Palleronia abyssalis
Average proteome isoelectric point is 6.14
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 4308 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A2R8BZ87|A0A2R8BZ87_9RHOB 3-oxoacyl-[acyl-carrier-protein] reductase FabG OS=Palleronia abyssalis OX=1501240 GN=fabG_7 PE=4 SV=1
MM1 pKa = 7.65 RR2 pKa = 11.84 RR3 pKa = 11.84 NTILFLAVMTGTSPAVAQEE22 pKa = 4.11 NVFGEE27 pKa = 4.31 IGASYY32 pKa = 10.35 GYY34 pKa = 11.25 GDD36 pKa = 5.28 IEE38 pKa = 5.02 DD39 pKa = 3.49 VDD41 pKa = 4.93 ANVDD45 pKa = 3.38 TFQAYY50 pKa = 9.23 GAGAFVSAAGLGIQLGGAYY69 pKa = 8.31 DD70 pKa = 3.92 TVSVDD75 pKa = 3.29 NLGDD79 pKa = 3.62 IEE81 pKa = 5.43 SYY83 pKa = 10.55 TIDD86 pKa = 2.89 GHH88 pKa = 8.05 IYY90 pKa = 10.88 GDD92 pKa = 3.76 FGSAKK97 pKa = 9.89 AGVFAGLTRR106 pKa = 11.84 VGEE109 pKa = 4.04 LEE111 pKa = 3.88 IFGVGGFDD119 pKa = 3.27 IDD121 pKa = 4.56 EE122 pKa = 4.84 DD123 pKa = 4.04 VLSYY127 pKa = 10.88 GVEE130 pKa = 3.99 GQSRR134 pKa = 11.84 VGSFTYY140 pKa = 9.88 HH141 pKa = 6.65 GYY143 pKa = 11.16 AGVADD148 pKa = 3.85 VQGPEE153 pKa = 3.97 FLEE156 pKa = 4.22 TTLYY160 pKa = 11.11 GIGADD165 pKa = 3.79 FDD167 pKa = 4.29 VSPALQLTANFDD179 pKa = 3.94 GAEE182 pKa = 3.96 LSADD186 pKa = 3.96 GIEE189 pKa = 4.29 EE190 pKa = 4.16 EE191 pKa = 5.08 LNLSTVSLGVDD202 pKa = 3.29 YY203 pKa = 11.12 YY204 pKa = 11.92 VNSQKK209 pKa = 11.09 APVRR213 pKa = 11.84 LSATVGRR220 pKa = 11.84 DD221 pKa = 3.0 MAEE224 pKa = 3.59 FDD226 pKa = 4.67 GFDD229 pKa = 4.26 ADD231 pKa = 4.01 GLSIGVGAAVLFGNQPNSNRR251 pKa = 11.84 EE252 pKa = 3.81 KK253 pKa = 11.01 LFDD256 pKa = 3.67 STGLPFF262 pKa = 5.1
Molecular weight: 27.33 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.697
IPC2_protein 3.706
IPC_protein 3.719
Toseland 3.49
ProMoST 3.872
Dawson 3.706
Bjellqvist 3.859
Wikipedia 3.643
Rodwell 3.541
Grimsley 3.401
Solomon 3.694
Lehninger 3.656
Nozaki 3.821
DTASelect 4.05
Thurlkill 3.554
EMBOSS 3.656
Sillero 3.834
Patrickios 1.1
IPC_peptide 3.694
IPC2_peptide 3.808
IPC2.peptide.svr19 3.748
Protein with the highest isoelectric point:
>tr|A0A2R8BXY6|A0A2R8BXY6_9RHOB Pyrophosphatase PpaX OS=Palleronia abyssalis OX=1501240 GN=ppaX PE=4 SV=1
MM1 pKa = 7.45 KK2 pKa = 9.61 RR3 pKa = 11.84 TFQPSNLVRR12 pKa = 11.84 KK13 pKa = 9.18 RR14 pKa = 11.84 RR15 pKa = 11.84 HH16 pKa = 4.42 GFRR19 pKa = 11.84 ARR21 pKa = 11.84 MATKK25 pKa = 10.37 AGRR28 pKa = 11.84 KK29 pKa = 8.54 ILNARR34 pKa = 11.84 RR35 pKa = 11.84 AQGRR39 pKa = 11.84 KK40 pKa = 9.3 KK41 pKa = 10.65 LSAA44 pKa = 3.91
Molecular weight: 5.15 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.517
IPC2_protein 11.213
IPC_protein 12.793
Toseland 12.969
ProMoST 13.451
Dawson 12.969
Bjellqvist 12.954
Wikipedia 13.437
Rodwell 12.676
Grimsley 12.998
Solomon 13.451
Lehninger 13.364
Nozaki 12.969
DTASelect 12.954
Thurlkill 12.969
EMBOSS 13.466
Sillero 12.969
Patrickios 12.398
IPC_peptide 13.466
IPC2_peptide 12.442
IPC2.peptide.svr19 9.148
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
4308
0
4308
1309259
31
1760
303.9
32.98
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
12.306 ± 0.05
0.873 ± 0.012
6.427 ± 0.037
5.936 ± 0.04
3.618 ± 0.023
8.808 ± 0.037
2.014 ± 0.019
5.118 ± 0.029
2.696 ± 0.031
9.965 ± 0.049
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.711 ± 0.016
2.343 ± 0.019
5.251 ± 0.029
3.055 ± 0.021
7.276 ± 0.037
5.142 ± 0.027
5.587 ± 0.022
7.339 ± 0.028
1.416 ± 0.018
2.118 ± 0.018
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here