Pedobacter yulinensis
Average proteome isoelectric point is 7.08
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 3772 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A2T3HND6|A0A2T3HND6_9SPHI Uncharacterized protein OS=Pedobacter yulinensis OX=2126353 GN=C7T94_03920 PE=4 SV=1
MM1 pKa = 6.72 TQSDD5 pKa = 4.87 LLALLQQGYY14 pKa = 8.99 PGGAGSGSMDD24 pKa = 3.34 ISTLQNPAYY33 pKa = 8.37 TALFTQLGITSTTIPLNNSTGFAAEE58 pKa = 3.99 GANIVFKK65 pKa = 11.4 GNVNGSFFGLVNPQVTIQFSPDD87 pKa = 3.36 SVNSGAFILLMHH99 pKa = 6.84 FALPGAWTFSQSIPFFAGSIYY120 pKa = 9.82 DD121 pKa = 3.85 TLVLDD126 pKa = 4.13 TSVAPALVMTSGPATDD142 pKa = 4.3 PVNNAAATASGLYY155 pKa = 10.13 FYY157 pKa = 11.26 GLFSHH162 pKa = 6.3 TQGPASFTLALSLLEE177 pKa = 4.6 AFTSEE182 pKa = 4.12 STVFGPVGLDD192 pKa = 2.97 MSVPGQEE199 pKa = 4.53 SINMQLALGSGTISNLFGQGSVSLTLSVTLFAGLLPGIDD238 pKa = 3.58 QYY240 pKa = 12.02 QKK242 pKa = 11.2 GVLFTASLAFGQSTLNLGALLSGEE266 pKa = 4.05 ADD268 pKa = 3.07 GVLNLEE274 pKa = 4.36 VTGTALAIDD283 pKa = 4.15 EE284 pKa = 4.68 SAINTYY290 pKa = 10.33 FGSNNGIVSSLPGQFQTDD308 pKa = 3.41 EE309 pKa = 4.21 SLIAITAMNFGIGLTSHH326 pKa = 5.94 NLEE329 pKa = 4.14 YY330 pKa = 11.56 AMMTISVLQGEE341 pKa = 5.06 LWNVWMNGSTPVISIGNLAFTFSIFQPFGASPLYY375 pKa = 9.65 EE376 pKa = 4.29 FNFAGIFEE384 pKa = 5.1 LGGVVPILIQAQLGNAEE401 pKa = 4.52 DD402 pKa = 5.25 LILSGQLQPGEE413 pKa = 4.3 EE414 pKa = 4.31 PGVAALIAAFYY425 pKa = 8.4 PQQTTVPADD434 pKa = 3.52 LVFEE438 pKa = 5.24 KK439 pKa = 10.2 IDD441 pKa = 3.69 FQADD445 pKa = 3.01 ISQGTFNADD454 pKa = 3.08 LVIAGEE460 pKa = 4.26 WEE462 pKa = 4.31 FGFGDD467 pKa = 3.44 NGSIAFEE474 pKa = 4.01 EE475 pKa = 4.37 LSMFFSYY482 pKa = 10.8 DD483 pKa = 3.05 SSNTPFIQAGVEE495 pKa = 4.37 GIFRR499 pKa = 11.84 IDD501 pKa = 3.15 QTNQFDD507 pKa = 3.95 IALTLSNTNFTFSGAWKK524 pKa = 8.9 DD525 pKa = 3.21 TGTPITYY532 pKa = 9.44 IDD534 pKa = 3.25 IVMALGLYY542 pKa = 9.15 GLPQLPDD549 pKa = 3.14 IDD551 pKa = 5.41 LSLTGAKK558 pKa = 8.75 LTFDD562 pKa = 3.73 SQNLIFTFEE571 pKa = 4.57 LDD573 pKa = 3.54 SKK575 pKa = 11.57 SFGDD579 pKa = 3.82 ALIIAGKK586 pKa = 8.25 STTGAWGFVFGLEE599 pKa = 4.07 ATPKK603 pKa = 10.58 GEE605 pKa = 5.11 PINIDD610 pKa = 2.86 ITNIPVVGNLVPAGDD625 pKa = 4.22 DD626 pKa = 3.39 TLSITNLRR634 pKa = 11.84 FVGATNSLPAVTLSAAQQAIVGTQLSSGITLSIDD668 pKa = 3.6 FAVGTLLSEE677 pKa = 4.29 SFTVRR682 pKa = 11.84 FALSDD687 pKa = 3.57 GSQYY691 pKa = 9.15 DD692 pKa = 3.96 TPPPPPAQSTTLWQPPAPQQSGLPASAPAGSLATWVKK729 pKa = 8.53 VQRR732 pKa = 11.84 SFGPVQIDD740 pKa = 3.36 SVGFQIVNGNSLALLVSGGVSLGGLSIGLTALEE773 pKa = 3.91 AVIPISKK780 pKa = 9.72 PFTPSFSLGGLEE792 pKa = 4.11 VAYY795 pKa = 9.2 TGTGFSVAGGLLTVPGKK812 pKa = 8.85 TPAEE816 pKa = 3.93 YY817 pKa = 10.59 LGNLSVNAGPFGVTAFGMYY836 pKa = 8.81 STVAGQPSLFAYY848 pKa = 10.21 LAVSVPLGGPPFFFVTGLAGGFGYY872 pKa = 10.4 NAQIQLPTIEE882 pKa = 4.88 NVATYY887 pKa = 9.87 PLIQAVTQTPPQSASQVQAEE907 pKa = 4.23 LQQLVSTQNNQNWLAAGIGFSSFEE931 pKa = 4.05 MLNSFALLTVSFGNTFAVALMGEE954 pKa = 4.54 SSLSVPLSTKK964 pKa = 10.84 DD965 pKa = 3.46 EE966 pKa = 4.38 IKK968 pKa = 10.74 VEE970 pKa = 4.77 PIAEE974 pKa = 4.05 AQMEE978 pKa = 4.25 ILIEE982 pKa = 3.86 FLPSNGIFAVNAQLTPSSYY1001 pKa = 11.19 VLSKK1005 pKa = 10.47 NAKK1008 pKa = 7.79 LTGGFAFDD1016 pKa = 4.06 LWFSPSPYY1024 pKa = 10.53 AGDD1027 pKa = 3.66 FVITLGGYY1035 pKa = 8.53 NPYY1038 pKa = 10.24 YY1039 pKa = 10.53 VPQKK1043 pKa = 10.21 YY1044 pKa = 9.09 YY1045 pKa = 10.13 PAVPRR1050 pKa = 11.84 LGISWQLSSLMSITGSAYY1068 pKa = 9.79 FALTPSVIMAGGSLNAVYY1086 pKa = 10.01 HH1087 pKa = 6.27 AGNLRR1092 pKa = 11.84 AWFTAEE1098 pKa = 3.61 ADD1100 pKa = 3.45 FLIRR1104 pKa = 11.84 FKK1106 pKa = 10.58 PFSFIVSISVTVGVSMKK1123 pKa = 10.75 LNLLFTTTTITLSVGLGLQLWGLPFGGTARR1153 pKa = 11.84 MDD1155 pKa = 3.61 LSIISISINFGSSLSKK1171 pKa = 10.45 PPEE1174 pKa = 4.05 VNWDD1178 pKa = 3.04 IFTEE1182 pKa = 4.41 SFLPPPQSVPPAGQASPLAPQALDD1206 pKa = 3.34 ATGVPVTTTDD1216 pKa = 3.96 SILSLSASSGLLQSISTYY1234 pKa = 7.18 TWQVDD1239 pKa = 3.37 PEE1241 pKa = 4.6 TFVMLLTLQIPATSAVVNTTDD1262 pKa = 2.95 EE1263 pKa = 4.49 HH1264 pKa = 6.4 NNPVSTPVSGSWNTNLGVGPMGVGPGGLTAALTIQITAPAAGDD1307 pKa = 3.37 EE1308 pKa = 4.56 DD1309 pKa = 4.27 VWVAQAVTGQVASGLWANTTNTMSTDD1335 pKa = 3.02 GTLQNVLVGVSLVPVPPQPATTAAIDD1361 pKa = 4.22 LSLLLYY1367 pKa = 10.82 DD1368 pKa = 4.72 PGNPVSWNWSSPQVVNTDD1386 pKa = 3.37 PYY1388 pKa = 11.71 GNDD1391 pKa = 2.84 NPMAEE1396 pKa = 4.44 MQSSLIDD1403 pKa = 3.29 ATVAATRR1410 pKa = 11.84 SSWINSLIAQGFALSNEE1427 pKa = 3.9 VDD1429 pKa = 3.48 VSDD1432 pKa = 5.35 FSSNANNLLLSAPALRR1448 pKa = 11.84 LAGEE1452 pKa = 4.35 EE1453 pKa = 4.15 KK1454 pKa = 10.91 AEE1456 pKa = 4.21 SEE1458 pKa = 4.36
Molecular weight: 152.3 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.702
IPC2_protein 3.795
IPC_protein 3.808
Toseland 3.605
ProMoST 3.948
Dawson 3.795
Bjellqvist 3.948
Wikipedia 3.694
Rodwell 3.63
Grimsley 3.503
Solomon 3.783
Lehninger 3.745
Nozaki 3.897
DTASelect 4.113
Thurlkill 3.63
EMBOSS 3.706
Sillero 3.923
Patrickios 1.481
IPC_peptide 3.783
IPC2_peptide 3.91
IPC2.peptide.svr19 3.839
Protein with the highest isoelectric point:
>tr|A0A2T3HK36|A0A2T3HK36_9SPHI Uncharacterized protein OS=Pedobacter yulinensis OX=2126353 GN=C7T94_09135 PE=4 SV=1
MM1 pKa = 7.07 KK2 pKa = 9.48 TVCRR6 pKa = 11.84 TRR8 pKa = 11.84 CRR10 pKa = 11.84 QRR12 pKa = 11.84 KK13 pKa = 7.44 SLRR16 pKa = 11.84 NDD18 pKa = 2.56 SSTRR22 pKa = 11.84 GIGLRR27 pKa = 11.84 SIAGRR32 pKa = 11.84 VALLDD37 pKa = 3.67 GSLTITCPEE46 pKa = 3.95 KK47 pKa = 10.52 GGTRR51 pKa = 11.84 ILIRR55 pKa = 11.84 FPLATVGQTGKK66 pKa = 10.37 YY67 pKa = 8.79 VPNASRR73 pKa = 11.84 PAQLTDD79 pKa = 3.45 LEE81 pKa = 4.61 TDD83 pKa = 3.57 GGQPAFYY90 pKa = 10.76 GG91 pKa = 3.65
Molecular weight: 9.82 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.351
IPC2_protein 9.619
IPC_protein 10.526
Toseland 10.716
ProMoST 10.467
Dawson 10.804
Bjellqvist 10.555
Wikipedia 11.038
Rodwell 10.935
Grimsley 10.847
Solomon 10.965
Lehninger 10.935
Nozaki 10.73
DTASelect 10.54
Thurlkill 10.716
EMBOSS 11.125
Sillero 10.745
Patrickios 10.716
IPC_peptide 10.965
IPC2_peptide 9.867
IPC2.peptide.svr19 8.54
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
3772
0
3772
1296689
25
2386
343.8
38.33
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
8.866 ± 0.041
0.794 ± 0.012
5.167 ± 0.026
5.606 ± 0.043
4.873 ± 0.027
7.403 ± 0.035
1.899 ± 0.023
6.061 ± 0.035
5.747 ± 0.035
9.91 ± 0.044
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.275 ± 0.017
4.826 ± 0.043
4.159 ± 0.024
3.92 ± 0.024
5.326 ± 0.031
6.161 ± 0.03
5.507 ± 0.038
6.484 ± 0.031
1.214 ± 0.018
3.801 ± 0.024
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here