Chrysanthemum virus B (CVB)

Taxonomy: Viruses; Riboviria; Orthornavirae; Kitrinoviricota; Alsuviricetes; Tymovirales; Betaflexiviridae; Quinvirinae; Carlavirus

Average proteome isoelectric point is 6.87

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 6 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|Q70HV5|Q70HV5_CVB Capsid protein OS=Chrysanthemum virus B OX=12165 GN=cp PE=3 SV=1
MM1 pKa = 7.59PPKK4 pKa = 9.63PAPGDD9 pKa = 3.68TEE11 pKa = 4.95GNASGSTPTPPPPPPAQTAEE31 pKa = 4.07EE32 pKa = 4.03ARR34 pKa = 11.84LRR36 pKa = 11.84LAEE39 pKa = 4.04MEE41 pKa = 4.14RR42 pKa = 11.84EE43 pKa = 3.96RR44 pKa = 11.84EE45 pKa = 3.96QEE47 pKa = 3.95QLLDD51 pKa = 3.89EE52 pKa = 4.78MNSNTPAEE60 pKa = 4.24DD61 pKa = 3.05ARR63 pKa = 11.84NISRR67 pKa = 11.84LTQLAALLRR76 pKa = 11.84RR77 pKa = 11.84EE78 pKa = 4.05QTNVHH83 pKa = 4.9VTNMALEE90 pKa = 4.31IGRR93 pKa = 11.84PALQPPPNMRR103 pKa = 11.84GDD105 pKa = 3.82PTNMYY110 pKa = 9.86SQVSTDD116 pKa = 4.22FLWKK120 pKa = 10.26IKK122 pKa = 8.41PQRR125 pKa = 11.84ISNNMATSEE134 pKa = 4.25DD135 pKa = 3.59MVKK138 pKa = 10.08IQVALEE144 pKa = 3.93GLGVPTEE151 pKa = 4.28SVKK154 pKa = 10.7EE155 pKa = 3.94VIIRR159 pKa = 11.84LVLNCANTSSSVYY172 pKa = 9.96QDD174 pKa = 3.22PKK176 pKa = 11.47GVIEE180 pKa = 4.22WDD182 pKa = 3.08GGAIIVDD189 pKa = 3.85DD190 pKa = 4.06VVGVITEE197 pKa = 4.13HH198 pKa = 4.79STLRR202 pKa = 11.84KK203 pKa = 9.18VCRR206 pKa = 11.84LYY208 pKa = 11.16AAVAWNYY215 pKa = 7.52MHH217 pKa = 7.29LQQTPPSDD225 pKa = 3.01WSAMGFHH232 pKa = 7.13PNVKK236 pKa = 9.69YY237 pKa = 10.54AAFDD241 pKa = 3.53FFDD244 pKa = 3.86YY245 pKa = 11.18VEE247 pKa = 4.49NGAAIRR253 pKa = 11.84PSGGIVPKK261 pKa = 8.44PTRR264 pKa = 11.84AEE266 pKa = 3.78YY267 pKa = 10.32VAYY270 pKa = 8.7NTYY273 pKa = 11.16KK274 pKa = 10.18MLALNKK280 pKa = 10.13ANNNDD285 pKa = 3.13TFGNFDD291 pKa = 3.47SAITGGRR298 pKa = 11.84QGPAIHH304 pKa = 6.74NNLNNANNKK313 pKa = 7.32TLL315 pKa = 3.5

Molecular weight: 34.63 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|Q6ZZB7|Q6ZZB7_CVB 7 kDa protein OS=Chrysanthemum virus B OX=12165 GN=TGB3 PE=3 SV=1
MM1 pKa = 7.7DD2 pKa = 5.51VIVKK6 pKa = 8.46MLILRR11 pKa = 11.84KK12 pKa = 9.21FVEE15 pKa = 4.27QGNVCPIHH23 pKa = 6.7LCVDD27 pKa = 3.66IYY29 pKa = 11.39KK30 pKa = 10.33RR31 pKa = 11.84AFPRR35 pKa = 11.84SVNKK39 pKa = 10.0GRR41 pKa = 11.84SSYY44 pKa = 10.65ARR46 pKa = 11.84RR47 pKa = 11.84RR48 pKa = 11.84RR49 pKa = 11.84ALEE52 pKa = 3.44LGRR55 pKa = 11.84CHH57 pKa = 6.5RR58 pKa = 11.84CYY60 pKa = 10.09RR61 pKa = 11.84VYY63 pKa = 10.63PPLFPEE69 pKa = 5.31ISRR72 pKa = 11.84CDD74 pKa = 3.38NRR76 pKa = 11.84TCVPGISYY84 pKa = 10.39NSKK87 pKa = 9.44VRR89 pKa = 11.84DD90 pKa = 3.63YY91 pKa = 10.73ILWGVTEE98 pKa = 4.88VIPHH102 pKa = 6.76PGYY105 pKa = 11.18NFF107 pKa = 3.42

Molecular weight: 12.58 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

6

0

6

2906

64

2082

484.3

54.57

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

6.71 ± 0.683

2.753 ± 0.542

4.233 ± 0.218

7.983 ± 1.672

4.852 ± 0.781

7.054 ± 0.313

2.959 ± 0.331

4.852 ± 0.483

6.228 ± 1.237

9.601 ± 0.674

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.134 ± 0.365

4.783 ± 0.905

4.68 ± 1.353

2.719 ± 0.467

5.712 ± 0.746

6.641 ± 0.344

4.99 ± 0.565

6.435 ± 1.039

1.032 ± 0.239

3.648 ± 0.326
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski