Sodalis sp. TME1
Average proteome isoelectric point is 6.79
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 2289 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A1J7D2U5|A0A1J7D2U5_9GAMM Outer membrane protein A OS=Sodalis sp. TME1 OX=1912097 GN=ompA PE=3 SV=1
MM1 pKa = 7.59 SEE3 pKa = 3.97 QNNTEE8 pKa = 3.83 MAFQIQRR15 pKa = 11.84 IYY17 pKa = 10.13 TKK19 pKa = 10.3 DD20 pKa = 3.43 VSFEE24 pKa = 4.02 APNAPKK30 pKa = 10.45 VFQQEE35 pKa = 3.95 WQPEE39 pKa = 4.07 IKK41 pKa = 10.36 LDD43 pKa = 3.84 LDD45 pKa = 3.92 TASSQLAEE53 pKa = 4.5 EE54 pKa = 4.57 IYY56 pKa = 10.6 EE57 pKa = 4.17 VVLRR61 pKa = 11.84 VTVTATLGDD70 pKa = 3.8 DD71 pKa = 3.5 TAFLCEE77 pKa = 4.07 VQQAGIFTISGIDD90 pKa = 3.6 GTQMAHH96 pKa = 7.07 CLGAYY101 pKa = 9.15 CPNILFPYY109 pKa = 9.96 ARR111 pKa = 11.84 EE112 pKa = 4.5 CITSQVSRR120 pKa = 11.84 GTFPQLNLAPVNFDD134 pKa = 3.64 ALFMNYY140 pKa = 9.34 LQQQADD146 pKa = 4.24 GEE148 pKa = 4.82 GSQPAQDD155 pKa = 3.29 AA156 pKa = 3.8
Molecular weight: 17.31 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.819
IPC2_protein 4.062
IPC_protein 3.986
Toseland 3.795
ProMoST 4.113
Dawson 3.948
Bjellqvist 4.101
Wikipedia 3.846
Rodwell 3.821
Grimsley 3.706
Solomon 3.935
Lehninger 3.897
Nozaki 4.062
DTASelect 4.228
Thurlkill 3.834
EMBOSS 3.859
Sillero 4.101
Patrickios 0.947
IPC_peptide 3.935
IPC2_peptide 4.075
IPC2.peptide.svr19 3.978
Protein with the highest isoelectric point:
>tr|A0A1J7D1T7|A0A1J7D1T7_9GAMM Tyr recombinase domain-containing protein OS=Sodalis sp. TME1 OX=1912097 GN=BK025_17425 PE=4 SV=1
MM1 pKa = 7.01 SCWWKK6 pKa = 10.02 PLSQLCLLLFPLTVAAEE23 pKa = 4.15 NGDD26 pKa = 3.87 ALVTRR31 pKa = 11.84 LEE33 pKa = 4.1 SLLRR37 pKa = 11.84 QQRR40 pKa = 11.84 VDD42 pKa = 2.99 SSLVQKK48 pKa = 9.29 VTLKK52 pKa = 10.16 TPAKK56 pKa = 9.52 LLPGCPSPHH65 pKa = 6.43 LQLASHH71 pKa = 6.47 GRR73 pKa = 11.84 LSGNMSVTARR83 pKa = 11.84 CGDD86 pKa = 3.01 RR87 pKa = 11.84 RR88 pKa = 11.84 YY89 pKa = 10.03 FLQIQVRR96 pKa = 11.84 ARR98 pKa = 11.84 GDD100 pKa = 3.09 YY101 pKa = 8.89 WVAARR106 pKa = 11.84 ALYY109 pKa = 10.69 AGTSLSAKK117 pKa = 10.27 DD118 pKa = 3.12 IVKK121 pKa = 10.09 EE122 pKa = 3.8 HH123 pKa = 6.82 GEE125 pKa = 4.04 LVGQARR131 pKa = 11.84 NVIFATQPVTGSLTTRR147 pKa = 11.84 TIQAGQPLAATMLRR161 pKa = 11.84 APWKK165 pKa = 9.52 VRR167 pKa = 11.84 QGNPVAVVSRR177 pKa = 11.84 GPGFQVRR184 pKa = 11.84 HH185 pKa = 5.45 YY186 pKa = 9.63 GKK188 pKa = 10.6 AMSDD192 pKa = 3.24 AAVGDD197 pKa = 3.92 TLRR200 pKa = 11.84 VRR202 pKa = 11.84 LASGQMVSGAVDD214 pKa = 3.28 NQGVVLQNEE223 pKa = 4.43 RR224 pKa = 11.84 AFSSFSAPGRR234 pKa = 3.74
Molecular weight: 25.28 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.354
IPC2_protein 9.648
IPC_protein 10.54
Toseland 10.73
ProMoST 10.687
Dawson 10.818
Bjellqvist 10.555
Wikipedia 11.052
Rodwell 10.979
Grimsley 10.862
Solomon 10.965
Lehninger 10.935
Nozaki 10.73
DTASelect 10.555
Thurlkill 10.73
EMBOSS 11.14
Sillero 10.76
Patrickios 10.701
IPC_peptide 10.965
IPC2_peptide 9.75
IPC2.peptide.svr19 8.683
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
2289
0
2289
562918
29
1342
245.9
27.19
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
10.379 ± 0.075
1.112 ± 0.021
5.408 ± 0.042
5.419 ± 0.056
3.641 ± 0.033
7.394 ± 0.051
2.367 ± 0.025
5.63 ± 0.044
3.911 ± 0.044
11.033 ± 0.074
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.701 ± 0.022
3.412 ± 0.042
4.599 ± 0.035
4.617 ± 0.043
6.535 ± 0.053
5.576 ± 0.039
5.201 ± 0.03
6.931 ± 0.047
1.342 ± 0.023
2.785 ± 0.032
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here