Amino acid dipepetide frequency for Prevotella sp. CAG:255

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10^-3.

For more information see sequence space article on Wikipedia.

	Ala	Cys	Asp	Glu	Phe	Gly	His	Ile	Lys	Leu	Met	Asn	Pro	Gln	Arg	Ser	Thr	Val	Trp	Tyr	Xaa
Ala	6.622AlaAla: 6.622 ± 0.129	1.102AlaCys: 1.102 ± 0.036	5.424AlaAsp: 5.424 ± 0.091	4.888AlaGlu: 4.888 ± 0.085	3.107AlaPhe: 3.107 ± 0.065	5.628AlaGly: 5.628 ± 0.097	1.175AlaHis: 1.175 ± 0.032	4.739AlaIle: 4.739 ± 0.074	4.414AlaLys: 4.414 ± 0.07	6.542AlaLeu: 6.542 ± 0.093	2.3AlaMet: 2.3 ± 0.058	3.133AlaAsn: 3.133 ± 0.058	2.152AlaPro: 2.152 ± 0.05	2.513AlaGln: 2.513 ± 0.047	3.271AlaArg: 3.271 ± 0.074	4.285AlaSer: 4.285 ± 0.072	4.031AlaThr: 4.031 ± 0.067	5.342AlaVal: 5.342 ± 0.091	0.72AlaTrp: 0.72 ± 0.028	3.06AlaTyr: 3.06 ± 0.062	0.001AlaXaa: 0.001 ± 0.001
Cys	0.994CysAla: 0.994 ± 0.037	0.271CysCys: 0.271 ± 0.021	0.817CysAsp: 0.817 ± 0.03	0.724CysGlu: 0.724 ± 0.03	0.673CysPhe: 0.673 ± 0.032	1.287CysGly: 1.287 ± 0.042	0.327CysHis: 0.327 ± 0.019	0.997CysIle: 0.997 ± 0.032	0.818CysLys: 0.818 ± 0.033	1.202CysLeu: 1.202 ± 0.035	0.409CysMet: 0.409 ± 0.022	0.704CysAsn: 0.704 ± 0.025	0.611CysPro: 0.611 ± 0.026	0.334CysGln: 0.334 ± 0.019	0.808CysArg: 0.808 ± 0.035	0.987CysSer: 0.987 ± 0.039	0.751CysThr: 0.751 ± 0.03	0.977CysVal: 0.977 ± 0.034	0.18CysTrp: 0.18 ± 0.013	0.644CysTyr: 0.644 ± 0.027	0.0CysXaa: 0.0 ± 0.0
Asp	4.293AspAla: 4.293 ± 0.066	0.763AspCys: 0.763 ± 0.029	3.476AspAsp: 3.476 ± 0.062	4.186AspGlu: 4.186 ± 0.077	2.973AspPhe: 2.973 ± 0.05	5.138AspGly: 5.138 ± 0.1	0.795AspHis: 0.795 ± 0.033	4.577AspIle: 4.577 ± 0.08	4.266AspLys: 4.266 ± 0.075	4.161AspLeu: 4.161 ± 0.081	1.969AspMet: 1.969 ± 0.05	3.562AspAsn: 3.562 ± 0.063	1.773AspPro: 1.773 ± 0.05	1.116AspGln: 1.116 ± 0.038	2.451AspArg: 2.451 ± 0.055	3.476AspSer: 3.476 ± 0.064	2.968AspThr: 2.968 ± 0.058	4.082AspVal: 4.082 ± 0.075	0.771AspTrp: 0.771 ± 0.029	2.902AspTyr: 2.902 ± 0.063	0.0AspXaa: 0.0 ± 0.0
Glu	4.669GluAla: 4.669 ± 0.083	0.734GluCys: 0.734 ± 0.029	3.115GluAsp: 3.115 ± 0.06	4.119GluGlu: 4.119 ± 0.09	2.285GluPhe: 2.285 ± 0.049	3.975GluGly: 3.975 ± 0.069	1.194GluHis: 1.194 ± 0.034	4.18GluIle: 4.18 ± 0.075	4.509GluLys: 4.509 ± 0.08	5.26GluLeu: 5.26 ± 0.087	1.936GluMet: 1.936 ± 0.05	3.438GluAsn: 3.438 ± 0.062	1.701GluPro: 1.701 ± 0.042	2.328GluGln: 2.328 ± 0.05	3.084GluArg: 3.084 ± 0.053	3.057GluSer: 3.057 ± 0.064	3.23GluThr: 3.23 ± 0.068	3.503GluVal: 3.503 ± 0.07	0.83GluTrp: 0.83 ± 0.031	2.754GluTyr: 2.754 ± 0.061	0.0GluXaa: 0.0 ± 0.0
Phe	2.901PheAla: 2.901 ± 0.063	0.808PheCys: 0.808 ± 0.028	2.717PheAsp: 2.717 ± 0.047	2.192PheGlu: 2.192 ± 0.047	1.954PhePhe: 1.954 ± 0.055	3.247PheGly: 3.247 ± 0.068	0.758PheHis: 0.758 ± 0.03	3.132PheIle: 3.132 ± 0.069	2.492PheLys: 2.492 ± 0.052	3.264PheLeu: 3.264 ± 0.065	1.404PheMet: 1.404 ± 0.041	2.449PheAsn: 2.449 ± 0.055	1.348PhePro: 1.348 ± 0.036	0.923PheGln: 0.923 ± 0.033	2.021PheArg: 2.021 ± 0.043	3.443PheSer: 3.443 ± 0.062	2.703PheThr: 2.703 ± 0.057	3.015PheVal: 3.015 ± 0.063	0.481PheTrp: 0.481 ± 0.025	1.915PheTyr: 1.915 ± 0.047	0.001PheXaa: 0.001 ± 0.001
Gly	4.642GlyAla: 4.642 ± 0.086	1.13GlyCys: 1.13 ± 0.046	3.747GlyAsp: 3.747 ± 0.074	3.985GlyGlu: 3.985 ± 0.068	3.046GlyPhe: 3.046 ± 0.058	4.956GlyGly: 4.956 ± 0.094	1.399GlyHis: 1.399 ± 0.04	5.335GlyIle: 5.335 ± 0.069	5.069GlyLys: 5.069 ± 0.08	5.732GlyLeu: 5.732 ± 0.084	2.29GlyMet: 2.29 ± 0.055	3.812GlyAsn: 3.812 ± 0.081	1.273GlyPro: 1.273 ± 0.039	2.146GlyGln: 2.146 ± 0.04	3.46GlyArg: 3.46 ± 0.064	4.424GlySer: 4.424 ± 0.084	4.348GlyThr: 4.348 ± 0.084	4.855GlyVal: 4.855 ± 0.078	0.943GlyTrp: 0.943 ± 0.035	3.331GlyTyr: 3.331 ± 0.069	0.001GlyXaa: 0.001 ± 0.001
His	1.18HisAla: 1.18 ± 0.04	0.331HisCys: 0.331 ± 0.02	1.089HisAsp: 1.089 ± 0.031	0.993HisGlu: 0.993 ± 0.035	0.895HisPhe: 0.895 ± 0.03	1.308HisGly: 1.308 ± 0.042	0.46HisHis: 0.46 ± 0.025	1.348HisIle: 1.348 ± 0.038	1.167HisLys: 1.167 ± 0.037	1.39HisLeu: 1.39 ± 0.035	0.395HisMet: 0.395 ± 0.02	1.003HisAsn: 1.003 ± 0.035	0.867HisPro: 0.867 ± 0.033	0.479HisGln: 0.479 ± 0.023	0.842HisArg: 0.842 ± 0.033	1.031HisSer: 1.031 ± 0.031	0.973HisThr: 0.973 ± 0.026	1.12HisVal: 1.12 ± 0.039	0.203HisTrp: 0.203 ± 0.014	0.846HisTyr: 0.846 ± 0.032	0.0HisXaa: 0.0 ± 0.0
Ile	5.008IleAla: 5.008 ± 0.089	1.172IleCys: 1.172 ± 0.038	4.617IleAsp: 4.617 ± 0.072	4.278IleGlu: 4.278 ± 0.069	2.625IlePhe: 2.625 ± 0.054	4.589IleGly: 4.589 ± 0.077	1.131IleHis: 1.131 ± 0.037	5.057IleIle: 5.057 ± 0.087	4.782IleLys: 4.782 ± 0.073	5.251IleLeu: 5.251 ± 0.096	1.889IleMet: 1.889 ± 0.054	3.954IleAsn: 3.954 ± 0.072	2.718IlePro: 2.718 ± 0.054	1.731IleGln: 1.731 ± 0.045	3.206IleArg: 3.206 ± 0.061	5.048IleSer: 5.048 ± 0.069	4.355IleThr: 4.355 ± 0.073	4.677IleVal: 4.677 ± 0.077	0.62IleTrp: 0.62 ± 0.025	2.817IleTyr: 2.817 ± 0.052	0.0IleXaa: 0.0 ± 0.0
Lys	5.292LysAla: 5.292 ± 0.078	0.655LysCys: 0.655 ± 0.026	4.155LysAsp: 4.155 ± 0.068	4.7LysGlu: 4.7 ± 0.09	2.364LysPhe: 2.364 ± 0.049	4.243LysGly: 4.243 ± 0.072	1.093LysHis: 1.093 ± 0.035	4.459LysIle: 4.459 ± 0.068	4.868LysLys: 4.868 ± 0.086	5.317LysLeu: 5.317 ± 0.074	2.282LysMet: 2.282 ± 0.048	3.778LysAsn: 3.778 ± 0.071	2.241LysPro: 2.241 ± 0.052	2.205LysGln: 2.205 ± 0.052	3.079LysArg: 3.079 ± 0.069	3.623LysSer: 3.623 ± 0.066	3.82LysThr: 3.82 ± 0.065	4.166LysVal: 4.166 ± 0.07	0.768LysTrp: 0.768 ± 0.031	3.156LysTyr: 3.156 ± 0.063	0.0LysXaa: 0.0 ± 0.0
Leu	6.139LeuAla: 6.139 ± 0.091	1.462LeuCys: 1.462 ± 0.04	4.505LeuAsp: 4.505 ± 0.078	4.094LeuGlu: 4.094 ± 0.074	3.855LeuPhe: 3.855 ± 0.065	5.418LeuGly: 5.418 ± 0.089	1.58LeuHis: 1.58 ± 0.037	5.329LeuIle: 5.329 ± 0.086	5.927LeuLys: 5.927 ± 0.084	7.731LeuLeu: 7.731 ± 0.096	2.672LeuMet: 2.672 ± 0.058	4.592LeuAsn: 4.592 ± 0.069	3.61LeuPro: 3.61 ± 0.061	2.739LeuGln: 2.739 ± 0.059	4.257LeuArg: 4.257 ± 0.075	6.497LeuSer: 6.497 ± 0.089	5.437LeuThr: 5.437 ± 0.079	4.842LeuVal: 4.842 ± 0.079	0.955LeuTrp: 0.955 ± 0.036	3.579LeuTyr: 3.579 ± 0.064	0.0LeuXaa: 0.0 ± 0.0
Met	2.713MetAla: 2.713 ± 0.054	0.378MetCys: 0.378 ± 0.023	1.402MetAsp: 1.402 ± 0.036	1.621MetGlu: 1.621 ± 0.047	1.228MetPhe: 1.228 ± 0.039	1.836MetGly: 1.836 ± 0.044	0.59MetHis: 0.59 ± 0.021	1.768MetIle: 1.768 ± 0.042	2.62MetLys: 2.62 ± 0.058	3.053MetLeu: 3.053 ± 0.059	1.003MetMet: 1.003 ± 0.032	1.633MetAsn: 1.633 ± 0.046	1.465MetPro: 1.465 ± 0.041	1.159MetGln: 1.159 ± 0.035	1.624MetArg: 1.624 ± 0.043	2.025MetSer: 2.025 ± 0.047	1.732MetThr: 1.732 ± 0.039	1.654MetVal: 1.654 ± 0.048	0.266MetTrp: 0.266 ± 0.016	0.973MetTyr: 0.973 ± 0.032	0.0MetXaa: 0.0 ± 0.0
Asn	3.78AsnAla: 3.78 ± 0.062	0.675AsnCys: 0.675 ± 0.029	3.154AsnAsp: 3.154 ± 0.061	3.025AsnGlu: 3.025 ± 0.055	2.204AsnPhe: 2.204 ± 0.052	4.339AsnGly: 4.339 ± 0.084	0.815AsnHis: 0.815 ± 0.032	4.387AsnIle: 4.387 ± 0.073	3.317AsnLys: 3.317 ± 0.069	4.105AsnLeu: 4.105 ± 0.074	1.512AsnMet: 1.512 ± 0.039	3.097AsnAsn: 3.097 ± 0.083	2.244AsnPro: 2.244 ± 0.057	1.323AsnGln: 1.323 ± 0.04	2.386AsnArg: 2.386 ± 0.056	3.203AsnSer: 3.203 ± 0.061	3.065AsnThr: 3.065 ± 0.067	3.643AsnVal: 3.643 ± 0.066	0.6AsnTrp: 0.6 ± 0.026	2.324AsnTyr: 2.324 ± 0.047	0.001AsnXaa: 0.001 ± 0.001
Pro	2.676ProAla: 2.676 ± 0.057	0.43ProCys: 0.43 ± 0.021	2.595ProAsp: 2.595 ± 0.053	3.064ProGlu: 3.064 ± 0.058	1.613ProPhe: 1.613 ± 0.052	2.304ProGly: 2.304 ± 0.051	0.657ProHis: 0.657 ± 0.029	1.951ProIle: 1.951 ± 0.047	1.86ProLys: 1.86 ± 0.047	2.919ProLeu: 2.919 ± 0.058	0.987ProMet: 0.987 ± 0.029	1.433ProAsn: 1.433 ± 0.042	0.685ProPro: 0.685 ± 0.03	1.296ProGln: 1.296 ± 0.035	1.257ProArg: 1.257 ± 0.037	2.117ProSer: 2.117 ± 0.048	1.784ProThr: 1.784 ± 0.041	2.888ProVal: 2.888 ± 0.055	0.438ProTrp: 0.438 ± 0.021	1.674ProTyr: 1.674 ± 0.042	0.001ProXaa: 0.001 ± 0.001
Gln	2.299GlnAla: 2.299 ± 0.045	0.371GlnCys: 0.371 ± 0.021	1.457GlnAsp: 1.457 ± 0.044	1.662GlnGlu: 1.662 ± 0.041	1.166GlnPhe: 1.166 ± 0.032	1.798GlnGly: 1.798 ± 0.043	0.581GlnHis: 0.581 ± 0.026	2.255GlnIle: 2.255 ± 0.051	2.13GlnLys: 2.13 ± 0.053	2.974GlnLeu: 2.974 ± 0.063	1.068GlnMet: 1.068 ± 0.033	1.701GlnAsn: 1.701 ± 0.047	1.163GlnPro: 1.163 ± 0.035	1.324GlnGln: 1.324 ± 0.041	1.591GlnArg: 1.591 ± 0.042	1.613GlnSer: 1.613 ± 0.038	1.955GlnThr: 1.955 ± 0.045	1.664GlnVal: 1.664 ± 0.046	0.447GlnTrp: 0.447 ± 0.025	1.364GlnTyr: 1.364 ± 0.042	0.001GlnXaa: 0.001 ± 0.001
Arg	2.869ArgAla: 2.869 ± 0.063	0.63ArgCys: 0.63 ± 0.03	2.273ArgAsp: 2.273 ± 0.045	2.679ArgGlu: 2.679 ± 0.063	2.126ArgPhe: 2.126 ± 0.046	2.602ArgGly: 2.602 ± 0.058	1.154ArgHis: 1.154 ± 0.039	3.559ArgIle: 3.559 ± 0.061	3.365ArgLys: 3.365 ± 0.068	4.651ArgLeu: 4.651 ± 0.079	1.704ArgMet: 1.704 ± 0.041	2.632ArgAsn: 2.632 ± 0.055	1.673ArgPro: 1.673 ± 0.043	2.02ArgGln: 2.02 ± 0.045	2.735ArgArg: 2.735 ± 0.073	2.465ArgSer: 2.465 ± 0.056	2.454ArgThr: 2.454 ± 0.056	2.574ArgVal: 2.574 ± 0.052	0.617ArgTrp: 0.617 ± 0.025	2.19ArgTyr: 2.19 ± 0.051	0.001ArgXaa: 0.001 ± 0.001
Ser	4.721SerAla: 4.721 ± 0.08	0.978SerCys: 0.978 ± 0.037	3.845SerAsp: 3.845 ± 0.074	3.561SerGlu: 3.561 ± 0.068	2.947SerPhe: 2.947 ± 0.052	4.799SerGly: 4.799 ± 0.084	1.193SerHis: 1.193 ± 0.037	4.234SerIle: 4.234 ± 0.085	3.54SerLys: 3.54 ± 0.069	6.077SerLeu: 6.077 ± 0.096	1.791SerMet: 1.791 ± 0.047	2.967SerAsn: 2.967 ± 0.054	2.153SerPro: 2.153 ± 0.044	1.915SerGln: 1.915 ± 0.044	2.927SerArg: 2.927 ± 0.061	4.036SerSer: 4.036 ± 0.081	3.25SerThr: 3.25 ± 0.06	4.648SerVal: 4.648 ± 0.087	0.794SerTrp: 0.794 ± 0.032	2.904SerTyr: 2.904 ± 0.062	0.0SerXaa: 0.0 ± 0.0
Thr	4.775ThrAla: 4.775 ± 0.074	0.722ThrCys: 0.722 ± 0.027	3.927ThrAsp: 3.927 ± 0.072	3.236ThrGlu: 3.236 ± 0.061	2.707ThrPhe: 2.707 ± 0.058	4.444ThrGly: 4.444 ± 0.076	0.966ThrHis: 0.966 ± 0.03	3.959ThrIle: 3.959 ± 0.074	2.952ThrLys: 2.952 ± 0.055	5.439ThrLeu: 5.439 ± 0.084	1.451ThrMet: 1.451 ± 0.039	2.368ThrAsn: 2.368 ± 0.053	2.412ThrPro: 2.412 ± 0.048	1.583ThrGln: 1.583 ± 0.042	2.205ThrArg: 2.205 ± 0.05	3.505ThrSer: 3.505 ± 0.074	3.359ThrThr: 3.359 ± 0.07	4.14ThrVal: 4.14 ± 0.073	0.712ThrTrp: 0.712 ± 0.028	2.572ThrTyr: 2.572 ± 0.058	0.0ThrXaa: 0.0 ± 0.0
Val	5.0ValAla: 5.0 ± 0.095	1.1ValCys: 1.1 ± 0.036	3.853ValAsp: 3.853 ± 0.069	3.884ValGlu: 3.884 ± 0.065	2.866ValPhe: 2.866 ± 0.062	3.974ValGly: 3.974 ± 0.086	0.984ValHis: 0.984 ± 0.035	4.47ValIle: 4.47 ± 0.082	4.549ValLys: 4.549 ± 0.078	5.365ValLeu: 5.365 ± 0.072	2.006ValMet: 2.006 ± 0.048	3.483ValAsn: 3.483 ± 0.07	2.571ValPro: 2.571 ± 0.062	1.696ValGln: 1.696 ± 0.044	3.079ValArg: 3.079 ± 0.065	4.904ValSer: 4.904 ± 0.096	3.848ValThr: 3.848 ± 0.07	4.83ValVal: 4.83 ± 0.089	0.801ValTrp: 0.801 ± 0.032	2.832ValTyr: 2.832 ± 0.052	0.002ValXaa: 0.002 ± 0.001
Trp	0.759TrpAla: 0.759 ± 0.033	0.178TrpCys: 0.178 ± 0.015	0.637TrpAsp: 0.637 ± 0.029	0.582TrpGlu: 0.582 ± 0.024	0.514TrpPhe: 0.514 ± 0.024	0.863TrpGly: 0.863 ± 0.032	0.301TrpHis: 0.301 ± 0.019	0.823TrpIle: 0.823 ± 0.031	0.772TrpLys: 0.772 ± 0.029	1.209TrpLeu: 1.209 ± 0.04	0.423TrpMet: 0.423 ± 0.02	0.758TrpAsn: 0.758 ± 0.03	0.302TrpPro: 0.302 ± 0.018	0.478TrpGln: 0.478 ± 0.026	0.619TrpArg: 0.619 ± 0.027	0.684TrpSer: 0.684 ± 0.031	0.699TrpThr: 0.699 ± 0.03	0.562TrpVal: 0.562 ± 0.022	0.177TrpTrp: 0.177 ± 0.014	0.513TrpTyr: 0.513 ± 0.024	0.0TrpXaa: 0.0 ± 0.0
Tyr	3.119TyrAla: 3.119 ± 0.063	0.625TyrCys: 0.625 ± 0.025	2.894TyrAsp: 2.894 ± 0.06	2.426TyrGlu: 2.426 ± 0.053	2.021TyrPhe: 2.021 ± 0.051	3.175TyrGly: 3.175 ± 0.06	0.772TyrHis: 0.772 ± 0.029	2.963TyrIle: 2.963 ± 0.06	2.847TyrLys: 2.847 ± 0.055	3.571TyrLeu: 3.571 ± 0.07	1.253TyrMet: 1.253 ± 0.038	2.703TyrAsn: 2.703 ± 0.065	1.663TyrPro: 1.663 ± 0.043	1.274TyrGln: 1.274 ± 0.038	2.073TyrArg: 2.073 ± 0.05	2.855TyrSer: 2.855 ± 0.062	2.672TyrThr: 2.672 ± 0.067	2.915TyrVal: 2.915 ± 0.058	0.529TyrTrp: 0.529 ± 0.023	2.26TyrTyr: 2.26 ± 0.059	0.0TyrXaa: 0.0 ± 0.0
Xaa	0.0XaaAla: 0.0 ± 0.0	0.001XaaCys: 0.001 ± 0.001	0.001XaaAsp: 0.001 ± 0.001	0.0XaaGlu: 0.0 ± 0.0	0.0XaaPhe: 0.0 ± 0.0	0.0XaaGly: 0.0 ± 0.0	0.0XaaHis: 0.0 ± 0.0	0.001XaaIle: 0.001 ± 0.001	0.0XaaLys: 0.0 ± 0.0	0.002XaaLeu: 0.002 ± 0.001	0.0XaaMet: 0.0 ± 0.0	0.0XaaAsn: 0.0 ± 0.0	0.002XaaPro: 0.002 ± 0.002	0.001XaaGln: 0.001 ± 0.001	0.0XaaArg: 0.0 ± 0.0	0.001XaaSer: 0.001 ± 0.001	0.0XaaThr: 0.0 ± 0.0	0.0XaaVal: 0.0 ± 0.0	0.0XaaTrp: 0.0 ± 0.0	0.0XaaTyr: 0.0 ± 0.0	0.016XaaXaa: 0.016 ± 0.006

Statistics based on 2771 proteins (951480 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944

Contact: Lukasz P. Kozlowski

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