Intrasporangium calvum (strain ATCC 23552 / DSM 43043 / JCM 3097 / NBRC 12989 / 7 KIP)

Taxonomy: cellular organisms; Bacteria; Terrabacteria group; Actinobacteria; Actinomycetia; Micrococcales; Intrasporangiaceae; Intrasporangium; Intrasporangium calvum

Average proteome isoelectric point is 6.23

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 3551 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|E6S6R1|E6S6R1_INTC7 Cell wall binding repeat 2-containing protein OS=Intrasporangium calvum (strain ATCC 23552 / DSM 43043 / JCM 3097 / NBRC 12989 / 7 KIP) OX=710696 GN=Intca_0240 PE=4 SV=1
MM1 pKa = 7.24KK2 pKa = 10.62VSGRR6 pKa = 11.84LAAALLAAVTTVAVGGSPAVAAPPTNDD33 pKa = 2.79MYY35 pKa = 11.61AGAEE39 pKa = 4.22AIAALPFATTLDD51 pKa = 3.73TTEE54 pKa = 4.24ATTDD58 pKa = 3.51ADD60 pKa = 3.89DD61 pKa = 5.24AEE63 pKa = 5.45LNGQCGAPAMDD74 pKa = 3.48ASVWYY79 pKa = 10.09SYY81 pKa = 8.8TAEE84 pKa = 4.48ADD86 pKa = 3.77SLLLADD92 pKa = 5.04AFEE95 pKa = 5.19SDD97 pKa = 3.77YY98 pKa = 11.6SAGVLVATGAPGNFLVQGCGPGGAGWEE125 pKa = 4.08AMAGEE130 pKa = 4.97TYY132 pKa = 10.63YY133 pKa = 10.29IVVFDD138 pKa = 4.53DD139 pKa = 4.18QEE141 pKa = 6.37DD142 pKa = 4.26GTGNGGTMSLTVDD155 pKa = 3.97VAPPAPAIEE164 pKa = 4.24ATVNPLGWFDD174 pKa = 3.87ARR176 pKa = 11.84TGFATISGTVTCDD189 pKa = 2.87SSADD193 pKa = 3.58FAFVDD198 pKa = 3.48VTLQQRR204 pKa = 11.84VGRR207 pKa = 11.84FIVRR211 pKa = 11.84GYY213 pKa = 10.83GGTEE217 pKa = 4.01VFCDD221 pKa = 3.99GTPQPWSAEE230 pKa = 3.77ISGEE234 pKa = 3.95TGLFKK239 pKa = 10.72GGRR242 pKa = 11.84ALSVSFSVACNSGGCSEE259 pKa = 4.73YY260 pKa = 10.61FDD262 pKa = 3.77EE263 pKa = 4.98TEE265 pKa = 4.14VRR267 pKa = 11.84LTGKK271 pKa = 10.02KK272 pKa = 8.96RR273 pKa = 3.18

Molecular weight: 27.86 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|E6SDF0|E6SDF0_INTC7 Uncharacterized protein OS=Intrasporangium calvum (strain ATCC 23552 / DSM 43043 / JCM 3097 / NBRC 12989 / 7 KIP) OX=710696 GN=Intca_1049 PE=4 SV=1
MM1 pKa = 8.09RR2 pKa = 11.84LRR4 pKa = 11.84SSLVLARR11 pKa = 11.84MLLRR15 pKa = 11.84FFIRR19 pKa = 11.84RR20 pKa = 11.84VTRR23 pKa = 11.84MGVLRR28 pKa = 11.84STAVKK33 pKa = 9.81VAHH36 pKa = 6.12RR37 pKa = 11.84TT38 pKa = 3.49

Molecular weight: 4.5 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

3551

0

3551

1195564

31

3498

336.7

36.02

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

13.123 ± 0.058

0.669 ± 0.011

6.145 ± 0.037

5.651 ± 0.039

2.774 ± 0.023

9.278 ± 0.033

2.293 ± 0.02

3.676 ± 0.028

1.935 ± 0.031

10.217 ± 0.046

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

1.81 ± 0.015

1.72 ± 0.022

5.805 ± 0.033

2.737 ± 0.021

7.875 ± 0.045

5.459 ± 0.029

6.097 ± 0.035

9.38 ± 0.04

1.513 ± 0.021

1.845 ± 0.018
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski