Desulfobacteraceae bacterium SEEP-SAG9

Taxonomy: cellular organisms; Bacteria; Proteobacteria; delta/epsilon subdivisions; Deltaproteobacteria; Desulfobacterales; Desulfobacteraceae; unclassified Desulfobacteraceae

Average proteome isoelectric point is 6.89

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 2621 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A2S8K856|A0A2S8K856_9DELT GMC_oxred_C domain-containing protein OS=Desulfobacteraceae bacterium SEEP-SAG9 OX=2100161 GN=C6A37_03145 PE=4 SV=1
MM1 pKa = 7.27KK2 pKa = 9.68TRR4 pKa = 11.84LVINMLFALLFFISGVSAQTTYY26 pKa = 10.54IVAPSGGDD34 pKa = 3.09FTTIQDD40 pKa = 4.79CINGAINGDD49 pKa = 3.17ICLISPGVYY58 pKa = 9.71VEE60 pKa = 4.91NIDD63 pKa = 5.04FIGKK67 pKa = 8.71NLTVKK72 pKa = 10.53SSSGPEE78 pKa = 3.5ATSIDD83 pKa = 3.96GADD86 pKa = 3.43SGSVVRR92 pKa = 11.84FQTNEE97 pKa = 3.53TAEE100 pKa = 4.74AILDD104 pKa = 3.5GFMIRR109 pKa = 11.84NGNDD113 pKa = 3.15LQGGGIFCQGSSPTIKK129 pKa = 10.58NCIITEE135 pKa = 4.03NEE137 pKa = 4.02VYY139 pKa = 10.59GIEE142 pKa = 4.34GSDD145 pKa = 3.72GEE147 pKa = 4.8GAGIYY152 pKa = 10.05CNNSSPRR159 pKa = 11.84IMDD162 pKa = 3.23CTISYY167 pKa = 10.58NIAAVSDD174 pKa = 3.53YY175 pKa = 10.93GYY177 pKa = 11.04AYY179 pKa = 10.46AHH181 pKa = 6.78GGGIYY186 pKa = 10.11SVQSSSTFTDD196 pKa = 3.43CTITHH201 pKa = 6.47NEE203 pKa = 3.62AGTFGGGISCWDD215 pKa = 3.49SSSSTFTDD223 pKa = 4.75CIISNNSALGRR234 pKa = 11.84GGGGIYY240 pKa = 10.28SSSTLMIEE248 pKa = 4.01RR249 pKa = 11.84CTVSDD254 pKa = 3.38NYY256 pKa = 10.87AYY258 pKa = 10.28QGGGIYY264 pKa = 9.99SYY266 pKa = 11.91GEE268 pKa = 4.0TTISDD273 pKa = 4.01SIIAGNSSDD282 pKa = 4.83DD283 pKa = 3.76DD284 pKa = 4.37GGGIFSYY291 pKa = 11.38GNILSLVNCTIFNNSGNVGGGVCFKK316 pKa = 10.6GDD318 pKa = 3.99CNATIANSIIADD330 pKa = 3.53NTGNCGGGIAVWGSPSGPGSFSITEE355 pKa = 4.07STISGNTASGCGGGFWFDD373 pKa = 3.75YY374 pKa = 11.05VPLATVTNCMIFRR387 pKa = 11.84NTALGGGGGGGNITGEE403 pKa = 4.37TSTMINSSTFFGNSSTEE420 pKa = 3.72VGGGIFYY427 pKa = 8.93RR428 pKa = 11.84TDD430 pKa = 3.32GDD432 pKa = 3.8NVSSFNVIKK441 pKa = 10.91NCILWDD447 pKa = 4.08DD448 pKa = 4.13SATDD452 pKa = 4.04GPEE455 pKa = 3.42IALVEE460 pKa = 4.37EE461 pKa = 4.64YY462 pKa = 10.96GDD464 pKa = 4.03PEE466 pKa = 4.62LSVEE470 pKa = 4.05MSYY473 pKa = 11.72SDD475 pKa = 3.63VKK477 pKa = 11.0GGQSDD482 pKa = 3.33VHH484 pKa = 8.22IIGGVLNWQAGNIEE498 pKa = 4.64SNPLFVDD505 pKa = 3.59PDD507 pKa = 3.94GDD509 pKa = 4.32DD510 pKa = 3.99NLPGTLDD517 pKa = 4.19DD518 pKa = 5.56DD519 pKa = 4.24FHH521 pKa = 6.42LTSDD525 pKa = 4.38SLCIDD530 pKa = 4.01AGTSQDD536 pKa = 4.01APSIDD541 pKa = 3.27IDD543 pKa = 4.19GDD545 pKa = 3.68PRR547 pKa = 11.84PQGAAIDD554 pKa = 3.96IGADD558 pKa = 3.52EE559 pKa = 5.38YY560 pKa = 10.98IDD562 pKa = 4.6PDD564 pKa = 3.66SDD566 pKa = 3.85GDD568 pKa = 4.14GIEE571 pKa = 4.06YY572 pKa = 10.55GIDD575 pKa = 3.06IDD577 pKa = 3.85PTVFSSEE584 pKa = 3.92FSDD587 pKa = 5.25AEE589 pKa = 4.09TTYY592 pKa = 11.22GIIIDD597 pKa = 4.48RR598 pKa = 11.84GDD600 pKa = 3.45QVVTVIDD607 pKa = 4.18LPDD610 pKa = 4.37PNGVRR615 pKa = 11.84IAASGGDD622 pKa = 3.65TPASVSACGGSAIFTLNDD640 pKa = 3.08GDD642 pKa = 5.12VITFTCGSVKK652 pKa = 10.04IEE654 pKa = 4.34VISGPVKK661 pKa = 10.24ISFVADD667 pKa = 3.89DD668 pKa = 4.15GTTATTTLDD677 pKa = 3.26AGNILTFEE685 pKa = 4.7PEE687 pKa = 4.15TLIITAPDD695 pKa = 3.64TNTEE699 pKa = 3.85NAVIVVDD706 pKa = 4.21GKK708 pKa = 11.06EE709 pKa = 3.81FAIEE713 pKa = 3.99PGEE716 pKa = 4.24SVEE719 pKa = 4.27LDD721 pKa = 2.98ITIVIDD727 pKa = 4.07GCDD730 pKa = 3.46TGVLDD735 pKa = 6.02SDD737 pKa = 5.11YY738 pKa = 10.45EE739 pKa = 4.14DD740 pKa = 3.71QSISEE745 pKa = 4.77WISQCAEE752 pKa = 3.49NAKK755 pKa = 10.02NHH757 pKa = 5.21GKK759 pKa = 10.04YY760 pKa = 10.01VSCVAKK766 pKa = 9.27LTKK769 pKa = 9.88KK770 pKa = 10.12LKK772 pKa = 9.47KK773 pKa = 10.43AGVITGKK780 pKa = 10.4EE781 pKa = 3.48KK782 pKa = 10.98GAIQSCAAQADD793 pKa = 3.61IHH795 pKa = 6.3

Molecular weight: 82.17 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A2S8K265|A0A2S8K265_9DELT Uncharacterized protein (Fragment) OS=Desulfobacteraceae bacterium SEEP-SAG9 OX=2100161 GN=C6A37_13455 PE=4 SV=1
MM1 pKa = 7.35KK2 pKa = 9.4RR3 pKa = 11.84TYY5 pKa = 9.91QPSRR9 pKa = 11.84IKK11 pKa = 10.5RR12 pKa = 11.84ARR14 pKa = 11.84THH16 pKa = 6.4GFLKK20 pKa = 10.61RR21 pKa = 11.84MSTKK25 pKa = 9.49QGRR28 pKa = 11.84RR29 pKa = 11.84IINKK33 pKa = 8.92RR34 pKa = 11.84RR35 pKa = 11.84AKK37 pKa = 9.48GRR39 pKa = 11.84KK40 pKa = 8.79RR41 pKa = 11.84LTVV44 pKa = 3.11

Molecular weight: 5.37 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

2621

0

2621

550168

27

1467

209.9

23.54

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

7.528 ± 0.054

1.261 ± 0.023

5.392 ± 0.038

6.46 ± 0.066

4.61 ± 0.039

7.149 ± 0.062

2.095 ± 0.025

7.733 ± 0.051

6.663 ± 0.062

9.861 ± 0.058

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.604 ± 0.028

3.977 ± 0.033

4.29 ± 0.038

3.232 ± 0.029

5.363 ± 0.047

5.749 ± 0.036

5.048 ± 0.037

6.643 ± 0.04

1.148 ± 0.025

3.193 ± 0.031
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski