Kadiweu virus
Average proteome isoelectric point is 7.83
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 4 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A1B1X3Z6|A0A1B1X3Z6_9NIDO Isoform of A0A1B1X401 Non-structural protein 6 OS=Kadiweu virus OX=1795438 GN=orf1 PE=4 SV=1
MM1 pKa = 7.75 SYY3 pKa = 11.39 YY4 pKa = 10.76 DD5 pKa = 4.53 IAFKK9 pKa = 11.04 DD10 pKa = 3.41 NLVIQRR16 pKa = 11.84 DD17 pKa = 3.54 QKK19 pKa = 10.79 LASEE23 pKa = 4.46 ATYY26 pKa = 11.17 IMAYY30 pKa = 8.06 TIWFPAFIMTMALSVKK46 pKa = 9.98 IYY48 pKa = 10.76 QNVPNPLSGPLADD61 pKa = 4.54 NLLKK65 pKa = 10.84 RR66 pKa = 11.84 LVQKK70 pKa = 10.22 IHH72 pKa = 6.7 FFFTRR77 pKa = 11.84 KK78 pKa = 9.4 NYY80 pKa = 10.15 YY81 pKa = 7.65 YY82 pKa = 9.97 QEE84 pKa = 4.14 PVFTRR89 pKa = 11.84 DD90 pKa = 2.87 EE91 pKa = 4.08 SRR93 pKa = 11.84 ITVFMTPDD101 pKa = 4.04 FVRR104 pKa = 11.84 IDD106 pKa = 3.42 HH107 pKa = 6.74 TNLIGYY113 pKa = 7.4 CNKK116 pKa = 10.11 CNLFGHH122 pKa = 5.59 TTSTSRR128 pKa = 11.84 HH129 pKa = 4.9 TNVIDD134 pKa = 3.82 ALILAKK140 pKa = 9.88 ICKK143 pKa = 8.95 IRR145 pKa = 11.84 SYY147 pKa = 11.47 NNQTRR152 pKa = 11.84 CIKK155 pKa = 10.47 AYY157 pKa = 8.58 TVHH160 pKa = 6.85 NIKK163 pKa = 10.84 AFDD166 pKa = 4.01 KK167 pKa = 10.64 NPKK170 pKa = 10.28 AFFDD174 pKa = 4.21 LFGTPITKK182 pKa = 9.84 IPSKK186 pKa = 10.52 YY187 pKa = 10.54 ALAPKK192 pKa = 8.58 HH193 pKa = 5.39 TLVNLTTIEE202 pKa = 4.36 TNLGTIYY209 pKa = 10.52 INNTIEE215 pKa = 4.13 NPHH218 pKa = 6.81 LGYY221 pKa = 9.87 IAYY224 pKa = 8.91 EE225 pKa = 4.01 NQEE228 pKa = 3.99 QLDD231 pKa = 3.93 TSIATLSMVMEE242 pKa = 4.32 NLMVYY247 pKa = 7.4 TTLAVPDD254 pKa = 3.97 VTSMNLLKK262 pKa = 10.88 CEE264 pKa = 4.11 EE265 pKa = 4.37 EE266 pKa = 4.09 PVFTNNIDD274 pKa = 3.4 LTLAIQAEE282 pKa = 4.64 HH283 pKa = 7.21 KK284 pKa = 10.57 DD285 pKa = 3.43 NHH287 pKa = 5.88 TNWLLKK293 pKa = 10.46 AKK295 pKa = 10.44 KK296 pKa = 9.64 KK297 pKa = 10.42 DD298 pKa = 3.6 NKK300 pKa = 10.34 EE301 pKa = 4.19 SAEE304 pKa = 4.26 SAAEE308 pKa = 3.75 TSPRR312 pKa = 11.84 TKK314 pKa = 10.47 NGHH317 pKa = 4.67 VKK319 pKa = 10.38 NGKK322 pKa = 7.48 EE323 pKa = 4.25 TQQKK327 pKa = 8.06 PTEE330 pKa = 4.16 KK331 pKa = 10.57 SNPQLKK337 pKa = 9.34 PQTTLLQNTLAKK349 pKa = 10.58 SNNRR353 pKa = 11.84 QRR355 pKa = 11.84 KK356 pKa = 7.24 GHH358 pKa = 6.14 YY359 pKa = 8.68 LTFGPTFMTMLCVIGLIQPGLTICTQYY386 pKa = 11.17 LPQRR390 pKa = 11.84 EE391 pKa = 4.14 NDD393 pKa = 4.77 FIPCKK398 pKa = 10.2 YY399 pKa = 10.35 VSNLTSEE406 pKa = 4.57 YY407 pKa = 9.65 VQYY410 pKa = 10.65 HH411 pKa = 6.22 YY412 pKa = 11.64 YY413 pKa = 10.56 HH414 pKa = 6.24 VTQQACFTVNSDD426 pKa = 2.91 QYY428 pKa = 10.83 TDD430 pKa = 3.6 LVRR433 pKa = 11.84 LNMEE437 pKa = 4.63 HH438 pKa = 6.8 PHH440 pKa = 6.05 IALPVIEE447 pKa = 5.47 PKK449 pKa = 10.66 PLDD452 pKa = 3.71 NYY454 pKa = 10.13 ILEE457 pKa = 4.49 AFRR460 pKa = 11.84 GVLPYY465 pKa = 9.98 DD466 pKa = 3.64 TIIYY470 pKa = 10.31 SKK472 pKa = 10.8 FSTLQDD478 pKa = 3.66 LQILMDD484 pKa = 4.14 IYY486 pKa = 11.01 DD487 pKa = 3.69 YY488 pKa = 11.06 DD489 pKa = 3.93 YY490 pKa = 11.59 EE491 pKa = 4.42 KK492 pKa = 11.35 VLFLEE497 pKa = 4.83 EE498 pKa = 4.28 DD499 pKa = 3.61 AKK501 pKa = 10.95 SLPMDD506 pKa = 3.53 YY507 pKa = 10.12 KK508 pKa = 11.13 GKK510 pKa = 10.07 IVQTLLEE517 pKa = 4.04 QDD519 pKa = 3.84 SFVCEE524 pKa = 4.06 LPKK527 pKa = 10.78 CILFTGLNDD536 pKa = 3.32 TTTDD540 pKa = 3.01 INIKK544 pKa = 8.07 ITEE547 pKa = 4.13 RR548 pKa = 11.84 LSRR551 pKa = 11.84 RR552 pKa = 11.84 IPHH555 pKa = 6.64 APSGLPVKK563 pKa = 10.44 VDD565 pKa = 3.58 PACTHH570 pKa = 5.91 KK571 pKa = 10.53 CYY573 pKa = 10.74 CKK575 pKa = 9.96 IEE577 pKa = 4.14 EE578 pKa = 4.34 EE579 pKa = 4.46 SVKK582 pKa = 10.2 PQEE585 pKa = 4.15 IVKK588 pKa = 8.53 PTLVEE593 pKa = 4.28 PPTEE597 pKa = 4.41 FYY599 pKa = 11.3 LNTRR603 pKa = 11.84 YY604 pKa = 8.79 FQQYY608 pKa = 7.21 EE609 pKa = 4.33 LRR611 pKa = 11.84 LLDD614 pKa = 4.72 DD615 pKa = 4.4 FSNNLFQYY623 pKa = 11.13 NNYY626 pKa = 8.18 TLTRR630 pKa = 11.84 TVYY633 pKa = 10.65 KK634 pKa = 10.76 ADD636 pKa = 3.1 TCYY639 pKa = 10.96 VKK641 pKa = 10.77 EE642 pKa = 4.19 PATDD646 pKa = 3.8 FCLYY650 pKa = 10.79 DD651 pKa = 3.5 PALFATRR658 pKa = 11.84 RR659 pKa = 11.84 VVNKK663 pKa = 10.05 FGNHH667 pKa = 5.6 LEE669 pKa = 4.07 CGINHH674 pKa = 7.1 KK675 pKa = 10.41 FCEE678 pKa = 4.1 DD679 pKa = 3.39 MQLEE683 pKa = 4.68 FAPQHH688 pKa = 5.59 LTTPTVQDD696 pKa = 4.14 CDD698 pKa = 3.58 PLYY701 pKa = 10.92 TFLKK705 pKa = 8.53 EE706 pKa = 4.03 YY707 pKa = 10.93 KK708 pKa = 9.19 MDD710 pKa = 3.73 GMPTNMIKK718 pKa = 10.43 KK719 pKa = 9.7 AIKK722 pKa = 10.25 DD723 pKa = 3.42 MHH725 pKa = 6.01 NTPTGITLVKK735 pKa = 10.28 HH736 pKa = 5.73 NHH738 pKa = 4.05 TTMDD742 pKa = 3.27 VMKK745 pKa = 10.1 LAEE748 pKa = 4.06 FMQYY752 pKa = 10.58 CINKK756 pKa = 8.34 NNNYY760 pKa = 8.4 TVTARR765 pKa = 11.84 SFFFDD770 pKa = 3.8 YY771 pKa = 11.03 PDD773 pKa = 3.28 VSSLNKK779 pKa = 9.75 FLHH782 pKa = 6.11 NFTATTNVVLINGANGIPCEE802 pKa = 4.07 KK803 pKa = 10.72 AEE805 pKa = 4.52 AYY807 pKa = 10.75 NPFTDD812 pKa = 4.17 YY813 pKa = 11.16 YY814 pKa = 11.15 GKK816 pKa = 8.65 TKK818 pKa = 9.45 STNTIFLFVHH828 pKa = 6.62 YY829 pKa = 8.95 ITTPIEE835 pKa = 4.23 RR836 pKa = 11.84 TSSHH840 pKa = 7.13 HH841 pKa = 5.73 IYY843 pKa = 9.88 EE844 pKa = 4.55 WYY846 pKa = 10.29 ARR848 pKa = 11.84 CPKK851 pKa = 9.3 LTQTKK856 pKa = 10.0 ARR858 pKa = 11.84 NLARR862 pKa = 11.84 NMVFNLLDD870 pKa = 3.98 LPTDD874 pKa = 3.83 YY875 pKa = 11.23 SFKK878 pKa = 10.25 TPPIRR883 pKa = 11.84 KK884 pKa = 8.37 IVSEE888 pKa = 4.07 NVYY891 pKa = 10.04 VHH893 pKa = 6.14 YY894 pKa = 11.18 KK895 pKa = 10.94 DD896 pKa = 3.55 MFYY899 pKa = 10.18 NTVKK903 pKa = 10.5 HH904 pKa = 4.26 YY905 pKa = 9.86 TSYY908 pKa = 9.27 FTAKK912 pKa = 10.06 FNQKK916 pKa = 9.98 HH917 pKa = 4.92 EE918 pKa = 4.32 TVKK921 pKa = 10.9 DD922 pKa = 3.84 YY923 pKa = 10.9 ILEE926 pKa = 4.4 TGTIITDD933 pKa = 4.04 LTLDD937 pKa = 3.94 TYY939 pKa = 11.55 NALLNMILYY948 pKa = 10.08 RR949 pKa = 11.84 YY950 pKa = 10.15 RR951 pKa = 11.84 NFNNFIVDD959 pKa = 3.33 MTQNYY964 pKa = 9.31 NNQHH968 pKa = 5.97 PQRR971 pKa = 11.84 QDD973 pKa = 3.0 FYY975 pKa = 9.43 HH976 pKa = 5.5 QSKK979 pKa = 10.23 RR980 pKa = 11.84 AFDD983 pKa = 3.41 EE984 pKa = 4.84 GKK986 pKa = 9.1 ICEE989 pKa = 4.3 FLYY992 pKa = 10.94 HH993 pKa = 6.98 SDD995 pKa = 3.38 TMVLYY1000 pKa = 10.43 LDD1002 pKa = 4.32 CVKK1005 pKa = 11.07 DD1006 pKa = 3.97 SLDD1009 pKa = 3.57 KK1010 pKa = 10.76 IYY1012 pKa = 10.91 VYY1014 pKa = 10.64 NLRR1017 pKa = 11.84 YY1018 pKa = 10.02 GLDD1021 pKa = 3.33 VNEE1024 pKa = 3.87 FHH1026 pKa = 7.13 MYY1028 pKa = 8.14 EE1029 pKa = 3.99 LKK1031 pKa = 10.4 MPFTRR1036 pKa = 11.84 YY1037 pKa = 8.32 TMGEE1041 pKa = 4.04 LSTAIGFQYY1050 pKa = 10.48 KK1051 pKa = 10.25 GEE1053 pKa = 4.03 NYY1055 pKa = 9.68 HH1056 pKa = 5.87 YY1057 pKa = 10.28 FRR1059 pKa = 11.84 HH1060 pKa = 6.84 LYY1062 pKa = 8.51 TNPGEE1067 pKa = 4.17 YY1068 pKa = 10.79 ALTIRR1073 pKa = 11.84 KK1074 pKa = 9.34 KK1075 pKa = 10.1 EE1076 pKa = 3.91 LEE1078 pKa = 4.08 FCKK1081 pKa = 10.54 SEE1083 pKa = 4.56 IIPTPAFTPEE1093 pKa = 3.97 QPLTCYY1099 pKa = 10.22 PYY1101 pKa = 8.95 ITGFQALDD1109 pKa = 3.15 HH1110 pKa = 6.87 TINDD1114 pKa = 3.97 FGIHH1118 pKa = 5.8 LLFYY1122 pKa = 10.71 IMASSMVVAIAVNIRR1137 pKa = 11.84 DD1138 pKa = 3.5 NSYY1141 pKa = 11.45 VMVGKK1146 pKa = 10.33 LFYY1149 pKa = 11.2 VFLNTVAPWMITPNFPGSRR1168 pKa = 11.84 FISWIYY1174 pKa = 10.96 GKK1176 pKa = 10.27 ISVTNTSCEE1185 pKa = 4.0 LVLTVCILIVTFVDD1199 pKa = 3.52 VFAYY1203 pKa = 9.98 CIQFGKK1209 pKa = 10.63 FMRR1212 pKa = 11.84 NDD1214 pKa = 3.31 FNRR1217 pKa = 11.84 YY1218 pKa = 7.84 VLVHH1222 pKa = 4.82 VTLIFEE1228 pKa = 4.46 CVYY1231 pKa = 9.5 VSYY1234 pKa = 11.06 YY1235 pKa = 10.36 ILIPHH1240 pKa = 7.53 LFTGYY1245 pKa = 11.48 GMITAIIVTYY1255 pKa = 10.43 LSYY1258 pKa = 11.58 YY1259 pKa = 9.06 NYY1261 pKa = 10.1 KK1262 pKa = 10.08 RR1263 pKa = 11.84 QLPNYY1268 pKa = 8.1 LQSTVPLAVAHH1279 pKa = 5.73 QDD1281 pKa = 2.59 WVNYY1285 pKa = 10.46 RR1286 pKa = 11.84 NTTRR1290 pKa = 11.84 EE1291 pKa = 4.07 KK1292 pKa = 9.8 TDD1294 pKa = 3.25 EE1295 pKa = 3.98 AAKK1298 pKa = 10.2 AQIRR1302 pKa = 11.84 RR1303 pKa = 11.84 LMNVNITEE1311 pKa = 3.9 VDD1313 pKa = 3.72 SNKK1316 pKa = 9.75 LRR1318 pKa = 11.84 EE1319 pKa = 4.41 CVYY1322 pKa = 10.73 LAACYY1327 pKa = 9.33 QHH1329 pKa = 6.41 TVSNPTFNPRR1339 pKa = 11.84 HH1340 pKa = 5.96 ALHH1343 pKa = 6.67 IPSVSTSIMFARR1355 pKa = 11.84 DD1356 pKa = 3.58 SEE1358 pKa = 4.59 VMSTIQNQSNTTIKK1372 pKa = 10.39 NHH1374 pKa = 5.99 SAASNPSVPHH1384 pKa = 6.55 LALNLPVTINPLITFSTGKK1403 pKa = 10.07 YY1404 pKa = 9.66 DD1405 pKa = 3.14 SSLRR1409 pKa = 11.84 GAIVNGYY1416 pKa = 10.04 IYY1418 pKa = 10.12 IQRR1421 pKa = 11.84 HH1422 pKa = 4.94 LFGEE1426 pKa = 4.39 TKK1428 pKa = 10.25 KK1429 pKa = 10.56 QFEE1432 pKa = 4.41 LCYY1435 pKa = 10.94 NNGAGLDD1442 pKa = 3.33 KK1443 pKa = 10.89 CKK1445 pKa = 10.54 NLEE1448 pKa = 3.88 RR1449 pKa = 11.84 SKK1451 pKa = 11.59 YY1452 pKa = 10.41 DD1453 pKa = 3.01 IDD1455 pKa = 3.58 SAEE1458 pKa = 4.47 IIGTLIRR1465 pKa = 11.84 IPLINKK1471 pKa = 9.66 DD1472 pKa = 4.15 SVPKK1476 pKa = 10.35 IVVHH1480 pKa = 6.2 PNPLTYY1486 pKa = 10.12 TGPITLYY1493 pKa = 10.05 LSKK1496 pKa = 11.09 YY1497 pKa = 10.04 DD1498 pKa = 3.93 VEE1500 pKa = 4.24 LQKK1503 pKa = 10.77 PVLCMHH1509 pKa = 6.9 TGFVEE1514 pKa = 4.49 EE1515 pKa = 4.29 GHH1517 pKa = 6.79 HH1518 pKa = 7.23 DD1519 pKa = 3.32 IKK1521 pKa = 10.4 TVFGDD1526 pKa = 3.49 CGGMLFTQEE1535 pKa = 3.8 GQLLGLHH1542 pKa = 6.59 CAGSDD1547 pKa = 3.26 DD1548 pKa = 5.73 VSFQDD1553 pKa = 4.22 IATGKK1558 pKa = 8.28 PNIWTSYY1565 pKa = 7.98 KK1566 pKa = 8.58 TQHH1569 pKa = 6.73 PSEE1572 pKa = 4.08 IMITLDD1578 pKa = 4.76 SKK1580 pKa = 11.52 INLQNPEE1587 pKa = 4.19 NYY1589 pKa = 9.65 DD1590 pKa = 3.34 FSSRR1594 pKa = 11.84 TVIYY1598 pKa = 7.01 THH1600 pKa = 6.48 PLRR1603 pKa = 11.84 SIRR1606 pKa = 11.84 ATLEE1610 pKa = 3.67 TLQFLTNPTNTPIAYY1625 pKa = 9.57 DD1626 pKa = 3.36 NRR1628 pKa = 11.84 LWSDD1632 pKa = 3.28 FRR1634 pKa = 11.84 ISAEE1638 pKa = 3.89 DD1639 pKa = 3.66 YY1640 pKa = 10.36 KK1641 pKa = 11.11 RR1642 pKa = 11.84 CGYY1645 pKa = 10.04 NVDD1648 pKa = 3.22 YY1649 pKa = 9.1 KK1650 pKa = 11.13 TFVNNFKK1657 pKa = 10.41 TYY1659 pKa = 7.11 TTTLINGTRR1668 pKa = 11.84 SFEE1671 pKa = 4.1 SCIKK1675 pKa = 10.91 YY1676 pKa = 10.66 GITDD1680 pKa = 3.28 KK1681 pKa = 10.74 NTTYY1685 pKa = 10.81 RR1686 pKa = 11.84 NKK1688 pKa = 10.75 SKK1690 pKa = 10.49 ISYY1693 pKa = 9.86 NYY1695 pKa = 9.06 VPNPFEE1701 pKa = 4.37 SKK1703 pKa = 9.18 STILDD1708 pKa = 3.34 NTLDD1712 pKa = 3.57 LVYY1715 pKa = 10.96 VFIYY1719 pKa = 9.63 MFTQTHH1725 pKa = 5.6 PMFYY1729 pKa = 10.04 VAAFMIILVMILKK1742 pKa = 10.26 INLKK1746 pKa = 10.29 YY1747 pKa = 9.29 KK1748 pKa = 9.23 TALISFLCIMPHH1760 pKa = 7.8 LYY1762 pKa = 9.25 IDD1764 pKa = 3.86 HH1765 pKa = 6.5 YY1766 pKa = 11.06 FGLIYY1771 pKa = 10.62 LPLKK1775 pKa = 9.43 IRR1777 pKa = 11.84 KK1778 pKa = 9.5 SIMEE1782 pKa = 4.1 YY1783 pKa = 11.01 LKK1785 pKa = 9.39 ITTTAVATIYY1795 pKa = 9.91 TSKK1798 pKa = 11.26 ALALRR1803 pKa = 11.84 NQNLKK1808 pKa = 9.8 IAKK1811 pKa = 9.69 KK1812 pKa = 10.13 LATEE1816 pKa = 4.95 LGTKK1820 pKa = 10.17 KK1821 pKa = 10.41 NLCIHH1826 pKa = 6.97 LNTLLKK1832 pKa = 10.49 CIKK1835 pKa = 9.93 PYY1837 pKa = 10.23 PAFNNLTQVINEE1849 pKa = 4.55 IDD1851 pKa = 3.69 DD1852 pKa = 5.84 LMAKK1856 pKa = 9.51 WDD1858 pKa = 3.73 DD1859 pKa = 3.69 TYY1861 pKa = 11.74 DD1862 pKa = 3.36 AEE1864 pKa = 5.22 KK1865 pKa = 10.72 RR1866 pKa = 11.84 LNQYY1870 pKa = 9.76 IDD1872 pKa = 4.59 EE1873 pKa = 4.62 IYY1875 pKa = 10.83 KK1876 pKa = 10.39 LYY1878 pKa = 10.23 PVLFVVFEE1886 pKa = 4.13 RR1887 pKa = 11.84 TEE1889 pKa = 4.15 SYY1891 pKa = 11.14 DD1892 pKa = 3.62 EE1893 pKa = 3.99 QIKK1896 pKa = 10.65 IILSYY1901 pKa = 10.88 INEE1904 pKa = 4.26 TGHH1907 pKa = 6.72 FDD1909 pKa = 4.62 LNGFEE1914 pKa = 4.27 INYY1917 pKa = 9.71 DD1918 pKa = 3.8 EE1919 pKa = 5.62 KK1920 pKa = 11.42 EE1921 pKa = 4.04 HH1922 pKa = 5.29 TTQIIEE1928 pKa = 4.37 QNEE1931 pKa = 4.12 EE1932 pKa = 3.91 LQKK1935 pKa = 11.07 LQLRR1939 pKa = 11.84 LNTQKK1944 pKa = 10.69 VSLNKK1949 pKa = 9.98 LKK1951 pKa = 11.08 NMNSKK1956 pKa = 10.33 FDD1958 pKa = 3.86 PSTINNANIAEE1969 pKa = 4.31 LVRR1972 pKa = 11.84 YY1973 pKa = 9.63 LMIGSTPDD1981 pKa = 3.09 TLDD1984 pKa = 4.06 RR1985 pKa = 11.84 EE1986 pKa = 4.74 LLTMATEE1993 pKa = 4.51 KK1994 pKa = 10.67 LVQHH1998 pKa = 6.69 IKK2000 pKa = 10.62 AIRR2003 pKa = 11.84 IDD2005 pKa = 4.07 TEE2007 pKa = 3.95 CDD2009 pKa = 2.93 TDD2011 pKa = 4.91 LVALLSEE2018 pKa = 4.59 IYY2020 pKa = 9.72 KK2021 pKa = 10.65 HH2022 pKa = 6.63 KK2023 pKa = 11.12 DD2024 pKa = 2.86 HH2025 pKa = 6.77 ITQAFLTSNAKK2036 pKa = 9.73 DD2037 pKa = 3.31 QNFVMNNLVRR2047 pKa = 11.84 VIAQFNRR2054 pKa = 11.84 QINAQAQQKK2063 pKa = 8.67 YY2064 pKa = 8.16 VEE2066 pKa = 3.97 ARR2068 pKa = 11.84 RR2069 pKa = 11.84 IEE2071 pKa = 4.08 LEE2073 pKa = 3.83 RR2074 pKa = 11.84 KK2075 pKa = 9.09 KK2076 pKa = 10.33 EE2077 pKa = 3.83 SRR2079 pKa = 11.84 VIMEE2083 pKa = 3.71 QNNRR2087 pKa = 11.84 IRR2089 pKa = 11.84 KK2090 pKa = 6.07 MQRR2093 pKa = 11.84 QNQSIASAILHH2104 pKa = 5.61 MVHH2107 pKa = 6.92 ACFANRR2113 pKa = 11.84 FMLDD2117 pKa = 2.85 TDD2119 pKa = 5.02 LKK2121 pKa = 10.96 NEE2123 pKa = 3.93 SLKK2126 pKa = 10.57 IIKK2129 pKa = 10.22 ALIDD2133 pKa = 3.59 TNLEE2137 pKa = 4.0 LDD2139 pKa = 3.62 PTEE2142 pKa = 5.66 AEE2144 pKa = 4.04 HH2145 pKa = 6.85 QYY2147 pKa = 11.45 LSTRR2151 pKa = 11.84 MNNQAITTNFTTLSTVLWTGNGYY2174 pKa = 10.07 QKK2176 pKa = 10.51 VPSMCGAKK2184 pKa = 10.26 EE2185 pKa = 3.88 FVCTKK2190 pKa = 8.39 QHH2192 pKa = 3.86 NHH2194 pKa = 5.87 GYY2196 pKa = 9.71 FNCTMEE2202 pKa = 5.89 IKK2204 pKa = 10.42 DD2205 pKa = 3.4 DD2206 pKa = 3.6 WYY2208 pKa = 11.02 AHH2210 pKa = 5.47 VNEE2213 pKa = 4.57 CEE2215 pKa = 3.79 KK2216 pKa = 10.79 CKK2218 pKa = 10.32 RR2219 pKa = 11.84 YY2220 pKa = 9.57 YY2221 pKa = 9.52 QQNKK2225 pKa = 9.03 HH2226 pKa = 5.58 PRR2228 pKa = 11.84 CGAIYY2233 pKa = 9.6 SAEE2236 pKa = 4.17 TKK2238 pKa = 10.24 RR2239 pKa = 11.84 YY2240 pKa = 6.03 PTLANFINRR2249 pKa = 11.84 YY2250 pKa = 8.87 RR2251 pKa = 11.84 SCSHH2255 pKa = 7.07 CLPCTQCLSIRR2266 pKa = 11.84 EE2267 pKa = 4.27 ANCNIEE2273 pKa = 4.65 CYY2275 pKa = 10.26 HH2276 pKa = 6.78 PNDD2279 pKa = 3.32 NQIIQNQAFLTPVNVKK2295 pKa = 10.28 ADD2297 pKa = 3.81 SLVYY2301 pKa = 10.52 SYY2303 pKa = 11.72 VDD2305 pKa = 3.95 QEE2307 pKa = 4.29 NGDD2310 pKa = 3.45 INAVYY2315 pKa = 10.41 NGRR2318 pKa = 11.84 IWLMRR2323 pKa = 11.84 RR2324 pKa = 11.84 TNPIIPPPNRR2334 pKa = 11.84 YY2335 pKa = 9.28 RR2336 pKa = 11.84 LISNLKK2342 pKa = 8.63 VRR2344 pKa = 11.84 TYY2346 pKa = 11.03 DD2347 pKa = 3.3 PEE2349 pKa = 5.51 GYY2351 pKa = 10.55 YY2352 pKa = 10.47 YY2353 pKa = 10.07 ISDD2356 pKa = 3.93 VCPTDD2361 pKa = 4.98 LDD2363 pKa = 4.34 LLYY2366 pKa = 11.6 SMINQLQIKK2375 pKa = 8.53 LQEE2378 pKa = 3.9 RR2379 pKa = 11.84 TSIHH2383 pKa = 5.92 NEE2385 pKa = 3.77 AIIEE2389 pKa = 4.11 EE2390 pKa = 4.74 EE2391 pKa = 4.09 PTITPIVQQKK2401 pKa = 7.5 YY2402 pKa = 5.81 TAPLNGVMLSEE2413 pKa = 4.27 LRR2415 pKa = 11.84 EE2416 pKa = 3.82 KK2417 pKa = 10.58 HH2418 pKa = 6.72 RR2419 pKa = 11.84 YY2420 pKa = 8.84 LLLLKK2425 pKa = 10.3 IKK2427 pKa = 10.25 PNYY2430 pKa = 7.4 EE2431 pKa = 4.3 HH2432 pKa = 7.52 IYY2434 pKa = 10.28 HH2435 pKa = 6.46 EE2436 pKa = 4.65 LVHH2439 pKa = 6.22 EE2440 pKa = 4.46 LEE2442 pKa = 4.34 VLNLPIHH2449 pKa = 5.93 VCHH2452 pKa = 6.27 ITYY2455 pKa = 9.39 EE2456 pKa = 4.56 SPTLTNHH2463 pKa = 4.8 ATMYY2467 pKa = 10.58 INYY2470 pKa = 7.77 EE2471 pKa = 4.06 QVWRR2475 pKa = 11.84 EE2476 pKa = 4.37 KK2477 pKa = 9.69 ITGDD2481 pKa = 3.76 NIVKK2485 pKa = 10.04 ISKK2488 pKa = 9.8 HH2489 pKa = 4.68 IQSLITTPMDD2499 pKa = 3.53 FQHH2502 pKa = 6.44 GHH2504 pKa = 4.64 VQLSRR2509 pKa = 11.84 NSNVARR2515 pKa = 11.84 YY2516 pKa = 8.14 HH2517 pKa = 5.19 QLSTKK2522 pKa = 10.26 DD2523 pKa = 3.51 RR2524 pKa = 11.84 YY2525 pKa = 8.98 TIDD2528 pKa = 2.98 ISCNKK2533 pKa = 9.48 TSVTYY2538 pKa = 10.28 IPNGKK2543 pKa = 9.3 VINVKK2548 pKa = 10.07 IKK2550 pKa = 9.83 PLIEE2554 pKa = 3.89 KK2555 pKa = 9.6 EE2556 pKa = 3.71 YY2557 pKa = 10.47 RR2558 pKa = 11.84 IYY2560 pKa = 11.02 EE2561 pKa = 3.87 RR2562 pKa = 11.84 LYY2564 pKa = 10.53 NLNSEE2569 pKa = 4.61 LFLTKK2574 pKa = 9.27 HH2575 pKa = 5.35 TLKK2578 pKa = 10.59 EE2579 pKa = 4.3 HH2580 pKa = 6.21 PTTNYY2585 pKa = 10.82 LLRR2588 pKa = 11.84 HH2589 pKa = 6.54 DD2590 pKa = 4.53 LTALSLADD2598 pKa = 3.9 LYY2600 pKa = 11.69 GVVKK2604 pKa = 10.67 SEE2606 pKa = 3.68 NWKK2609 pKa = 9.61 PLYY2612 pKa = 8.97 DD2613 pKa = 3.87 TLPQITYY2620 pKa = 9.15 HH2621 pKa = 6.05 QIPKK2625 pKa = 10.17 EE2626 pKa = 3.77 LVLQIEE2632 pKa = 4.66 KK2633 pKa = 9.0 HH2634 pKa = 5.01 TSAPQHH2640 pKa = 5.32 TCCLLCRR2647 pKa = 11.84 RR2648 pKa = 11.84 FLAEE2652 pKa = 5.81 LGLLLHH2658 pKa = 6.82 KK2659 pKa = 10.52 LNKK2662 pKa = 9.65 QVFSTTTTILRR2673 pKa = 11.84 YY2674 pKa = 9.79 YY2675 pKa = 10.8 NFVLTSDD2682 pKa = 3.87 NVDD2685 pKa = 3.49 LNGILDD2691 pKa = 3.75 YY2692 pKa = 11.08 EE2693 pKa = 4.62 DD2694 pKa = 3.57 YY2695 pKa = 11.63 VEE2697 pKa = 5.41 IEE2699 pKa = 4.24 EE2700 pKa = 4.28 EE2701 pKa = 3.63 HH2702 pKa = 7.14 DD2703 pKa = 3.94 IEE2705 pKa = 5.41 IKK2707 pKa = 9.95 EE2708 pKa = 4.54 QLRR2711 pKa = 11.84 KK2712 pKa = 7.75 MQPYY2716 pKa = 8.81 YY2717 pKa = 9.26 HH2718 pKa = 6.76 TLYY2721 pKa = 10.64 SIYY2724 pKa = 9.99 EE2725 pKa = 3.92 HH2726 pKa = 6.37 TGMYY2730 pKa = 10.19 FISQPIYY2737 pKa = 10.58 DD2738 pKa = 3.92 RR2739 pKa = 11.84 AIEE2742 pKa = 4.02 NHH2744 pKa = 7.13 DD2745 pKa = 4.25 DD2746 pKa = 4.45 LIQRR2750 pKa = 11.84 YY2751 pKa = 8.59 EE2752 pKa = 3.9 EE2753 pKa = 4.12 VIEE2756 pKa = 4.37 NIRR2759 pKa = 11.84 TLTLDD2764 pKa = 3.61 SKK2766 pKa = 11.62 FEE2768 pKa = 4.08 DD2769 pKa = 3.57 TVLYY2773 pKa = 10.4 RR2774 pKa = 11.84 PTIQHH2779 pKa = 5.82 FAEE2782 pKa = 4.44 YY2783 pKa = 9.12 MKK2785 pKa = 10.58 LDD2787 pKa = 3.8 HH2788 pKa = 6.61 YY2789 pKa = 11.02 AVKK2792 pKa = 10.45 PEE2794 pKa = 4.32 PLWKK2798 pKa = 9.95 IYY2800 pKa = 9.13 DD2801 pKa = 4.04 TIKK2804 pKa = 10.59 SPKK2807 pKa = 9.57 IEE2809 pKa = 4.08 IPGIDD2814 pKa = 3.39 NVAIIIKK2821 pKa = 6.67 PTRR2824 pKa = 11.84 PFQDD2828 pKa = 3.65 YY2829 pKa = 11.26 VEE2831 pKa = 4.98 LNHH2834 pKa = 6.68 EE2835 pKa = 4.54 NIKK2838 pKa = 10.94 KK2839 pKa = 10.28 FDD2841 pKa = 3.26 GLTYY2845 pKa = 10.55 CKK2847 pKa = 9.95 INHH2850 pKa = 6.49 RR2851 pKa = 11.84 EE2852 pKa = 3.87 LYY2854 pKa = 10.1 NIQDD2858 pKa = 3.98 ILCNLPPEE2866 pKa = 4.24 ATIQEE2871 pKa = 4.89 LYY2873 pKa = 10.48 IVDD2876 pKa = 3.97 HH2877 pKa = 6.54 PFEE2880 pKa = 6.02 IEE2882 pKa = 4.39 SYY2884 pKa = 8.89 QHH2886 pKa = 5.19 MLKK2889 pKa = 10.71 VNLNIWLASMYY2900 pKa = 10.06 DD2901 pKa = 3.46 ANVNLSHH2908 pKa = 7.36 FDD2910 pKa = 3.56 SINYY2914 pKa = 7.9 EE2915 pKa = 3.79 RR2916 pKa = 11.84 TKK2918 pKa = 10.37 QVSFPVVGTLDD2929 pKa = 3.26 ATNLHH2934 pKa = 6.87 DD2935 pKa = 6.39 CEE2937 pKa = 4.67 ICQDD2941 pKa = 4.6 MIPPDD2946 pKa = 3.66 IKK2948 pKa = 10.6 SVYY2951 pKa = 10.37 DD2952 pKa = 3.41 IGSCTHH2958 pKa = 6.95 AKK2960 pKa = 10.16 AQLEE2964 pKa = 4.6 DD2965 pKa = 3.68 YY2966 pKa = 10.63 KK2967 pKa = 11.06 SPRR2970 pKa = 11.84 KK2971 pKa = 9.68 INPLVEE2977 pKa = 4.2 FDD2979 pKa = 3.66 TALLEE2984 pKa = 4.78 HH2985 pKa = 6.46 GTFTPEE2991 pKa = 3.04 NDD2993 pKa = 3.21 YY2994 pKa = 11.23 AYY2996 pKa = 9.96 TMKK2999 pKa = 10.39 TKK3001 pKa = 9.84 PDD3003 pKa = 3.58 HH3004 pKa = 7.24 LIDD3007 pKa = 5.3 LEE3009 pKa = 4.42 LKK3011 pKa = 10.64 SYY3013 pKa = 10.81 LDD3015 pKa = 3.61 KK3016 pKa = 11.52 SGLTALIPPLNPNPIIHH3033 pKa = 7.34 DD3034 pKa = 3.85 PDD3036 pKa = 3.49 KK3037 pKa = 10.3 TFSSAYY3043 pKa = 9.32 YY3044 pKa = 9.8 IKK3046 pKa = 9.71 TPSEE3050 pKa = 3.71 ASIQEE3055 pKa = 4.1 DD3056 pKa = 4.05 LEE3058 pKa = 4.32 MFNQNTAAAVSPTVYY3073 pKa = 10.91 LMATEE3078 pKa = 5.19 LLHH3081 pKa = 6.86 QLLTEE3086 pKa = 4.26 TVLASDD3092 pKa = 4.25 GNKK3095 pKa = 9.23 NCPMRR3100 pKa = 11.84 PSDD3103 pKa = 3.53 VPIRR3107 pKa = 11.84 NKK3109 pKa = 10.15 HH3110 pKa = 5.62 KK3111 pKa = 10.97 SSGTPYY3117 pKa = 10.3 RR3118 pKa = 11.84 KK3119 pKa = 10.37 YY3120 pKa = 10.79 GDD3122 pKa = 3.75 SEE3124 pKa = 4.36 FMRR3127 pKa = 11.84 EE3128 pKa = 3.9 LYY3130 pKa = 10.75 GEE3132 pKa = 4.21 HH3133 pKa = 7.15 RR3134 pKa = 11.84 DD3135 pKa = 3.26 ALVYY3139 pKa = 10.23 HH3140 pKa = 6.73 KK3141 pKa = 10.48 RR3142 pKa = 11.84 HH3143 pKa = 6.13 SADD3146 pKa = 3.02 QALTLVINKK3155 pKa = 8.78 VAISTKK3161 pKa = 10.18 HH3162 pKa = 6.23 RR3163 pKa = 11.84 DD3164 pKa = 3.37 RR3165 pKa = 11.84 TILAININKK3174 pKa = 9.69 SEE3176 pKa = 4.21 AGRR3179 pKa = 11.84 SLFRR3183 pKa = 11.84 WFLDD3187 pKa = 3.64 KK3188 pKa = 10.68 IKK3190 pKa = 10.11 YY3191 pKa = 5.79 TANTGGPILIGLIAQYY3207 pKa = 11.0 GGWDD3211 pKa = 3.27 KK3212 pKa = 11.21 LYY3214 pKa = 11.14 KK3215 pKa = 10.18 NLYY3218 pKa = 8.93 KK3219 pKa = 10.67 DD3220 pKa = 3.82 SPAEE3224 pKa = 4.02 NPEE3227 pKa = 4.2 TAPHH3231 pKa = 5.79 VLLGGKK3237 pKa = 9.44 DD3238 pKa = 3.5 YY3239 pKa = 10.86 PKK3241 pKa = 9.35 WDD3243 pKa = 3.39 RR3244 pKa = 11.84 RR3245 pKa = 11.84 ISNMLQLTTTNIFFNMIDD3263 pKa = 3.34 QLQQYY3268 pKa = 6.99 QHH3270 pKa = 6.31 NNATPSEE3277 pKa = 4.26 TWHH3280 pKa = 6.68 EE3281 pKa = 4.27 YY3282 pKa = 9.68 MSEE3285 pKa = 4.11 TTQILYY3291 pKa = 10.47 DD3292 pKa = 3.62 YY3293 pKa = 10.96 LVFGNKK3299 pKa = 9.52 LYY3301 pKa = 10.43 QKK3303 pKa = 10.01 PGGVTSGNSRR3313 pKa = 11.84 TADD3316 pKa = 3.25 GNSFLHH3322 pKa = 6.45 LLIDD3326 pKa = 3.94 MYY3328 pKa = 11.79 ALIVQLIQSVPEE3340 pKa = 4.02 NVDD3343 pKa = 2.52 IEE3345 pKa = 4.42 APLRR3349 pKa = 11.84 SQLCKK3354 pKa = 9.9 IALTVIPSDD3363 pKa = 3.79 YY3364 pKa = 11.39 LEE3366 pKa = 5.03 DD3367 pKa = 4.03 PQLRR3371 pKa = 11.84 STDD3374 pKa = 2.98 ILTSIRR3380 pKa = 11.84 TRR3382 pKa = 11.84 VAKK3385 pKa = 10.12 GAYY3388 pKa = 9.98 LSDD3391 pKa = 4.96 DD3392 pKa = 3.68 GLIVIDD3398 pKa = 4.09 TRR3400 pKa = 11.84 LIRR3403 pKa = 11.84 YY3404 pKa = 9.21 DD3405 pKa = 3.68 DD3406 pKa = 4.4 FMAEE3410 pKa = 3.53 SHH3412 pKa = 6.81 MISKK3416 pKa = 10.25 YY3417 pKa = 10.61 LIANNKK3423 pKa = 8.35 HH3424 pKa = 6.43 KK3425 pKa = 11.23 YY3426 pKa = 10.01 HH3427 pKa = 6.9 MDD3429 pKa = 5.09 PIQRR3433 pKa = 11.84 KK3434 pKa = 8.34 ARR3436 pKa = 11.84 EE3437 pKa = 4.18 FLSQDD3442 pKa = 2.99 TFDD3445 pKa = 4.94 FGDD3448 pKa = 3.33 MSYY3451 pKa = 10.79 PLPEE3455 pKa = 4.35 FGRR3458 pKa = 11.84 IYY3460 pKa = 10.86 SAMLLSDD3467 pKa = 4.37 NKK3469 pKa = 9.68 NTLDD3473 pKa = 3.5 PQINITRR3480 pKa = 11.84 LLSLYY3485 pKa = 10.0 AYY3487 pKa = 10.51 LYY3489 pKa = 10.07 IYY3491 pKa = 8.87 YY3492 pKa = 9.92 FKK3494 pKa = 10.92 YY3495 pKa = 10.78 AEE3497 pKa = 4.42 QPTQNYY3503 pKa = 9.23 IKK3505 pKa = 10.67 LLDD3508 pKa = 4.83 AIRR3511 pKa = 11.84 KK3512 pKa = 9.34 YY3513 pKa = 10.3 IDD3515 pKa = 3.26 EE3516 pKa = 4.33 RR3517 pKa = 11.84 LEE3519 pKa = 4.02 TTEE3522 pKa = 5.72 DD3523 pKa = 3.41 QFLDD3527 pKa = 4.49 CIKK3530 pKa = 10.57 IPDD3533 pKa = 4.06 LQDD3536 pKa = 3.16 VEE3538 pKa = 5.85 LDD3540 pKa = 4.13 LKK3542 pKa = 10.68 NCDD3545 pKa = 2.99 MFEE3548 pKa = 4.47 NFDD3551 pKa = 4.2 HH3552 pKa = 7.06 LWGFDD3557 pKa = 3.36 LSDD3560 pKa = 3.63 AYY3562 pKa = 9.87 IEE3564 pKa = 4.16 YY3565 pKa = 9.74 LHH3567 pKa = 6.77 KK3568 pKa = 10.86 YY3569 pKa = 6.99 KK3570 pKa = 10.48 HH3571 pKa = 6.7 RR3572 pKa = 11.84 YY3573 pKa = 7.18 PKK3575 pKa = 10.56 LSIFKK3580 pKa = 10.31 RR3581 pKa = 11.84 QILEE3585 pKa = 3.83 QYY3587 pKa = 9.23 EE3588 pKa = 4.07 QAKK3591 pKa = 9.98 LVNKK3595 pKa = 10.32 NILHH3599 pKa = 6.07 NKK3601 pKa = 6.59 GTYY3604 pKa = 7.54 VTYY3607 pKa = 10.41 NCYY3610 pKa = 10.17 VCGEE3614 pKa = 4.24 NAHH3617 pKa = 6.06 ITCALCEE3624 pKa = 3.81 RR3625 pKa = 11.84 VFCNSKK3631 pKa = 10.85 DD3632 pKa = 3.75 STHH3635 pKa = 6.4 GSHH3638 pKa = 7.21 IEE3640 pKa = 3.63 QHH3642 pKa = 5.63 LQYY3645 pKa = 10.46 SGHH3648 pKa = 6.12 NSLYY3652 pKa = 10.73 LNCKK3656 pKa = 6.33 TVKK3659 pKa = 9.94 CRR3661 pKa = 11.84 HH3662 pKa = 6.39 CYY3664 pKa = 8.48 VTDD3667 pKa = 3.39 INNLYY3672 pKa = 8.87 TAGKK3676 pKa = 9.99 DD3677 pKa = 3.06 MYY3679 pKa = 9.97 YY3680 pKa = 10.75 CLVHH3684 pKa = 7.12 KK3685 pKa = 7.67 PTQSVKK3691 pKa = 10.8 LLNNNVNEE3699 pKa = 4.09 KK3700 pKa = 10.58 LPPNLYY3706 pKa = 10.66 LCITDD3711 pKa = 3.49 SRR3713 pKa = 11.84 PVPFYY3718 pKa = 10.11 EE3719 pKa = 3.91 RR3720 pKa = 11.84 CYY3722 pKa = 10.41 EE3723 pKa = 4.16 NYY3725 pKa = 9.25 TAIHH3729 pKa = 7.0 PDD3731 pKa = 3.36 YY3732 pKa = 10.57 PVTYY3736 pKa = 9.52 NNFMSLIQQYY3746 pKa = 10.67 LYY3748 pKa = 10.85 QDD3750 pKa = 3.23 YY3751 pKa = 8.6 TLPQNQLANRR3761 pKa = 11.84 IRR3763 pKa = 11.84 VRR3765 pKa = 11.84 LQLSSYY3771 pKa = 10.28 GIVRR3775 pKa = 11.84 PYY3777 pKa = 10.04 HH3778 pKa = 4.79 QLIMQLTKK3786 pKa = 10.2 IEE3788 pKa = 4.48 SKK3790 pKa = 11.09 VIDD3793 pKa = 4.27 ASVVDD3798 pKa = 4.48 IPITPTNSLEE3808 pKa = 3.98 IGTCYY3813 pKa = 10.26 IEE3815 pKa = 4.43 VPQVTKK3821 pKa = 10.81 LDD3823 pKa = 3.51 QHH3825 pKa = 5.3 STYY3828 pKa = 11.04 SYY3830 pKa = 10.92 LLGNRR3835 pKa = 11.84 EE3836 pKa = 4.28 VNFTPTYY3843 pKa = 10.35 FRR3845 pKa = 11.84 LSNKK3849 pKa = 7.18 TTHH3852 pKa = 5.88 IWQTDD3857 pKa = 3.5 VQIPTSCTYY3866 pKa = 10.01 IRR3868 pKa = 11.84 QRR3870 pKa = 11.84 RR3871 pKa = 11.84 LNTLSAILRR3880 pKa = 11.84 NTTQHH3885 pKa = 5.31 VPEE3888 pKa = 4.71 FTRR3891 pKa = 11.84 LLMSWNQQLPIAARR3905 pKa = 11.84 PFIEE3909 pKa = 5.41 FIPTFRR3915 pKa = 11.84 IPAQPTVAEE3924 pKa = 4.8 NINILLNEE3932 pKa = 4.28 LNVKK3936 pKa = 9.81 RR3937 pKa = 11.84 FKK3939 pKa = 11.06 IMFGGPGTGKK3949 pKa = 9.81 SHH3951 pKa = 6.19 TLSILVNHH3959 pKa = 6.34 LHH3961 pKa = 6.29 EE3962 pKa = 5.44 KK3963 pKa = 9.97 GFKK3966 pKa = 9.73 ILIYY3970 pKa = 9.97 TPSHH3974 pKa = 5.73 QSANALLHH3982 pKa = 6.34 KK3983 pKa = 9.76 IASIMRR3989 pKa = 11.84 KK3990 pKa = 9.65 RR3991 pKa = 11.84 NIQNPGLTRR4000 pKa = 11.84 IVTDD4004 pKa = 3.64 GMKK4007 pKa = 10.64 EE4008 pKa = 3.86 EE4009 pKa = 5.07 LKK4011 pKa = 9.87 PNPYY4015 pKa = 7.57 ITYY4018 pKa = 9.38 RR4019 pKa = 11.84 ASMLDD4024 pKa = 3.11 KK4025 pKa = 11.1 DD4026 pKa = 5.01 RR4027 pKa = 11.84 ICVTTIQSFSTVQHH4041 pKa = 5.95 VKK4043 pKa = 10.75 DD4044 pKa = 3.74 VDD4046 pKa = 4.29 LVILDD4051 pKa = 4.22 EE4052 pKa = 5.43 FSLTSDD4058 pKa = 3.29 NYY4060 pKa = 11.07 LLTGLSHH4067 pKa = 7.31 LKK4069 pKa = 10.27 PSTRR4073 pKa = 11.84 VLFSGDD4079 pKa = 3.25 PRR4081 pKa = 11.84 QLSGVDD4087 pKa = 3.42 EE4088 pKa = 4.34 TRR4090 pKa = 11.84 KK4091 pKa = 7.55 TLKK4094 pKa = 10.43 PCFHH4098 pKa = 6.67 TLINYY4103 pKa = 6.66 YY4104 pKa = 8.73 TEE4106 pKa = 4.62 IYY4108 pKa = 9.05 PKK4110 pKa = 9.77 EE4111 pKa = 4.04 VHH4113 pKa = 5.08 VLKK4116 pKa = 10.99 YY4117 pKa = 10.26 HH4118 pKa = 6.11 FRR4120 pKa = 11.84 CHH4122 pKa = 6.21 PLIFKK4127 pKa = 9.3 YY4128 pKa = 10.58 FKK4130 pKa = 10.19 DD4131 pKa = 3.06 QYY4133 pKa = 11.15 YY4134 pKa = 10.63 ADD4136 pKa = 4.89 KK4137 pKa = 11.4 DD4138 pKa = 4.12 MEE4140 pKa = 4.77 CKK4142 pKa = 9.64 TLAEE4146 pKa = 4.27 DD4147 pKa = 5.3 RR4148 pKa = 11.84 IIRR4151 pKa = 11.84 PLKK4154 pKa = 9.89 PISTVKK4160 pKa = 10.55 ILDD4163 pKa = 3.48 PHH4165 pKa = 6.33 YY4166 pKa = 10.91 KK4167 pKa = 10.22 NLGVVLNQDD4176 pKa = 3.34 EE4177 pKa = 4.58 TDD4179 pKa = 3.37 KK4180 pKa = 11.6 VLDD4183 pKa = 3.52 ILVLITQTISLHH4195 pKa = 5.88 ASYY4198 pKa = 10.58 EE4199 pKa = 4.19 FQPTTAIICSYY4210 pKa = 10.75 KK4211 pKa = 10.6 SQLQNFQALQQQKK4224 pKa = 9.94 ILPEE4228 pKa = 3.98 YY4229 pKa = 10.32 VKK4231 pKa = 11.04 LSTIDD4236 pKa = 3.32 SAQGDD4241 pKa = 3.95 EE4242 pKa = 4.13 FDD4244 pKa = 5.47 IVILCLSQINNFTLNPNRR4262 pKa = 11.84 FNVAISRR4269 pKa = 11.84 AKK4271 pKa = 9.6 SVLFITVPPIDD4282 pKa = 4.37 KK4283 pKa = 10.68 SPHH4286 pKa = 4.54 FQYY4289 pKa = 11.25 KK4290 pKa = 8.8 DD4291 pKa = 3.21 TYY4293 pKa = 10.46 NKK4295 pKa = 10.44 LLEE4298 pKa = 4.42 TEE4300 pKa = 3.7 QFEE4303 pKa = 4.77 YY4304 pKa = 11.0 YY4305 pKa = 10.34 KK4306 pKa = 10.18 IYY4308 pKa = 11.03 NEE4310 pKa = 4.29 GSSKK4314 pKa = 10.41 ILLLDD4319 pKa = 3.67 SPTTLRR4325 pKa = 11.84 NKK4327 pKa = 9.37 ATFINIRR4334 pKa = 11.84 NLAGDD4339 pKa = 3.56 THH4341 pKa = 7.22 KK4342 pKa = 11.11 LQTTFPINIVMSDD4355 pKa = 3.58 YY4356 pKa = 11.2 IFFDD4360 pKa = 4.12 SEE4362 pKa = 4.09 FLNPLEE4368 pKa = 4.28 NRR4370 pKa = 11.84 QEE4372 pKa = 4.09 PVMLSYY4378 pKa = 11.47 GFTHH4382 pKa = 6.91 EE4383 pKa = 4.05 NNKK4386 pKa = 9.53 RR4387 pKa = 11.84 RR4388 pKa = 11.84 IAGIPMRR4395 pKa = 11.84 YY4396 pKa = 9.18 VKK4398 pKa = 10.85 NKK4400 pKa = 10.01 FNILTPIQYY4409 pKa = 10.54 NFVDD4413 pKa = 3.72 NDD4415 pKa = 3.45 NPLTSTYY4422 pKa = 10.25 SCNWLKK4428 pKa = 10.97 HH4429 pKa = 5.97 KK4430 pKa = 10.76 DD4431 pKa = 3.46 PSQFRR4436 pKa = 11.84 HH4437 pKa = 5.63 LSFSVKK4443 pKa = 10.49 VGIKK4447 pKa = 10.7 NNTTVEE4453 pKa = 4.06 LRR4455 pKa = 11.84 HH4456 pKa = 5.66 MLNYY4460 pKa = 10.62 CIDD4463 pKa = 3.74 NTHH4466 pKa = 5.82 VKK4468 pKa = 9.86 PVIVTWAGASDD4479 pKa = 3.71 HH4480 pKa = 6.88 CFLKK4484 pKa = 11.03 ANTLYY4489 pKa = 10.44 PPISQMCNVTIKK4501 pKa = 10.57 CKK4503 pKa = 10.56 SPPIYY4508 pKa = 10.45 ASPQGCHH4515 pKa = 5.7 TYY4517 pKa = 10.3 FLCSHH4522 pKa = 7.34 HH4523 pKa = 6.83 AQTLKK4528 pKa = 10.23 EE4529 pKa = 4.26 QINITHH4535 pKa = 6.57 FVNLEE4540 pKa = 4.02 IIDD4543 pKa = 4.09 LKK4545 pKa = 10.76 VEE4547 pKa = 4.36 KK4548 pKa = 10.25 NVKK4551 pKa = 9.03 TNEE4554 pKa = 3.6 TTMNVIHH4561 pKa = 6.64 NGNHH4565 pKa = 6.77 KK4566 pKa = 9.38 LTIGLQNLTSNSLKK4580 pKa = 10.46 DD4581 pKa = 3.39 CHH4583 pKa = 7.06 AFYY4586 pKa = 10.94 CNTRR4590 pKa = 11.84 HH4591 pKa = 6.73 APITPHH4597 pKa = 7.48 DD4598 pKa = 4.54 PLDD4601 pKa = 4.24 DD4602 pKa = 4.38 ALMTQCLFHH4611 pKa = 7.87 SIVRR4615 pKa = 11.84 TKK4617 pKa = 10.68 FEE4619 pKa = 4.56 EE4620 pKa = 3.83 IVYY4623 pKa = 9.78 EE4624 pKa = 4.28 EE4625 pKa = 4.44 KK4626 pKa = 11.3 ANVAAFISMDD4636 pKa = 3.15 YY4637 pKa = 10.6 RR4638 pKa = 11.84 LRR4640 pKa = 11.84 NFNPEE4645 pKa = 3.38 MCKK4648 pKa = 10.11 LRR4650 pKa = 11.84 RR4651 pKa = 11.84 EE4652 pKa = 4.17 LQEE4655 pKa = 3.64 YY4656 pKa = 8.65 WYY4658 pKa = 10.06 KK4659 pKa = 10.74 KK4660 pKa = 10.32 YY4661 pKa = 10.91 SATTKK4666 pKa = 6.82 THH4668 pKa = 6.76 CNMGCGKK4675 pKa = 10.38 DD4676 pKa = 3.58 KK4677 pKa = 10.17 LTHH4680 pKa = 6.41 ALHH4683 pKa = 6.92 NIDD4686 pKa = 4.06 ILQGKK4691 pKa = 9.18 DD4692 pKa = 3.15 PQNNMNTHH4700 pKa = 5.64 TCNSEE4705 pKa = 3.39 EE4706 pKa = 4.18 HH4707 pKa = 6.97 IYY4709 pKa = 10.67 FDD4711 pKa = 3.65 SHH4713 pKa = 5.93 WYY4715 pKa = 8.22 KK4716 pKa = 10.31 TDD4718 pKa = 3.19 SFKK4721 pKa = 11.1 KK4722 pKa = 9.32 PSYY4725 pKa = 10.3 IFSDD4729 pKa = 3.44 INNDD4733 pKa = 2.72 HH4734 pKa = 6.8 YY4735 pKa = 11.94 YY4736 pKa = 11.27 NLGTTGLSLYY4746 pKa = 10.69 LNSRR4750 pKa = 11.84 LAKK4753 pKa = 10.13 YY4754 pKa = 8.92 IHH4756 pKa = 6.94 CFNPVQGTDD4765 pKa = 3.11 VFKK4768 pKa = 11.01 TEE4770 pKa = 5.57 FYY4772 pKa = 11.02 DD4773 pKa = 3.99 STCSEE4778 pKa = 3.94 TRR4780 pKa = 11.84 EE4781 pKa = 4.18 TEE4783 pKa = 3.77 NFVIKK4788 pKa = 9.69 DD4789 pKa = 3.45 TTSIPDD4795 pKa = 4.19 CIIHH4799 pKa = 6.74 SSTDD4803 pKa = 2.73 ACAQNFKK4810 pKa = 11.28 SFVCTNHH4817 pKa = 6.67 IGQIDD4822 pKa = 4.6 IISKK4826 pKa = 9.94 ISQATHH4832 pKa = 5.54 YY4833 pKa = 9.62 GYY4835 pKa = 11.15 SFVKK4839 pKa = 8.71 VTPTTIHH4846 pKa = 5.7 NQSNLKK4852 pKa = 9.88 RR4853 pKa = 11.84 APPTWDD4859 pKa = 3.06 HH4860 pKa = 5.92 TTLQIPGYY4868 pKa = 8.68 EE4869 pKa = 3.57 IRR4871 pKa = 11.84 TPQSVGHH4878 pKa = 5.61 MQMKK4882 pKa = 10.46 ALGILSMLQDD4892 pKa = 3.39 SMLFTTKK4899 pKa = 9.98 TKK4901 pKa = 10.61 LKK4903 pKa = 9.2 PNMPIIHH4910 pKa = 7.18 PGAAGHH4916 pKa = 6.08 YY4917 pKa = 8.78 GQTALANEE4925 pKa = 4.76 FKK4927 pKa = 10.81 KK4928 pKa = 10.16 HH4929 pKa = 5.71 LKK4931 pKa = 9.08 QNHH4934 pKa = 7.04 IINIDD4939 pKa = 3.39 PRR4941 pKa = 11.84 LKK4943 pKa = 10.61 QEE4945 pKa = 3.55 NTTNFKK4951 pKa = 9.2 KK4952 pKa = 9.13 TLKK4955 pKa = 10.33 EE4956 pKa = 3.85 MLNIGYY4962 pKa = 6.9 PTEE4965 pKa = 4.86 IIISDD4970 pKa = 2.93 IHH4972 pKa = 6.11 NNKK4975 pKa = 9.3 VEE4977 pKa = 4.56 WITEE4981 pKa = 3.86 LCEE4984 pKa = 3.96 YY4985 pKa = 8.61 TNKK4988 pKa = 10.43 YY4989 pKa = 10.29 LLEE4992 pKa = 4.41 TGTLIFKK4999 pKa = 8.72 ITSRR5003 pKa = 11.84 GATEE5007 pKa = 3.63 QALEE5011 pKa = 4.09 LLQKK5015 pKa = 10.72 LSEE5018 pKa = 4.16 QFTYY5022 pKa = 10.37 MRR5024 pKa = 11.84 VCNLNAVTRR5033 pKa = 11.84 SSEE5036 pKa = 3.91 LWVVFANKK5044 pKa = 9.96 RR5045 pKa = 11.84 KK5046 pKa = 8.55 PTVQGWMPHH5055 pKa = 4.88 EE5056 pKa = 4.33 LRR5058 pKa = 11.84 NEE5060 pKa = 3.97 LRR5062 pKa = 11.84 KK5063 pKa = 9.46 HH5064 pKa = 5.39 WYY5066 pKa = 10.96 SMTQNIITPIQRR5078 pKa = 11.84 HH5079 pKa = 5.57 RR5080 pKa = 11.84 IGTFGYY5086 pKa = 9.88 SPKK5089 pKa = 10.89
Molecular weight: 590.66 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 6.708
IPC2_protein 6.912
IPC_protein 6.795
Toseland 6.81
ProMoST 7.38
Dawson 7.38
Bjellqvist 7.673
Wikipedia 7.337
Rodwell 7.395
Grimsley 6.942
Solomon 7.38
Lehninger 7.395
Nozaki 7.878
DTASelect 7.658
Thurlkill 7.702
EMBOSS 7.688
Sillero 7.878
Patrickios 3.63
IPC_peptide 7.38
IPC2_peptide 7.176
IPC2.peptide.svr19 6.971
Protein with the highest isoelectric point:
>tr|A0A1B1X3Z9|A0A1B1X3Z9_9NIDO Putative membrane protein OS=Kadiweu virus OX=1795438 PE=4 SV=1
MM1 pKa = 7.5 SANASNNTASTSTTKK16 pKa = 10.44 PKK18 pKa = 9.34 QQRR21 pKa = 11.84 QNQNQVQQNNTPKK34 pKa = 10.3 PQRR37 pKa = 11.84 QSKK40 pKa = 8.58 NQQNQQKK47 pKa = 10.66 APLRR51 pKa = 11.84 AQNQNAAPRR60 pKa = 11.84 KK61 pKa = 8.18 NNQRR65 pKa = 11.84 KK66 pKa = 7.33 ATNAGSQPKK75 pKa = 10.26 APAGKK80 pKa = 8.41 PKK82 pKa = 10.53 KK83 pKa = 9.82 PIAVGPNYY91 pKa = 10.13 TDD93 pKa = 3.48 TNGKK97 pKa = 9.01 RR98 pKa = 11.84 YY99 pKa = 10.17 KK100 pKa = 10.08 IGRR103 pKa = 11.84 EE104 pKa = 3.37 FDD106 pKa = 3.35 ARR108 pKa = 11.84 NHH110 pKa = 5.43 MGWRR114 pKa = 11.84 RR115 pKa = 11.84 NEE117 pKa = 4.03 KK118 pKa = 9.59 TGSTVQFLFKK128 pKa = 10.41 PKK130 pKa = 8.74 MASRR134 pKa = 11.84 VDD136 pKa = 3.23 QAYY139 pKa = 10.17 YY140 pKa = 10.48 RR141 pKa = 11.84 SQFEE145 pKa = 4.67 DD146 pKa = 3.18 SDD148 pKa = 4.53 HH149 pKa = 6.58 YY150 pKa = 11.29 IHH152 pKa = 6.75 TFGVGVFVQDD162 pKa = 4.01 SNMDD166 pKa = 3.44 RR167 pKa = 11.84 NAVLRR172 pKa = 11.84 PQDD175 pKa = 3.54 LSEE178 pKa = 5.0 KK179 pKa = 10.05 EE180 pKa = 3.99 KK181 pKa = 10.73 QDD183 pKa = 3.65 YY184 pKa = 11.12 LEE186 pKa = 4.39 NLANAFDD193 pKa = 5.11 KK194 pKa = 11.04 ILVRR198 pKa = 11.84 TKK200 pKa = 10.39 FAFEE204 pKa = 4.6 NNSQPLLVIDD214 pKa = 4.38 AA215 pKa = 4.8
Molecular weight: 24.45 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.172
IPC2_protein 9.545
IPC_protein 9.633
Toseland 10.482
ProMoST 10.014
Dawson 10.584
Bjellqvist 10.189
Wikipedia 10.716
Rodwell 11.125
Grimsley 10.628
Solomon 10.613
Lehninger 10.599
Nozaki 10.438
DTASelect 10.189
Thurlkill 10.467
EMBOSS 10.847
Sillero 10.496
Patrickios 10.847
IPC_peptide 10.628
IPC2_peptide 8.624
IPC2.peptide.svr19 8.697
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
3
1
4
7967
157
5089
1991.8
230.91
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
4.732 ± 1.151
2.197 ± 0.331
5.071 ± 0.122
5.134 ± 0.733
4.18 ± 0.238
3.063 ± 0.218
3.514 ± 0.479
7.807 ± 1.167
6.652 ± 0.43
9.401 ± 0.903
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.347 ± 0.172
7.167 ± 0.615
4.77 ± 0.33
4.406 ± 1.286
3.941 ± 0.475
5.272 ± 0.881
8.447 ± 0.611
5.096 ± 0.182
0.602 ± 0.08
6.201 ± 0.525
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here