Corynebacterium doosanense CAU 212 = DSM 45436
Average proteome isoelectric point is 5.76
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 2476 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A097IJK7|A0A097IJK7_9CORY Uncharacterized protein OS=Corynebacterium doosanense CAU 212 = DSM 45436 OX=558173 GN=CDOO_12050 PE=4 SV=1
MM1 pKa = 7.52 SKK3 pKa = 10.22 FAVAAVIATPLVLVACGSGDD23 pKa = 3.97 DD24 pKa = 4.31 VEE26 pKa = 4.75 EE27 pKa = 4.18 QAASASTVTSQATAASTTQTSSTTSASTSASEE59 pKa = 4.27 SAEE62 pKa = 3.87 GEE64 pKa = 4.51 TPSAAADD71 pKa = 3.72 QPSGSAADD79 pKa = 3.85 GGAAPVLEE87 pKa = 4.3 NPFEE91 pKa = 4.6 NGDD94 pKa = 3.72 IEE96 pKa = 4.69 VAQPDD101 pKa = 4.21 PVQGRR106 pKa = 11.84 PASAEE111 pKa = 3.84 EE112 pKa = 4.33 TEE114 pKa = 4.23 ALSNLVNGIYY124 pKa = 9.1 QQPSLHH130 pKa = 6.24 GFMMYY135 pKa = 9.57 MPNHH139 pKa = 5.81 TCAATLEE146 pKa = 4.25 AQGGIEE152 pKa = 4.73 AYY154 pKa = 10.34 SLDD157 pKa = 5.05 GIPDD161 pKa = 3.27 MPMNQFPGYY170 pKa = 9.47 SEE172 pKa = 4.24 SRR174 pKa = 11.84 VTAIEE179 pKa = 4.04 NQTVTDD185 pKa = 3.65 SGDD188 pKa = 3.36 RR189 pKa = 11.84 ASATVTVQSAEE200 pKa = 4.24 GPSTGTMVFQHH211 pKa = 6.69 EE212 pKa = 4.15 GGEE215 pKa = 4.25 WKK217 pKa = 10.43 FCSS220 pKa = 3.7
Molecular weight: 22.38 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.745
IPC2_protein 3.859
IPC_protein 3.795
Toseland 3.617
ProMoST 3.923
Dawson 3.757
Bjellqvist 3.91
Wikipedia 3.643
Rodwell 3.63
Grimsley 3.528
Solomon 3.745
Lehninger 3.694
Nozaki 3.872
DTASelect 4.012
Thurlkill 3.656
EMBOSS 3.656
Sillero 3.91
Patrickios 0.947
IPC_peptide 3.745
IPC2_peptide 3.897
IPC2.peptide.svr19 3.801
Protein with the highest isoelectric point:
>tr|A0A097IJ51|A0A097IJ51_9CORY Uncharacterized protein OS=Corynebacterium doosanense CAU 212 = DSM 45436 OX=558173 GN=CDOO_01860 PE=4 SV=1
MM1 pKa = 7.5 TKK3 pKa = 10.27 GKK5 pKa = 8.58 KK6 pKa = 7.73 TFQPNNRR13 pKa = 11.84 RR14 pKa = 11.84 RR15 pKa = 11.84 ARR17 pKa = 11.84 KK18 pKa = 8.46 HH19 pKa = 4.41 GFRR22 pKa = 11.84 IKK24 pKa = 9.68 MRR26 pKa = 11.84 TRR28 pKa = 11.84 AGRR31 pKa = 11.84 AIVAARR37 pKa = 11.84 RR38 pKa = 11.84 KK39 pKa = 9.72 KK40 pKa = 10.22 GRR42 pKa = 11.84 KK43 pKa = 9.08 SLTAA47 pKa = 4.07
Molecular weight: 5.49 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.521
IPC2_protein 11.257
IPC_protein 12.837
Toseland 13.013
ProMoST 13.495
Dawson 13.013
Bjellqvist 12.998
Wikipedia 13.481
Rodwell 12.749
Grimsley 13.056
Solomon 13.51
Lehninger 13.408
Nozaki 13.013
DTASelect 12.998
Thurlkill 13.013
EMBOSS 13.51
Sillero 13.013
Patrickios 12.486
IPC_peptide 13.51
IPC2_peptide 12.486
IPC2.peptide.svr19 9.165
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
2476
0
2476
780190
40
2999
315.1
34.01
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
11.958 ± 0.066
0.601 ± 0.013
6.363 ± 0.038
6.409 ± 0.047
3.172 ± 0.027
8.687 ± 0.042
2.101 ± 0.024
4.692 ± 0.033
2.543 ± 0.036
9.767 ± 0.05
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.135 ± 0.02
2.535 ± 0.029
5.138 ± 0.035
3.109 ± 0.027
6.726 ± 0.053
5.948 ± 0.035
6.219 ± 0.031
8.366 ± 0.04
1.408 ± 0.02
2.125 ± 0.022
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here