Haladaptatus litoreus
Average proteome isoelectric point is 5.12
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 4885 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A1N7BLR0|A0A1N7BLR0_9EURY Rad3-related DNA helicase OS=Haladaptatus litoreus OX=553468 GN=SAMN05421858_2626 PE=4 SV=1
MM1 pKa = 7.64 AIDD4 pKa = 3.84 PQFHH8 pKa = 6.32 EE9 pKa = 4.45 NRR11 pKa = 11.84 EE12 pKa = 4.15 KK13 pKa = 11.13 VDD15 pKa = 3.35 DD16 pKa = 4.37 HH17 pKa = 7.5 DD18 pKa = 4.59 GHH20 pKa = 6.87 DD21 pKa = 3.28 VWGPVDD27 pKa = 4.4 EE28 pKa = 4.58 PDD30 pKa = 3.33 KK31 pKa = 11.46 LGIHH35 pKa = 5.72 GTHH38 pKa = 5.93 VAVDD42 pKa = 4.39 FDD44 pKa = 4.39 LCIADD49 pKa = 4.45 GACLEE54 pKa = 4.62 DD55 pKa = 4.31 CPVDD59 pKa = 3.53 VFEE62 pKa = 4.76 WVDD65 pKa = 3.69 TPGHH69 pKa = 6.56 PEE71 pKa = 3.96 SEE73 pKa = 4.45 EE74 pKa = 3.97 KK75 pKa = 10.71 ADD77 pKa = 3.91 PANEE81 pKa = 4.02 AQCIDD86 pKa = 3.76 CMLCVDD92 pKa = 4.72 VCPVDD97 pKa = 5.72 AIDD100 pKa = 3.53 VDD102 pKa = 3.76 AGRR105 pKa = 11.84 VV106 pKa = 3.27
Molecular weight: 11.58 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.702
IPC2_protein 3.834
IPC_protein 3.834
Toseland 3.617
ProMoST 4.012
Dawson 3.834
Bjellqvist 3.999
Wikipedia 3.795
Rodwell 3.668
Grimsley 3.528
Solomon 3.834
Lehninger 3.783
Nozaki 3.961
DTASelect 4.215
Thurlkill 3.681
EMBOSS 3.795
Sillero 3.961
Patrickios 1.074
IPC_peptide 3.821
IPC2_peptide 3.935
IPC2.peptide.svr19 3.857
Protein with the highest isoelectric point:
>tr|A0A1N6X5Y0|A0A1N6X5Y0_9EURY PGF-CTERM protein/surface glycoprotein OS=Haladaptatus litoreus OX=553468 GN=SAMN05421858_0997 PE=3 SV=1
MM1 pKa = 7.52 LSILTPIRR9 pKa = 11.84 SVLRR13 pKa = 11.84 SIINVIIAIITLPLRR28 pKa = 11.84 ILRR31 pKa = 11.84 RR32 pKa = 11.84 LLL34 pKa = 3.68
Molecular weight: 3.89 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.386
IPC2_protein 10.818
IPC_protein 12.442
Toseland 12.603
ProMoST 13.1
Dawson 12.603
Bjellqvist 12.603
Wikipedia 13.086
Rodwell 12.106
Grimsley 12.647
Solomon 13.1
Lehninger 12.998
Nozaki 12.603
DTASelect 12.603
Thurlkill 12.603
EMBOSS 13.1
Sillero 12.603
Patrickios 11.901
IPC_peptide 13.1
IPC2_peptide 12.091
IPC2.peptide.svr19 9.16
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
4885
0
4885
1295187
29
1700
265.1
29.11
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
9.229 ± 0.043
0.793 ± 0.012
7.47 ± 0.049
8.41 ± 0.053
3.699 ± 0.027
8.106 ± 0.036
2.123 ± 0.021
4.863 ± 0.029
2.626 ± 0.023
8.849 ± 0.048
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
1.986 ± 0.017
3.124 ± 0.025
4.436 ± 0.023
2.948 ± 0.025
6.021 ± 0.038
6.249 ± 0.034
6.594 ± 0.03
8.405 ± 0.036
1.223 ± 0.015
2.847 ± 0.019
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here