Bordetella genomosp. 5

Taxonomy: cellular organisms; Bacteria; Proteobacteria; Betaproteobacteria; Burkholderiales; Alcaligenaceae; Bordetella

Average proteome isoelectric point is 6.78

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 4667 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A261TCN7|A0A261TCN7_9BORD UDP-N-acetylmuramate--L-alanine ligase OS=Bordetella genomosp. 5 OX=1395608 GN=murC PE=3 SV=1
MM1 pKa = 7.62AALDD5 pKa = 3.49QALNNDD11 pKa = 3.42VAAIDD16 pKa = 4.03LNEE19 pKa = 3.97VADD22 pKa = 4.43RR23 pKa = 11.84LDD25 pKa = 3.52VLEE28 pKa = 4.65SALAPTCVEE37 pKa = 3.77LRR39 pKa = 11.84EE40 pKa = 4.02MSEE43 pKa = 4.01LQSVIRR49 pKa = 11.84QTAAIDD55 pKa = 3.36ACDD58 pKa = 3.47YY59 pKa = 7.21FTPVMYY65 pKa = 10.58RR66 pKa = 11.84HH67 pKa = 6.71DD68 pKa = 4.48RR69 pKa = 11.84LPEE72 pKa = 4.11AVSSLVDD79 pKa = 3.32VCEE82 pKa = 3.98VTFFDD87 pKa = 3.38TTYY90 pKa = 10.35YY91 pKa = 11.3ALSTVAAA98 pKa = 4.58

Molecular weight: 10.78 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A261U0H5|A0A261U0H5_9BORD TonB-dependent receptor OS=Bordetella genomosp. 5 OX=1395608 GN=CAL25_03365 PE=3 SV=1
MM1 pKa = 7.35KK2 pKa = 9.36RR3 pKa = 11.84TYY5 pKa = 10.06QPSVTRR11 pKa = 11.84RR12 pKa = 11.84KK13 pKa = 8.26RR14 pKa = 11.84THH16 pKa = 5.86GFRR19 pKa = 11.84LRR21 pKa = 11.84MKK23 pKa = 9.06TRR25 pKa = 11.84AGRR28 pKa = 11.84AILNARR34 pKa = 11.84RR35 pKa = 11.84AKK37 pKa = 9.68GRR39 pKa = 11.84KK40 pKa = 8.75RR41 pKa = 11.84LAVV44 pKa = 3.41

Molecular weight: 5.25 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

4667

0

4667

1552314

29

2787

332.6

35.97

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

13.406 ± 0.051

0.837 ± 0.01

5.434 ± 0.027

5.023 ± 0.035

3.296 ± 0.021

8.54 ± 0.05

2.158 ± 0.017

4.305 ± 0.025

2.619 ± 0.032

10.822 ± 0.047

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.4 ± 0.017

2.482 ± 0.02

5.475 ± 0.031

3.891 ± 0.024

7.365 ± 0.036

5.167 ± 0.035

5.255 ± 0.034

7.705 ± 0.031

1.446 ± 0.015

2.375 ± 0.02
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski