Mesorhizobium sp. YR577
Average proteome isoelectric point is 6.51
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 6444 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A1I7D715|A0A1I7D715_9RHIZ Crotonobetainyl-CoA hydratase OS=Mesorhizobium sp. YR577 OX=1884373 GN=SAMN05518861_111218 PE=3 SV=1
MM1 pKa = 6.86 NTVLNAKK8 pKa = 8.71 GVPLPYY14 pKa = 10.09 SGSSVNHH21 pKa = 6.18 FSATNSGPQLAGSALNDD38 pKa = 3.96 SMWGDD43 pKa = 3.41 SSVNVVMQGGTGDD56 pKa = 4.42 DD57 pKa = 2.84 IYY59 pKa = 11.62 YY60 pKa = 10.35 LYY62 pKa = 10.59 SARR65 pKa = 11.84 NSAFEE70 pKa = 3.96 KK71 pKa = 10.56 AGEE74 pKa = 4.29 GVDD77 pKa = 5.09 TINTWMSYY85 pKa = 10.37 RR86 pKa = 11.84 LPEE89 pKa = 4.5 NIEE92 pKa = 3.93 NLTVTGNGRR101 pKa = 11.84 SAIGNDD107 pKa = 3.26 GDD109 pKa = 4.73 NILSGASGSQTIDD122 pKa = 2.68 GGAGDD127 pKa = 4.95 DD128 pKa = 3.76 VLIGGGGADD137 pKa = 2.8 IFVISEE143 pKa = 4.3 GNGSDD148 pKa = 4.84 LILDD152 pKa = 4.16 FSVDD156 pKa = 3.47 DD157 pKa = 3.73 QVRR160 pKa = 11.84 LDD162 pKa = 3.67 GYY164 pKa = 11.11 GFISFDD170 pKa = 4.09 AIQSNMTQTGANVTLDD186 pKa = 3.79 LGNDD190 pKa = 3.81 EE191 pKa = 4.85 ILVFANTTVDD201 pKa = 3.77 QFDD204 pKa = 3.49 AGQFKK209 pKa = 11.03 LSLDD213 pKa = 3.54 KK214 pKa = 11.58 SEE216 pKa = 4.57 MSLSFSDD223 pKa = 4.84 DD224 pKa = 3.76 FNSLSLWNGEE234 pKa = 4.05 SGTWDD239 pKa = 3.43 SNFWWGAEE247 pKa = 3.87 NGSTHH252 pKa = 6.55 EE253 pKa = 4.64 GNGEE257 pKa = 4.01 KK258 pKa = 9.65 QWYY261 pKa = 9.12 IDD263 pKa = 3.29 TDD265 pKa = 3.88 YY266 pKa = 11.83 APTKK270 pKa = 10.1 SVNPFSVDD278 pKa = 3.1 NGVLTITAAPTPDD291 pKa = 4.15 AIKK294 pKa = 10.72 PEE296 pKa = 4.19 VNNYY300 pKa = 10.76 DD301 pKa = 3.71 YY302 pKa = 11.4 TSGLLTTYY310 pKa = 10.65 EE311 pKa = 4.7 SFSQTYY317 pKa = 10.09 GYY319 pKa = 11.18 FEE321 pKa = 4.44 MRR323 pKa = 11.84 ADD325 pKa = 3.44 MPEE328 pKa = 3.97 NQGVWPAFWLLPADD342 pKa = 4.39 GSWPPEE348 pKa = 3.76 LDD350 pKa = 3.52 VVEE353 pKa = 4.68 MRR355 pKa = 11.84 GQEE358 pKa = 3.94 PGVVHH363 pKa = 5.48 VTSHH367 pKa = 5.1 TNEE370 pKa = 4.46 TGSHH374 pKa = 5.33 TSVSSAVNVPDD385 pKa = 3.19 TSGFHH390 pKa = 6.35 TYY392 pKa = 9.8 GVLWTEE398 pKa = 4.54 EE399 pKa = 4.13 EE400 pKa = 4.36 IVWYY404 pKa = 10.3 FDD406 pKa = 3.31 DD407 pKa = 4.97 VEE409 pKa = 4.25 IARR412 pKa = 11.84 TDD414 pKa = 3.66 TPSDD418 pKa = 3.26 MHH420 pKa = 8.21 GPMYY424 pKa = 10.15 MLVNLAVGGVAGTPADD440 pKa = 3.9 GLATPAEE447 pKa = 4.02 MQIDD451 pKa = 4.18 YY452 pKa = 10.81 IKK454 pKa = 10.54 AYY456 pKa = 9.55 EE457 pKa = 4.09 LDD459 pKa = 3.71 GVTQAAAKK467 pKa = 10.15 AGSGDD472 pKa = 3.65 FLVV475 pKa = 4.16
Molecular weight: 50.81 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.682
IPC2_protein 3.732
IPC_protein 3.77
Toseland 3.541
ProMoST 3.923
Dawson 3.757
Bjellqvist 3.91
Wikipedia 3.694
Rodwell 3.592
Grimsley 3.439
Solomon 3.757
Lehninger 3.706
Nozaki 3.872
DTASelect 4.126
Thurlkill 3.592
EMBOSS 3.706
Sillero 3.884
Patrickios 1.341
IPC_peptide 3.745
IPC2_peptide 3.859
IPC2.peptide.svr19 3.786
Protein with the highest isoelectric point:
>tr|A0A1I6ZVZ7|A0A1I6ZVZ7_9RHIZ Manganese/iron transport system permease protein OS=Mesorhizobium sp. YR577 OX=1884373 GN=SAMN05518861_103255 PE=3 SV=1
MM1 pKa = 8.09 DD2 pKa = 4.95 KK3 pKa = 11.06 GDD5 pKa = 3.98 RR6 pKa = 11.84 RR7 pKa = 11.84 PLASRR12 pKa = 11.84 NTRR15 pKa = 11.84 WAQTIAKK22 pKa = 9.45 RR23 pKa = 11.84 MAALSITPNAISQASIAAAAVAGLAFWIAGEE54 pKa = 4.17 TSGTARR60 pKa = 11.84 SLLLVAAALFCQLRR74 pKa = 11.84 LLCNLFDD81 pKa = 3.98 GMVAVEE87 pKa = 4.26 GGKK90 pKa = 9.78 AAADD94 pKa = 3.84 GPFWNEE100 pKa = 3.4 FPDD103 pKa = 4.88 RR104 pKa = 11.84 IADD107 pKa = 3.62 ILILAGVGYY116 pKa = 10.69 GIGAPSLGWAAAAFAVLTAYY136 pKa = 10.13 VRR138 pKa = 11.84 EE139 pKa = 4.14 LGRR142 pKa = 11.84 ANGAPSDD149 pKa = 4.12 FSGPMAKK156 pKa = 7.72 QHH158 pKa = 5.49 RR159 pKa = 11.84 MAVITAAALFSAVEE173 pKa = 4.12 FLWHH177 pKa = 6.23 GRR179 pKa = 11.84 NEE181 pKa = 4.25 VLTLALSIVAIGASLTAFRR200 pKa = 11.84 RR201 pKa = 11.84 ARR203 pKa = 11.84 RR204 pKa = 11.84 QVRR207 pKa = 11.84 WLKK210 pKa = 9.88 EE211 pKa = 3.66 RR212 pKa = 11.84 KK213 pKa = 8.84 AWQSTPP219 pKa = 3.1
Molecular weight: 23.3 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.347
IPC2_protein 9.531
IPC_protein 10.452
Toseland 10.789
ProMoST 10.57
Dawson 10.847
Bjellqvist 10.599
Wikipedia 11.096
Rodwell 10.95
Grimsley 10.877
Solomon 11.038
Lehninger 10.994
Nozaki 10.774
DTASelect 10.584
Thurlkill 10.774
EMBOSS 11.199
Sillero 10.789
Patrickios 10.701
IPC_peptide 11.038
IPC2_peptide 9.721
IPC2.peptide.svr19 8.761
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
6444
0
6444
2014053
26
7420
312.5
33.92
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
12.005 ± 0.04
0.786 ± 0.009
5.633 ± 0.031
5.705 ± 0.032
3.951 ± 0.022
8.592 ± 0.039
1.953 ± 0.016
5.633 ± 0.027
3.742 ± 0.026
9.868 ± 0.044
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.56 ± 0.017
2.835 ± 0.025
4.976 ± 0.022
2.988 ± 0.016
6.561 ± 0.036
5.725 ± 0.025
5.421 ± 0.046
7.466 ± 0.025
1.314 ± 0.012
2.286 ± 0.015
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here