Prevotella sp. CAG:255

Taxonomy: cellular organisms; Bacteria; FCB group; Bacteroidetes/Chlorobi group; Bacteroidetes; Bacteroidia; Bacteroidales; Prevotellaceae; Prevotella; environmental samples

Average proteome isoelectric point is 6.65

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 2771 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|R5CSU5|R5CSU5_9BACT Methylmalonyl-CoA decarboxylase OS=Prevotella sp. CAG:255 OX=1262923 GN=BN567_00833 PE=4 SV=1
MM1 pKa = 7.83EE2 pKa = 4.77KK3 pKa = 9.31TCIIYY8 pKa = 10.38GSSTGTCQAIAEE20 pKa = 4.48NIAGKK25 pKa = 10.28LGVGAADD32 pKa = 3.66VLDD35 pKa = 4.02VSSIDD40 pKa = 3.39ASTPDD45 pKa = 3.81AYY47 pKa = 10.05TNLILGTSTWGAGEE61 pKa = 4.8LQDD64 pKa = 4.33DD65 pKa = 4.49WYY67 pKa = 10.85DD68 pKa = 3.38GLEE71 pKa = 4.07ALKK74 pKa = 10.32QANLSGKK81 pKa = 8.43TIALFGCGDD90 pKa = 3.71SEE92 pKa = 5.35SYY94 pKa = 11.71GDD96 pKa = 4.09TFCSAIGEE104 pKa = 4.41IYY106 pKa = 10.5NALKK110 pKa = 10.76DD111 pKa = 3.69SGAKK115 pKa = 9.53FVGAVSTDD123 pKa = 2.75GYY125 pKa = 9.8TYY127 pKa = 10.78DD128 pKa = 4.59DD129 pKa = 3.56SAAVVDD135 pKa = 4.44GCFVGLALDD144 pKa = 5.18DD145 pKa = 4.05VNEE148 pKa = 4.32PDD150 pKa = 4.45KK151 pKa = 11.04TDD153 pKa = 3.37CRR155 pKa = 11.84IDD157 pKa = 3.01AWLEE161 pKa = 4.08SIKK164 pKa = 11.07NSLL167 pKa = 3.47

Molecular weight: 17.4 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|R5CV21|R5CV21_9BACT Putative N-carbamoylputrescine amidase OS=Prevotella sp. CAG:255 OX=1262923 GN=BN567_00175 PE=4 SV=1
MM1 pKa = 7.91PSGKK5 pKa = 9.62KK6 pKa = 10.03KK7 pKa = 10.01KK8 pKa = 7.32GHH10 pKa = 6.22KK11 pKa = 9.06MATHH15 pKa = 6.13KK16 pKa = 10.39RR17 pKa = 11.84KK18 pKa = 9.84KK19 pKa = 9.28RR20 pKa = 11.84LRR22 pKa = 11.84KK23 pKa = 9.25NRR25 pKa = 11.84HH26 pKa = 4.69KK27 pKa = 11.1SKK29 pKa = 11.1

Molecular weight: 3.51 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

2771

0

2771

951480

29

2842

343.4

38.57

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

7.445 ± 0.051

1.452 ± 0.02

5.97 ± 0.034

5.99 ± 0.043

4.317 ± 0.031

6.992 ± 0.044

1.792 ± 0.019

6.952 ± 0.05

6.626 ± 0.048

8.522 ± 0.048

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

3.074 ± 0.024

5.32 ± 0.039

3.463 ± 0.026

3.058 ± 0.023

4.697 ± 0.045

6.328 ± 0.042

5.743 ± 0.043

6.61 ± 0.037

1.185 ± 0.018

4.461 ± 0.033
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski