Stx2-converting phage 86

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Podoviridae; Sepvirinae; Traversvirus; Escherichia virus 86

Average proteome isoelectric point is 7.03

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 81 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|Q08J30|Q08J30_9CAUD Protein ninE OS=Stx2-converting phage 86 OX=379329 GN=orf75 PE=3 SV=1
MM1 pKa = 7.82IDD3 pKa = 3.4PNRR6 pKa = 11.84SYY8 pKa = 10.61EE9 pKa = 3.97QEE11 pKa = 4.12SVEE14 pKa = 5.1RR15 pKa = 11.84ALTCANCGQKK25 pKa = 10.32LHH27 pKa = 5.86VLEE30 pKa = 4.31VHH32 pKa = 6.17VCSDD36 pKa = 4.12CCAEE40 pKa = 5.29LMSDD44 pKa = 4.35PNSSMYY50 pKa = 10.6EE51 pKa = 3.98EE52 pKa = 5.0EE53 pKa = 4.99DD54 pKa = 4.39DD55 pKa = 4.1EE56 pKa = 4.75

Molecular weight: 6.35 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|Q08J48|Q08J48_9CAUD Uncharacterized protein orf57 OS=Stx2-converting phage 86 OX=379329 GN=orf57 PE=4 SV=1
MM1 pKa = 7.26TRR3 pKa = 11.84RR4 pKa = 11.84TQFKK8 pKa = 9.33GNSRR12 pKa = 11.84SRR14 pKa = 11.84RR15 pKa = 11.84RR16 pKa = 11.84EE17 pKa = 3.62RR18 pKa = 11.84LKK20 pKa = 11.17AKK22 pKa = 10.38ALANGVLARR31 pKa = 11.84EE32 pKa = 4.21EE33 pKa = 4.75AISSEE38 pKa = 4.23VLHH41 pKa = 6.83RR42 pKa = 11.84PTLSRR47 pKa = 11.84AQIQAKK53 pKa = 8.11GTHH56 pKa = 5.29EE57 pKa = 4.03TPEE60 pKa = 4.7RR61 pKa = 11.84IEE63 pKa = 3.89DD64 pKa = 3.57AKK66 pKa = 10.63PIKK69 pKa = 10.49FMAQDD74 pKa = 3.8VIWQQKK80 pKa = 6.5EE81 pKa = 3.56YY82 pKa = 10.85RR83 pKa = 11.84RR84 pKa = 11.84NLEE87 pKa = 3.51RR88 pKa = 11.84AAIVYY93 pKa = 10.58ANEE96 pKa = 4.32FGHH99 pKa = 6.02KK100 pKa = 9.59QPEE103 pKa = 4.48TGVCLPNVALYY114 pKa = 10.32AAGYY118 pKa = 9.13RR119 pKa = 11.84KK120 pKa = 9.93SKK122 pKa = 10.61QLTARR127 pKa = 3.76

Molecular weight: 14.59 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

80

1

81

18160

48

2783

224.2

24.99

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

9.119 ± 0.321

1.206 ± 0.214

5.688 ± 0.235

6.729 ± 0.269

3.216 ± 0.167

7.346 ± 0.603

1.812 ± 0.154

5.088 ± 0.329

5.997 ± 0.275

7.83 ± 0.205

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.819 ± 0.145

4.471 ± 0.146

4.499 ± 0.289

4.752 ± 0.294

6.448 ± 0.305

6.382 ± 0.236

5.655 ± 0.245

6.399 ± 0.258

1.448 ± 0.151

3.095 ± 0.182
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski