Diodia vein chlorosis virus
Average proteome isoelectric point is 6.62
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 10 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|E7BKK0|E7BKK0_9CLOS p60 OS=Diodia vein chlorosis virus OX=656520 PE=4 SV=1
MM1 pKa = 7.41 ALVVLLLLEE10 pKa = 4.16 MFGGFNSYY18 pKa = 10.55 YY19 pKa = 10.76 LSDD22 pKa = 3.5 NSSFSGQILVIRR34 pKa = 11.84 TEE36 pKa = 4.08 DD37 pKa = 3.52 PKK39 pKa = 11.5 LLTDD43 pKa = 4.32 ILLEE47 pKa = 3.86 LPYY50 pKa = 10.37 IKK52 pKa = 10.35 EE53 pKa = 3.8 RR54 pKa = 11.84 WW55 pKa = 3.38
Molecular weight: 6.37 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 4.529
IPC2_protein 4.622
IPC_protein 4.329
Toseland 4.177
ProMoST 4.457
Dawson 4.279
Bjellqvist 4.444
Wikipedia 4.177
Rodwell 4.164
Grimsley 4.088
Solomon 4.266
Lehninger 4.215
Nozaki 4.418
DTASelect 4.533
Thurlkill 4.202
EMBOSS 4.19
Sillero 4.444
Patrickios 3.719
IPC_peptide 4.266
IPC2_peptide 4.418
IPC2.peptide.svr19 4.406
Protein with the highest isoelectric point:
>tr|E7BKJ9|E7BKJ9_9CLOS p6 OS=Diodia vein chlorosis virus OX=656520 PE=4 SV=1
MM1 pKa = 6.94 NTDD4 pKa = 3.27 ILISCLEE11 pKa = 3.94 KK12 pKa = 11.04 FNINKK17 pKa = 9.12 NFSFEE22 pKa = 4.39 NYY24 pKa = 9.17 GSQGRR29 pKa = 11.84 FRR31 pKa = 11.84 FWYY34 pKa = 9.76 NIQYY38 pKa = 9.87 YY39 pKa = 10.75 LKK41 pKa = 10.63 LCC43 pKa = 4.27
Molecular weight: 5.32 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 8.454
IPC2_protein 7.819
IPC_protein 8.126
Toseland 7.849
ProMoST 8.346
Dawson 8.492
Bjellqvist 8.712
Wikipedia 8.448
Rodwell 8.463
Grimsley 7.454
Solomon 8.858
Lehninger 8.858
Nozaki 8.843
DTASelect 8.507
Thurlkill 8.536
EMBOSS 8.726
Sillero 8.858
Patrickios 2.193
IPC_peptide 8.843
IPC2_peptide 7.966
IPC2.peptide.svr19 7.955
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
10
0
10
6925
43
2505
692.5
79.59
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
3.812 ± 0.272
2.094 ± 0.242
6.801 ± 0.196
5.805 ± 0.313
5.762 ± 0.248
4.765 ± 0.237
1.314 ± 0.135
7.032 ± 0.249
8.953 ± 0.296
8.982 ± 0.198
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.599 ± 0.213
6.469 ± 0.504
3.018 ± 0.201
2.671 ± 0.142
4.635 ± 0.138
7.841 ± 0.344
4.996 ± 0.297
7.206 ± 0.242
0.664 ± 0.066
4.578 ± 0.182
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here