.84GATAAICIVFGAGTASAAVIDD29 pKa = 4.69LGFVLDD35 pKa = 4.32GSGSVSSYY43 pKa = 11.59DD44 pKa = 3.46FDD46 pKa = 3.99SATAALSAALSQIPTSGDD64 pKa = 2.74NEE66 pKa = 3.91YY67 pKa = 10.61RR68 pKa = 11.84VAVTSYY74 pKa = 10.13GYY76 pKa = 10.55SSYY79 pKa = 10.9TVVPPTVVTAANIASIQSTVLTAYY103 pKa = 9.81KK104 pKa = 10.38DD105 pKa = 3.59DD106 pKa = 4.56GGTDD110 pKa = 3.11TAGAITYY117 pKa = 8.42ITDD120 pKa = 4.01LFLDD124 pKa = 4.83DD125 pKa = 5.04GLGDD129 pKa = 3.51TTLINITTDD138 pKa = 3.51GSPNSQSLTEE148 pKa = 4.14NAALTANTAGVDD160 pKa = 4.5GISLEE165 pKa = 4.28AVGSGVSSPYY175 pKa = 10.63ALSNMARR182 pKa = 11.84IAGLGTSGDD191 pKa = 3.39ADD193 pKa = 3.72AGVIAADD200 pKa = 4.92LDD202 pKa = 4.17SLPNATDD209 pKa = 3.11TGFVIPVADD218 pKa = 4.56FDD220 pKa = 4.91AYY222 pKa = 10.52GAAIEE227 pKa = 4.41AKK229 pKa = 9.24IGQVIDD235 pKa = 3.73DD236 pKa = 4.03TGGDD240 pKa = 3.53TGVVPLPAAMPMLLAGLGVFGFVRR264 pKa = 11.84RR265 pKa = 11.84RR266 pKa = 11.84QTAGG270 pKa = 2.89

Molecular weight: 26.86 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A0D6B961|A0A0D6B961_RHOSU Radical SAM domain protein OS=Rhodovulum sulfidophilum OX=35806 GN=NHU_04221 PE=4 SV=1
MM1 pKa = 7.98RR2 pKa = 11.84GQLSVRR8 pKa = 11.84ISLPRR13 pKa = 11.84WRR15 pKa = 11.84LAVAIVAVRR24 pKa = 11.84VFAVVPAQIPARR36 pKa = 11.84WVRR39 pKa = 11.84GVGGWVARR47 pKa = 11.84GVRR50 pKa = 11.84VKK52 pKa = 10.6RR53 pKa = 11.84SRR55 pKa = 11.84GKK57 pKa = 9.64PRR59 pKa = 11.84LLVGGWQATGDD70 pKa = 3.78GSPPHH75 pKa = 6.59SLPKK79 pKa = 9.42GAKK82 pKa = 8.72SAVVRR87 pKa = 11.84PKK89 pKa = 10.42EE90 pKa = 3.73

Molecular weight: 9.72 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

4359

0

4359

1350422

39

4373

309.8

33.45

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

13.533 ± 0.065

0.918 ± 0.013

5.829 ± 0.037

6.052 ± 0.033

3.501 ± 0.021

9.081 ± 0.054

1.955 ± 0.019

4.738 ± 0.028

2.584 ± 0.033

10.523 ± 0.048

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.597 ± 0.021

2.117 ± 0.021

5.567 ± 0.039

2.769 ± 0.02

7.832 ± 0.057

4.926 ± 0.034

5.168 ± 0.043

6.919 ± 0.031

1.378 ± 0.015

2.012 ± 0.019
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski