Lachnospiraceae bacterium AM48-27BH

Taxonomy: cellular organisms; Bacteria; Terrabacteria group; Firmicutes; Clostridia; Eubacteriales; Lachnospiraceae; unclassified Lachnospiraceae

Average proteome isoelectric point is 6.18

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 3679 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A416JS24|A0A416JS24_9FIRM Cobalt-precorrin-5B C(1)-methyltransferase OS=Lachnospiraceae bacterium AM48-27BH OX=2292271 GN=cbiD PE=3 SV=1
MM1 pKa = 7.17VRR3 pKa = 11.84LKK5 pKa = 10.84KK6 pKa = 10.58IFDD9 pKa = 4.2LLLATVLCLSLSACGSSDD27 pKa = 3.22TDD29 pKa = 3.63TEE31 pKa = 4.48GAGDD35 pKa = 3.61DD36 pKa = 3.6WRR38 pKa = 11.84NSGVVVGSGTITHH51 pKa = 6.08TNEE54 pKa = 3.48GSVDD58 pKa = 3.61VLVTVSPEE66 pKa = 3.43SAAFYY71 pKa = 10.14RR72 pKa = 11.84DD73 pKa = 3.17KK74 pKa = 11.46AEE76 pKa = 4.16QILFDD81 pKa = 3.94SVSFPVNIPDD91 pKa = 4.65AEE93 pKa = 4.2QAFNAISFDD102 pKa = 4.07DD103 pKa = 3.61MDD105 pKa = 6.25GDD107 pKa = 4.24GEE109 pKa = 4.67SDD111 pKa = 3.64VLVSFIHH118 pKa = 6.2EE119 pKa = 4.4NGDD122 pKa = 3.08ITEE125 pKa = 5.73LIWIWDD131 pKa = 3.48SAEE134 pKa = 3.64RR135 pKa = 11.84YY136 pKa = 8.92VFRR139 pKa = 11.84KK140 pKa = 10.0DD141 pKa = 3.35LSSVTISEE149 pKa = 4.85DD150 pKa = 3.3DD151 pKa = 3.32TAEE154 pKa = 5.01AIDD157 pKa = 3.93NSNLDD162 pKa = 3.72EE163 pKa = 4.78YY164 pKa = 11.41VGLWEE169 pKa = 4.75YY170 pKa = 11.3LDD172 pKa = 3.67EE173 pKa = 4.4NLWLRR178 pKa = 11.84IHH180 pKa = 7.32EE181 pKa = 4.47DD182 pKa = 3.48ATWEE186 pKa = 4.1FVNDD190 pKa = 3.29QDD192 pKa = 4.8EE193 pKa = 4.83VIEE196 pKa = 5.58SGTLWADD203 pKa = 3.34EE204 pKa = 4.45TGVTLHH210 pKa = 6.87FDD212 pKa = 3.24GSGDD216 pKa = 3.59VLQLDD221 pKa = 4.0RR222 pKa = 11.84TVSGDD227 pKa = 4.41LIDD230 pKa = 3.95IANDD234 pKa = 3.42GSLFPVEE241 pKa = 5.57AIQSSVPYY249 pKa = 7.87FTRR252 pKa = 11.84NGLEE256 pKa = 3.68INAAMDD262 pKa = 3.2MGTFLLEE269 pKa = 5.18DD270 pKa = 4.34GVCNYY275 pKa = 10.86SGLGDD280 pKa = 4.19GYY282 pKa = 11.5SRR284 pKa = 11.84DD285 pKa = 3.48DD286 pKa = 4.7CYY288 pKa = 11.29WEE290 pKa = 4.21VIKK293 pKa = 11.13NADD296 pKa = 3.6YY297 pKa = 9.07THH299 pKa = 7.17DD300 pKa = 4.91GIRR303 pKa = 11.84EE304 pKa = 3.92IQFDD308 pKa = 4.9AICYY312 pKa = 9.23IPEE315 pKa = 3.77SSIPYY320 pKa = 9.62FDD322 pKa = 3.29QQYY325 pKa = 7.95FTVTSSEE332 pKa = 4.38LYY334 pKa = 10.79DD335 pKa = 4.25FNTGMWLTAASAYY348 pKa = 10.52DD349 pKa = 3.4NSNRR353 pKa = 11.84GEE355 pKa = 4.02NYY357 pKa = 10.11YY358 pKa = 10.98LHH360 pKa = 5.72TVSWKK365 pKa = 9.42GNSYY369 pKa = 10.62LIEE372 pKa = 4.14FAYY375 pKa = 9.29STDD378 pKa = 2.58WTYY381 pKa = 11.85DD382 pKa = 3.13VGDD385 pKa = 3.7WDD387 pKa = 3.95MVLTKK392 pKa = 10.46SYY394 pKa = 10.65IVYY397 pKa = 10.15LPEE400 pKa = 6.32DD401 pKa = 3.93MTASSSPQKK410 pKa = 10.58YY411 pKa = 9.05SRR413 pKa = 11.84TIIKK417 pKa = 9.69IAPRR421 pKa = 11.84EE422 pKa = 3.96CSLTAFHH429 pKa = 7.31PKK431 pKa = 9.25QALL434 pKa = 3.28

Molecular weight: 48.64 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A416JKH7|A0A416JKH7_9FIRM Chromosome segregation protein SMC OS=Lachnospiraceae bacterium AM48-27BH OX=2292271 GN=DW974_16750 PE=4 SV=1
MM1 pKa = 7.67KK2 pKa = 8.72MTFQPKK8 pKa = 8.95KK9 pKa = 7.58RR10 pKa = 11.84QRR12 pKa = 11.84SKK14 pKa = 9.17VHH16 pKa = 5.89GFRR19 pKa = 11.84ARR21 pKa = 11.84MSTPGGRR28 pKa = 11.84KK29 pKa = 8.8VLAARR34 pKa = 11.84RR35 pKa = 11.84AKK37 pKa = 10.33GRR39 pKa = 11.84AKK41 pKa = 10.69LSAA44 pKa = 3.92

Molecular weight: 4.98 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

3679

0

3679

1102997

29

4769

299.8

33.69

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

7.606 ± 0.053

1.499 ± 0.021

5.636 ± 0.031

7.519 ± 0.053

4.015 ± 0.029

7.126 ± 0.049

1.879 ± 0.021

6.845 ± 0.039

6.595 ± 0.04

8.906 ± 0.046

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

3.136 ± 0.024

4.118 ± 0.032

3.467 ± 0.029

3.67 ± 0.027

4.641 ± 0.043

5.766 ± 0.043

5.537 ± 0.048

6.734 ± 0.037

1.112 ± 0.017

4.193 ± 0.029
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski