Amino acid dipepetide frequency for Ralstonia phage RSB2

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10-3.

For more information see sequence space article on Wikipedia.

AlaCysAspGluPheGlyHisIleLysLeuMetAsnProGlnArgSerThrValTrpTyrXaa
Ala
13.381AlaAla: 13.381 ± 1.421
0.985AlaCys: 0.985 ± 0.279
6.321AlaAsp: 6.321 ± 0.754
6.732AlaGlu: 6.732 ± 0.655
3.941AlaPhe: 3.941 ± 0.621
8.456AlaGly: 8.456 ± 0.82
1.478AlaHis: 1.478 ± 0.258
5.5AlaIle: 5.5 ± 0.594
6.239AlaLys: 6.239 ± 0.788
9.851AlaLeu: 9.851 ± 0.939
2.381AlaMet: 2.381 ± 0.474
3.941AlaAsn: 3.941 ± 0.414
4.187AlaPro: 4.187 ± 0.879
5.5AlaGln: 5.5 ± 0.753
7.06AlaArg: 7.06 ± 0.81
5.254AlaSer: 5.254 ± 0.491
6.321AlaThr: 6.321 ± 1.205
7.306AlaVal: 7.306 ± 0.781
1.478AlaTrp: 1.478 ± 0.414
2.545AlaTyr: 2.545 ± 0.363
0.0AlaXaa: 0.0 ± 0.0
Cys
1.067CysAla: 1.067 ± 0.431
0.082CysCys: 0.082 ± 0.082
0.903CysAsp: 0.903 ± 0.308
0.739CysGlu: 0.739 ± 0.277
0.575CysPhe: 0.575 ± 0.251
0.575CysGly: 0.575 ± 0.223
0.164CysHis: 0.164 ± 0.11
0.41CysIle: 0.41 ± 0.194
0.575CysLys: 0.575 ± 0.225
0.903CysLeu: 0.903 ± 0.298
0.164CysMet: 0.164 ± 0.125
0.164CysAsn: 0.164 ± 0.115
0.493CysPro: 0.493 ± 0.221
0.164CysGln: 0.164 ± 0.105
0.657CysArg: 0.657 ± 0.258
0.657CysSer: 0.657 ± 0.217
0.246CysThr: 0.246 ± 0.126
0.575CysVal: 0.575 ± 0.223
0.164CysTrp: 0.164 ± 0.121
0.246CysTyr: 0.246 ± 0.141
0.0CysXaa: 0.0 ± 0.0
Asp
6.321AspAla: 6.321 ± 0.801
0.739AspCys: 0.739 ± 0.257
3.448AspAsp: 3.448 ± 0.54
4.269AspGlu: 4.269 ± 0.621
2.955AspPhe: 2.955 ± 0.444
5.582AspGly: 5.582 ± 0.54
0.985AspHis: 0.985 ± 0.333
3.038AspIle: 3.038 ± 0.523
2.709AspLys: 2.709 ± 0.496
5.665AspLeu: 5.665 ± 0.513
0.903AspMet: 0.903 ± 0.278
2.134AspAsn: 2.134 ± 0.341
3.858AspPro: 3.858 ± 0.679
2.217AspGln: 2.217 ± 0.389
2.955AspArg: 2.955 ± 0.33
2.627AspSer: 2.627 ± 0.372
4.269AspThr: 4.269 ± 0.55
4.187AspVal: 4.187 ± 0.502
0.903AspTrp: 0.903 ± 0.258
2.134AspTyr: 2.134 ± 0.315
0.0AspXaa: 0.0 ± 0.0
Glu
7.306GluAla: 7.306 ± 0.659
0.821GluCys: 0.821 ± 0.323
4.105GluAsp: 4.105 ± 0.637
4.023GluGlu: 4.023 ± 0.556
2.791GluPhe: 2.791 ± 0.483
5.911GluGly: 5.911 ± 0.833
1.149GluHis: 1.149 ± 0.411
2.052GluIle: 2.052 ± 0.395
2.381GluLys: 2.381 ± 0.456
4.269GluLeu: 4.269 ± 0.496
1.314GluMet: 1.314 ± 0.242
2.545GluAsn: 2.545 ± 0.437
2.052GluPro: 2.052 ± 0.447
2.955GluGln: 2.955 ± 0.545
4.762GluArg: 4.762 ± 0.741
2.791GluSer: 2.791 ± 0.416
3.694GluThr: 3.694 ± 0.467
4.433GluVal: 4.433 ± 0.572
0.739GluTrp: 0.739 ± 0.31
2.463GluTyr: 2.463 ± 0.52
0.0GluXaa: 0.0 ± 0.0
Phe
3.448PheAla: 3.448 ± 0.559
0.493PheCys: 0.493 ± 0.193
2.955PheAsp: 2.955 ± 0.467
2.955PheGlu: 2.955 ± 0.621
1.314PhePhe: 1.314 ± 0.371
2.545PheGly: 2.545 ± 0.5
0.739PheHis: 0.739 ± 0.239
1.806PheIle: 1.806 ± 0.404
1.97PheLys: 1.97 ± 0.408
2.627PheLeu: 2.627 ± 0.41
1.396PheMet: 1.396 ± 0.397
2.052PheAsn: 2.052 ± 0.376
2.299PhePro: 2.299 ± 0.431
1.806PheGln: 1.806 ± 0.472
2.299PheArg: 2.299 ± 0.435
1.806PheSer: 1.806 ± 0.346
2.299PheThr: 2.299 ± 0.553
3.612PheVal: 3.612 ± 0.575
0.575PheTrp: 0.575 ± 0.231
0.657PheTyr: 0.657 ± 0.168
0.0PheXaa: 0.0 ± 0.0
Gly
7.717GlyAla: 7.717 ± 0.942
0.821GlyCys: 0.821 ± 0.303
4.844GlyAsp: 4.844 ± 0.679
5.911GlyGlu: 5.911 ± 0.793
3.694GlyPhe: 3.694 ± 0.511
6.157GlyGly: 6.157 ± 0.969
1.067GlyHis: 1.067 ± 0.277
4.926GlyIle: 4.926 ± 0.655
5.911GlyLys: 5.911 ± 0.764
7.06GlyLeu: 7.06 ± 0.751
1.478GlyMet: 1.478 ± 0.402
2.627GlyAsn: 2.627 ± 0.395
2.873GlyPro: 2.873 ± 0.518
4.926GlyGln: 4.926 ± 0.67
4.105GlyArg: 4.105 ± 0.739
4.844GlySer: 4.844 ± 0.514
4.762GlyThr: 4.762 ± 0.719
5.582GlyVal: 5.582 ± 0.883
1.396GlyTrp: 1.396 ± 0.38
2.545GlyTyr: 2.545 ± 0.381
0.0GlyXaa: 0.0 ± 0.0
His
1.97HisAla: 1.97 ± 0.399
0.575HisCys: 0.575 ± 0.234
1.888HisAsp: 1.888 ± 0.498
0.493HisGlu: 0.493 ± 0.195
0.985HisPhe: 0.985 ± 0.346
1.56HisGly: 1.56 ± 0.371
0.821HisHis: 0.821 ± 0.334
1.478HisIle: 1.478 ± 0.377
1.149HisLys: 1.149 ± 0.253
1.642HisLeu: 1.642 ± 0.552
0.493HisMet: 0.493 ± 0.183
0.903HisAsn: 0.903 ± 0.314
0.657HisPro: 0.657 ± 0.253
0.575HisGln: 0.575 ± 0.216
1.149HisArg: 1.149 ± 0.313
0.575HisSer: 0.575 ± 0.174
1.231HisThr: 1.231 ± 0.358
1.149HisVal: 1.149 ± 0.352
0.657HisTrp: 0.657 ± 0.18
0.328HisTyr: 0.328 ± 0.17
0.0HisXaa: 0.0 ± 0.0
Ile
4.679IleAla: 4.679 ± 0.572
0.246IleCys: 0.246 ± 0.139
2.873IleAsp: 2.873 ± 0.579
3.694IleGlu: 3.694 ± 0.427
0.575IlePhe: 0.575 ± 0.264
4.433IleGly: 4.433 ± 0.701
1.149IleHis: 1.149 ± 0.333
2.955IleIle: 2.955 ± 0.515
2.381IleLys: 2.381 ± 0.514
3.858IleLeu: 3.858 ± 0.516
0.657IleMet: 0.657 ± 0.241
2.627IleAsn: 2.627 ± 0.459
2.134IlePro: 2.134 ± 0.482
2.627IleGln: 2.627 ± 0.474
2.545IleArg: 2.545 ± 0.42
1.97IleSer: 1.97 ± 0.315
4.433IleThr: 4.433 ± 0.618
3.776IleVal: 3.776 ± 0.517
0.493IleTrp: 0.493 ± 0.175
0.985IleTyr: 0.985 ± 0.243
0.0IleXaa: 0.0 ± 0.0
Lys
6.65LysAla: 6.65 ± 0.849
0.082LysCys: 0.082 ± 0.073
3.53LysAsp: 3.53 ± 0.445
3.448LysGlu: 3.448 ± 0.646
2.627LysPhe: 2.627 ± 0.42
4.351LysGly: 4.351 ± 0.709
0.985LysHis: 0.985 ± 0.336
2.627LysIle: 2.627 ± 0.369
2.545LysLys: 2.545 ± 0.428
4.269LysLeu: 4.269 ± 0.765
1.067LysMet: 1.067 ± 0.314
1.478LysAsn: 1.478 ± 0.341
2.627LysPro: 2.627 ± 0.52
1.642LysGln: 1.642 ± 0.348
3.858LysArg: 3.858 ± 0.651
2.627LysSer: 2.627 ± 0.313
3.12LysThr: 3.12 ± 0.664
4.351LysVal: 4.351 ± 0.788
0.821LysTrp: 0.821 ± 0.275
2.217LysTyr: 2.217 ± 0.428
0.0LysXaa: 0.0 ± 0.0
Leu
9.03LeuAla: 9.03 ± 1.095
0.328LeuCys: 0.328 ± 0.237
5.172LeuAsp: 5.172 ± 0.505
3.858LeuGlu: 3.858 ± 0.801
2.381LeuPhe: 2.381 ± 0.353
6.321LeuGly: 6.321 ± 0.965
1.724LeuHis: 1.724 ± 0.36
4.023LeuIle: 4.023 ± 0.54
5.829LeuLys: 5.829 ± 0.64
5.254LeuLeu: 5.254 ± 0.762
2.134LeuMet: 2.134 ± 0.373
2.709LeuAsn: 2.709 ± 0.404
3.858LeuPro: 3.858 ± 0.499
2.709LeuGln: 2.709 ± 0.385
5.5LeuArg: 5.5 ± 0.743
3.694LeuSer: 3.694 ± 0.539
4.105LeuThr: 4.105 ± 0.659
6.075LeuVal: 6.075 ± 0.693
1.56LeuTrp: 1.56 ± 0.313
2.545LeuTyr: 2.545 ± 0.531
0.0LeuXaa: 0.0 ± 0.0
Met
2.545MetAla: 2.545 ± 0.301
0.41MetCys: 0.41 ± 0.175
1.478MetAsp: 1.478 ± 0.377
1.314MetGlu: 1.314 ± 0.335
0.739MetPhe: 0.739 ± 0.213
1.642MetGly: 1.642 ± 0.372
0.164MetHis: 0.164 ± 0.108
0.985MetIle: 0.985 ± 0.267
0.657MetLys: 0.657 ± 0.25
1.231MetLeu: 1.231 ± 0.303
0.41MetMet: 0.41 ± 0.164
0.328MetAsn: 0.328 ± 0.127
0.903MetPro: 0.903 ± 0.253
0.903MetGln: 0.903 ± 0.224
0.903MetArg: 0.903 ± 0.231
1.97MetSer: 1.97 ± 0.597
1.888MetThr: 1.888 ± 0.412
1.478MetVal: 1.478 ± 0.297
0.0MetTrp: 0.0 ± 0.0
0.657MetTyr: 0.657 ± 0.241
0.0MetXaa: 0.0 ± 0.0
Asn
4.105AsnAla: 4.105 ± 0.477
0.164AsnCys: 0.164 ± 0.114
1.724AsnAsp: 1.724 ± 0.352
2.299AsnGlu: 2.299 ± 0.436
1.149AsnPhe: 1.149 ± 0.224
3.53AsnGly: 3.53 ± 0.651
0.821AsnHis: 0.821 ± 0.253
1.724AsnIle: 1.724 ± 0.395
2.381AsnLys: 2.381 ± 0.509
3.612AsnLeu: 3.612 ± 0.452
0.493AsnMet: 0.493 ± 0.209
1.314AsnAsn: 1.314 ± 0.429
2.463AsnPro: 2.463 ± 0.325
1.067AsnGln: 1.067 ± 0.265
2.052AsnArg: 2.052 ± 0.417
1.396AsnSer: 1.396 ± 0.28
1.97AsnThr: 1.97 ± 0.378
3.12AsnVal: 3.12 ± 0.612
0.493AsnTrp: 0.493 ± 0.215
1.396AsnTyr: 1.396 ± 0.262
0.0AsnXaa: 0.0 ± 0.0
Pro
4.762ProAla: 4.762 ± 0.789
0.739ProCys: 0.739 ± 0.24
2.381ProAsp: 2.381 ± 0.452
3.858ProGlu: 3.858 ± 0.487
2.134ProPhe: 2.134 ± 0.339
4.597ProGly: 4.597 ± 0.801
0.739ProHis: 0.739 ± 0.253
1.888ProIle: 1.888 ± 0.459
2.545ProLys: 2.545 ± 0.547
2.627ProLeu: 2.627 ± 0.439
0.821ProMet: 0.821 ± 0.259
1.97ProAsn: 1.97 ± 0.283
1.642ProPro: 1.642 ± 0.447
1.97ProGln: 1.97 ± 0.443
1.642ProArg: 1.642 ± 0.373
2.955ProSer: 2.955 ± 0.541
2.791ProThr: 2.791 ± 0.475
2.873ProVal: 2.873 ± 0.477
0.246ProTrp: 0.246 ± 0.163
1.314ProTyr: 1.314 ± 0.349
0.0ProXaa: 0.0 ± 0.0
Gln
5.582GlnAla: 5.582 ± 0.78
0.41GlnCys: 0.41 ± 0.206
2.299GlnAsp: 2.299 ± 0.342
3.366GlnGlu: 3.366 ± 0.459
2.217GlnPhe: 2.217 ± 0.38
2.791GlnGly: 2.791 ± 0.43
0.903GlnHis: 0.903 ± 0.262
2.299GlnIle: 2.299 ± 0.298
2.052GlnLys: 2.052 ± 0.372
3.12GlnLeu: 3.12 ± 0.46
0.739GlnMet: 0.739 ± 0.242
1.478GlnAsn: 1.478 ± 0.26
1.642GlnPro: 1.642 ± 0.238
2.709GlnGln: 2.709 ± 0.452
2.873GlnArg: 2.873 ± 0.425
2.791GlnSer: 2.791 ± 0.494
2.134GlnThr: 2.134 ± 0.347
2.791GlnVal: 2.791 ± 0.434
0.575GlnTrp: 0.575 ± 0.245
1.478GlnTyr: 1.478 ± 0.283
0.0GlnXaa: 0.0 ± 0.0
Arg
6.732ArgAla: 6.732 ± 0.566
0.739ArgCys: 0.739 ± 0.256
4.351ArgAsp: 4.351 ± 0.658
2.955ArgGlu: 2.955 ± 0.486
2.134ArgPhe: 2.134 ± 0.408
4.187ArgGly: 4.187 ± 0.607
1.478ArgHis: 1.478 ± 0.451
2.217ArgIle: 2.217 ± 0.502
3.038ArgLys: 3.038 ± 0.498
4.023ArgLeu: 4.023 ± 0.532
1.97ArgMet: 1.97 ± 0.368
2.627ArgAsn: 2.627 ± 0.427
2.299ArgPro: 2.299 ± 0.515
2.052ArgGln: 2.052 ± 0.497
3.448ArgArg: 3.448 ± 0.435
3.12ArgSer: 3.12 ± 0.43
3.612ArgThr: 3.612 ± 0.518
4.762ArgVal: 4.762 ± 0.504
1.231ArgTrp: 1.231 ± 0.351
1.231ArgTyr: 1.231 ± 0.284
0.0ArgXaa: 0.0 ± 0.0
Ser
5.09SerAla: 5.09 ± 0.811
0.41SerCys: 0.41 ± 0.202
3.12SerAsp: 3.12 ± 0.444
2.299SerGlu: 2.299 ± 0.432
2.299SerPhe: 2.299 ± 0.391
5.829SerGly: 5.829 ± 0.699
1.56SerHis: 1.56 ± 0.283
1.97SerIle: 1.97 ± 0.377
2.545SerLys: 2.545 ± 0.411
4.433SerLeu: 4.433 ± 0.513
0.575SerMet: 0.575 ± 0.21
1.642SerAsn: 1.642 ± 0.338
2.052SerPro: 2.052 ± 0.341
2.134SerGln: 2.134 ± 0.409
3.284SerArg: 3.284 ± 0.454
2.299SerSer: 2.299 ± 0.513
2.134SerThr: 2.134 ± 0.4
3.941SerVal: 3.941 ± 0.666
0.328SerTrp: 0.328 ± 0.19
1.56SerTyr: 1.56 ± 0.362
0.0SerXaa: 0.0 ± 0.0
Thr
6.157ThrAla: 6.157 ± 0.94
0.657ThrCys: 0.657 ± 0.281
3.941ThrAsp: 3.941 ± 0.63
2.955ThrGlu: 2.955 ± 0.5
3.941ThrPhe: 3.941 ± 0.752
5.582ThrGly: 5.582 ± 0.664
1.149ThrHis: 1.149 ± 0.44
3.038ThrIle: 3.038 ± 0.572
3.694ThrLys: 3.694 ± 0.626
4.762ThrLeu: 4.762 ± 0.48
1.314ThrMet: 1.314 ± 0.327
1.888ThrAsn: 1.888 ± 0.426
3.448ThrPro: 3.448 ± 0.46
2.463ThrGln: 2.463 ± 0.405
2.627ThrArg: 2.627 ± 0.442
2.299ThrSer: 2.299 ± 0.43
3.694ThrThr: 3.694 ± 0.74
5.09ThrVal: 5.09 ± 0.82
0.328ThrTrp: 0.328 ± 0.187
1.97ThrTyr: 1.97 ± 0.411
0.0ThrXaa: 0.0 ± 0.0
Val
7.963ValAla: 7.963 ± 0.58
0.493ValCys: 0.493 ± 0.195
4.433ValAsp: 4.433 ± 0.645
5.008ValGlu: 5.008 ± 0.561
1.806ValPhe: 1.806 ± 0.269
5.5ValGly: 5.5 ± 0.593
2.052ValHis: 2.052 ± 0.477
3.694ValIle: 3.694 ± 0.54
4.351ValLys: 4.351 ± 0.569
5.665ValLeu: 5.665 ± 0.641
1.396ValMet: 1.396 ± 0.266
3.12ValAsn: 3.12 ± 0.539
3.202ValPro: 3.202 ± 0.363
3.941ValGln: 3.941 ± 0.541
4.105ValArg: 4.105 ± 0.422
3.448ValSer: 3.448 ± 0.567
5.582ValThr: 5.582 ± 1.005
4.762ValVal: 4.762 ± 0.626
1.231ValTrp: 1.231 ± 0.244
1.149ValTyr: 1.149 ± 0.309
0.0ValXaa: 0.0 ± 0.0
Trp
1.231TrpAla: 1.231 ± 0.317
0.082TrpCys: 0.082 ± 0.087
0.739TrpAsp: 0.739 ± 0.24
0.739TrpGlu: 0.739 ± 0.247
0.739TrpPhe: 0.739 ± 0.241
1.396TrpGly: 1.396 ± 0.234
0.328TrpHis: 0.328 ± 0.154
0.985TrpIle: 0.985 ± 0.203
0.903TrpLys: 0.903 ± 0.275
1.314TrpLeu: 1.314 ± 0.345
0.246TrpMet: 0.246 ± 0.123
0.493TrpAsn: 0.493 ± 0.202
0.41TrpPro: 0.41 ± 0.149
0.41TrpGln: 0.41 ± 0.145
0.328TrpArg: 0.328 ± 0.13
1.067TrpSer: 1.067 ± 0.263
0.903TrpThr: 0.903 ± 0.309
1.314TrpVal: 1.314 ± 0.578
0.41TrpTrp: 0.41 ± 0.169
0.41TrpTyr: 0.41 ± 0.231
0.0TrpXaa: 0.0 ± 0.0
Tyr
3.12TyrAla: 3.12 ± 0.494
0.246TyrCys: 0.246 ± 0.128
1.396TyrAsp: 1.396 ± 0.371
1.642TyrGlu: 1.642 ± 0.36
0.657TyrPhe: 0.657 ± 0.195
2.299TyrGly: 2.299 ± 0.696
0.821TyrHis: 0.821 ± 0.268
1.478TyrIle: 1.478 ± 0.513
1.067TyrLys: 1.067 ± 0.236
2.627TyrLeu: 2.627 ± 0.609
0.575TyrMet: 0.575 ± 0.22
1.231TyrAsn: 1.231 ± 0.313
1.396TyrPro: 1.396 ± 0.379
1.478TyrGln: 1.478 ± 0.325
2.052TyrArg: 2.052 ± 0.513
1.314TyrSer: 1.314 ± 0.272
1.806TyrThr: 1.806 ± 0.385
1.806TyrVal: 1.806 ± 0.387
0.821TyrTrp: 0.821 ± 0.247
0.657TyrTyr: 0.657 ± 0.263
0.0TyrXaa: 0.0 ± 0.0
Xaa
0.0XaaAla: 0.0 ± 0.0
0.0XaaCys: 0.0 ± 0.0
0.0XaaAsp: 0.0 ± 0.0
0.0XaaGlu: 0.0 ± 0.0
0.0XaaPhe: 0.0 ± 0.0
0.0XaaGly: 0.0 ± 0.0
0.0XaaHis: 0.0 ± 0.0
0.0XaaIle: 0.0 ± 0.0
0.0XaaLys: 0.0 ± 0.0
0.0XaaLeu: 0.0 ± 0.0
0.0XaaMet: 0.0 ± 0.0
0.0XaaAsn: 0.0 ± 0.0
0.0XaaPro: 0.0 ± 0.0
0.0XaaGln: 0.0 ± 0.0
0.0XaaArg: 0.0 ± 0.0
0.0XaaSer: 0.0 ± 0.0
0.0XaaThr: 0.0 ± 0.0
0.0XaaVal: 0.0 ± 0.0
0.0XaaTrp: 0.0 ± 0.0
0.0XaaTyr: 0.0 ± 0.0
0.0XaaXaa: 0.0 ± 0.0
Statistics based on 52 proteins (12182 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski