Wenling narna-like virus 4

Taxonomy: Viruses; Riboviria; unclassified Riboviria; unclassified RNA viruses ShiM-2016

Average proteome isoelectric point is 7.97

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 1 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A1L3KIV8|A0A1L3KIV8_9VIRU RNA-dependent RNA polymerase OS=Wenling narna-like virus 4 OX=1923504 PE=4 SV=1
MM1 pKa = 7.69AIINGTDD8 pKa = 3.28RR9 pKa = 11.84LHH11 pKa = 6.74EE12 pKa = 4.88PYY14 pKa = 10.34NSPIVGEE21 pKa = 4.15LDD23 pKa = 3.49SVEE26 pKa = 4.85KK27 pKa = 10.47IITTMGIWARR37 pKa = 11.84GFNLNIDD44 pKa = 4.55HH45 pKa = 7.37ISPVCRR51 pKa = 11.84TFNSFKK57 pKa = 11.11DD58 pKa = 3.34LTTSIKK64 pKa = 9.89EE65 pKa = 3.75WSNYY69 pKa = 9.39LMNCLVSNKK78 pKa = 9.59VIRR81 pKa = 11.84PSIKK85 pKa = 10.24KK86 pKa = 9.13PFNRR90 pKa = 11.84NLYY93 pKa = 9.9DD94 pKa = 3.57YY95 pKa = 11.04LRR97 pKa = 11.84RR98 pKa = 11.84ACYY101 pKa = 10.22NPSNTQRR108 pKa = 11.84CMIISLLMVKK118 pKa = 10.06KK119 pKa = 10.36GFPEE123 pKa = 4.17APKK126 pKa = 10.67CLVDD130 pKa = 3.78EE131 pKa = 4.39TLKK134 pKa = 10.97DD135 pKa = 3.69HH136 pKa = 7.21QEE138 pKa = 4.17LLSSPSEE145 pKa = 4.02RR146 pKa = 11.84TDD148 pKa = 2.86EE149 pKa = 4.19KK150 pKa = 10.62TVDD153 pKa = 4.61DD154 pKa = 4.06ILEE157 pKa = 4.35IVDD160 pKa = 4.01KK161 pKa = 10.61LFPYY165 pKa = 10.44GWDD168 pKa = 3.53LNKK171 pKa = 10.59GICGPLPTKK180 pKa = 10.34AVYY183 pKa = 9.89EE184 pKa = 4.42GGVATQYY191 pKa = 10.43VFPEE195 pKa = 4.5EE196 pKa = 4.34ACSLIQQAKK205 pKa = 7.83LTYY208 pKa = 10.55DD209 pKa = 3.47RR210 pKa = 11.84FLHH213 pKa = 5.22MRR215 pKa = 11.84KK216 pKa = 9.02HH217 pKa = 5.82FKK219 pKa = 10.33VKK221 pKa = 10.23AVAVQQPGKK230 pKa = 10.2IRR232 pKa = 11.84VITKK236 pKa = 9.72SEE238 pKa = 4.02YY239 pKa = 10.34SLKK242 pKa = 10.73CLMPLQNALMDD253 pKa = 3.73QLRR256 pKa = 11.84NNPSFCLTRR265 pKa = 11.84KK266 pKa = 9.26EE267 pKa = 4.29CEE269 pKa = 4.09EE270 pKa = 3.94NDD272 pKa = 2.97FDD274 pKa = 5.84IFLEE278 pKa = 4.19EE279 pKa = 3.78KK280 pKa = 10.44GAFVSGDD287 pKa = 3.55YY288 pKa = 10.79KK289 pKa = 11.18SATDD293 pKa = 4.17NLNLDD298 pKa = 3.51LQYY301 pKa = 11.4YY302 pKa = 9.0IMQRR306 pKa = 11.84ILFNSVTPWVKK317 pKa = 10.55SLWMVALRR325 pKa = 11.84EE326 pKa = 4.17VSSHH330 pKa = 5.89KK331 pKa = 10.12IEE333 pKa = 4.06YY334 pKa = 8.78EE335 pKa = 3.81KK336 pKa = 10.88LQSIDD341 pKa = 3.37QVRR344 pKa = 11.84GQLMGSLLSFPLLCIINYY362 pKa = 10.22SIFHH366 pKa = 6.59ILFPNKK372 pKa = 9.55KK373 pKa = 9.76VLINGDD379 pKa = 4.27DD380 pKa = 3.37ILFRR384 pKa = 11.84ASQQEE389 pKa = 3.77YY390 pKa = 10.74DD391 pKa = 3.2LWKK394 pKa = 10.94VKK396 pKa = 10.39VLSVGLQLSIGKK408 pKa = 9.69NYY410 pKa = 9.85FSNEE414 pKa = 3.12IFTINSKK421 pKa = 10.12FYY423 pKa = 10.25RR424 pKa = 11.84WQDD427 pKa = 2.96RR428 pKa = 11.84ANEE431 pKa = 3.76IGYY434 pKa = 9.13LHH436 pKa = 7.27LGHH439 pKa = 7.52LLGPMSGDD447 pKa = 3.14SYY449 pKa = 11.39RR450 pKa = 11.84RR451 pKa = 11.84IPQRR455 pKa = 11.84FQGMYY460 pKa = 10.17KK461 pKa = 10.11RR462 pKa = 11.84YY463 pKa = 8.51CQKK466 pKa = 10.32PFEE469 pKa = 4.23NLRR472 pKa = 11.84DD473 pKa = 3.9LLEE476 pKa = 4.23PTCFGGLGGLTLDD489 pKa = 4.78EE490 pKa = 4.49YY491 pKa = 11.62NKK493 pKa = 9.21TVPVRR498 pKa = 11.84RR499 pKa = 11.84PSALAIHH506 pKa = 6.47HH507 pKa = 5.38QMKK510 pKa = 7.72TQEE513 pKa = 4.17VIKK516 pKa = 10.46LGSLAGDD523 pKa = 3.5VLDD526 pKa = 4.61FMSFKK531 pKa = 10.63FMTWIYY537 pKa = 8.78PSCRR541 pKa = 11.84RR542 pKa = 11.84EE543 pKa = 4.7DD544 pKa = 3.15IPQRR548 pKa = 11.84TRR550 pKa = 11.84IRR552 pKa = 11.84TKK554 pKa = 10.59GKK556 pKa = 10.16LGPLYY561 pKa = 9.36EE562 pKa = 4.06PRR564 pKa = 11.84MIEE567 pKa = 3.67RR568 pKa = 11.84TGNFMTFIGYY578 pKa = 9.34

Molecular weight: 66.92 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A1L3KIV8|A0A1L3KIV8_9VIRU RNA-dependent RNA polymerase OS=Wenling narna-like virus 4 OX=1923504 PE=4 SV=1
MM1 pKa = 7.69AIINGTDD8 pKa = 3.28RR9 pKa = 11.84LHH11 pKa = 6.74EE12 pKa = 4.88PYY14 pKa = 10.34NSPIVGEE21 pKa = 4.15LDD23 pKa = 3.49SVEE26 pKa = 4.85KK27 pKa = 10.47IITTMGIWARR37 pKa = 11.84GFNLNIDD44 pKa = 4.55HH45 pKa = 7.37ISPVCRR51 pKa = 11.84TFNSFKK57 pKa = 11.11DD58 pKa = 3.34LTTSIKK64 pKa = 9.89EE65 pKa = 3.75WSNYY69 pKa = 9.39LMNCLVSNKK78 pKa = 9.59VIRR81 pKa = 11.84PSIKK85 pKa = 10.24KK86 pKa = 9.13PFNRR90 pKa = 11.84NLYY93 pKa = 9.9DD94 pKa = 3.57YY95 pKa = 11.04LRR97 pKa = 11.84RR98 pKa = 11.84ACYY101 pKa = 10.22NPSNTQRR108 pKa = 11.84CMIISLLMVKK118 pKa = 10.06KK119 pKa = 10.36GFPEE123 pKa = 4.17APKK126 pKa = 10.67CLVDD130 pKa = 3.78EE131 pKa = 4.39TLKK134 pKa = 10.97DD135 pKa = 3.69HH136 pKa = 7.21QEE138 pKa = 4.17LLSSPSEE145 pKa = 4.02RR146 pKa = 11.84TDD148 pKa = 2.86EE149 pKa = 4.19KK150 pKa = 10.62TVDD153 pKa = 4.61DD154 pKa = 4.06ILEE157 pKa = 4.35IVDD160 pKa = 4.01KK161 pKa = 10.61LFPYY165 pKa = 10.44GWDD168 pKa = 3.53LNKK171 pKa = 10.59GICGPLPTKK180 pKa = 10.34AVYY183 pKa = 9.89EE184 pKa = 4.42GGVATQYY191 pKa = 10.43VFPEE195 pKa = 4.5EE196 pKa = 4.34ACSLIQQAKK205 pKa = 7.83LTYY208 pKa = 10.55DD209 pKa = 3.47RR210 pKa = 11.84FLHH213 pKa = 5.22MRR215 pKa = 11.84KK216 pKa = 9.02HH217 pKa = 5.82FKK219 pKa = 10.33VKK221 pKa = 10.23AVAVQQPGKK230 pKa = 10.2IRR232 pKa = 11.84VITKK236 pKa = 9.72SEE238 pKa = 4.02YY239 pKa = 10.34SLKK242 pKa = 10.73CLMPLQNALMDD253 pKa = 3.73QLRR256 pKa = 11.84NNPSFCLTRR265 pKa = 11.84KK266 pKa = 9.26EE267 pKa = 4.29CEE269 pKa = 4.09EE270 pKa = 3.94NDD272 pKa = 2.97FDD274 pKa = 5.84IFLEE278 pKa = 4.19EE279 pKa = 3.78KK280 pKa = 10.44GAFVSGDD287 pKa = 3.55YY288 pKa = 10.79KK289 pKa = 11.18SATDD293 pKa = 4.17NLNLDD298 pKa = 3.51LQYY301 pKa = 11.4YY302 pKa = 9.0IMQRR306 pKa = 11.84ILFNSVTPWVKK317 pKa = 10.55SLWMVALRR325 pKa = 11.84EE326 pKa = 4.17VSSHH330 pKa = 5.89KK331 pKa = 10.12IEE333 pKa = 4.06YY334 pKa = 8.78EE335 pKa = 3.81KK336 pKa = 10.88LQSIDD341 pKa = 3.37QVRR344 pKa = 11.84GQLMGSLLSFPLLCIINYY362 pKa = 10.22SIFHH366 pKa = 6.59ILFPNKK372 pKa = 9.55KK373 pKa = 9.76VLINGDD379 pKa = 4.27DD380 pKa = 3.37ILFRR384 pKa = 11.84ASQQEE389 pKa = 3.77YY390 pKa = 10.74DD391 pKa = 3.2LWKK394 pKa = 10.94VKK396 pKa = 10.39VLSVGLQLSIGKK408 pKa = 9.69NYY410 pKa = 9.85FSNEE414 pKa = 3.12IFTINSKK421 pKa = 10.12FYY423 pKa = 10.25RR424 pKa = 11.84WQDD427 pKa = 2.96RR428 pKa = 11.84ANEE431 pKa = 3.76IGYY434 pKa = 9.13LHH436 pKa = 7.27LGHH439 pKa = 7.52LLGPMSGDD447 pKa = 3.14SYY449 pKa = 11.39RR450 pKa = 11.84RR451 pKa = 11.84IPQRR455 pKa = 11.84FQGMYY460 pKa = 10.17KK461 pKa = 10.11RR462 pKa = 11.84YY463 pKa = 8.51CQKK466 pKa = 10.32PFEE469 pKa = 4.23NLRR472 pKa = 11.84DD473 pKa = 3.9LLEE476 pKa = 4.23PTCFGGLGGLTLDD489 pKa = 4.78EE490 pKa = 4.49YY491 pKa = 11.62NKK493 pKa = 9.21TVPVRR498 pKa = 11.84RR499 pKa = 11.84PSALAIHH506 pKa = 6.47HH507 pKa = 5.38QMKK510 pKa = 7.72TQEE513 pKa = 4.17VIKK516 pKa = 10.46LGSLAGDD523 pKa = 3.5VLDD526 pKa = 4.61FMSFKK531 pKa = 10.63FMTWIYY537 pKa = 8.78PSCRR541 pKa = 11.84RR542 pKa = 11.84EE543 pKa = 4.7DD544 pKa = 3.15IPQRR548 pKa = 11.84TRR550 pKa = 11.84IRR552 pKa = 11.84TKK554 pKa = 10.59GKK556 pKa = 10.16LGPLYY561 pKa = 9.36EE562 pKa = 4.06PRR564 pKa = 11.84MIEE567 pKa = 3.67RR568 pKa = 11.84TGNFMTFIGYY578 pKa = 9.34

Molecular weight: 66.92 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

1

0

1

578

578

578

578.0

66.92

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

3.287 ± 0.0

2.422 ± 0.0

5.19 ± 0.0

5.536 ± 0.0

5.017 ± 0.0

5.709 ± 0.0

1.903 ± 0.0

7.266 ± 0.0

7.093 ± 0.0

10.9 ± 0.0

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

3.114 ± 0.0

5.19 ± 0.0

5.017 ± 0.0

4.152 ± 0.0

5.882 ± 0.0

6.574 ± 0.0

4.671 ± 0.0

5.19 ± 0.0

1.384 ± 0.0

4.498 ± 0.0
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski